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Offlineethnofun
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Re: using alreary colonized sub. to colonize more [Re: ethnofun]
    #13669361 - 12/22/10 03:24 AM (13 years, 4 months ago)

Also just to clear it up. It was taking fully colonized coir about to be on its 3 rd flush here soon finishong up second here soon, and putting chunks into new coir. Then putting that into colonize. No need to i have 10 quarts of wbs colonizing as we speak. Again just a question i thought would be good to know.

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Invisibleanonjon
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Re: using alreary colonized sub. to colonize more [Re: enlightenment]
    #13669533 - 12/22/10 07:59 AM (13 years, 4 months ago)

Quote:

enlightenment said:
Quote:

anonjon said:
No. Old substrates are not vigorous and not resistant to contamination. You wouldn't want to use them as an inoculant.




I disagree. You can transfer colonized substrate to a petri dish.
You can use peroxide agar. Use the search.





Inexperienced noob telling me to use the search engine so I can learn to use old substrate blocks to inoculate agar.

:tardpig:

No thanks. I'll use a fresh young pin to clone instead of some dirty, depleted material.

Use the search engine. :wow:  :nono:  :n00bslayer:


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The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:

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Offlinebroken
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Re: using alreary colonized sub. to colonize more [Re: ethnofun]
    #13669563 - 12/22/10 08:09 AM (13 years, 4 months ago)

Quote:

ethnofun said:
Also just to clear it up. It was taking fully colonized coir about to be on its 3 rd flush here soon finishong up second here soon, and putting chunks into new coir. Then putting that into colonize. No need to i have 10 quarts of wbs colonizing as we speak. Again just a question i thought would be good to know.




Once something has fruited just get as many flushes as you can out of it.  :whathesaid: this would not work.


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:willynilly:

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Offlineeniac
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Re: using alreary colonized sub. to colonize more [Re: broken]
    #13669569 - 12/22/10 08:13 AM (13 years, 4 months ago)

There's a bit about this in The Mushroom Cultivator.
I guess people used to do it, but nobody has in the last fifty years because it doesn't work well.

Super spawning? Active spawning? Something like that. At any rate, don't bother.

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Offlineethnofun
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Re: using alreary colonized sub. to colonize more [Re: eniac]
    #13673886 - 12/23/10 12:02 AM (13 years, 4 months ago)

Thats crazy i need to read that book been meaning to buy it. Ill have look in to it more. Might try if I end up with extra coir next tubs

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OfflineThe shroomy 1
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Re: using alreary colonized sub. to colonize more [Re: enlightenment]
    #13674122 - 12/23/10 01:31 AM (13 years, 4 months ago)

Quote:

enlightenment said:
Quote:

closed veil said:
Quote:

The shroomy 1 said:
Quote:

enlightenment said:
IMO agar is also a substrate. :laugh:




How so?  Unless you are cultivating "fruiting bodies" on agar!  If you're doing that, then I invite you to join the rest of of that are getting "Real" Harvests.  What kind of harvests are you getting on agar?  I WANT TO SEE PICTURES OF THIS! :rofl2:




i agree this is funny. but stranger things have happened. i had a LC once make i tiny little fruit.




For me a substrate is a surface mycelium lives upon.
That doesnt mean that i fruit from agar -.-

Maybe he has only this one cake and no spores left.
So he could transfer a piece of his cake to agar.
From one petri dish he can save this culture and inoculate a lot of new jars. Or he can make a LC from the agar and innoc. 100000+ jars.

Are you pleased now? :rolleyes:



Does it really matter?      I didn't think so!
You said the magic word...."For You". 
Please understand that "LANGUAGE" is a very POWERFUL tool in any community.  We have to be "CLEAR" on what a word means or it can be misused and diluted down to the point that the word will end up meaning something else.  All I'm asking is that you be responsible in how you give advice here in this community.  Nothing more nothing less. :mushroom2:


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AMU Q&A thread.

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OfflineRogerRabbitM
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Re: using alreary colonized sub. to colonize more [Re: ethnofun]
    #13674540 - 12/23/10 06:08 AM (13 years, 4 months ago)

Quote:

ethnofun said:
Also just to clear it up. It was taking fully colonized coir about to be on its 3 rd flush here soon finishong up second here soon, and putting chunks into new coir. Then putting that into colonize. No need to i have 10 quarts of wbs colonizing as we speak. Again just a question i thought would be good to know.




This will not succeed.

One reason is that by the time it's flushed, the substrate is covered in contaminant spores, some of which have already germinated and begun to grow.  If you spawn this to freshly pasteurized substrate, the molds will outgrow the mushroom mycelium.

The other reason is that the mycelium is old and tired.  After a few flushes, they don't stop producing due to running out of nutrients, but rather because the mycelium has produced fruits and reached the end of it's natural life cycle.  Giving this old mycelium a fresh substrate would be like giving an old man a steak dinner.  It's not going to make him young and virile again.
RR


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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Invisibleblackdust
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Re: using alreary colonized sub. to colonize more [Re: ethnofun]
    #21021929 - 12/24/14 03:36 PM (9 years, 4 months ago)

Just subscribing to the thread. Do not write me or quote my message. If you feel the need to talk to me then PM me or I will PM you. I also hang out in jounals. I refuse to post on the board. I am just subscribing to this thread.

:rooster:

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Invisibleeatyualive
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Re: using alreary colonized sub. to colonize more [Re: blackdust]
    #21022481 - 12/24/14 07:02 PM (9 years, 4 months ago)

its called "super spawning".


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EAT GETS SHIT DONE


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Invisibleelasticaltiger
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Re: using alreary colonized sub. to colonize more [Re: eatyualive]
    #21022547 - 12/24/14 07:23 PM (9 years, 4 months ago)

This is called super spawning and from what I know it's unreliable at best.

Search Super Spawning to see how it turned out for others.

If I was going to super spawn I would do it out doors and with freshly colonized sub.

But that's just like... my opinion man.


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OfflineYakul
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Re: using alreary colonized sub. to colonize more [Re: The shroomy 1]
    #21063845 - 01/03/15 10:03 PM (9 years, 4 months ago)

Great thread.  Cleared up lots of misunderstandings I had.  Thank you's to Shroomy1 and RR and those who posed the questions.


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"Only an idiot is entirely certain about everything all the time."-Sadguru

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