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InvisibleEvilMushroom666
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Pl. djamor Agar Clone Experiment
    #13534555 - 11/24/10 10:15 AM (13 years, 3 months ago)

So about 7 weeks ago I took a MS swipe from a Pl. djamor print and put
it to agar. I have heard people say that it can be difficult to find
a good fruiting culture from some edibles when using spores so here is
a little experiment that is underway:

7 weeks ago MS swipe to agar

Nov 24th 2010-
Few small clusters of Pl. djamor fruiting from agar.
Selected the best looking cluster and transferred to another dish.



From here I plan to let the clone grow out and see if it sectors,
and if so make transfers from each sector. This is more of a fun
little experiment then anything, and I plan to slant up the most
aggressive isolate I can get from this cluster and at a later time
play with fruiting it. Anyone bored and looking to play with pinks?
:bubbles:


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Offlinetotal
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Re: Pl. djamor Agar Clone Experiment [Re: EvilMushroom666]
    #13536452 - 11/24/10 05:03 PM (13 years, 3 months ago)

:popcorn:


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Offlinenexus1946
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Re: Pl. djamor Agar Clone Experiment [Re: total]
    #13537776 - 11/24/10 10:14 PM (13 years, 3 months ago)

Looks good!


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InvisibleEvilMushroom666
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Re: Pl. djamor Agar Clone Experiment [Re: nexus1946]
    #13562289 - 11/30/10 10:56 AM (13 years, 2 months ago)

Here is a picture of the plate on Nov 27th. Mycelium is starting to grow
from the clone tissue. Some of the pins pictured on top actually
dropped spores onto the agar which then germinated in a pattern
following the flow of water droplets away from the spores.
Nov 27th


Nov 30th


As you can see the trail of spores is germinating on the plate with
no visual contaminates as of yet. As well I took two transfers from
the emerging mycelium from the pins to see if they sector out. This
has turned into a Frankenstein like experiment lol.


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InvisibleEvilMushroom666
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Re: Pl. djamor Agar Clone Experiment [Re: EvilMushroom666]
    #13597017 - 12/07/10 04:55 PM (13 years, 2 months ago)

Dec 7th:
Here is a picture of the clone dish grown out a bit. As you can see I
took 3 small wedges from the original dish and here is the sectoring
that has occurred.


I took 6 sectors(Could have done a few more but choose not to) and
transferred them further to see if I have reached a few monocultures.


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Offlinedeadmandave
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Re: Pl. djamor Agar Clone Experiment [Re: EvilMushroom666]
    #13597326 - 12/07/10 05:51 PM (13 years, 2 months ago)

awesome, is it common to get fruits on a colonized agar plate?


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InvisibleEvilMushroom666
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Re: Pl. djamor Agar Clone Experiment [Re: deadmandave]
    #13597359 - 12/07/10 05:57 PM (13 years, 2 months ago)

I do not remember were I read it, and I may be wrong but I have heard
that if it fruits from agar chances are it is an aggressive and
vigorous specimen. So far I have seen Oyster Mushrooms and Lions Mane
fruit directly from agar.


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