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OfflineMoronacracy
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CHopped Mycelium cakes for inoc?
    #1367000 - 03/11/03 07:34 PM (14 years, 9 months ago)

Sorry if posted elsewhere.

Anyone had success with: putting a pf cake in a blender with water and chopping, then sucking up mycelium with a syringe for inocluation? Wouldn't this be similar to mycelium liquid?


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OfflineAuradefect
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Re: CHopped Mycelium cakes for inoc? [Re: Moronacracy]
    #1367010 - 03/11/03 07:36 PM (14 years, 9 months ago)

to me it sounds like it might work, i dont see why it wouldnt.
i am no expert and have never tried this myself, id wait for someone who knows more to respond.


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OfflinePhychotron
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Re: CHopped Mycelium cakes for inoc? [Re: Auradefect]
    #1367393 - 03/11/03 09:58 PM (14 years, 9 months ago)

thier's always one way to find out


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Invisiblegeorge castanzaM
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Re: CHopped Mycelium cakes for inoc? [Re: Moronacracy]
    #1367406 - 03/11/03 10:19 PM (14 years, 9 months ago)

if you keep it STERILE it will work.STERILE  :shocked:


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OfflineSemperUbiSubUbi
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Re: CHopped Mycelium cakes for inoc? [Re: george castanza]
    #1367460 - 03/11/03 11:31 PM (14 years, 9 months ago)

george is right sterility is key.
Here is a method not using a blender (just made up)
get a jar
break it (JUST DO IT!)
get another jar
put a chunk or 2 of broken glass in your 2nd jar
put some water in there
poke a hole in the lid
put lid on and pressure cook your jar of glass chunk soup
wait for it to cool
(now this is the hard part)
using sterile techniques drop a clean piece of mycelium/inner mushroom tissue into your sterile glass chunk soup
shake thoroughly
suck it up with a sterile syringe and use normally. (you should see growth within a day or 3).
your welcome  :smile:


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OfflineSemperUbiSubUbi
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Re: CHopped Mycelium cakes for inoc? [Re: SemperUbiSubUbi]
    #1367476 - 03/12/03 12:01 AM (14 years, 9 months ago)

After thinking about this awhile longer, I have come to the conclusion that you could use hydrogen peroxide in place of water and skip the sterilization process altogether. OR --even better--
The ultimate way would probably be a combo of pressure cooking and hydrogen peroxide. such as when you have your internal shroom tissue on your scalpel and are about to plop it in your "glass chunk soup" dip it in a bath of (3% out of the bottle) h202 and let it fry for a minute or two then drop it in. yeah that sounds good...Help me out on this one guys, What do you think?
Round up the FOAF's and tell em to get to work.


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OfflinePantherina
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Re: CHopped Mycelium cakes for inoc? [Re: SemperUbiSubUbi]
    #1367500 - 03/12/03 12:34 AM (14 years, 9 months ago)

You could dunk it in H2O2 but u are better propably better droping some of the colonised caka from the center into sterlised H2O with some gravel in and using these to mash up the cake. I have used this method successfully just working in a glove box. Using a blender just adds an extra area of contamination, so use the sealed jar technique. If inoculating cakes, growth will start much quicker than starting from spores. I have also used this tech to inoculate straw and grain successfully.


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