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iwanna
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Registered: 10/08/10
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Spawn Bag Help
#13526451 - 11/22/10 08:03 PM (14 years, 1 month ago) |
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Hi all,
I just received (2) 3lb pre-sterilized spawn bags (rye berries) from everything mushrooms today. I got the pre-sterilized ones because I haven't had the money to get a PC yet.
I had a couple questions... First is, I would like to inoculate the bags with a multispore syringe. If I do this how many cc's of solution should I use? I think I have read 3 to 5 as you don't want to mess up the water content of the bag, however I want to confirm.
Second question is, these bags are sealed at the top and are pretty air tight all the way up to the air exchange port up on the top. Do I need to cut them open (In a glovebox) and reseal them with wire in order to allow for air exchange? I would think yes but wanted to confirm before cutting open the bag and risking contams.
Finally... Is there are better (or faster) way to knock these bags up. I have about 40 PF cakes that are in either 100% colonized or very close to that. Could I just throw a nice chunk of mycelium in there?
Thanks for your help.
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iwanna
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Registered: 10/08/10
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Re: Spawn Bag Help [Re: iwanna]
#13528308 - 11/23/10 06:34 AM (14 years, 1 month ago) |
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Hey guys...
So I have been reading more and more... plus I have RRs video, but I just cant get the low down on these spawn bags.
Last night i read a post and jumped the gun inoculating them with 2.5cc (each) of multispore solution. After reading more, i think this may have been a bad choice.
Most everyone here is using LC to knock up their bags... however i have read RR saying LC is a bad choice as you cant tell if its contam'd until after it colonizes the substrate.
I'd really like to get the best out of these bags, should I just hit them up with more spore solution? I have 2.5 syringes left for now. I could use mycelium water from a PF cake?
Any advice?
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Hamurabi
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Re: Spawn Bag Help [Re: iwanna]
#13528316 - 11/23/10 06:39 AM (14 years, 1 month ago) |
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iwanna please check your PMs.
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hamloaf
Q-dood ®©™√



Registered: 12/23/09
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Re: Spawn Bag Help [Re: iwanna]
#13528330 - 11/23/10 06:52 AM (14 years, 1 month ago) |
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Hello, there. Welcome to the Shroomery! 
First off, how much did you pay for your pre-sterilized bags with shipping? I would almost guarantee you, you could'v poked around ebay and found a you pressure cooker for the same amount of money you spent on the pre-sterilized bags with shipping.
Your post is dirty, before even considering how many cc's to inoculate the bag up with, let alone a better or faster way to inoculate spawn bags, we must first solve the problem of your bags being air tight and with out an air plenum.
Spawn bags being air tight is not the correct shape of spawn bag for inoculation. The spawn bags are going to need a plenum of air in order for the mycelium to grow properly. The reason your bags came air tight is so the vendor can fit more bags in the sterilizer at once and for the purposes spacial orientation during shipping.
I remove the air out my spawn bags before sterilization as well so that I can fit more bags in the sterilizer at once. After the bags are cooled to room temperatures they will be in an air tight vacuum seal. The thing you don't want to do is cut them open in a glove or still air box, give them an air plenum, and then seal the bag again. Forget about that. You could get away with that with a flowhood because the air you would be filling the spawn bags with would be sterile. Other wise you will be inviting every contaminate in your house into you spawn bags.
What works for me is to place them on a shelf at room temperatures separating the air tight seal of the bags with my hands and kind of pulling the opposing sides apart from each other until the bag no longer sticks together. The whole mean while being mindful not to touch the filter patch as well as not to let any grains touch the filter patch. A few grains are going to touch the patch and that can't be helped. Just don't let grains that stick to the filter patch remain stuck to the filter patch.
Now this is where patience comes into play. Making spawn bags with an air tight seal takes about 5 days, repeating the process of pulling the opposing sides apart from each other at least twice a day, at room temperatures before there's an ample air plenum to support mycelial growth.
After a couple of days of this process the bags will begin to inflate with air. When the bags have begun to inflate the grains will begin to be able to be moved and shook around freely. When enough air has inflated your bags (which will be by about day 2 or 3) begin rolling the grains around in the bags when you go in to pull the opposing sides away from each other. Again, make sure no grains remain stuck to the filter patch between handlings.
By the fifth day, if you have handled the bags as I have described, your bags will have an ample air plenum to support mycelial growth and will be ready to be inoculated.
Quote:
I would like to inoculate the bags with a multispore syringe. If I do this how many cc's of solution should I use? I think I have read 3 to 5 as you don't want to mess up the water content of the bag, however I want to confirm.
When inoculating spawn bags with spores, prepare to be patient. Spores could take up to 2 weeks to germinate. When I used spore syringes and spawn bags, I would inoculate the bags at four separate inoculation points between the bag and substrate so you can see germination using 1 cc each inoculation point. Worked for me.
Quote:
Is there are better (or faster) way to knock these bags up. I have about 40 PF cakes that are in either 100% colonized or very close to that. Could I just throw a nice chunk of mycelium in there?
There's really no "faster" way to inoculate spawn bags. It's more a matter of which inoculant you use that determines the speed of colonization once said inoculant is introduced to the substrate. Inoculating spawn bags with liquid culture, a live agar culture, or grain to grain transfer are all superior ways to inoculate spawn bags. Don't brown rice flour to brown rice flour or grain transfer. I haven't read good things about this and would not personally do it due to the amount of contaminates fully colonized brown rice flour could harbor. Taking a chunk of colonized brown rice flour cake to inoculate a spawn bag seems illogical to me.
Fruit all those cakes as cakes inside of a shotgun terrarium. If you really want to play, fruit half your cakes as cakes and crumble the other half of the cakes and spawn the crumbled cakes to a bulk substrate in trays and fruit the trays inside of a shotgun terrarium as well. The variation between the several methods of how you can fruit brown rice flour cakes is really at your own discretion.
Edited by liquidmyce (11/23/10 08:40 AM)
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iwanna
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Re: Spawn Bag Help [Re: hamloaf]
#13529916 - 11/23/10 01:39 PM (14 years, 1 month ago) |
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Thanks so much for the reply hamloaf I really appreciate it.
First if.. you are correct, I would have been better off just getting a PC off ebay. However i really don't want to cheap out on my PC I am planning on getting an american standard for $200, and at the time I only had like 40 bucks to burn... So I picked up the bags. I knew on the front end I was throwing money out the window. I couldn't get visions of bulk out of my head.
Ok... I am going to go ahead and start getting some air into the bags via your outlined approach. Do you think i completely screwed up the bags by all ready inoculating them with 2.5cc each?
Additionally... What would be my best go forward approach? I mean I could pull together an LC and knock them up with that. But it will take some time to do an LC. I could hit them with some more spore solution... or I could leave them as is. Whatever you think will work best.
I am defiantly going to use about 25 of the cakes to spawn to bulk I have a huge bin of straw and another huge bin of H-poo. So fingers crossed I will be good there.
Thanks for taking the time to let me know the deal.
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iwanna
Stranger


Registered: 10/08/10
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Re: Spawn Bag Help [Re: iwanna]
#13543232 - 11/26/10 08:56 AM (14 years, 1 month ago) |
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Bump...
OK so my spawn bags now have a nice pocket of air...
So like I had said. I knocked these things up with 2.5 cc's each of spore solution. Am I ok? Should I add more, or should I make up an LC and try to hit them up with that?
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JerrysBoy
Alchemist


Registered: 12/26/17
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Re: Spawn Bag Help [Re: iwanna]
#24870473 - 12/26/17 09:25 PM (7 years, 22 days ago) |
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Hey guys, so I have a similar situation. This is my second time growing and I'm using a grow kit from shroom supply. My previous grow was using PF Tek and I had very unsuccessful flushes with only about 2 grams of fresh mushrooms from four jars. I decided to use shroom supply because of the overwhelming support of their grow kits, however, after inoculating each 1LB rye berry myco bag with 2-2.5 cc of B+ spore solution (12/7/17) there has been not a single sign of colonization (it is now 12/26/17). Not a single change within the bags at all. I don't even see any contams. I'm speculating that this could be due to cooler temperatures because I do not use any direct heating to the bags. The bags sit on a towel on the top shelf of my closet in mostly darkish lighting. and keep between 60-75 degrees Fahrenheit. each bag is unfolded and their gas exchange ports are exposed to proper gas exchange. If this is due to cold temperatures what should I do? can I re-inoculate them through the injection ports if I keep them warm? will they colonize if I introduce more warmth at this point since inoculation?
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