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Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag

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OfflineCreeper
Stranger
Registered: 03/06/03
Posts: 4
Last seen: 20 years, 9 months
Slowed Mycelium Growth
    #1354000 - 03/06/03 05:05 AM (21 years, 18 days ago)

I'm a first timer though i've helped friends before with projects i know very little. We used standard rice/vermiculite pf tek with quantities doubled for pint jars. For the first week everything was fine with roughly 40% of the jars colonized after 6 days. Now after 20 days the growth has slowed and only 55% of the jars colonized. There are no visible contaminents but he beginning to worry, there is still growth just very slowly. Should he rehydrate the jars or wait it out? If he waits how much longer could this take and if hydration is necessary how much? Thanks ahead for any help.

Edited by Creeper (03/06/03 05:33 AM)

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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: Slowed Mycelium Growth [Re: Creeper]
    #1354005 - 03/06/03 05:09 AM (21 years, 18 days ago)

Lack of air exchange can cause jars to stall. Are they able to breathe?


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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.

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OfflineCreeper
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Registered: 03/06/03
Posts: 4
Last seen: 20 years, 9 months
Re: Slowed Mycelium Growth [Re: dog]
    #1354033 - 03/06/03 05:22 AM (21 years, 18 days ago)

Not really sure what ya mean, like i said i'm a first timer. But the lids are on tight with the holes on the cap retaped. What would you recommend i do to let them breathe?

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Offlinethe spiral
Neuroscientist
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Folding@home Statistics
Registered: 05/13/02
Posts: 1,769
Last seen: 8 years, 7 months
Re: Slowed Mycelium Growth [Re: Creeper]
    #1354047 - 03/06/03 05:28 AM (21 years, 18 days ago)

turn them upside down and take the tape off of the holes.
this lets carbon dioxide to drain out and fresh air to come in.


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"A celibate clergy is an especially good idea, because it tends to suppress any hereditary propensity toward fanaticism." - Carl Sagan

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InvisibleChe_Night_Soil
Koinoniphobic

Registered: 07/16/02
Posts: 1,533
Loc: Commonslaughtia
Re: Slowed Mycelium Growth [Re: Creeper]
    #1354049 - 03/06/03 05:29 AM (21 years, 18 days ago)

If you feel your incubator is a clean place :smirk:, you can remove the tape. Do your jars have verm layers on top? 


--------------------
if(human==autoPart){
for(i=1;i<infinity;i++){
getBorn(mind,soul);
getEdu(mind,soul);
getJob(mind,soul);
retire(mind,soul);
die(mind,soul);
}
}

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OfflineCreeper
Stranger
Registered: 03/06/03
Posts: 4
Last seen: 20 years, 9 months
Re: Slowed Mycelium Growth [Re: the spiral]
    #1354056 - 03/06/03 05:32 AM (21 years, 18 days ago)

Thanks, i'll try that, how long should he let them air out?
Yes there is a dry verm layer on top.

Edited by Creeper (03/06/03 05:35 AM)

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Offlinethe spiral
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Re: Slowed Mycelium Growth [Re: Creeper]
    #1354063 - 03/06/03 05:36 AM (21 years, 18 days ago)

keep them upside down overnight, that should be enough.


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"A celibate clergy is an especially good idea, because it tends to suppress any hereditary propensity toward fanaticism." - Carl Sagan

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InvisibleChe_Night_Soil
Koinoniphobic

Registered: 07/16/02
Posts: 1,533
Loc: Commonslaughtia
Re: Slowed Mycelium Growth [Re: Creeper]
    #1354075 - 03/06/03 05:40 AM (21 years, 18 days ago)

How big are your holes? If you made them with a nail, or whatever, just remove the tape from two of the holes and leave it off. Make sure the inside of your incubator is a sterile environment, though. Get a second opinion too. Actually, sorry, listen to harry manback. If your stuff is a little dry leaving the tape off could be bad. Flipping displaces Co2 immediately, so you can set them upright and put the tape back on after doing so.


--------------------
if(human==autoPart){
for(i=1;i<infinity;i++){
getBorn(mind,soul);
getEdu(mind,soul);
getJob(mind,soul);
retire(mind,soul);
die(mind,soul);
}
}

Edited by Che_Night_Soil (03/06/03 06:01 AM)

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OfflineCreeper
Stranger
Registered: 03/06/03
Posts: 4
Last seen: 20 years, 9 months
Re: Slowed Mycelium Growth [Re: Che_Night_Soil]
    #1354102 - 03/06/03 05:50 AM (21 years, 18 days ago)

used a knife and made the holes just small enough for the syringe to pass through. would any of this cause the jars to lose moisture?

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InvisibleChe_Night_Soil
Koinoniphobic

Registered: 07/16/02
Posts: 1,533
Loc: Commonslaughtia
Re: Slowed Mycelium Growth [Re: Creeper]
    #1354113 - 03/06/03 05:55 AM (21 years, 18 days ago)

^ edit.


--------------------
if(human==autoPart){
for(i=1;i<infinity;i++){
getBorn(mind,soul);
getEdu(mind,soul);
getJob(mind,soul);
retire(mind,soul);
die(mind,soul);
}
}

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