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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Vermiculite

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Amazon BasinRepresentative

Registered: 07/12/02
Posts: 86
Loc: A small planet in the esc...
Last seen: 13 years, 9 months
What Happened!
    #1339807 - 02/27/03 09:01 AM (15 years, 3 months ago)

So a friend of a friend inoculated 12 1/2 pint jars of BRF and Vermiculite with a syringe from a reputable vendor. The jars were each placed in a PC at 12-15 psi for one hour.

Before inoculation, the work area was cleaned using Alcohol and mucho Lysol Spray both in the air and on surfaces. To get a good shake in the syringe, my friend sucked in a tiny bit of air and then shook the sucker like crazy. The syringe was sterilized with a regular butane lighter between every two jars.

After inoculation, the tape was retuned to the top of the lids, and the jars were put in a box on top of a closet shelf for 8 days.

On the eighth day, the jars were taken down and examined. Without exception, there was green mold at each of the 4 inoculation points in all 12 jars.

This being my friend of a friend's third attempt (both the others being totally successful) she had never seen anything like this.

The question is what could have caused such uniform contamination? The three things that come to mind are

1. Bad syringe
2. When the air was sucked in to shake with, something got sucked in too and ruined the whole batch.
3. Some freaky contamination problem in the storage box in the closet.

Are any of these likely/probable, and if not, what are other factors that could cause such uniform contamination problems, so I can try to limit all of those factors?

Everybody knows what is going wrong with the world,
I don't even know what's going on . . . in myself.

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Registered: 06/13/01
Posts: 67
Loc: Thither
Last seen: 14 years, 5 months
Re: What Happened! [Re: OKKuato]
    #1339817 - 02/27/03 09:04 AM (15 years, 3 months ago)

I'd say the syringe was contaminated. I'd ask for your money back.

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mind xplorer

Registered: 10/19/02
Posts: 85
Last seen: 10 years, 1 month
Re: What Happened! [Re: Slipstream]
    #1340328 - 02/27/03 11:48 AM (15 years, 3 months ago)

Happened to me.

I had 2 different syringes each from the same reputable vendor. One strain colonized  the other strain left nasty smelling mold cakes! :tongue:

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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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