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Invisiblestonesun
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A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) * 93
    #13431948 - 11/03/10 11:58 PM (13 years, 4 months ago)
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Intro:


I've decided to put this guide together because there are numerous old threads and outdated
information floating around regarding the cultivation of the sclerotia producing Psilocybes.
There are however some really great threads too, like the Easier than Cubes! or the Sclerotia FAQ.
Both very good reads, highly recommended:thumbup:
I'll  try to cover the most popular and readily available species:

  • Psilocybe galindoi/ATL#7
         
  • Psilocybe mexicana/A
         
  • Psilocybe tampanensis/Pollock
         
  • Psilocybe mexicana/Jalisco


I have worked mainly with these four stone producers for the last year.
Section Mexicanae is my absolute favorite from the Psilocybe genus.
As you probably already know these species have the ability to produce
sclerotia (stones) invitro and fruit bodies as well.
Most people cultivate them for stones only but hopefully after reading this
thread they'll change their mind and give it a go at fruiting 'em too.
They aren't much harder to fruit than P. cubensis just require a thin casing layer
and some extra TLC.
Let's get started!

Grain/BRF Substrate Preparation (From Best to Least Optimal):

  • Rye Berries
         
  • Rye Grass Seed (RGS)
         
  • Wild Bird Seed (WBS)
         
  • Brown Rice Flour (BRF)


I will not go into details with the BRF, WBS and RGS preparation,
because these three methods are truly inferior to a perfectly prepped rye berries substrate.
Here's a short version of those three.

BRF Preparation:
BRF cakes preparation is the regular formula (1/1/2 - BRF/water/verm)
but instead of 100% water use a 50% coffee/50% water solution.
Steam sterilize for 90 minutes or PC for 60 minutes @15psi.

P. tampanensis sclerotia forming on BRF substrate
   

RGS Preparation (/quart jar):
300ml RGS
150ml coffee/water mix (50/50)
Pinch of gypsum
Put RGS and gypsum in your jar, pour coffee/water on it.
Shake to distribute the liquid evenly and let sit for
two hours. Then PC it for 60 minutes @15psi.

P. galindoi sclerotia on Rye Grass Seed
   

WBS Preparation :
I hate WBS because it's messy and pretty difficult to clean the millet
away from the sclerotia.
Use it however if for some reason rye or RGS is not available.
You can also prepare it at the same way like the rye berries below.

P. tampanensis colonizing on Wild Bird Seed
   

Rye Berries Preparation:
As for the rye berries here's a detailed and pictured preparation method.

This is basically identical to RR's Rye Grain Preparation with the
addition of coffee to the soak water.

I start it by brewing a full pot (2 quarts) of strong coffee.
In a large pot/kettle heat up 2-2.5 gallon of water and add the coffee.
Add a couple of teaspoons of gypsum and stir.

Prepping the coffee/water soak
   

While the water/coffee is heating measure out your rye.
Use a cup of rye for each quart jar.
Rinse the grains a few times to clean them from all the dirt/debris.
When the water runs clear drain it. Whenever the coffee/water is around 160F go
ahead and shut off the stove and dump in the grains.
Stir it around, cover and let it sit for 4-24 hours.
I usually do this overnight, with a soaking time of 8-10 hours.

Rinsing the rye and adding it to the coffee/water
   

Your rye is soaked, now it's time to boil.
Bring it up to a full boil and let it go for 10-15 minutes.
Turn off the stove and strain the grains in to large colanders/strainers.
I like to put a fan on them and move them around a few times while drying.
Make sure that the surface of the kernels are completely dry!

Boiling, straining and drying the Rye Berries
   

Now they're ready to load into jars.
Use your favorite lids with your favorite filter (mine is synthetic filter disc).
Tyvek or polyfill will work too, but nothing beats the SFDs.
Screw on lids and put a layer of aluminum foil on them.

Filling the jars and fit them with lid & foil
   

Now you can load the jars in the pressure cooker.
PC them for at least 90 minutes (I like to go with 120...) @15psi.
As always let the PC completely cool before removing the jars.
Jars are room temperature and the foil is removed.
The grains are a little bit compacted so I like to shake them gently a bit now.
Ready to be inoculated!

Jars before & after sterilization
   

Inoculation Methods:

So you have a spore print or syringe.
If you don't want to complicate things or just simply don't
have the supplies/resources go ahead and inoculate the substrate with
your spore syringe. If you have a print, well you know, just make a spore
syringe...

Spore print and spore syringe of P. mexicana
   

If you are a bit advanced and make LCs for you cubensis, these species will
do great in a DME (Dried Malt Extract) and DEX (Dextrose) LC.
Use your favorite recipe.
My preferred one is 1 tsp Malt Extract and 1/8 cup of Dextrose per quart.
The best and most advanced method however is to start your spores on agar.

P. galindoi mycelium on LME/DEX LC
   

I use premixed MEA media and have never had germination issue.
When you have a nice healthy grow on your plate (not going into isolation...),
you can inoculate your grain substrate or LC with a wedge.

P. galindoi and P. mexicana on agar
 

Rye inoculated with agar wedge
   

Now just set it and forget it!
Are you ready for some stones?!

Very often (especially with spore and agar wedge inoculation) sclerotia will
form before full colonization of the substrate. This is perfectly normal.
If you inoculate with LC, the mycelium usually just rips through the substrate
and stones will form after 100% colonization.
Either way you should see stones soon.

Some "young" jars (less than 30 days)
   

There's a controversy on shaking or not at the 20-30% stage of colonization.
Some say it's OK some say don't do it.
I've done it with success, but failed a few times too.
Therefore I don't shake anymore.

When I want to expand and plan on doing G2G transfers, the best method IME
is to start with LC.
I use 3-4cc per jar and it's always done within a week.
Stones are not forming yet or if so, they are very tiny.
Just as usual, break up your colonized grain by shaking your jar,
and transfer a small amount into new sterilized substrate jars.
However if you have a fair amount of LC, I'd rather go that way
instead of G2G.

P. mexicana/A rye jars around 60 days
   

P. galindoi rye jars around 60 days
   

Harvesting the Stones:

Well it is time to enjoy the first "fruits" of your labor.
When you're at this stage, your jars should be at least 2 months or older.
There's no point to harvest before 2 months and my latest harvest was 6 months.
Some leave it longer, but I always in need of jars/space.
Anyhow...
Get a spoon and sanitize it with alcohol.
Scrape out and separate the substrate and sclerotia on aluminum foil.
I have done this before in laminar air flow and totally open air.
Never ever get a contam with either method.

Stones harvested from RGS and rye berries substrate
   

Clean your stones with a brush and store them fresh in paper bags in the fridge
for weeks or dehydrate and store them for months.

P. mexicana/A sclerotia harvested from rye
   

P. galindoi sclerotia from rye substrate
   

P. tampanensis sclerotia from manure based bulk substrate
   

So now you have that leftover sub/very small stones mixture, ready to fruit.

Fruiting Procedures:



Cased Grain Substrate:

This is the easiest method to case the grains and fruit them as is.
I've had OK results by casing RGS.
Had great results by casing Rye.

P. mexicana/Jalisco fruiting on cased RGS
   

P. galindoi/ATL#7 fruiting on cased RGS
   

P. galindoi/ATL#7 fruiting on cased Rye Berries
   

   

They don't require a special casing layer, I've used pasteurized 50/50, 50/50+, Jiffy Mix and Plantation Seed Starter Mix with equally great results.
Make sure that you use a thin (~1/4") layer of your mix.
Spread it evenly on the top of the substrate and put it into your fruiting chamber.
I've used a SGFC and Greenhouse and they both perform good.
Keep high humidity and lots of FAE throughout the whole fruiting.
Keep your temperature between 65-80F.
I've had them to fruit on constant 65F and constant 80F and anywhere in between.
Light temperature of 6500K is preferred (although I experimented with a 8200K light
too with good performance as well) with a cycle of 12/12.

Pins should appear within 10-14 days in optimal fruiting conditions.

Grains Spawned to Bulk Substrate:

You can spawn your grains to any pasteurized manure or coir based bulk substrate.
My favorite mix is horse manure/straw/coir/vermiculite/gypsum (40/20/20/15/5).
Let your bulk colonize, case and fruit it as above.

P. mexicana/Jalisco rye berries spawned to pasteurized coir/coffee/verm/gypsum
   

   

P. tampanensis spawned to pasteurized horse manure/straw/verm/coir/gypsum
   

   

   

Fruiting BRF Cakes:

With this method I don't harvest the sclerotia from the substrate.
After full colonization birth the cake and wash off the vermiculite barrier.
After this I like to dry the cakes out in the front of the flowhood or a fan.
Dry it for a few hours at least or overnight.

P. tampanensis cakes drying
   

Please note that the above step is not necessary but IME it will give you a better
flush/pinset.

Now that the cakes are dry you can go ahead and dunk them for 24 hours.
Put them back in the jars and case/fruit in the same manner as with  grains (described above).


P. tampanensis cakes dunking and cased
   

   

P. galindoi fruits on BRF substrate
   

Alternative Fruiting Method:

If you're after the fruits and don't care about sclerotia this is the TEK for you!
Prepare a bulk substrate mix in a spawn bag and sterilize it,
then inoculate it directly with LC.
Here's how I prepare my bulk.

Shred the horse manure with a small food processor.
I can't believe that some people still breaking up manure with their hands...

Shredding the manure
   

Chop up some wheat straw and submerge it in water for a couple of hours.

Prepping wheat straw
   

Hydrate some coir, and get the vermiculite & gypsum ready.
I don't measure anything exactly just eyeball it.
It's not that important, just be somewhere in the ballpark of the above mentioned ratio...

Ingredients for the substrate
 

Now mix your dry ingredients (manure/verm/gypsum) then mix in coir and straw.
Hydrate the sub to field capacity and fill into gusseted spawn bags.
I fill the bags with 2-4 quarts of substrate.

Mixed and hydrated substrate


I love RRs idea with the tyvek sleeves inserted in the bags,
you don't have to worry about contamination while your PC cools.

Prepping the spawn bags
   

Fill your PC with the bags, and PC them for 120 minutes @15psi.

Bags before and after PCing
   

When the bags are cool I open them in the sterile laminar airflow and
inoculate the bulk with 20-60cc of LC.
Inflate it with the sterile air and tie them up.
When fully colonized, cut the top of the bag just above the substrate level.
Case it thin with your favorite casing layer and fruit it.

P. galindoi bag fully colonized, top cut off and cased
   

P. galindoi fruits on the above mentioned bag
   

   

Harvesting & Spore Printing:

Now that the pins developed into mature fruits (anywhere between 5-10 days)
you are ready to harvest them.
This is the same as you would do with any other species of mushroom,
but keep it in mind that these guys are pretty fragile.
Gently pull them off the substrate and set them aside.

P. galindoi harvest from BRF
   

Cut the caps off the stipe as close as you can and place cap onto
alcohol sanitized heavy duty aluminum foil.
Do this in your glovebox or front of your flowhood.

P. galindoi caps printing
   

An other good method is to use a plastic shoebox.
Take the lid, and lay a sheet of sanitized foil in it.
Put the caps on the foil and place the bottom of the box on the top of the lid.
You'll have a nice sheet of good quality prints.

P. tampanensis caps mass-printing on foil in a shoebox
   

Finished prints of P. tampanensis
   

Thanks for reading:yesnod:

Edited by stonesun (12/11/10 07:00 AM)

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InvisibleEvilMushroom666
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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun] * 3
    #13432036 - 11/04/10 12:23 AM (13 years, 4 months ago)

:bow2:
Amazing article, beyond words my friend.

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OfflineTemperfiddle
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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: EvilMushroom666] * 3
    #13432260 - 11/04/10 01:36 AM (13 years, 4 months ago)

:alot:

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InvisibleeLShaMukO
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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun] * 3
    #13432416 - 11/04/10 02:26 AM (13 years, 4 months ago)

Great guide stone sun !

its cool that you took the time to do this  .:awesomenod:

i sense a TC tag some day soon.


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InvisibleLeopardMan
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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun] * 4
    #13432446 - 11/04/10 02:51 AM (13 years, 4 months ago)

Excellent work, I am amazed! Stonesun is the man :murray:


--------------------



You have to die a few times before you can really live.

-Charles Bukowski-

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InvisibleFractalXplora
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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: eLShaMukO] * 3
    #13432467 - 11/04/10 03:07 AM (13 years, 4 months ago)

OMG just woke up and uploaded this thread to my brain, absolutly amazing thread and info Stonesun, this one gonna go down in history.
To cold here to do any cubes, so this is gold dust for me this info.

My only gripe is where is this dude's TC TAG!¬!!!!!!!

:headbang3:  :cheer:  :headbang3:  :congrats:  :congrats:  :congrats:


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OfflineLakotis
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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: FractalXplora] * 3
    #13432563 - 11/04/10 04:17 AM (13 years, 4 months ago)

I just learned many things. Thank you!


--------------------
"Capitalism is the astounding belief that the most wickedest of men will do the most wickedest of things for the greatest good of everyone."
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Invisiblehamloaf
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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun] * 3
    #13432624 - 11/04/10 05:08 AM (13 years, 4 months ago)

Thanks for constructing an up to date, comprehensive guide about the various ways to cultivate Sclerotia for us, stonesun.  Nicely laid out.


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

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Offlinedif
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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: hamloaf] * 3
    #13432743 - 11/04/10 06:50 AM (13 years, 4 months ago)

:thumbup: That was great, nicely formatted and easy to understand. :thumbup:


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sevot yhtils eht dna ,gillirb sawT`
ebaw eht ni elbmig dna eryg diD
,sevogorob eht erew ysmim llA
.ebargtuo shtar emom eht dnA

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Invisibleawakendone
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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun] * 3
    #13432763 - 11/04/10 07:04 AM (13 years, 4 months ago)

this is exactly what i was looking for like 2 months ago
such awsome write up too! you always deliver!
i wont be shaking my future jars now, as i was alredy feeling the same about it myself
you so deserve five shrooms for this :mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:
and i will second that you really should have a TC tag :thumbup:

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Invisiblestonesun
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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: awakendone] * 3
    #13432857 - 11/04/10 08:02 AM (13 years, 4 months ago)

Thank you guys for the compliments:super:

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InvisibleFractalXplora
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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun] * 3
    #13433936 - 11/04/10 01:07 PM (13 years, 4 months ago)

Stonesun, can I ask about your LC technique, why the dextrose on top of the DME,whats the reasoning behind this? do you start from agar wedges from initial plate or do a few transfers and pick a healthy looking piece? I been getting a lot of floaters in my LC's and not much success. Do u use a stir plate and if so, how long u stir in a 24 hour period?

Im thinking my Lc's are poor cause Im not stiring and filtering them? :shrug: not sure really.

Cheers.:thumbup:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: FractalXplora] * 3
    #13434015 - 11/04/10 01:24 PM (13 years, 4 months ago)

:bow2: awesome guide!! very nicely laid out. great pics. Def going into fav's. :handth:


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Offlinekrum
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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: ICdeadPeople] * 3
    #13434042 - 11/04/10 01:31 PM (13 years, 4 months ago)

:mushroom2:

Haven't seen any posts from you in a while, guess you've been busy. 

This is tremendous, thanks SS

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: krum] * 3
    #13434112 - 11/04/10 01:45 PM (13 years, 4 months ago)

:rockon:
:popcorn:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: total] * 3
    #13434393 - 11/04/10 02:44 PM (13 years, 4 months ago)

:nothingtoadd:


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:dragon:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Dragonaut] * 3
    #13435019 - 11/04/10 04:54 PM (13 years, 4 months ago)

I think i might have to go grab one of my spore prints and fire up some stones. Thanks for the inspiration, great write up.
Slart


--------------------
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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: slartibartfast] * 3
    #13435418 - 11/04/10 06:40 PM (13 years, 4 months ago)

:congrats::congrats::congrats:


--------------------
How I make spore prints
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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: andymc] * 3
    #13435609 - 11/04/10 07:19 PM (13 years, 4 months ago)

:header:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Base Icks] * 3
    #13435750 - 11/04/10 07:48 PM (13 years, 4 months ago)

super bad ass thread, this thing was superb.

What are the trip qualities of sclerotia producers like lets say... ATL 7?


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Psychedelics are the only capable thing of pushing one's mind to the edge of infinity....

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Doctopus] * 1
    #13435980 - 11/04/10 08:34 PM (13 years, 4 months ago)

Hey SS,

Thank you for taking the time to gather your excellent info
on this topic into one thread.

I have enjoyed following your grows, and now we have one
place to find all the data.

I hope to follow in your footsteps.  I have ATL7 and Jalisco
on grains right now, and Tampanensis on agar.

Anyone wanting to trade MexA for...well, anything I have,
please let me know.

Take care all,

JD


--------------------
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Myco-tek.org

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: FractalXplora] * 1
    #13436268 - 11/04/10 09:26 PM (13 years, 4 months ago)

Quote:

FractalXplora said:
Stonesun, can I ask about your LC technique, why the dextrose on top of the DME,whats the reasoning behind this?




The combination of malt extract and dextrose had been the most succesful method in
the history of sugar based LCs. LME by itself would give you poor results and lots of floaters.
Which leads to your next question.

Quote:

FractalXplora said:
I been getting a lot of floaters in my LC's and not much success.
Im thinking my Lc's are poor cause Im not stiring and filtering them? :shrug:





I suspect from your first question that you use LME only in your LCs.
LME tends to form tiny clumps when introduce it to boiling water.
These clumps are sediment caused by the caramelization of the sugar.
Reduce the LME, add dextrose and you'll get a much cleaner/better LC.
I've used to filter mine but don't bother with it anymore, as I see
no difference in performance.


Quote:

FractalXplora said:
Do you start from agar wedges from initial plate or do a few transfers...
Do u use a stir plate and if so, how long u stir in a 24 hour period?





I do both. Depends what I try to achieve.
And I always start on agar then wedge to LC or straight to grain jar.
I do use a stir plate.
When (very seldom) inoculate my LC with spores, I wait a week to let them germinate
then stir it constantly for a few days on ~200RPM.
If inoculate with LC or agar wedge I stir it right after inoculation 24/7
till I got a thick slurry at the same speed.
Hope it helps.

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun] * 1
    #13436365 - 11/04/10 09:45 PM (13 years, 4 months ago)

Very, Very cool article man! Very well written! I've been very curious about trying some stones lately and its great to see such a simple tek! Thanks

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Doctopus] * 1
    #13437544 - 11/05/10 06:53 AM (13 years, 4 months ago)

Quote:

Doctopus said:
super bad ass thread, this thing was superb.

What are the trip qualities of sclerotia producers like lets say... ATL 7?




IDK man, check out the Easier than Cubes thread. Lots of info on potency etc.

@JD - Thanks, if I finally get mexicana/A to fruit I'll send you a print - gratis...

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun] * 1
    #13437665 - 11/05/10 08:15 AM (13 years, 4 months ago)

"@JD - Thanks, if I finally get mexicana/A to fruit I'll send you a print - gratis... "

Hoo hoo!

I will have to get you back.

I bought two exotic packs from SW.....one of those ought
to please you in return.

Hoogashaga.....Hogwashegi....Hoogashocka...somthing like that.  ;0) 

Have you grown the Goliath Cambodginiensis before?

Well, thanks again for sharing.

JD


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Javadog] * 1
    #13437756 - 11/05/10 08:58 AM (13 years, 4 months ago)

:popcorn:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Javadog] * 1
    #13437836 - 11/05/10 09:27 AM (13 years, 4 months ago)

Quote:

Javadog said:
Hoogashaga.....Hogwashegi....Hoogashocka...somthing like that.  ;0) 





Just call it semperviva:wink:
I'm working with it for a while. I have two bags of sterile
bulk substrate and a few modified BRF/manure/straw
cakes ready to be cased.
And today I'm spawning 2qt of colonized rye on pasteurized bulk sub.
Different substrates, different methods. We'll see what can I get out of them.

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun] * 1
    #13442572 - 11/06/10 09:02 AM (13 years, 4 months ago)

This is one kick ass thread.
Loads of great info and your pics are really cool, nice and clear.:thumbup:

I started to read the "easier than cubes" thread 2 days after ordering a halloween goodie bag from spore works.
I could of kicked my self, should of ordered some Alt7 as well:mad:

I'll just have to wait a little while before I see any sclerotioa.

Great work Stonesun keep up the good work.:smile:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: cookie dude] * 1
    #13443325 - 11/06/10 12:35 PM (13 years, 4 months ago)

:thumbup:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Mr. Anderson] * 1
    #13443612 - 11/06/10 01:59 PM (13 years, 4 months ago)

Wow dude

sick tek :super:

Your fruiting pics are Legendary:feelsgoodman:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Pscientist] * 1
    #13445732 - 11/06/10 09:56 PM (13 years, 4 months ago)

Quote:

Pscientist said:
Your fruiting pics are Legendary




Lol...:archiebunker:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun] * 1
    #13446615 - 11/07/10 01:19 AM (13 years, 4 months ago)

Wow, very clear and instructive post.
No green house, my GF will finally be happy :smile:
Will try to grow some sclerotia now !

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: mill] * 1
    #13446864 - 11/07/10 03:40 AM (13 years, 4 months ago)

this just reinforces the thoughts in my mind of trading off my left nut in order to get these spores i think this would be a good thing to do when im tired of cubes


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun] * 1
    #13447031 - 11/07/10 05:51 AM (13 years, 4 months ago)

Quote:

stonesun said:
Quote:

FractalXplora said:
Stonesun, can I ask about your LC technique, why the dextrose on top of the DME,whats the reasoning behind this?




The combination of malt extract and dextrose had been the most succesful method in
the history of sugar based LCs. LME by itself would give you poor results and lots of floaters.
Which leads to your next question.

Quote:

FractalXplora said:
I been getting a lot of floaters in my LC's and not much success.
Im thinking my Lc's are poor cause Im not stiring and filtering them? :shrug:





I suspect from your first question that you use LME only in your LCs.
LME tends to form tiny clumps when introduce it to boiling water.
These clumps are sediment caused by the caramelization of the sugar.
Reduce the LME, add dextrose and you'll get a much cleaner/better LC.
I've used to filter mine but don't bother with it anymore, as I see
no difference in performance.


Quote:

FractalXplora said:
Do you start from agar wedges from initial plate or do a few transfers...
Do u use a stir plate and if so, how long u stir in a 24 hour period?





I do both. Depends what I try to achieve.
And I always start on agar then wedge to LC or straight to grain jar.
I do use a stir plate.
When (very seldom) inoculate my LC with spores, I wait a week to let them germinate
then stir it constantly for a few days on ~200RPM.
If inoculate with LC or agar wedge I stir it right after inoculation 24/7
till I got a thick slurry at the same speed.
Hope it helps.




Sure does man, thanks. Off to try that info just now :wink:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: FractalXplora] * 1
    #13447047 - 11/07/10 05:58 AM (13 years, 4 months ago)

Quote:

Wow, very clear and instructive post.
No green house, my GF will finally be happy :smile:
Will try to grow some sclerotia now !




x2


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: mill] * 1
    #13447273 - 11/07/10 07:44 AM (13 years, 4 months ago)

Quote:

mill said:
my GF will finally be happy :smile:






Look at that stonesun, you're even pleasing other guys girlfriends

Is there anything you can't do ?
:durrhurr:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Pscientist] * 1
    #13452568 - 11/08/10 07:50 AM (13 years, 4 months ago)

Found some more pictures, will update the OP in a few.

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun] * 1
    #13454773 - 11/08/10 04:53 PM (13 years, 4 months ago)

Hey stonesun. Nice guide to Sclerotia production. Approximately how much sclerotia do you yield from one quart jar of mexicana when using agar?

UKShroomer


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: ukshroomer] * 1
    #13455019 - 11/08/10 05:44 PM (13 years, 4 months ago)

Quote:

ukshroomer said:
Approximately how much sclerotia do you yield from one quart jar of mexicana when using agar?





The inoculant (agar wedge, spore syringe, LC or GLC) has no effect on the yield.
Unless of course it's an isolate.
Time however is a huge factor.
Yield greatly depend on how long will you let your jars go.
With mexicana (ms inoculation) I constantly got 100g+ of stones on rye berries
if I let them go for 3 months plus.

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun] * 1
    #13490388 - 11/15/10 10:26 PM (13 years, 4 months ago)

where do you keep your stone jars during col?
complete darkness, or some ambient light?

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: awakendone]
    #13491410 - 11/16/10 06:00 AM (13 years, 4 months ago)

I have a closet dedicated to spawn jars/bags, so they're sitting
in there.
I've used to keep 'em in a room on a shelf by a window
and seen no difference in performance.

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #13491787 - 11/16/10 08:50 AM (13 years, 4 months ago)

Awesome pictorial, thanks!!

Can anyone link to (or summarize) a quick dosing guide for both stones and fruits of sclerotia?


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: gornyhuy]
    #13494463 - 11/16/10 06:21 PM (13 years, 4 months ago)

Not me, I'm sorry.
Maybe someone else can chime in, although it's pretty off topic
in a sclerotia GROWING guide...:ohwell:

Edited by stonesun (11/16/10 06:39 PM)

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: gornyhuy]
    #13514241 - 11/20/10 12:22 PM (13 years, 4 months ago)

Quote:

gornyhuy said:
Awesome pictorial, thanks!!

Can anyone link to (or summarize) a quick dosing guide for both stones and fruits of sclerotia?




As much as you think you can handle lol.
Didn't know there was a set dosing to anything.


Awesome article. Can't wait to give these a try. Thinking I will do these along side the tubs as a side project


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: SuPaTriP]
    #13515963 - 11/20/10 06:38 PM (13 years, 4 months ago)

Let us know how they turn out when you try them!:super:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #13580028 - 12/04/10 01:15 AM (13 years, 3 months ago)

Hey Stonesun. Hows it going? Really nice guide. I just inoculated a bunch of jars with LC which I made from a clone. I shook them right away and they seem to be growing really fast. 2 are already fully colonized. I have one quick question. How long does it take from full colonization until I see truffles beginning to form?

Thanks
UKShroomer


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: ukshroomer]
    #13580624 - 12/04/10 07:14 AM (13 years, 3 months ago)

Quote:

ukshroomer said:
I have one quick question. How long does it take from full colonization until I see truffles beginning to form?





Within a week of full colonization.
What did you clone?
Sclerotia or fruit tissue?

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #13581051 - 12/04/10 10:18 AM (13 years, 3 months ago)

I cloned Sclerotia tissue.  I got the truffle from my local smartshop. I has been colonized for about a week now and I am not seeing any truffles forming. How long should I wait before worrying?
The mycelium is healthy white.


Thanks
UKShroomer


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun] * 1
    #13582471 - 12/04/10 05:10 PM (13 years, 3 months ago)

Stonesun, could you possibly update your tek with a little bit more detail about your casing technique?

I have some RGS jars of Ps. gallindoi/ATL#7 that are a few months old, with well-developed sclerotia, but I'm interested in fruiting one or two of those jars for prints.

Another tek here describes casing the straight grain spawn.  Is it better to spawn to coir or one of the other bulk substrate materials, in your opinion?

As I understand it, the RGS should be cased with ~1/4" 50/50 peat/verm, with lime to get the pH up to ~8.  Should I spawn to a 50/50 coir/verm first, let colonize, and then case immediately before fruiting?  Or should the mycelium be allowed to break the casing layer before fruiting?  Or else should I spawn and case at the same time a la cased cubes, and allow to break the casing layer before fruiting?  One sees so much different information around but you seem to be one of the experts at getting these to fruit.



I stupidly tried spawning to coir/verm without casing, like cubes, and got no pinning activity, :doh:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: andymc]
    #13582634 - 12/04/10 05:44 PM (13 years, 3 months ago)

Stonesun has said in this thread and told me personally that he doesn't let his galindoi casings sit at all and begins to fruit them immediately, I personally would advise spawning to a bulk sub instead of just fruiting cased grains

hope this helps


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: andymc]
    #13585670 - 12/05/10 01:15 PM (13 years, 3 months ago)

"Should I spawn to a 50/50 coir/verm first, let colonize, and then case immediately before fruiting?"

"Or should the mycelium be allowed to break the casing layer before fruiting?"

"Or else should I spawn and case at the same time a la cased cubes, and allow to break the casing layer before fruiting?"

These are good questions....I bet that they have been
covered by SS's grow threads,
but a reiteration would be appreciated.

I too have ATL7 jars, one at about 1.5 months and the other
at two weeks.  I hope to get some fruits from these jars.

Take care,

JD


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Javadog]
    #13586966 - 12/05/10 06:21 PM (13 years, 3 months ago)

Quote:

Javadog said:
"Or else should I spawn and case at the same time a la cased cubes, and allow to break the casing layer before fruiting?"






sorry but you must be doing something wrong, or you are slightly confused.. thats not how casings are traditionally applied.. The bulk sub is allowed to colonize, and then it is cased, it is up to the individual whether they allow the mycelium to break through or not, In terms of cubes


However in both cases colonization of the casing is not recommended and it defeats its purpose, which is to provide a humid micro climate close to the surface of the mycelium with good airflow , which is the purpose of a casing no matter the species, colonization limits the airflow of the casing layer

I personally have experience with spawning this species to straight verm and it gave decent results, more nutrients  and available moisture by means of a bulk sub can only help the colony of mycelium so I would suggest spawning to bulk sub



in summary , casing is applied after colonization directly before fruiting, and not allowed to colonize at all,

I dont mean to steal your thunder stonesun Im just trying to help JD out, and I hope you aren't offended JD im just trying to clear up some easily mistaken issues,

but by all means if you dont trust me wait for stonesun to answer :grin:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Pscientist]
    #13587170 - 12/05/10 07:13 PM (13 years, 3 months ago)

I was the one asking those questions.  In any case thanks for clearing that up.  I've never cased anything before.


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: andymc]
    #13587564 - 12/05/10 08:39 PM (13 years, 3 months ago)

oops my bad:bonghit:


:braindamage:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Pscientist]
    #13589448 - 12/06/10 09:02 AM (13 years, 3 months ago)

Quote:

ukshroomer said:
How long should I wait before worrying?





I wouldn't worry for another couple of weeks.
What was your substrate?

Quote:

andymc said:
Stonesun, could you possibly update your tek with a little bit more detail about your casing technique?
Is it better to spawn to coir or one of the other bulk substrate materials, in your opinion?





I might just do that.
Both straight cased grains and spawn to bulk works, I thought this was obvious from this thread.
If you want bigger, healthier fruits then spawn to bulk just like Pscientist suggested it.
I've spawned P. mexicana to coir and did well, but P. galindoi performed poor.
My favorite bulk now is horse manure/straw/coir/vermiculite/gypsum (40/20/20/15/5).
You got the rest right about the casing material and thickness.

Quote:

Pscientist said:
I dont mean to steal your thunder stonesun Im just trying to help JD out, and I hope you aren't offended




Not at all.

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #13590844 - 12/06/10 02:19 PM (13 years, 3 months ago)

Hey stonesun. Hows it going? My substrate was rye berries. I simply loaded the rye berries into the jars with the correct amount of water and sterilized for 1 hour. I also did a few jars using RogerRabbit's rye prep tek. I found that the jars I had prepared by simply putting the rye grain and water into jars actually colonized much faster than the ones using the RR method. I attribute this to the fact that the grains are softer and more puffed up making it easier for the mycelium to attach itself and start growing. The downside to this method is that the grains have to be shaken while still hot or they can clump together. When I make grain spawn for cubes and other species I still stick to the RR method. I am using 870 ml microboxes with homemade silicone injection ports. I do my inoculations in open air and I have not had a single contamination yet! I find that mexicana mycelium is very thin and tempermental. It is rather easy to get the LC into a syringe. I made some LC syringes of z-strain today and the needle got clogged 5 time while I was aspirating the mycelium.


The tek I used for my grain prep can be found here.
http://www.mushmush.nl/?page=begin\methods\making_grain_spawn


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OfflineOoBYCoO
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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: ukshroomer]
    #13608175 - 12/09/10 04:20 PM (13 years, 3 months ago)

:bow2:  :bow2:  :bow2:  :bow2:  :bow2:  :bow2:
I was just looking into this and here you are!  You're the man!

Hey, any chance you have a pdf of your guide?

Edited by OoBYCoO (12/09/10 04:24 PM)

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: OoBYCoO]
    #13608212 - 12/09/10 04:25 PM (13 years, 3 months ago)

:themoreyouknow: :curbyourenthusiasm:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #13608389 - 12/09/10 04:54 PM (13 years, 3 months ago)

"Let's Grow Sclerotia by stonesun" coming soon?
:smilingpuppy:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: total]
    #13608399 - 12/09/10 04:55 PM (13 years, 3 months ago)

Quote:

total said:
"Let's Grow Sclerotia by stonesun" coming soon?
:smilingpuppy:




:yesnod:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun] * 1
    #13608464 - 12/09/10 05:05 PM (13 years, 3 months ago)

Quote:

stonesun said:
Quote:

total said:
"Let's Grow Sclerotia by stonesun" coming soon?
:smilingpuppy:




:bigyesnod:



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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #13608651 - 12/09/10 05:38 PM (13 years, 3 months ago)

Quote:

stonesun said:
Quote:

total said:
"Let's Grow Sclerotia by stonesun" coming soon?
:smilingpuppy:




:yesnod:





Ill buy the first copy ! :murray:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Pscientist]
    #13608667 - 12/09/10 05:43 PM (13 years, 3 months ago)

I got the ball rollin and the idea out there...
Make it happen...
Then pass me my cut...

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #13616303 - 12/11/10 06:42 AM (13 years, 3 months ago)
Log in to view attachment

Well, ladies and gentlemen here's the Guide in Adobe Document Format (.pdf)
you can download it right to your hd.
I'd like to say thanks to OoBYCoO for the idea and to make this happen:super:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #13616527 - 12/11/10 09:02 AM (13 years, 3 months ago)

Thank you for taking the time SS!


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Javadog]
    #13616549 - 12/11/10 09:10 AM (13 years, 3 months ago)

Fantastic....One of the best writeups I have seen in my few years here. Thanks Stonesun.:awethumb:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Ozzy]
    #13617332 - 12/11/10 01:27 PM (13 years, 3 months ago)

Thanks for the shot-out stonesun!  No problem, it was my pleasure... check your e-mail b/c I sent you a surprise!

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: OoBYCoO]
    #13630152 - 12/14/10 05:39 AM (13 years, 3 months ago)

Stonesusn, I want to have your babies. Fully sick write up mate!! :shocked:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: _OttO_]
    #13632436 - 12/14/10 03:41 PM (13 years, 3 months ago)

:fonz:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: _OttO_]
    #13632494 - 12/14/10 03:55 PM (13 years, 3 months ago)

Quote:

_OttO_ said:
Stonesusn, I want to have your babies.




Hey, thanks man.
They are both in high school, will ship them out tomorrow:super:





































J/K...:rofldrunk:

Edited by stonesun (12/14/10 04:14 PM)

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #13632582 - 12/14/10 04:14 PM (13 years, 3 months ago)

Top-notch thread Stonesun. More folks should cultivate these, my fave.  Never had a succesful jalisco culture though...just tampanensis and mexicana strain A.

Great work. ~P~


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Psuper]
    #13655398 - 12/19/10 10:08 AM (13 years, 3 months ago)

I will also add my lips to your behind and say great thread, well done. 

I do have a question and you seem like you might be the guy to ask:

Can I clone sclerotia by adding directly to an LC? I have some Mex A jars about 3 months old now, popped one the other day and found 120ish grams of sclerotia, nice. I was gonna pop another soon and wondered if it would be worth putting a sterile, fresh stone into an LC or 3?

I have tried G2G before with these with poor results, I think they are less forgiving contaminant-wise than cubes due to the extended invitro period.

I have a few agar plates left but wanted to use them for another project. I'm guessing it'll be xmas or New Year before most places are shipping reliably and I can get more, especially with the weather over here..

I read the whole thread (and several others) but can't seem to find this mentioned, any thoughts??

Cheers all, keep up the good stuff SS! :peace:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: egodeathflux]
    #13655650 - 12/19/10 11:39 AM (13 years, 3 months ago)

I am betting that agar would be a better place to start.

There is no guarantee that sclerotia are clean.

You could try washing it briefly in H2O2.

Good luck!

JD


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Javadog]
    #13655909 - 12/19/10 12:42 PM (13 years, 3 months ago)

I'm almost certain agar would be a better place to start too...:tongue: However, in the abscence of available dishes, I might give it a shot unless someone has tried a few times and knows that it's a waste of time...??

I was thinking of removing a stone from a clean jar (pre-harvest) with heated tweezers and just dropping it in to some LC. I have plenty of stones available and no shortage of LC jars and honey. I think I'm gonna give it a whirl, will post results when I'm done. :syringe:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Javadog]
    #13655913 - 12/19/10 12:43 PM (13 years, 3 months ago)

:thumbup:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: egodeathflux]
    #13656250 - 12/19/10 01:57 PM (13 years, 3 months ago)

Quote:

egodeathflux said:
I was thinking of removing a stone from a clean jar (pre-harvest) with heated tweezers and just dropping it in to some LC.




I do this all the time, it works great.
Why wouldn't sclerotia be "clean" if you pull it from
a sterilized jar of sterilized substrate?

Quote:

egodeathflux said:
I think they are less forgiving contaminant-wise than cubes due to the extended invitro period.





Not so.
They are actually VERY forgiving.
I've harvested sclerotia with my bare hands and
separated the colonized rye.
Threw the grains in a cereal cup (didn't even washed it out), cased
with 50/50+ and it produced just fine without any contamination.
This being said I will never recommend the "Neglect Tek" to anyone!

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: egodeathflux]
    #13656408 - 12/19/10 02:36 PM (13 years, 3 months ago)

Quote:

egodeathflux said:
I think they are less forgiving contaminant-wise than cubes due to the extended invitro period.




I don't think I was being clear there.. I meant that G2G is more tricky as they sit for so long, althought the myc may dominate; if there's a single contam present it will probably show itself in that time period. I've had jars turn bad after 4 months fully colonised. I like to use LC or agar as I think the risk of airborne contams is less when transferring, not a big G2G fan really.

Cool, glad to know you've done it successfully, thanks man. I know the sclerotia will be clean, my concern was more whether it would produce myc easily. I will get started on my LC's now, hopefully silencing some doubters along the way.. lol (You know who you are!! :peace:) :syringe:


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"Atrophic interludes weave through my life far too often, for me to fight the biggest enemies"




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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #13656734 - 12/19/10 03:45 PM (13 years, 3 months ago)

"I do this all the time, it works great.
Why wouldn't sclerotia be "clean" if you pull it from
a sterilized jar of sterilized substrate?"

This make sense.  Thanks SS.

I suppose that grains from a grain jars are cleaner than a sample
from a birthed BRF cake.  I tried an LC from the latter without success,
but then again I was just getting started.

Good luck,

JD


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Javadog]
    #13656801 - 12/19/10 03:58 PM (13 years, 3 months ago)

Some of my stones turn almost a blackish brown color, Not like the normal stone brown imo. What would you say that is? I wouls suspect bacteria?

Thats happened in nearly all my jars, from 3-6 months old. :shrug:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Base Icks]
    #13656891 - 12/19/10 04:14 PM (13 years, 3 months ago)

Quote:

Base Icks said:
Some of my stones turn almost a blackish brown color, Not like the normal stone brown imo. What would you say that is? I wouls suspect bacteria?

Thats happened in nearly all my jars, from 3-6 months old. :shrug:




Pics please! I definitely had some pretty black streaks through some of my healthy ones, usually against the glass it seems, I'm sure you know they can do some funky colour schemes.

All my late-stage contams have been white speckles that form on the stones and substrate. To be honest I'm not 100% sure mine were contaminated, they were between 4 and 6 months and just weren't quite right so I tossed them. This has only happened to me with G2G which is why I avoid it.


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: egodeathflux]
    #13656976 - 12/19/10 04:25 PM (13 years, 3 months ago)



Same jar, Both sides.

I also just found a pf cake with a stone that grew around a fungus gnat larva and sandwitched it on the glass. NOM NOM NOM.

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Base Icks]
    #13657065 - 12/19/10 04:38 PM (13 years, 3 months ago)

Those look normal to me :shrug:

Some of the stones do get pretty dark brown


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: andymc]
    #13657096 - 12/19/10 04:41 PM (13 years, 3 months ago)

THat pics like a month old I think. It looks darker to me know :shrug:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Base Icks]
    #13657986 - 12/19/10 07:33 PM (13 years, 3 months ago)

I've been lead to believe sclerotia jars can look all kinds of funky.  So far mine have all been good :thumbup:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: andymc]
    #13658187 - 12/19/10 08:24 PM (13 years, 3 months ago)

HA!  That looks like a claussen pickle jar to me.

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: krum]
    #13658192 - 12/19/10 08:27 PM (13 years, 3 months ago)

i think it is.

or a mustard jar


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: k00laid]
    #13658222 - 12/19/10 08:39 PM (13 years, 3 months ago)

I just looked at the jars I have, but Ick's don't have an expiration date like mine do.  Do pickles not expire in the UK?

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: krum]
    #13658566 - 12/19/10 09:53 PM (13 years, 3 months ago)

Great guide :smile:

I had a small amount of ATL spore solution, so I made an LC and colonized some bags from that.

Despite making sure the grains were totally dry before I PC'd them, there's a fair bit of condensation in the bags, so I'm a bit worried about that.  However, I'm hoping the LC will colonize quickly and everything will be good :smile:

Do you stonesun(or anyone) have some estimates of the possible yield from casing the grains leftover after harvesting the sclerotia?  I'm trying to decide if it'll be worthwhile, I'm using 10 lbs of rye.


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Ubitsa]
    #13659604 - 12/20/10 03:47 AM (13 years, 3 months ago)

Mason jars are 30 bucks a case here. I dont have that kinda :money: atm. Besides I have 6 liter pc's. I could fit two liter jars in them at a time. Not really worth it imo.

So I just reuse sauce jars.

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Base Icks]
    #13659792 - 12/20/10 06:12 AM (13 years, 3 months ago)

I think those stones look ok too, some of mine were that dark at least. For some reason my jars always produce hundreds of tiny stones, they never seem to get as large as yours! My yields are good, just in little pieces..

Just gotta make some emergency repairs to my glovebox then gonna throw some stones in the LC's I cooked last night. Will probably add the pics to this thread if Stonesun doesn't mind me hi-jacking it. :syringe:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: egodeathflux]
    #13671357 - 12/22/10 03:29 PM (13 years, 3 months ago)

thank you!! finally, why couldnt i find this two days ago, downloadable and everything, again THNKYOU


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: ellomello]
    #13673412 - 12/22/10 09:58 PM (13 years, 3 months ago)

Quote:

egodeathflux said:
Will probably add the pics to this thread if Stonesun doesn't mind me hi-jacking it. :syringe:




I don't mind at all, go for it!:yesnod:

Quote:

ellomello said:
thank you!! finally, why couldnt i find this two days ago, downloadable and everything, again THNKYOU




:highfive:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #13823792 - 01/20/11 11:09 PM (13 years, 2 months ago)

This is a damn good thread.
Saved to favorites.


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: KaptKid]
    #13823838 - 01/20/11 11:16 PM (13 years, 2 months ago)

Congrat's on finally getting that well deserved TC tag!!!  :bigyesnod:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: OoBYCoO]
    #13824330 - 01/21/11 01:34 AM (13 years, 2 months ago)

Quote:

OoBYCoO said:
Congrat's on finally getting that well deserved TC tag!!!  :bigyesnod:



:highfive:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #13824481 - 01/21/11 02:45 AM (13 years, 2 months ago)

I've only read the OP, but I'm sure the whole thread is awesome.  Great job, reallly, and congrats.  Maybe people will start growing more of these out and actually fruit them instead of just getting the stones and wasting the rest.  Very informative and up to date.

This thread = win for many a growers!:thumbup:
Stay stoned, sun:learyharvard:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: egodeathflux]
    #13824927 - 01/21/11 08:37 AM (13 years, 2 months ago)

All the LC's turned out great (except one which went a little cloudy...), I think it's a great way to grow this strain as you can be fairly genorous with LC and get them going fast. I wondered if the sclerotia would out-perform the grains so did a little experiment.

I tried half the LC jars with colonised grains and the other half with actual sclerotia (10 jars total, 5 of each). There was no noticable difference between the two. I just tranferred with tweezers in a still air box. I can't find my camera and don't think pics would really add much. LC was just Acacia honey and mineral water, simple but great results.


Stonesun I was being lazy and didn't re-read your OP, maybe you could add a note about sclerotia LCs? I'm fairly sure even a semi-fresh stone (properly cleaned) would produce viable tissue from it's centre. I imagine some folks can get stones but not colonised grain, say from a vendor...

I kinda feel like a douche asking you to add anything to this awesome thread. :wink:


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Offlinefig
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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #13824962 - 01/21/11 08:56 AM (13 years, 2 months ago)

great stone guide and nicely put together too.

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: egodeathflux]
    #13825004 - 01/21/11 09:13 AM (13 years, 2 months ago)

Good point ego, but do you reckon it would be better technique (perhaps less contamination-prone?) to break the stone open with your fingers, then take a small tissue sample from inside it?  Like we do with fruitbodies?

I haven't tried culturing any stones yet, personally.


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: andymc]
    #13825305 - 01/21/11 10:42 AM (13 years, 2 months ago)

TBH I'm not sure which way would be better as I've only tried this once so far. If these all colonise without problem then I'd be hesitant to change the method really, if it ain't broke etc etc...

I have a purpose bought pair of extra-long needle-ended tweezers, I think if you're in and out quickly the risk of contam may be lower this way due to less time exposed to open air, even if it's in GB/SAB. This is best done immediately before harvesting stones from jar and fruiting.

Personally I never like to handle anything I'm gonna grow/culture. My contam rates are pretty darn low, less than 5% I'd say, I'm very careful though. Saying that I've seen your print tek and you're obscenely clean!!

I think that in the instance of say ordering fresh-ish stones online, and making an LC from there, it might be a good way to go. A quick soak in H2O2 followed by an extraction of internal tissue sounds feasible to me, I could be wrong.


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: egodeathflux]
    #13825509 - 01/21/11 11:35 AM (13 years, 2 months ago)

Once again....THANK U  :congrats:  :congrats:  :congrats:  :congrats:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: egodeathflux]
    #13826329 - 01/21/11 02:08 PM (13 years, 2 months ago)

Maybe try a variation of CloneUFC's clone tek?

Take a fully hydrated stone, dip in h2o2, place in new ziploc and close it. In a few days you will see new myc growing from the stone. Transfer to agar or whatever and you are good-to-go.

I have some dried Mex-A stones in the fridge. Plan on picking a good one and letting it soak in spring water for 3 days or so then doing this procedure for practise at isolation.


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Shaggy Shaman]
    #13827345 - 01/21/11 05:25 PM (13 years, 2 months ago)

Post your results and let us know. I think I'd still prefer going straight to LC unless you wanna go to agar first. Even in that instance maybe a straight-forward tissue>agar would be preferable, the fewer transfers the fewer contams IME. I think most agar plates would provide sufficient moisture to hydrate a small stone.

I also think stone soaked in H2O2 then dropped straight into LC would be fine to hydrate it, this way it happens in a sterile environment.

I think any info on reviving dry/semi-dry stones would be useful for everyone. Not being a doubter, be interested in what you come up with. :thumbup:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: egodeathflux]
    #13830607 - 01/22/11 09:08 AM (13 years, 2 months ago)

You got it. I'll pick one out today and start the experiment!


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Shaggy Shaman]
    #13892812 - 02/02/11 09:47 AM (13 years, 1 month ago)

i just prepared my 3rd jar of rgs (actually its a grass seed mix) and did like it said in the first post (2:1 rgs:water/coffee by volume), but i feel like its too much liquid in there. as i mentioned, i already got 2 jars that are chilling out for 3 weeks now and there i didnt actually measure the amount of water or rgs, i just did as i felt was right - and ended up having too much water in it & the myc struggling to colonize the bottom parts (if you got any suggestions to help me out here, shoot). but with the 2:1 ratio it seems to me that its even more water. or does the rgs suck it up in the 2 hours it sits?


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: egodeathflux]
    #14008756 - 02/22/11 05:26 PM (13 years, 1 month ago)

Quote:

egodeathflux said:
I also think stone soaked in H2O2 then dropped straight into LC would be fine to hydrate it, this way it happens in a sterile environment.

I think any info on reviving dry/semi-dry stones would be useful for everyone. Not being a doubter, be interested in what you come up with. :thumbup:




Welp! It took about a month for growth to show, but here it is!

First the jar of dry stored stones.


The stone hydrating:


The stone placed in a zip-lock.


Pics of the stone TODAY!



The stone was harvested in May of 2010, dehydrated, and placed in a desiccant chamber. Then stored in glass and refrigerated until last month. So we now can see that sclerotia are excellent lifepods and are capable of coming back to life.


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Shaggy Shaman]
    #14009161 - 02/22/11 06:42 PM (13 years, 1 month ago)

:super:  Excellent experiment!!!

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Shaggy Shaman]
    #14009326 - 02/22/11 07:14 PM (13 years, 1 month ago)

Woah thats sweet

never underestimate the powers of fungi :mushroom2:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Shaggy Shaman]
    #14009687 - 02/22/11 08:15 PM (13 years, 1 month ago)

Quote:

Shaggy Shaman said:
First the jar of dry stored stones.






That's a nice haul, congrats :congrats:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: andymc]
    #14012254 - 02/23/11 07:58 AM (13 years, 1 month ago)

Thanks! I still haven't done anything with the stones! I've been too occupied with normal mushies (PE & OR). I guess eventually I will grind them up and use them in chocolates.


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Shaggy Shaman]
    #14013618 - 02/23/11 01:38 PM (13 years, 1 month ago)

i would like to know if anyone tries nothing but plain brown or white rice for growing P. Mexicana (A).  i appreciate all the fancy teks, but personally, i am not into mass-production or another time-consuming hobby.  i just like to grow once every few years for personal enjoyment, and i don't use a pressure cooker, nor do i have the space for one (i live very minimally in a very little home with very little space).  i have done PF tek successfully in my one and only 1.5L stainless pot with a glass dish cover using 1/2 pint jars.  my personal interest is to know if anyone else has tried just plain white/brown rice (w/water of course) in a 1/2 pint jar, no pressure cooker, and then injecting some Karo LC into it to grow sclerotia.  or, why this would definitely be a bad idea.

i'd like to see bare-bones poor-man simplest possible primitive sclerotia tek - that works consistently, of course :smile:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: i18rabbit]
    #14013889 - 02/23/11 02:13 PM (13 years, 1 month ago)

PF Tek works great for sclerotia! Shoot up some jars and put them away for later.
There's less verm than you'd think. You can make tea and filter it if it's still too much for you.


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: i18rabbit]
    #14014281 - 02/23/11 03:03 PM (13 years, 1 month ago)

my thinking is this:

i have seen instructions that say "75ml of whole white rice + 100ml of water": but this is for white rice (1:1.5 rice:water ratio), brown rice seems to be 1:2.25, rice:water.  i think you want the grains cooked to "perfection", not too dry, not overly wet or overly cooked, ie, as plump and fluffy as possible without bursting and sticky.  so, i would think package directions would be the way to go.

--

1/2 pint jar with: 60ml whole brown rice, 135ml water,
with a tiny hole in the lid covered with electrical tape.
the jar is then covered with aluminum foil and placed into
a 1.5L stainless pot with a glass plate cover, with 2 inches
of bath water, then at the lowest heat possible on a gas stove
(flame barely alive), it will boil gently in several minutes,
and according to directions, brown rice should be done in
about 30 minutes (white rice, about 20min), and it should be
sterile too.  probably a good idea to make it a few times
until the method is done well, before inoculation.

at least, i never had contaminants doing this with PF-tek.

then when everything is cool, take some P. Mexicana-A
from Karo-LC culture (20ml Karo + 200ml water), using
a sterile syringe (boiled in foil for 20min like Karo LC),
and after heating the needle red hot, some LC is drawn and
injected thru the electrical tape on the lid of the rice jar
(a major contamination-danger step, because of the air rushing
in to fill the vaccuum in the jar).  the rice jar is then
quickly covered again with the foil and a new piece of
electrical tape is placed over the hole just made in the
LC lid with the syringe.

EDIT: alternatively, this looks like a better method, ie, cooking rice first, then sterilizing it with a layer of vermiculite on top ... http://forums.mycotopia.net/rice-teks-whole-grain/10558-whole-rice-substrate-verm-cased.html

--

ps. i haven't come across this, but not having a fancy glove box or anything, in the past i just ran an ozone machine.  after that, i simply used a little 4W UVC light in the operating area (should wear some kind of glasses if you do that).  i don't know the facts on how well they sterilize the surrounding air, but i never had contaminants in 20+ LCs/PF-teks, doing all transfers in open air with no special sanitizing (other than flaming a needle), never using anything more than 1/2 pint mason jars, electrical tape, and aluminum foil sterilized in a little pot with glass plate cover.  either i was lucky, or lived in an unusually clean home, or Ozone/UVC lighting does a really good job of sterilizing a work area in open air.  of course, i always made sure to have freshly cleaned hands.

as a test, i did sterilize 1/2 pint of white rice 3 months ago as stated above, and let it sit in a cabinet without any innoculations, and to this day, there is no contaminants growing in it.  i would hope that 1/2 pint jar would produce 1 dose of sclerotia.

Edited by i18rabbit (02/24/11 04:49 AM)

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Doc_T]
    #14014508 - 02/23/11 03:32 PM (13 years, 1 month ago)

i would never eat Vermiculite :smile:  that is why i am thinking more like "whole brown rice".  i don't think Vermiculite is poison, but i think it's full of Aluminum and other things i am not sure that i would want to eat.  but ... then again, that healing clay people eat is full of similar minerals, and people in Haiti make mud pie :smile:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: i18rabbit]
    #14014515 - 02/23/11 03:33 PM (13 years, 1 month ago)

NO UV! UV can kill you or cause cancer. No UV!


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Doc_T]
    #14014649 - 02/23/11 03:55 PM (13 years, 1 month ago)

not to get off topic, but UVC has no penetration power at all, plus it creates ozone, and much less than 1mm of almost any material will block it (even a few feet of air, depending on the lights power), and it can't penetrate skin deeply enough to cause any bad cancer - but it can cause cataracts (as in mountain climbers).  UVC does not alter DNA in the same way as UVB (which has greater penetration power, and can cause cancer), and the damage UVC causes to DNA has not been shown to be cancer causing in studies (the body either repairs the DNA well, or the cell dies).  though i did read one bad report about it causing cancer once in nude mice which have very thin skin, and they were subjected to UVC in an intense and brutal fashion.  we should aim for the truth - for example, i was flustered the other day to see the "organic health nut crowd" say that Propylene Glycol was a big evil and carcinogen in many foods and products including "aircraft de-icing & anti-freeze!!!" - when it is about as safe and non-toxic as water.  i wanted to reply "water is chemical used in toilets and sewage treatment!  and you drink it!  yuck!"

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: i18rabbit]
    #14016917 - 02/23/11 10:25 PM (13 years, 1 month ago)

Amazing article! Thank you so much.
MM


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: mango man]
    #14019956 - 02/24/11 01:19 PM (13 years, 1 month ago)

Going to have to give these a shot.

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Shaggy Shaman]
    #14029355 - 02/26/11 08:55 AM (13 years, 1 month ago)

Great post Shaggy. I love when a question gets answered, and thoroughly. :bow2:


I was just going to add that anyone who can make an LC for stone production should do so.

The ones I noc'd and mentioned earlier in the thread are going mad. They were producing stones at around 15% colonisation, so guessing I should get a decent haul at some point.

LC is the way forward with strain, the myc is fatter, faster and healthier too. :thumbup:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: egodeathflux]
    #14079197 - 03/07/11 12:46 AM (13 years, 25 days ago)

This is just awesome.  Thanks a bunch. 


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Edited by fbi365 (03/23/11 02:30 AM)

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: egodeathflux]
    #14079922 - 03/07/11 07:49 AM (13 years, 25 days ago)

Quote:

egodeathflux said:
Great post Shaggy. I love when a question gets answered, and thoroughly. :bow2:


I was just going to add that anyone who can make an LC for stone production should do so.

The ones I noc'd and mentioned earlier in the thread are going mad. They were producing stones at around 15% colonisation, so guessing I should get a decent haul at some point.

LC is the way forward with strain, the myc is fatter, faster and healthier too. :thumbup:




im gonna knock up 2 jars today, one with my LC i made some weeks ago. its a nice fuzzy ball in there :grin:
hope im successful, the last jar got cobweb - when i realized it it was already sporulating like mad...T_T


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: godisanastronaut]
    #14080434 - 03/07/11 11:18 AM (13 years, 25 days ago)

I guess, given the price and rarity of the strain...


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Edited by fbi365 (03/23/11 02:02 AM)

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: fbi365]
    #14083483 - 03/07/11 08:22 PM (13 years, 25 days ago)


Basically, what tactic do you use to stretch this stuff out as much as possible?

Thanks ya'll,
Your humble apprentice


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Edited by fbi365 (03/23/11 02:30 AM)

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: fbi365]
    #14083521 - 03/07/11 08:31 PM (13 years, 25 days ago)

Is a few drops to agar an option?

Normally, I would suggest a BRF cake as the safest way to get a print,
but Jalisco is not typical.  It kicked my butt the first time, but I
kept some genes so I am trying again.

Good luck!

JD


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Javadog]
    #14083525 - 03/07/11 08:32 PM (13 years, 25 days ago)

I'd vote agar as well.

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Base Icks]
    #14083552 - 03/07/11 08:38 PM (13 years, 25 days ago)

I am trying to take it one step at a time.  LC seems like a good option b/c I have a stir plate and stir bar for yeast cultivation, basically I understand the concept.
P.S. I am new to this...


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Edited by fbi365 (03/07/11 08:39 PM)

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: fbi365]
    #14083777 - 03/07/11 09:18 PM (13 years, 25 days ago)

I love LCs.

The fact is that they can be tricky though.

A BRF cake in a SGFC would be the safest route, but we need a Jalisco
expert to comment.

Good luck,

JD


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Javadog]
    #14083804 - 03/07/11 09:24 PM (13 years, 25 days ago)

you are saying the best way of stretching this is to try and get some prints...


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Edited by fbi365 (03/23/11 02:28 AM)

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: fbi365]
    #14083921 - 03/07/11 09:44 PM (13 years, 25 days ago)

Ahh....yes, I am a spore-whore.  I want prints!

If stones are good for you, then rye berries are the way to go.
(or wheat berries, or some other grain that you can look forward to eating ;0)

In fact, I think that I could see my way clear to trying MS on grains,
with the intention using G2G transfers to more jars.  This way if only
one rye berry jar from the MS inoculation succeeds then you can still expand.

Does this jive?

JD


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Javadog]
    #14083957 - 03/07/11 09:52 PM (13 years, 25 days ago)

Yeah, dog, this jives just fine.  This makes great sense since the stones develop in the grain any way.  It eliminates a step...

Thanks


--------------------



Edited by fbi365 (03/23/11 02:27 AM)

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: fbi365]
    #14084075 - 03/07/11 10:16 PM (13 years, 25 days ago)

How much spore solution do you inject (one quart jars per RR's RGS Tek)?


--------------------



Edited by fbi365 (03/23/11 02:27 AM)

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: fbi365]
    #14084191 - 03/07/11 10:44 PM (13 years, 25 days ago)

1-2 CC'S

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: fbi365]
    #14084193 - 03/07/11 10:44 PM (13 years, 25 days ago)

Bro, if you could open the jars and use an eye dropper, then I would say
about 1-3 drops....but you can't.  As little as 0.5 cc ought to work.

Do not shake these after inoculation!  Do not even jostle them about.

You will want the spores to be in the same area so that they can mate
and make the die-carry-aught-ick mycelium that will fruit for you.

Good luck,

JD

P.S. Ok, I'll give it a shot: dykaryotic (diploid vs haploid)

P.P.S. dIkaryotic    ....missed it by THAT much!


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Edited by Javadog (03/07/11 10:46 PM)

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Javadog]
    #14084200 - 03/07/11 10:46 PM (13 years, 25 days ago)

0.5ml

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Base Icks]
    #14084218 - 03/07/11 10:52 PM (13 years, 25 days ago)

haha thanks dudes.


--------------------



Edited by fbi365 (03/23/11 02:25 AM)

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: fbi365]
    #14084245 - 03/07/11 11:00 PM (13 years, 25 days ago)

Very nice Stone I haven't seen this thread before.

Did you start using Manure/coir because of me?  I thought I was the only one that did that with stone producers and even told it was not as good as just casing by a couple Tc's.
CH

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: CH HELL]
    #14084964 - 03/08/11 02:48 AM (13 years, 24 days ago)

why not proceed with normal technique and use the stones / possible fruits later to inoculate
further jars (in a glove box or in front of a flow hood)?


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: godisanastronaut]
    #14085468 - 03/08/11 07:32 AM (13 years, 24 days ago)

Like a g2g, but with stones instead of grain?


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Doc_T]
    #14086164 - 03/08/11 11:30 AM (13 years, 24 days ago)

Yeah that works great Doc. See my earlier posts in this thread.

I still think you should try and make some LC straight away fbi365, the difference in growth from MS compared with

LC from the same MS is ridiculous. I have stones 3"x3" in jars that are only 90% colonised. Far bigger stones

from the same MS syringe>LC in 3 weeks than I got in 3 months not using the LC. Hope that makes sense, I'm a

little tipsy. :thumbup:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) *DELETED* [Re: Javadog]
    #14086901 - 03/08/11 02:13 PM (13 years, 24 days ago)

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: bootster]
    #14086947 - 03/08/11 02:22 PM (13 years, 24 days ago)

Never shake sclerotia jars IME. The myc doesn't recover as healthy and stone production is stalled/slowed.


--------------------

"Atrophic interludes weave through my life far too often, for me to fight the biggest enemies"




"Standing on the corner of 5th and Vermouth"


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) *DELETED* [Re: egodeathflux]
    #14087022 - 03/08/11 02:35 PM (13 years, 24 days ago)

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: bootster]
    #14087040 - 03/08/11 02:39 PM (13 years, 24 days ago)

it will start out white, then start changing colors, i love the looks of stone producers mycelium

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    #14087047 - 03/08/11 02:40 PM (13 years, 24 days ago)

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: bootster]
    #14087062 - 03/08/11 02:42 PM (13 years, 24 days ago)

I've shaken ATL#7 jars without any problem.


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: bootster]
    #14087074 - 03/08/11 02:43 PM (13 years, 24 days ago)

i've never tried shaking, i normally just leave them alone to do their thing.

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: LeopardMan]
    #14087176 - 03/08/11 02:58 PM (13 years, 24 days ago)

Quote:

LeopardMan said:
I've shaken ATL#7 jars without any problem.





With the LCs I'm using I'm seeing stones form at 15-20% colonisation, I think shaking will effect the stones. Never tried ATL, only Mex A. :shrug:


--------------------

"Atrophic interludes weave through my life far too often, for me to fight the biggest enemies"




"Standing on the corner of 5th and Vermouth"


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: godisanastronaut]
    #14087956 - 03/08/11 05:31 PM (13 years, 24 days ago)

Quote:

godisanastronaut said:
why not proceed with normal technique and use the stones / possible fruits later to inoculate
further jars (in a glove box or in front of a flow hood)?




Seems like there's a hundred and one ways to make this stuff last. 


--------------------



Edited by fbi365 (03/23/11 02:25 AM)

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: fbi365]
    #14089571 - 03/08/11 10:20 PM (13 years, 24 days ago)

You don't need a flowhood to do good sterile work.


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Doc_T]
    #14090960 - 03/09/11 05:19 AM (13 years, 23 days ago)

huk.. i think my LC got cobweb :frown: i hate those guys


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: godisanastronaut]
    #14104774 - 03/11/11 04:27 PM (13 years, 21 days ago)

Wheat Berries instead of Rye?  Any anticipated differences/problems?


--------------------



Edited by fbi365 (03/23/11 02:23 AM)

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: fbi365]
    #14105316 - 03/11/11 06:34 PM (13 years, 21 days ago)

this thread is bad ass thanks for posting.


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: fbi365]
    #14105997 - 03/11/11 08:59 PM (13 years, 21 days ago)

Quote:

fbi365 said:
Jalisco on Wheat Berries instead of Rye?  Any anticipated differences/problems?




They'll do fine.


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Javadog]
    #14154748 - 03/20/11 04:31 PM (13 years, 12 days ago)

loving this thread, those are some lovely harvest pictures :P 

Someone  was discussing on here about starting a liquid culture by dropping a stone into a liquid culture jar, working in a still air box. another possible method would be to take a core sample.

you take a sterile syringe and suck up a little of a new liquid culture, wipe a stone with iso, stick the syringe through the stone and back out. and inject this back into your liquid culture jar, the liquid in the syringe helps push out the piece of stone.

I read this on a another thread on here and to me that would seem the most sterile way of working as you would never have to open the liquid culture jar.

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: chronicdruid]
    #14154847 - 03/20/11 04:53 PM (13 years, 12 days ago)

Interesting idea, I can envision the syringe getting blocked though and you wouldn't be able to flame the needle..

I use flamed/alcohol'd fine tweezers to move grains/stones to LC jars, I think it's the quickest way with least air exposure. I imagine it would take a minute or 2 to get your core sample extracted. Open air = :sad:

Though as you say, not opening the LC jar is always gonna be a bonus..

Not being a doubter, post some results if you try it and out and let us know.

Might be a good project for the IRF...


--------------------

"Atrophic interludes weave through my life far too often, for me to fight the biggest enemies"




"Standing on the corner of 5th and Vermouth"


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: egodeathflux]
    #14254067 - 04/07/11 04:11 PM (12 years, 11 months ago)

great job wish i could get you to come to my house and teach me a lil haha i injected 10 jars and no contam and this is my first time i juat put them in the fruiting chamber i cant wait till they are done i want to try it some diff ways and try some experiments but i would like someone with experience with me

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: chal420]
    #14254226 - 04/07/11 04:40 PM (12 years, 11 months ago)

Yo I got some stone jars I knocked up in like the beginning of Feb, they're producing but seem a little dry, what should I do, just leave em?





I want to inject sterile water into it but Idk, is it cool? Strain A Mex btw.

I just wanted to try em out cause I thought they were cool and I've never seen em before.


--------------------


Disclaimer: All posts are completely fictional and or for educational purposes only.

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Mr. Anderson]
    #14362835 - 04/27/11 06:35 PM (12 years, 10 months ago)

Thanks for this StoneSun, awesome stuff. You do good work. 54U.


--------------------
 
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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Mr. Anderson]
    #14377262 - 04/30/11 12:01 PM (12 years, 10 months ago)

Those look fine for Mex A, there will still be moisture in the grains, don't worry IMO.


--------------------

"Atrophic interludes weave through my life far too often, for me to fight the biggest enemies"




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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: egodeathflux]
    #14377762 - 04/30/11 01:38 PM (12 years, 10 months ago)

Thanks, there was some moisture in the bottom and I tilted it and got the excess water on the top of the grains by rolling the jar. I got it to roll around a little so hopefully the top will colonize. The bottom of the grain is colonized to where its pulling away from the dry and squeezing moisture out bedtime.

I wanted the top to fully colonize cause I wanna try and spawn them for prints.


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Mr. Anderson]
    #14481529 - 05/19/11 10:28 PM (12 years, 10 months ago)

Oh my gawd! Thank you sooo much for the suggestion to read this tek! It now resides in my favorites/bookmarks. WOOT!


--------------------
Please assume any and all prints exchanged are "WILD" in nature; and thus, should NOT be considered ASEPTIC...

NOTE:  Please excuse my brevity, as it is a bitch 'n' a half and slow as hell to type on this here phone :frown:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: HorizonSpawn]
    #14482101 - 05/20/11 12:32 AM (12 years, 10 months ago)

Yes, SS has come out with textbooks logs, one after the
other, and often difficult and exotic species/strains.

I am trying his Gymnopilus right now.  It is not the easiest
thing I have tried.  ...I need to read his log again.  ;0)

Good luck!

JD


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Javadog]
    #14524860 - 05/28/11 03:10 AM (12 years, 9 months ago)

Gyms are some of the easiest of woodlovers to grow.

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: mycot]
    #14575215 - 06/07/11 03:57 PM (12 years, 9 months ago)

Nothing to add, just want to watch this thread and don't know how without commenting. Cheers and excellent work.

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: ragehead]
    #14578397 - 06/08/11 06:19 AM (12 years, 9 months ago)

:whathesaid::nothingtoadd:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: shroomzi8]
    #14579667 - 06/08/11 12:53 PM (12 years, 9 months ago)

Just wanted to add a little note about the benefit of patience when fruiting Mex A (I assume most sclerotia species are similar... correct me if I'm wrong, SS).

I harvested some stones about 4 weeks ago, left a few with the grain then did a straight casing, no added substrate.

After 3 1/2 weeks I had seen nothing, not even myc poking through the casing. I woke up yesterday to find 6 huge pins(compared to my previous attempts/successes) popping their beautiful heads up. This morning there were a couple more and they look super-healthy and vigorous.

I know these guys will take longer than cubes, but I was kind of giving up hope for this tray.

So basically my message is: hang in there and give the little suckers time, you may be rewarded even if things look less than spectacular for a few weeks.

I'm just using a SGFC, with a little extra fanning/misting each day. Can't wait to harvest, gonna try a stones+Mex A combo, hope they will compliment each other nicely. :cool: :thumbup:


--------------------

"Atrophic interludes weave through my life far too often, for me to fight the biggest enemies"




"Standing on the corner of 5th and Vermouth"


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: egodeathflux]
    #14579738 - 06/08/11 01:10 PM (12 years, 9 months ago)

Quote:

egodeathflux said:
Just wanted to add a little note about the benefit of patience when fruiting Mex A (I assume most sclerotia species are similar... correct me if I'm wrong, SS).

I harvested some stones about 4 weeks ago, left a few with the grain then did a straight casing, no added substrate.

After 3 1/2 weeks I had seen nothing, not even myc poking through the casing. I woke up yesterday to find 6 huge pins(compared to my previous attempts/successes) popping their beautiful heads up. This morning there were a couple more and they look super-healthy and vigorous.

I know these guys will take longer than cubes, but I was kind of giving up hope for this tray.

So basically my message is: hang in there and give the little suckers time, you may be rewarded even if things look less than spectacular for a few weeks.

I'm just using a SGFC, with a little extra fanning/misting each day. Can't wait to harvest, gonna try a stones+Mex A combo, hope they will compliment each other nicely. :cool: :thumbup:



:borat:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: OoBYCoO]
    #14583416 - 06/09/11 04:31 AM (12 years, 9 months ago)

Quote:

P. mexicana/Jalisco rye berries spawned to pasteurized coir/coffee/verm/gypsum




how much coffee?

thanks.


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: eLShaMukO]
    #14587300 - 06/09/11 07:44 PM (12 years, 9 months ago)

I've used coffee grinds at about a 10:1 ratio to verm/coir/manure.(as in 10 parts verm/coir/manure to 1 part coffee...) That was with cubes though..

Don't add too much, it is more of a substrate additive than an actual bulk..

I'm sure someone can give you more accurate info, but I believe that is about right.. Best thing to do is always try a few different mixes and post your results!! :thumbup:


EDIT: POST # 1,000!! What am I doing with my life?? lol.


--------------------

"Atrophic interludes weave through my life far too often, for me to fight the biggest enemies"




"Standing on the corner of 5th and Vermouth"


Edited by egodeathflux (06/09/11 07:45 PM)

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: egodeathflux]
    #14589275 - 06/10/11 07:37 AM (12 years, 9 months ago)

Quote:

egodeathflux said:
EDIT: POST # 1,000!!




I was hoping we'll have some mexicana pictures in your 1000th post:wink:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #14589319 - 06/10/11 07:57 AM (12 years, 9 months ago)

Quote:

EDIT: POST # 1,000!! What am I doing with my life?? lol.




:cheers: learning good skills ego!


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #14600920 - 06/12/11 01:27 PM (12 years, 9 months ago)

Quote:

stonesun said:
Quote:

egodeathflux said:
EDIT: POST # 1,000!!




I was hoping we'll have some mexicana pictures in your 1000th post;)




Ask, and ye shall receive! Already picked the 1st few, but here's the rest of the 1st flush.






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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: egodeathflux]
    #14612139 - 06/14/11 01:16 PM (12 years, 9 months ago)

well done egodeathflux :smile: I bet you were pleased to get some lovely little fruits in the end :laugh:

I have got some jars inoculated with mexicana A. i used a multi-spore lc. It has been months now and still no stones!!! :frown:  I got the spores from MRCA. The jars all fully colonized and then just don't seen to want to make stones :S

Could it be that i have ended up with a non fruiting set of strains? I have 50 jars like this XD  although i used 3 different liquid cultures to inoculate them. I cant believe my luck.

If anyone could make any suggestions i would appreciate any help, i might post pics at later date, but they are fully colonized just with out any stones :frown:


  My plan of action now is to put some spores from the syringe onto agar and try and isolate a stone producing strain :smile:


great thread sunstone :smile: i read your beer brewing thread as well :smile: awesome


heres my lazy little jars :frown:  ...

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: chronicdruid]
    #14612326 - 06/14/11 01:45 PM (12 years, 9 months ago)

Quote:

chronicdruid said:
well done egodeathflux :smile: I bet you were pleased to get some lovely little fruits in the end :laugh:

I have got some jars inoculated with mexicana A. i used a multi-spore lc. It has been months now and still no stones!!! :frown:  I got the spores from MRCA. The jars all fully colonized and then just don't seen to want to make stones :S

Could it be that i have ended up with a non fruiting set of strains? I have 50 jars like this XD  although i used 3 different liquid cultures to inoculate them. I cant believe my luck.

If anyone could make any suggestions i would appreciate any help, i might post pics at later date, but they are fully colonized just with out any stones :frown:


  My plan of action now is to put some spores from the syringe onto agar and try and isolate a stone producing strain :smile:


great thread sunstone :smile: i read your beer brewing thread as well :smile: awesome


heres my lazy little jars :frown:  ...





I'm sorry bro, but that does not look like sclerotia mycelium. I think you should try casing one of those jars and see what kind of fruit pops up. My bet is that you will get cubes.


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Edited by overstand (06/14/11 01:49 PM)

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: overstand]
    #14615316 - 06/15/11 12:30 AM (12 years, 9 months ago)

Second pic looks contammed at bottom.

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Base Icks]
    #14615323 - 06/15/11 12:33 AM (12 years, 9 months ago)

They should look like this...

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: OoBYCoO]
    #14615520 - 06/15/11 01:37 AM (12 years, 9 months ago)

I am geussing it is cube myc and someone mislabeled the spore print.

OooBy-  The bag in the top left looks great the others don't look as heatly.  Are they all the same culture?
CH

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: CH HELL]
    #14615615 - 06/15/11 02:11 AM (12 years, 9 months ago)

Yeah.  :shrug:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: OoBYCoO]
    #14616516 - 06/15/11 09:34 AM (12 years, 9 months ago)

thanks for the replies, I may well have to fashion a SGFC and try and get some fruits! The mycelium did start very whispy to begin with but has become thicker over the months of incubation at room temp.

    For now all I can do is drool over everyone else's sclerotia pics mmmmmmmmmmmmmmmmmm :P

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: chronicdruid]
    #14616972 - 06/15/11 11:21 AM (12 years, 9 months ago)

I'd agree that myc looks too thick and cotton-y for sclerotia strains, though my only experience is with Mex A... Often times with LC I've had stones forming at 50%ish colonization.. Got my original syringe from MRCA too, it worked fine..

If it turns out to be cubes then I'd hit them with an email and see what they can offer you. Usually pretty good customer service from sponsors, mention you're on Shroomery, that always helps. Good luck.

BTW I got most of that tray pictured above covered in fruits now, should get a decent harvest after all! :cool:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: egodeathflux]
    #14618333 - 06/15/11 04:08 PM (12 years, 9 months ago)

well it would be the simplest reason why i havent seen any stones. I still have some of the original spore syringe mex A. so I may try and isolate a stone producing strain from it soon.

I dont have much time atm but I've managed to whip up a few little casings, just with 2 inch spawn covered in a fine layer of verm casing. I'll whack them in a SGFC in a couple of days time and see if I can get any shrooms. 

good job on your harvest ego :smile:

ps. when you made your liquid culture, was it from an isolate, clone, or just from the spore syringe? anyways take it easy brovah, and enjoy that harvest!

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: chronicdruid]
    #14622710 - 06/16/11 01:30 PM (12 years, 9 months ago)

Made several batches of LC, some from fresh stones, others from colonised grains.. Maybe some from the ms syringe too, I can't recall.... Got a little sloppy/lazy with that batch, they all seem happy though! :cool:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: egodeathflux]
    #14627104 - 06/17/11 08:43 AM (12 years, 9 months ago)

Hey SS or anyone else, can you give me a vague idea of potency of Mexicana fruits compared to P. Cubensis/P. Sem?

I'm thinking of trying some out this weekend and not sure how many to eat or not eat.. Can't really find a definitive answer with the search engine, though I seem to recall reading somewhere they were slightly more potent gram for gram than cubes.. Any thoughts? Some of my fruits are as fat as cubes, so really happy with how they turned out in the end. :cool:

I was thinking a trial run of around 2.5g would be a good place to start. Will post results here when I have some 1st hand info of my own.

I've said it before and I'll say it again, amazing thread StoneSun, if only every grow guide were as comprehensive. :bow2:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: egodeathflux]
    #14628439 - 06/17/11 02:15 PM (12 years, 9 months ago)

some people say they are llike pan cyans


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: egodeathflux]
    #14635081 - 06/18/11 07:34 PM (12 years, 9 months ago)

Quote:

egodeathflux said:
I've said it before and I'll say it again, amazing thread StoneSun, if only every grow guide were as comprehensive. :bow2:




Hey thanks!
And thanks for participating in it.

Quote:

egodeathflux said:
Hey SS or anyone else, can you give me a vague idea of potency of Mexicana fruits compared to P. Cubensis/P. Sem?





Unfortunately I can't help you in potency questions.:shrug:
I've heard different views on the matter, so please make sure to post your expriences!

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #14691746 - 06/29/11 01:25 PM (12 years, 8 months ago)

Ok I have made a SGFC and a few casings, just got my first few pins so hopefully a species I.D will be possible.

Ive made a thread with pictures that I will update if and when I can get some mature specimens. http://www.shroomery.org/forums/showflat.php/Number/14691683#14691683

hope you had a good time with your fruits Ego and I look forward to any future threads of yours Stonesun, very informative and lots of lovely pics  :hatsoff:  :headbang:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: chronicdruid]
    #14698742 - 06/30/11 08:16 PM (12 years, 8 months ago)

Looks like cubensis.:shrug:
CH

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: CH HELL]
    #14698922 - 06/30/11 09:03 PM (12 years, 8 months ago)

Quote:

CH HELL said:
Looks like cubensis.:shrug:
CH



:whathesaid:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: egodeathflux]
    #14699175 - 06/30/11 09:56 PM (12 years, 8 months ago)

Quote:

egodeathflux said:
Hey SS or anyone else, can you give me a vague idea of potency of Mexicana fruits compared to P. Cubensis/P. Sem?







I have had Mex-A fruits as potent as Pans but the next time I tried them it took 6g(dry fruit bodies) to get to lvl3:shrug:.  So after many doses with these I say less than cubes but sometimes with fresh you will get more than you expect. 
Another note I have NEVER hit a lvl5 trip with any stone producer and I have taken enough stones and fruit bodies.  So I wouldn't worry about going over board with these.

CH

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: CH HELL]
    #14699444 - 06/30/11 10:42 PM (12 years, 8 months ago)

Will instant coffee work just as good as brewed coffee for sclerotia on RGS?


--------------------
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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: OMGitsBigCheeze]
    #14699500 - 06/30/11 10:54 PM (12 years, 8 months ago)

"just as good" == I doubt it.

It might add some of the same nutrients though...

You will want to weaken it, a great deal.

Use grounds if you can.  Mushrooms love them.

JD


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: CH HELL]
    #14702478 - 07/01/11 03:44 PM (12 years, 8 months ago)

Cheers CH, been too busy to try anything yet. Sounds like fresh might be a more interesting way to go then... :thumbup:

I can't imagine getting anywhere near level5 on stones alone, know someone who did though. Hats off to Base Icks and the 50-grammer-slammer. lol


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: egodeathflux]
    #14703095 - 07/01/11 05:46 PM (12 years, 8 months ago)

Sorry StoneSun but I need to share this.
The largest stone I have ever grown it is over 20g my small scale only goes to 20g  but My larger scale has a dead battery so I couldn't get a total weight.

CH

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: OMGitsBigCheeze]
    #14704314 - 07/01/11 10:09 PM (12 years, 8 months ago)

Quote:

OMGitsBigCheeze said:
Will instant coffee work just as good as brewed coffee for sclerotia on RGS?




I use a weak instant coffee solution when hydrating my RGS grains, and I get very good sclerotia.

All my real coffee gets drank. :grin:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: andymc]
    #14732300 - 07/07/11 05:09 PM (12 years, 8 months ago)

Just ate some Mex A fruits... keeping notes will report back/edit this post in a few hours.

:mushroom2::rocket::hug:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: egodeathflux]
    #14732354 - 07/07/11 05:23 PM (12 years, 8 months ago)

:awecid:  Good vibes to you!  :super:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: egodeathflux]
    #14732381 - 07/07/11 05:29 PM (12 years, 8 months ago)

waiting for the report :smile:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: eLShaMukO]
    #14735629 - 07/08/11 10:42 AM (12 years, 8 months ago)

A little preview of the ongoing project.
Psilocybe galindoi/ATL#7 fruiting in a 27qt monotub:

 

   

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #14737189 - 07/08/11 04:40 PM (12 years, 8 months ago)

interesting

do those two wholes are always with out poly fill ?


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #14737942 - 07/08/11 07:46 PM (12 years, 8 months ago)

That is going to make a minor forest!

I look forward to more.

JD


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #14738612 - 07/08/11 10:18 PM (12 years, 8 months ago)

Epic thread man, thanks heaps. But im still a little lost with the spore print to spore syringe leap. So are spores in spore print syringes still active? Im in australia and have had the joyous experience of trying philosophers stones ONCE and would love to grow them to enlighten many ppl on the deep journey they took me on but the only samples allowed through customs are spore print samples "for research only". Maybe someone knows an aussie distributor so i can avoid the whole custom problem....


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: mistamonsta]
    #14738659 - 07/08/11 10:28 PM (12 years, 8 months ago)

Quote:

mistamonsta said:
Epic thread man, thanks heaps. But im still a little lost with the spore print to spore syringe leap. So are spores in spore print syringes still active? Im in australia and have had the joyous experience of trying philosophers stones ONCE and would love to grow them to enlighten many ppl on the deep journey they took me on but the only samples allowed through customs are spore print samples "for research only". Maybe someone knows an aussie distributor so i can avoid the whole custom problem....



sorry, the question was supposed to be are spore prints still active and how do i make a spore syringe. Got all excited at seeing this thread and my fingers couldnt keep up with my brain. Also, what is the optimum growing temperature? Or is that info available elsewhere? Serious noob here btw, any help appreciated


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: mistamonsta]
    #14738749 - 07/08/11 10:47 PM (12 years, 8 months ago)

Quote:

mistamonsta said:
Quote:

mistamonsta said:
Epic thread man, thanks heaps. But im still a little lost with the spore print to spore syringe leap. So are spores in spore print syringes still active? Im in Australia and have had the joyous experience of trying philosophers stones ONCE and would love to grow them to enlighten many ppl on the deep journey they took me on but the only samples allowed through customs are spore print samples "for research only". Maybe someone knows an aussie distributor so i can avoid the whole custom problem....



sorry, the question was supposed to be are spore prints still active and how do i make a spore syringe. Got all excited at seeing this thread and my fingers couldnt keep up with my brain. Also, what is the optimum growing temperature? Or is that info available elsewhere? Serious noob here btw, any help appreciated





Welcome to the Shroomery! Yes, spore prints are active. Here is a link to some must see videos for noobs. One of the videos shows you how to make a spore syringe from a spore print. I am pretty sure that anyone of the sponsors would send you a spore print in Australia. Good luck!


http://www.mushroomvideos.com/


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: overstand]
    #14738990 - 07/08/11 11:53 PM (12 years, 8 months ago)

Spore prints aren't active... that's why they are legal (except in CA, ID, and GA).  If they were active vendor would not be allowed to sell them nor us to trade them amongst ourselves.

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: OoBYCoO]
    #14739376 - 07/09/11 01:49 AM (12 years, 8 months ago)

You should also have no troubles purchasing a syringe as it's the same deal as a print. Spores in water. Also there are plenty of teks here to make syringes from prints, but here is the quick and dirty way.
1. Sterilize syringe, needle, shot glass, etc
2. Put Needle, syringe, shot glass, distilled water, print in glove box
3. Pour water in shot glass
4. Lightly scrape spores into shot glass with water using your needle or inoculation loop (if you have one)
5. Mix solution up with needle in shot glass
6. Suck up water and spores into syringe
7. Follow this guide
8. Enjoy the amazing experience of tripping on your own home grown fun
9. Give many shroom rating to OP and props to this amazing community
10. Give back by taking clean prints and sharing with friendly shroomerites like myself... hey I can try right?

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: egodeathflux]
    #14739602 - 07/09/11 04:23 AM (12 years, 8 months ago)

Quote:

egodeathflux said:
Just ate some Mex A fruits... keeping notes will report back/edit this post in a few hours.

:mushroom2::rocket::hug:





So it took me a fewweeks to find the time but I now have some 1st hand info on the strength of Mex A fruits.

I took 1.4g, 12 large dried fruits. They are definitely stronger than cubes, though not quite as strong as Pan. Cyans/Tropicalis. I would equate the 1.4g to around 2.-5-3g of Cubensis.

The overall high/effect was very similar to the stones with the addition of far more pronounced and bright visuals.

The come up was fairly mellow and presented itself with uplifting body high and general euphoria. Found a definite drop off in potency at exactly the 2 hour mark, I put this down to the smaller size of the fruits and presumably an increased digestion rate compared with larger cubes.

Taste of fruits was similar to cubes though not as pronounced, easy to eat.

They seemed less introspective and more bouncy than cubes, CEV were more fractals than full on shifting scenes or real images. Still fun to watch and drift with. OEV's were less strong than CEV, generally just gentle flowing of surroundings accompanied by very enhanced neon/bright colours.

I will probably move up to 2.5-3g next time, in another few weeks, I can't trip too often anymore. :crazy2:

:peace:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: eLShaMukO]
    #14740266 - 07/09/11 10:17 AM (12 years, 8 months ago)

Quote:

eLShaMukO said:
interesting

do those two wholes are always with out poly fill ?




Yes.
That's my normal mono-procedure.
For colonization I tape up the 4 lower holes,
and stuff polyfill in the two upper holes.
Then for fruiting I remove the tape and stuff
those holes with poly and leave the other two
without.

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #14741653 - 07/09/11 04:12 PM (12 years, 8 months ago)

thanks :smile:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: eLShaMukO]
    #14746040 - 07/10/11 02:52 PM (12 years, 8 months ago)

I am a bit confused about the casing.  Perhaps someone can help.  I have a nearly colonized bulk substrate of Coir/Verm/Gypsum the species is P. Mexicana/Jalisco.  Do I add the casing layer when I introduce into fruiting conditions?  Or is that casing layer supposed to be colonized?


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: fbi365]
    #14746235 - 07/10/11 03:18 PM (12 years, 8 months ago)

Quote:

fbi365 said:
Do I add the casing layer when I introduce into fruiting conditions?  Or is that casing layer supposed to be colonized?




Yes, you case it when your bulk is fully colonized.
Introduce fruiting right after that.

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: fbi365]
    #14746285 - 07/10/11 03:30 PM (12 years, 8 months ago)

This is an interesting question.

In the not-too-distant past it seems that the community
resolved on a process where the casing was incubated, and
then patched before the grow was put into fruiting conditions.

It seems that there many opinions these days, with some
not casing cubes at all, and some trying various techniques
like late casing, etc

I currently move to fruiting conditions when I case.

Good luck,

JD


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Javadog]
    #14746350 - 07/10/11 03:39 PM (12 years, 8 months ago)

Quote:

Javadog said:
I currently move to fruiting conditions when I case.





:yesnod:
Same here!

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Javadog]
    #14746364 - 07/10/11 03:42 PM (12 years, 8 months ago)

Quote:

Javadog said:
This is an interesting question.

In the not-too-distant past it seems that the community
resolved on a process where the casing was incubated, and
then patched before the grow was put into fruiting conditions.

It seems that there many opinions these days, with some
not casing cubes at all, and some trying various techniques
like late casing, etc

I currently move to fruiting conditions when I case.

Good luck,

JD




Sometimes I wait a couple of days, sometimes I move to fruiting conditions right after casing (both for cubes and sclerotia). I didn't notice any difference.


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: LeopardMan]
    #14747642 - 07/10/11 08:07 PM (12 years, 8 months ago)

Thanks SS.  Happy to have the quick reply.  You are a stone god and a sun god!


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: fbi365]
    #14752683 - 07/11/11 05:50 PM (12 years, 8 months ago)

I'm still experiencing failures with ATL#7, wherein I get small pinsets that fail, then after a dunk I get some nice heavy pinsets, but they also fail.  I've gotten so close to having them reach maturity (2-3 inches tall, caps starting to open) but never had one fully mature.  It's frustrating.  I'm now trying a bit less FAE, and more light.  I've also just fruited colonized rye grains of these for the first time, instead of RGS.

My question: when I spawn cubes to coir, I give the top coir covering a good misting before I put it away to colonize.  I've been doing the same when I first case my ATLs and put them into fruiting.  From reading your guide, SS, you don't mention doing that.  It seems certain to me that misting the fruits, once they've broken the casing, is a no-no; but what about mising the casing layer before that?

These suckers seem pretty thirsty, in that my really good pinsets seem to come after a dunk.  So, I'm confused about whether to mist.

The tray I put in the FC tonight, I didn't mist.


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: andymc]
    #14753260 - 07/11/11 07:52 PM (12 years, 8 months ago)

Well, andy it's kinda the same debate with Panaeolus species.
Some say misting is a no-no (after pinning), some swear by it.
I've done it both ways - both ways succeeded.
I've ALWAYS mist the casing layer prior to pinning, and
after I see pins, well it's a matter of how I feel about it.
In this lates mono-grow I did not mist at all after pinning (5th day without).
They seem to do just fine!


   

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #14754859 - 07/12/11 12:45 AM (12 years, 8 months ago)

I can add that I also heard of the never-mist-the-pins thing
and one of my ways around it, at least in my own mind,
is to really saturate the casing right before the fruiting
begins.

...but I have also watered fruits while they were growing.

I am sorry to hear of your continuing difficulties Andy.

Good luck!

JD


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Javadog]
    #14756398 - 07/12/11 11:26 AM (12 years, 8 months ago)

I've been misting my Mex A. fruits for weeks, they are still flushing nicely. They are in a SGFC and I give it a good mist most days after removing the trays. I'll then check the trays, touch the casing and if it's not soft on top, they get a couple seconds from a pressure mister, doesn't seem to be hurting them at all. :cool:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #14765682 - 07/13/11 11:41 PM (12 years, 8 months ago)

Quote:

stonesun said:
Quote:

fbi365 said:
Do I add the casing layer when I introduce into fruiting conditions?  Or is that casing layer supposed to be colonized?




Yes, you case it when your bulk is fully colonized.
Introduce fruiting right after that.




So I peeked into my mono today and say several pins ranging from 1/4 inch to an inch.  It was still in colonization.  Should I still case it?  Will it destroy the pins?  What should I do?


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: fbi365]
    #14772359 - 07/15/11 12:06 PM (12 years, 8 months ago)

Casing before 100% colonisation seems to lead to contams 100% of the time IME.

I would probably let them grow and pick them. Case when sub has done it's thing. Never done them in a mono though, only trays.


--------------------

"Atrophic interludes weave through my life far too often, for me to fight the biggest enemies"




"Standing on the corner of 5th and Vermouth"


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: egodeathflux]
    #14772399 - 07/15/11 12:13 PM (12 years, 8 months ago)

Yeah stonesun that mono is pretty gangsta, but then again all your grows are.  :super:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #15360404 - 11/12/11 01:10 PM (12 years, 4 months ago)

I just started a Psilocybe Mexicana (Jalisco), using this Tek as

reference.  I'm using WBS, that was inoculated w/ a multispore from a

sponsor on 11/1/11. So far I have seen no growth in any of the

10qts.  I have never worked with Sclerotia & usually see some

sort of growth by now.  It there a target temp for colonization?

Will I have a better result with Rye & a LC?

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Eburger]
    #15361055 - 11/12/11 03:37 PM (12 years, 4 months ago)

mexicana(jalisco) is a poor stone producer with decent fruiting genetics

mexicana(A) is a good stone producer.

LC its just a another vector for contamination and an unnecessary step when you start from spores.

waiting one day for spores to germinate ? its at least between 3 days and 3 weeks , its a gamble.


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: eLShaMukO]
    #15361916 - 11/12/11 07:31 PM (12 years, 4 months ago)

You mis-read, I started Nov 1st.  But I will wait & see.  Also, I am

interested in some stones, but I am more interested in fruiting these

Thanks for the reply.

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Eburger]
    #15361919 - 11/12/11 07:32 PM (12 years, 4 months ago)

oh i see, i apologize.

make a thread when you can, this is a very interesting species you have

good luck .


--------------------

Edited by eLShaMukO (11/12/11 07:33 PM)

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #15361955 - 11/12/11 07:44 PM (12 years, 4 months ago)

thanks!


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Eburger]
    #15362039 - 11/12/11 08:09 PM (12 years, 4 months ago)

I've lost my Jalisco culture a while ago, and having trouble
lately to produce a good culture on agar.
Even the promising ones do not perform well on grains:shrug:
Wait a little more though...

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #15362072 - 11/12/11 08:17 PM (12 years, 4 months ago)

I'm about to order some sclerotia strains. Which one should I order p mexicana jalisco(only one in stock), or p tampanensis?


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: OregonChronic]
    #15362079 - 11/12/11 08:19 PM (12 years, 4 months ago)

What is your main goal?
Sclerotia, fruits or both?

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #15362084 - 11/12/11 08:21 PM (12 years, 4 months ago)

Both I guess


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: OregonChronic]
    #15362094 - 11/12/11 08:23 PM (12 years, 4 months ago)

Quote:

stonesun said:
Here's my experience on the matter (on a scale 1-10 10 being the best):

P. galindoi/ATL#7  - stone production 7
                                - fruit production 8
P. mexicana/Jalisco - stone production 2
                                - fruit production 9
P. mexicana/A      - stone production 10
                                - fruit production 1
P. tampanensis      - stone production 5
                                - fruit production 9

In other words if I'd want sclerotia only, I'd go with mex/A.
If I want the best over all stone/fruit producer, gotta go with ATL#7.
Tampanensis being my second choice.
With the mex/Jalisco got very few stones but fruited beautifully.



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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun] * 1
    #15362123 - 11/12/11 08:31 PM (12 years, 4 months ago)

Alright :stoned: I think I'm goin with ATL#7 strain


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #15364180 - 11/13/11 11:13 AM (12 years, 4 months ago)

Quote:

stonesun said:
... having trouble lately to produce a good culture on agar.
Even the promising ones do not perform well on grains:shrug:
Wait a little more though...




This describes my Jalisco experience pretty well.  I have started
new spawn jars using some new genetics from a good cultivator,
so hope springs eternal.

Good luck,

JD


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Javadog]
    #15470010 - 12/05/11 02:55 PM (12 years, 3 months ago)

Thanks. This is Awesome!

:raveface:


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: psyShroomer]
    #15608817 - 01/03/12 03:44 AM (12 years, 2 months ago)

excellent info thanks


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #15647394 - 01/10/12 09:40 PM (12 years, 2 months ago)

Thanks, great info guys. I'm definitely going to try this next.

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: riseup]
    #15647491 - 01/10/12 10:03 PM (12 years, 2 months ago)

Absolutely amazed. Thank you.

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Javadog]
    #15652909 - 01/11/12 10:53 PM (12 years, 2 months ago)

Quote:

Javadog said:
This describes my Jalisco experience pretty well.  I have started
new spawn jars using some new genetics from a good cultivator...




How's the Jalisco doing, JD?

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #15653294 - 01/12/12 12:33 AM (12 years, 2 months ago)

Hey SS,

They are going well.

I got some clean quarts, and have two cycles of sacks going strong.

First up are four sacks that must be at six weeks.  They are getting
bumpy on the surface, and will be cased tomorrow.

Here is one:


Next up is six pretty much identical blocks that are clean at about
three weeks and should make it fine.

Then I have nine additional quarts that I G2Ged to and that are clean so far.

So, I expect Jalisco to fall, and right soon at that!

Take care,

JD

P.S. Are you working these genetics too?


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Javadog]
    #15691731 - 01/20/12 08:03 AM (12 years, 2 months ago)

Wow !!

What a guide :thumbup:

Have to try it ...


  thank you Stonesun

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Teva]
    #15691739 - 01/20/12 08:05 AM (12 years, 2 months ago)

:popcorn:
This is the best guide for all stone producing mushies

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: UnnamedGrower]
    #15691965 - 01/20/12 09:26 AM (12 years, 2 months ago)

:popcorn:

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Fishermanj]
    #15782324 - 02/09/12 03:39 AM (12 years, 1 month ago)

Could I get some ideas on how to use sclerotia with bags? I don't think it's been covered in the thread....

I've read that LC is a risky way of going about inoculating bags as you can never know it's clean, and just shooting the spores takes ages to colonize....so the options as I see it are:


- Do a LC, hope for the best
- Do a LC, knock up a PF cake to check for contams
- Make a small (4-500ml ish) jar of grain*, then pour that into bag?
- Do a PT tek style cake to check for contams, crumble it into bag??

*not sure if it is possible to do small grain jar straight from spores, was trying to skip the jars tbh as it brings all sorts of filter questions into the mix which the bags would solve by having built-in filters.

Those are my current ideas, I'm also unsure about if it would be ok to crumble fully colonized jars as they might be producing stones by that point??

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: GoldenArrow]
    #15841192 - 02/21/12 03:52 AM (12 years, 1 month ago)

Will Milton work to sterilize a stone, and/or will it damage it? Time to try my hand at cloning soon...

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #16037211 - 04/03/12 06:33 AM (11 years, 11 months ago)

Is it jar lid + filter + jar ring OR jar ring + filter?


--------------------

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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: 1ve5w4hu]
    #16037488 - 04/03/12 09:04 AM (11 years, 11 months ago)

If I understand the question...

If you use just a synthetic filter disk and a jar ring,
your grains will dry out.

Good luck,

JD


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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: 1ve5w4hu]
    #16037492 - 04/03/12 09:06 AM (11 years, 11 months ago)

Quote:

1ve5w4hu said:
Is it jar lid + filter + jar ring OR jar ring + filter?




Its Jar lid(Flip it updside down, seal up) + filter + jar ring...

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InvisibleFishermanj
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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: total]
    #16040200 - 04/03/12 08:01 PM (11 years, 11 months ago)

Quote:

total said:
Quote:

1ve5w4hu said:
Is it jar lid  filter  jar ring OR jar ring  filter?




Its Jar lid(Flip it updside down, seal up)  filter  jar ring...





I prefer bags.  What size and how many holes do you put in your jar lids under the filter discs for those of you who use them?

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InvisibleJavadog
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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: Fishermanj]
    #16040526 - 04/03/12 09:06 PM (11 years, 11 months ago)

I have been using one 1/4" hole.

I have confirmed that four such holes will lead to
dried out grain on the top.

(I know that some use 2)

Good luck,

JD


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

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Invisiblefutureshock
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Re: A Comprehensive Sclerotia Cultivation Guide - From Spore Print To Spore Prints (VER 1.0) [Re: stonesun]
    #16045161 - 04/04/12 08:29 PM (11 years, 11 months ago)

Great piece of work, sunstone. Thanks for your excellent effort putting this guide together!

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