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Original Sensible Seeds Shop: High THC Strains

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InvisibleGerman Kahuna
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Re: 1 lbs MEA & 500 petri dishes [Re: hamloaf]
    #13457428 - 11/09/10 03:06 AM (13 years, 3 months ago)

I PC my agar in pint jars and pour it from there. Works a treat. Since I can usually do around 20 dishes before the agar starts to solidify I don't need anything bigger.


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"Vegetarian" [ /ˌvedʒəˈteəriən/] - Ancient slang meaning "village idiot who can't hunt, fish or ride".


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Invisibleandymc
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Re: 1 lbs MEA & 500 petri dishes [Re: German Kahuna]
    #13457524 - 11/09/10 04:16 AM (13 years, 3 months ago)

*sigh*

It's a shame when threads needlessly descend into a pissing contest.  Ham, you're going to start a flame war and start insulting this guy over which brand of whiskey bottle RR uses?

JHC


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Invisiblehamloaf
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Re: 1 lbs MEA & 500 petri dishes [Re: German Kahuna]
    #13457549 - 11/09/10 04:44 AM (13 years, 3 months ago)

Nice.

Since I'm only working on 2 strains at the moment, my need for agar is not that extensive, also, to save time and money, the next time I do agar, I'm gonna go with the whole pouring a thin layer of agar into half pint wide mouth or squat jars.  Fit the jars with plastic lids.  Onto the lids, fit a gas exchange port.  With 2 1/16th inch holes and a synthetic filter disk as the gas exchange filter.  Sterilize the whole thing at once.  Let cool in my steroclave overnight.  Pull them out and inoculate.  Cuts out having to purchase petri dishes.  Cuts out having to pour them as well.  You save money, time, as well as cut back on a few contaminate vectors.  Knocks out several birds with one stone.



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Edited by liquidmyce (11/09/10 04:52 AM)


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Offlinegornyhuy
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Re: 1 lbs MEA & 500 petri dishes [Re: hamloaf]
    #13457824 - 11/09/10 07:48 AM (13 years, 3 months ago)

I used a whiskey jar for a while, but I've actually found it a lot easier to work with a pint jar in a glovebox.  It pours fine, and I haven't had any contams on my unused dishes.  The main advantage is it is so easy to work with, fill, and clean/reuse.


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Original Sensible Seeds Shop: High THC Strains


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