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 cubino
Curious
Registered: 05/19/10
Posts: 18
Last seen: 13 years, 3 months
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 antimicrobial plants myc friendly ? 
#13393773 - 10/27/10 03:01 AM (13 years, 5 months ago) |
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Is there antimicrobial stuff that I could use in a bulk substrate ?
I was thinking about making a test with:
- tea tree oil
- vinegar
- grapefruit seeds
- oregano
- rosemary
- sage
somebody had experiences ?
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 andymc
cocoa beetles from zanzibar
 
Registered: 11/25/09
Posts: 2,395
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 Re: antimicrobial plants myc friendly ? [Re: cubino]
#13393829 - 10/27/10 03:46 AM (13 years, 5 months ago) |
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Is there a reason you feel you need to do this, or is it just for scientific purposes?
Several of those things are acidic. You're going to be changing the pH of the substrate.
Fully-colonized spawn should be able to take control of the substrate without any extra help, provided the spawn isn't contaminated and the bulk material has been properly pasteurized.
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How I make spore prints
Trade List
My flow hood
If he asks me "Did you have a good time?" I'll say,
"Get the lights, Mr. Grim Reaper" -odds
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LeopardMan
Constantly changing
Registered: 09/21/09
Posts: 5,467
Loc: A tree house
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Re: antimicrobial plants myc friendly ? [Re: cubino]
#13393918 - 10/27/10 05:45 AM (13 years, 5 months ago) |
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Not needed. Correct sterile procedures and correct temperatures will be enough, believe me.
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You have to die a few times before you can really live.
-Charles Bukowski-
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cubino
Curious
Registered: 05/19/10
Posts: 18
Last seen: 13 years, 3 months
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Re: antimicrobial plants myc friendly ? [Re: LeopardMan]
#13409700 - 10/30/10 05:47 AM (13 years, 5 months ago) |
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It would allow me to give up some boring procedures and maybe even cultivate in open air...
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado 
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Re: antimicrobial plants myc friendly ? [Re: cubino]
#13409706 - 10/30/10 05:49 AM (13 years, 5 months ago) |
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Bacteria aren't a threat in open air. 
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You make it all possible. Doesn't it feel good?
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anonjon
Partially Right
Registered: 11/03/08
Posts: 6,322
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Re: antimicrobial plants myc friendly ? [Re: Doc_T]
#13409750 - 10/30/10 06:32 AM (13 years, 5 months ago) |
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Yeah if we could cultivaet in open air we could just put holes in the tubs instead of fooling with stupid polyfill. thats a good idea
the best additives are parsley sage rosemary and thyme
and milk, coca-cola, and pineapple juice
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The above post is fictional, hypothetical, or downright nonsensical.
Edited by anonjon (10/30/10 06:34 AM)
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: antimicrobial plants myc friendly ? [Re: anonjon]
#13409763 - 10/30/10 06:42 AM (13 years, 5 months ago) |
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Quote:
anonjon said:
the best additives are parsley sage rosemary and thyme
There's one more herb on my list.
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You make it all possible. Doesn't it feel good?
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andymc
cocoa beetles from zanzibar
Registered: 11/25/09
Posts: 2,395
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Re: antimicrobial plants myc friendly ? [Re: Doc_T]
#13409845 - 10/30/10 07:53 AM (13 years, 5 months ago) |
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Quote:
Doc_T said:
Bacteria aren't a threat in open air.
 Really Doc? I think I must be missing some context here.
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How I make spore prints
Trade List
My flow hood
If he asks me "Did you have a good time?" I'll say,
"Get the lights, Mr. Grim Reaper" -odds
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: antimicrobial plants myc friendly ? [Re: andymc]
#13409873 - 10/30/10 08:00 AM (13 years, 5 months ago) |
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Leave an agar plate open, see what grows on it.
Don't open and close it and parafilm it- open it and leave it.
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You make it all possible. Doesn't it feel good?
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andymc
cocoa beetles from zanzibar
Registered: 11/25/09
Posts: 2,395
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Re: antimicrobial plants myc friendly ? [Re: Doc_T]
#13409888 - 10/30/10 08:05 AM (13 years, 5 months ago) |
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Are you suggesting there are no airborne bacteria?
I'm quite sure spores are more numerous and/or would grow faster on exposed agar, so a better test would be to expose the agar and then cover it. Then you'll find out what landed on it in just the few seconds it was exposed.
I'd bet money on bacteria along with spores.
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How I make spore prints
Trade List
My flow hood
If he asks me "Did you have a good time?" I'll say,
"Get the lights, Mr. Grim Reaper" -odds
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: antimicrobial plants myc friendly ? [Re: andymc]
#13409914 - 10/30/10 08:15 AM (13 years, 5 months ago) |
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Quote:
andymc said: Are you suggesting there are no airborne bacteria?
How are bacteria like white men? They're ubiquitous, pernicious, and they can't jump.
Quote:
andymc said:
I'm quite sure spores are more numerous and/or would grow faster on exposed agar, so a better test would be to expose the agar and then cover it. Then you'll find out what landed on it in just the few seconds it was exposed.
That's got nothing to do with a bulk tub in fruiting conditions, which is the subject of this thread.
Even if bacteria were sneezed into the fc, they can't flourish in a proper fc.
Quote:
andymc said:
I'd bet money on bacteria along with spores.
I'd bet on my knowledge of microbiology. But I'd be stoked if you prove me wrong. 
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You make it all possible. Doesn't it feel good?
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andymc
cocoa beetles from zanzibar
Registered: 11/25/09
Posts: 2,395
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Re: antimicrobial plants myc friendly ? [Re: Doc_T]
#13409943 - 10/30/10 08:26 AM (13 years, 5 months ago) |
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Quote:
Doc_T said:
How are bacteria like white men? They're ubiquitous, pernicious, and they can't jump.
I realize this is a joke, but let's stipulate that bacteria need neither jumping ability nor wings to be carried on air currents along with all the other dust, spores, and crap floating around in a typical room...
A quick google of "airborne bacteria" turns up studies galore.
Quote:
Doc_T said:
That's got nothing to do with a bulk tub in fruiting conditions, which is the subject of this thread.
I guess there's the context I was missing.
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How I make spore prints
Trade List
My flow hood
If he asks me "Did you have a good time?" I'll say,
"Get the lights, Mr. Grim Reaper" -odds
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anonjon
Partially Right
Registered: 11/03/08
Posts: 6,322
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Re: antimicrobial plants myc friendly ? [Re: Doc_T]
#13409954 - 10/30/10 08:34 AM (13 years, 5 months ago) |
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Quote:
Doc_T said:
How are bacteria like white men? They're ubiquitous, pernicious, and they can't jump.
Thats a good one, u just come up with that?
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The above post is fictional, hypothetical, or downright nonsensical.
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: antimicrobial plants myc friendly ? [Re: andymc]
#13409982 - 10/30/10 08:45 AM (13 years, 5 months ago) |
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Quote:
andymc said:
let's stipulate that bacteria need neither jumping ability nor wings to be carried on air currents .
Can be carried but must be projected. Bacteria are not lifted by random air currents. (*)
And bacteria require liquid moisture, which means droplet range, about 3-5 ft.
Again, unless you're sneezing on your substrate bacteria aren't even going to be present other than those surface bacteria you'd find on anything.
Bacteria won't even flourish in a properly prepared bulk tub, there's not enough liquid, and too much air. And then there's the UV once you turn on the lights.
(*) endospores can be, but they're completely irrelevant to the thread.
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You make it all possible. Doesn't it feel good?
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curenado
73rd Man
Registered: 04/01/03
Posts: 2,603
Loc: North Central Arkansas
Last seen: 19 hours, 27 minutes
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Re: antimicrobial plants myc friendly ? [Re: Doc_T]
#13410019 - 10/30/10 09:00 AM (13 years, 5 months ago) |
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Gram Positive(+) Bac's die fast without moisture -
Gram Negative(-) Bac's liike e-coli are tougher and can last longer
without a host, but are less likely to apply in this sit.
Virus travels on air best...but typically pretty flimsy
Why don't you try just putting a slice of oinion in there? They are GREAT crud catchers...(on something, not in contact with your sub)
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Yours in the Natural State Land of Enchantment!
"The woods are lovely, dark and deep; but I have patches to keep, and jars to sterilize before I sleep...."
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ICdeadPeople
Fungi Fun Guy
Registered: 12/02/08
Posts: 566
Loc: sometimes MARS...
Last seen: 11 years, 5 months
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Re: antimicrobial plants myc friendly ? [Re: anonjon]
#13410181 - 10/30/10 09:55 AM (13 years, 5 months ago) |
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Quote:
anonjon said:
Yeah if we could cultivaet in open air we could just put holes in the tubs instead of fooling with stupid polyfill.
Shotgun FC, MSG (monotub/shotgun hybrid) no polyfill 
but i know what u mean 
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I'm ready to go on a GREAT adventure... Sitting here on the couch, eating my favorite snack 
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idunno
PinkWebBuffalo
Registered: 03/02/10
Posts: 1,087
Last seen: 12 years, 8 months
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Re: antimicrobial plants myc friendly ? [Re: curenado]
#13410203 - 10/30/10 10:06 AM (13 years, 5 months ago) |
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How does the onion work? Have read that onions and garlic are susposed to have anti microbial properties- Just curious
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The death of one man is a tragedy. The death of millions is a statistic.. Josef Stalin
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LeopardMan
Constantly changing
Registered: 09/21/09
Posts: 5,467
Loc: A tree house
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Re: antimicrobial plants myc friendly ? [Re: anonjon]
#13410810 - 10/30/10 02:42 PM (13 years, 5 months ago) |
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Quote:
anonjon said:
and milk, coca-cola, and pineapple juice
 
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You have to die a few times before you can really live.
-Charles Bukowski-
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cubino
Curious
Registered: 05/19/10
Posts: 18
Last seen: 13 years, 3 months
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Re: antimicrobial plants myc friendly ? [Re: LeopardMan]
#13415353 - 10/31/10 05:40 PM (13 years, 5 months ago) |
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How to have a "scientific" experiment ?
Maybe take some agar or substrate, PC it then put in 5-10 cups and add different herbs extracts and mycelium and then wait ...
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