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FrankS
Stranger
Registered: 02/21/03
Posts: 25
Last seen: 19 years, 4 months
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First time Grower - Seeing green stuff after 4 days
#1367962 - 03/12/03 03:40 AM (21 years, 22 days ago) |
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Hi people
I inoculated my PF-TEK jar on 8 march... But when i look now, i see strange green things in it.. I have no clue what this is?
http://members.home.nl/zniper/paddo.jpg
there's a picture, please help me!!
-------------------- Do what you want, but watch your HP
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PooGrower
Mr. Hanky
Registered: 11/01/02
Posts: 542
Loc: East Side
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Re: First time Grower - Seeing green stuff after 4 days [Re: FrankS]
#1367975 - 03/12/03 03:45 AM (21 years, 22 days ago) |
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Thats definately contamination. That jar is no good theres to much of it to even think about cutting it out. So you need to work on sterilization becaues your not doing a step properly or it wouldnt turn green. Did you sterilize it at 208 degrees in a pot with water? if you didnt go above 200 basically your probably gonna have contams. My first attempt was done wrong because i didnt have it hot enough. I suggest you go buy a turkey thermometer if you dont hvae one.
-------------------- "I WONT STOP TILL I HAVE A LIFE TIME SUPPLY" "dont be offended by anything i say, its in a joking manner"
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FrankS
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Re: First time Grower - Seeing green stuff after 4 days [Re: PooGrower]
#1367987 - 03/12/03 03:49 AM (21 years, 22 days ago) |
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hm... i dont know how hot it was... but it steamed damn well and I coul'nd touch the jar when it came out :/
I have no idea how to get it 200+ degrees
my steamer can reach up to 120 Celcius
-------------------- Do what you want, but watch your HP
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psilosinner
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Re: First time Grower - Seeing green stuff after 4 days [Re: FrankS]
#1368062 - 03/12/03 04:35 AM (21 years, 22 days ago) |
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Franks - 120 degrees Celsius is 248 degress Fahrenheit so that is really close since 15Psi is 250 degrees.
The contamination could have came during the innoculation process and not necessarily during the sterilization process.
You might want to look at the way you are innoculation the substrate.
-------------------- "I don't like people who take drugs... Customs men for example." - Mick Miller -
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djd586
Underpants Gnome
Registered: 02/03/03
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Re: First time Grower - Seeing green stuff after 4 days [Re: FrankS]
#1368074 - 03/12/03 04:41 AM (21 years, 22 days ago) |
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Throw it out and start over. I'm sure you'll take an extra level of precaution and sterilization next time around. But don't get too down, even the most experienced of growers experience contams from time to time.
-------------------- Phase 1... collect underpants... phase 2...??? ... Phase 3 - PROFIT!
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FrankS
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Last seen: 19 years, 4 months
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Re: First time Grower - Seeing green stuff after 4 days [Re: psilosinner]
#1368077 - 03/12/03 04:43 AM (21 years, 22 days ago) |
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haha ok sorry, little mis understanding there - I live in The Netherlands you see
Yes I already did'nd have a good feeling about the inoculation process...
I only heated the tip on the syringe, not the whole needle... but i shove it in completley... maybe that's the problem?
-------------------- Do what you want, but watch your HP
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FrankS
Stranger
Registered: 02/21/03
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Last seen: 19 years, 4 months
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Re: First time Grower - Seeing green stuff after 4 days [Re: djd586]
#1368083 - 03/12/03 04:46 AM (21 years, 22 days ago) |
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Quote:
Throw it out and start over. I'm sure you'll take an extra level of precaution and sterilization next time around. But don't get too down, even the most experienced of growers experience contams from time to time.
thanks for giving me more motivation to get going I was soo happy that every thing worked out right, at least i thought it did :|
Until my mom said to me, hey Frank.. there are some green things in the jar, is that ok?
Oh well, omw preparing some new mixture :D hihi
-------------------- Do what you want, but watch your HP
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Anonymous
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Re: First time Grower - Seeing green stuff after 4 days [Re: FrankS]
#1368100 - 03/12/03 04:54 AM (21 years, 22 days ago) |
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Take your pic out from under your name please
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PJDIDDLE
Registered: 10/17/02
Posts: 2,837
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*DELETED* [Re: FrankS]
#1368101 - 03/12/03 04:54 AM (21 years, 22 days ago) |
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Post deleted by ScriptReason for deletion: .
Edited by PJDIDDLE (06/08/10 05:30 PM)
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FrankS
Stranger
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Re: First time Grower - Seeing green stuff after 4 days [Re: fee]
#1368111 - 03/12/03 04:58 AM (21 years, 22 days ago) |
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Quote:
Take your pic out from under your name please
Ehm... sorry I don't quite understand?
hm... I dont really have 98% alcohol in my house
can I dehydrate some 50% Whisky?
-------------------- Do what you want, but watch your HP
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psilosinner
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Re: First time Grower - Seeing green stuff after 4 days [Re: FrankS]
#1368120 - 03/12/03 05:02 AM (21 years, 22 days ago) |
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You don't have to use 98% alcohol (i.e EVERCLEAR) just use rubbing alcohol
-------------------- "I don't like people who take drugs... Customs men for example." - Mick Miller -
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psilosinner
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Re: First time Grower - Seeing green stuff after 4 days [Re: PJDIDDLE]
#1368125 - 03/12/03 05:04 AM (21 years, 22 days ago) |
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Script - flaming the needle after each jar is real time consuming. Use an alcohol soaked paper towel or a cotton ball to clean the needle every time you remove it from the jar.
-------------------- "I don't like people who take drugs... Customs men for example." - Mick Miller -
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PJDIDDLE
Registered: 10/17/02
Posts: 2,837
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Post deleted by ScriptReason for deletion: .
Edited by PJDIDDLE (06/08/10 05:30 PM)
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psilosinner
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Re: First time Grower - Seeing green stuff after 4 days [Re: PJDIDDLE]
#1368161 - 03/12/03 05:21 AM (21 years, 22 days ago) |
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So true
-------------------- "I don't like people who take drugs... Customs men for example." - Mick Miller -
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Anno
Experimenter
Registered: 06/17/99
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Re: First time Grower - Seeing green stuff after 4 days [Re: FrankS]
#1368168 - 03/12/03 05:24 AM (21 years, 22 days ago) |
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If you sterilized the jars in the pressure cooker(how long? ) then the mold contamination is likely not due to insuficient sterilisation. Where do you see the mold, is it on the inoculation points or throughout the jar? Edit: Ok, I missed the picture first..... Looks like your spore suspension is contaminated. Where did you get the spore syringe from?
Edited by Anno (03/12/03 05:26 AM)
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FrankS
Stranger
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Last seen: 19 years, 4 months
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Re: First time Grower - Seeing green stuff after 4 days [Re: Anno]
#1368205 - 03/12/03 05:38 AM (21 years, 22 days ago) |
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hm.... got the syringe from a guy I know from the internet, He does'nt seem like a person to make a contaminated syringe at all though... oh well, I just give it another try -edit- on and btw... i used the PF-TEK method as in your Signature - PF-TEK for simple minds
-------------------- Do what you want, but watch your HP
Edited by FrankS (03/12/03 05:40 AM)
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Anno
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Re: First time Grower - Seeing green stuff after 4 days [Re: FrankS]
#1368310 - 03/12/03 06:15 AM (21 years, 22 days ago) |
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As a test, when you make your next batch, leave one jar non inoculated and see what happens with it. If it doesn?t contaminate, you know that your sterilization procedure before inoculation is OK.
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