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Hofmann1943
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Registered: 03/05/09
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A+,B+,(F+) VS Trichoderma
#13686884 - 12/26/10 02:37 PM (13 years, 6 months ago) |
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Mushrooms must prevail

both seem to go over trich and eat it how splendid.
-------------------- By Albert Hofmann : Been cautious man, I though I would start with a smallest, smallest quantity. Namely I started with 0.25mg.....and my intention was to increase dosage to see if something will happened. That very small dosage, the first dose of my experiments i planed, was very very strong.
Edited by Hofmann1943 (01/02/11 03:34 PM)
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pepper
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If I owned those Trich dishes I would be scared to open them. How are you going to isolate the good mycelium with out putting billions of Trich in the area that you are working in?
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audiophoenix
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Re: A+,B+ VS Trichoderma [Re: pepper]
#13686951 - 12/26/10 02:53 PM (13 years, 6 months ago) |
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I am gonna throw out the theory that there is something seriously wrong with how you are doing agar cultures...
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Edited by audiophoenix (12/26/10 02:54 PM)
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Hofmann1943
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Quote:
pepper said: If I owned those Trich dishes I would be scared to open them. How are you going to isolate the good mycelium with out putting billions of Trich in the area that you are working in?
I wont, I save them for experimental reasons, just to see if mushroom can beat mold.
Quote:
audiophoenix said: I am gonna throw out the theory that there is something seriously wrong with how you are doing agar cultures...
yeap this time i did it all wrong. Too much malt, pour in petri while too hot, no H2O2, and exposing agar dishes to contaminated air.
-------------------- By Albert Hofmann : Been cautious man, I though I would start with a smallest, smallest quantity. Namely I started with 0.25mg.....and my intention was to increase dosage to see if something will happened. That very small dosage, the first dose of my experiments i planed, was very very strong.
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audiophoenix
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I know there have been a few times where it looked like the mycelium had won and then a few days later I could see it was sick and dieing. Although it would be bad ass to have a strain of B+ that was trich resistant. This seems to be the Evil villin of Mycelium.
BTW... are we sure this is trich? I have seen mycelium concur a few molds but never trich.
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Hofmann1943
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i'm pretty sure, or maybe it is Aspergillus Flavus. I will post in couple of days.
-------------------- By Albert Hofmann : Been cautious man, I though I would start with a smallest, smallest quantity. Namely I started with 0.25mg.....and my intention was to increase dosage to see if something will happened. That very small dosage, the first dose of my experiments i planed, was very very strong.
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audiophoenix
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roaddog
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pepper
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Re: A+,B+ VS Trichoderma [Re: roaddog]
#13709124 - 12/30/10 11:07 PM (13 years, 5 months ago) |
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Hello Hofmann1943 I was wondering how thing where going and if you had any updated pictures please. Thanks.
Edited by pepper (12/30/10 11:08 PM)
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Hofmann1943
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Re: A+,B+ VS Trichoderma [Re: pepper]
#13710725 - 12/31/10 09:54 AM (13 years, 5 months ago) |
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Quote:
pepper said: Hello Hofmann1943 I was wondering how thing where going and if you had any updated pictures please. Thanks.
Well, experiment is not completed but here are some new pictures just because you asked. I have new candidate F+ 
-------------------- By Albert Hofmann : Been cautious man, I though I would start with a smallest, smallest quantity. Namely I started with 0.25mg.....and my intention was to increase dosage to see if something will happened. That very small dosage, the first dose of my experiments i planed, was very very strong.
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Hofmann1943
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I would say mic is beating Aspergillus Flavus. It looks like mic is stronger but the final battle is yet to come.
-------------------- By Albert Hofmann : Been cautious man, I though I would start with a smallest, smallest quantity. Namely I started with 0.25mg.....and my intention was to increase dosage to see if something will happened. That very small dosage, the first dose of my experiments i planed, was very very strong.
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total
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Re: A+,B+ VS Trichoderma [Re: pepper]
#13711966 - 12/31/10 03:26 PM (13 years, 5 months ago) |
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Quote:
pepper said: If I owned those Trich dishes I would be scared to open them. How are you going to isolate the good mycelium with out putting billions of Trich in the area that you are working in?
Just a heads up pepper... There will come a time when you REALLY want to save the mycelium on a dish... If you're really scared to open them, make a few transfers inside clear trash bags... That should have been done long before that plate turned green and sporulated...
Trich Mycelium = WHITE Trich Spores = GREEN
Dont be afraid to practice cleaning up dirty plates!
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Hofmann1943
explorer



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Re: A+,B+ VS Trichoderma [Re: total]
#13748619 - 01/07/11 04:46 PM (13 years, 5 months ago) |
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Here it is grand finale.
It don't look like its eating mold, but rather go over it and suffocate it.
Next step is clean up the dishes. Cut from all 3 dishes the cleanest part and put them on the next agar plates. When put the sliced piece on next plate i place micelium directly on agar not agar on agar. So mycelium dont have to get down from the sliced piece as it's already touching new agar, this sould give the myc advantage. Will post again on this.
mushrooms must prevail.
-------------------- By Albert Hofmann : Been cautious man, I though I would start with a smallest, smallest quantity. Namely I started with 0.25mg.....and my intention was to increase dosage to see if something will happened. That very small dosage, the first dose of my experiments i planed, was very very strong.
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roaddog
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wow, good luck. Thats got alot of contams. How many transfers do you anticipate, before you have clean dishes.
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Hofmann1943
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Re: A+,B+ VS Trichoderma [Re: roaddog]
#13748661 - 01/07/11 04:53 PM (13 years, 5 months ago) |
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Thanks I hope 2.
-------------------- By Albert Hofmann : Been cautious man, I though I would start with a smallest, smallest quantity. Namely I started with 0.25mg.....and my intention was to increase dosage to see if something will happened. That very small dosage, the first dose of my experiments i planed, was very very strong.
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roaddog
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So your planning on cutting from the center, where the myk looks more healthy? I dont think the mold is being necessarily suffocated. Im afraid If you try to cut your next transfer wedge, from the sections where the myk, has covered the mold, you will simply transfer the mold along with the myk. I think I would cut small pieces from the center and try to get enough to do several dishes, so you can up your odds. I have never been successful at what you are doing, So please keep us updated. cant wait to see what happens.
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Hofmann1943
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Re: A+,B+ VS Trichoderma [Re: roaddog]
#13748957 - 01/07/11 05:48 PM (13 years, 5 months ago) |
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Here is the places i cut from.
-------------------- By Albert Hofmann : Been cautious man, I though I would start with a smallest, smallest quantity. Namely I started with 0.25mg.....and my intention was to increase dosage to see if something will happened. That very small dosage, the first dose of my experiments i planed, was very very strong.
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Hofmann1943
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I dont want to spend to much resources on this experiment, so i wont transfer to more than one dish. If one of this cubes were important to me i would do that probably. Sure will keep you updated.
-------------------- By Albert Hofmann : Been cautious man, I though I would start with a smallest, smallest quantity. Namely I started with 0.25mg.....and my intention was to increase dosage to see if something will happened. That very small dosage, the first dose of my experiments i planed, was very very strong.
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pepper
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Quote:
Hofmann1943 said: I dont want to spend to much resources on this experiment, so i wont transfer to more than one dish. If one of this cubes were important to me i would do that probably. Sure will keep you updated.
Well this has been a good thread for me because of what your are doing under the conditions that you are doing it. Thanks for following threw to the end.
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Hofmann1943
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Re: A+,B+ VS Trichoderma [Re: pepper]
#13749312 - 01/07/11 06:55 PM (13 years, 5 months ago) |
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your welcome pepper
-------------------- By Albert Hofmann : Been cautious man, I though I would start with a smallest, smallest quantity. Namely I started with 0.25mg.....and my intention was to increase dosage to see if something will happened. That very small dosage, the first dose of my experiments i planed, was very very strong.
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