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Offlineslim26
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Junk it or give it more time
    #13625274 - 12/13/10 08:09 AM (13 years, 3 months ago)

I got what seems to be contam in 1 out of 12 jars.  This is after 7 days. I separated the jar from the others.  Should I let it play out a little more or junk it to be safe?


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OfflineRogerRabbitM
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Re: Junk it or give it more time [Re: slim26]
    #13627633 - 12/13/10 05:31 PM (13 years, 3 months ago)

It doesn't need more time.  Toss it. Boil the jar for an hour or two before opening to clean. The location of the trichoderma adjacent to the mushroom mycelium indicates contaminated inoculant or failure to flame sterilize your needle.  Either way, the trich entered at inoculation.
RR


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Offlineslim26
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Re: Junk it or give it more time [Re: RogerRabbit]
    #13628754 - 12/13/10 09:03 PM (13 years, 3 months ago)

Thanks... so far it looks like I still have 11 of 12 that are good... so probably my neglect to flame sterilize on this one.

I boil the jar with the contaminated substrate in it?

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Offlinepepper
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Re: Junk it or give it more time [Re: slim26]
    #13629152 - 12/13/10 10:24 PM (13 years, 3 months ago)

Yes, you do not want to let the trichoderma spores out.
When you boil the jar you kill the contaminants in the jar.

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