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Shitsheriff
Strangler


Registered: 09/29/10
Posts: 12
Loc: Alberta Canada
Last seen: 13 years, 2 months
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Making MYA agar right now and i have a big question
#13327333 - 10/12/10 07:08 PM (13 years, 4 months ago) |
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kay guys, made 1L of MYA agar.. actually im making it.
Right now i got 4 1/2 pint jars loaded in my PC'er. The jars are 90% full with the lids seal down but only put on LOOSELEY
Am i going to blow the place up, will the AGAR bubble over and out into the PC water.. Any tips guys thanks..
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Shitsheriff
Strangler


Registered: 09/29/10
Posts: 12
Loc: Alberta Canada
Last seen: 13 years, 2 months
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Re: Making MYA agar right now and i have a big question [Re: Shitsheriff]
#13327865 - 10/12/10 09:03 PM (13 years, 4 months ago) |
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Jeeez thanks guys
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: Making MYA agar right now and i have a big question [Re: Shitsheriff]
#13327913 - 10/12/10 09:11 PM (13 years, 4 months ago) |
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I would highly suggest using an old Whisky or other glass liquor bottle with a poly-fill/tyvek breather in the lid. Check out http://www.mushroomvideos.com/Agar-Petri-Dishes to see exactly what I am talking about.
This method will allow the container to breath, and if you let it cool slowly you should not have boil over.
P.S Sometimes it takes time to get a response try not to bump your thread unless it has been 24 hours since your last post. As well RR's videos are a big help
Edited by EvilMushroom666 (10/12/10 09:14 PM)
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Shitsheriff
Strangler


Registered: 09/29/10
Posts: 12
Loc: Alberta Canada
Last seen: 13 years, 2 months
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Re: Making MYA agar right now and i have a big question [Re: EvilMushroom666]
#13327937 - 10/12/10 09:14 PM (13 years, 4 months ago) |
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I read your tek about the wiskey bottle as the jars were sitting in the PC. I was rushing around to find something small, like a crown royal bottle as my PC isint very large.
I think everything worked out alright, next time im hoping for such a quick response ill use IRC. Thanks alot for your helpful guides
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Peterthinks
(Caulking) gun for hire

Registered: 11/10/04
Posts: 2,379
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Re: Making MYA agar right now and i have a big question [Re: EvilMushroom666]
#13327949 - 10/12/10 09:16 PM (13 years, 4 months ago) |
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Your jars will not blow up, they will seal themselves with a partial vacuum as they cool. I store extra agar like this. you can keep it a couple years till you need it.
Breaking the seal is a bitch (use a sterile flamed knife tip) but if you open them somewhere clean like a glove box or in front of a flow hood no harm done.
Congrats! you have just canned your agar!!
-------------------- Give a man a fire and he will be warm for the rest of the night. Set a man on fire and he will be warm for the rest of his life. NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS? Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: Making MYA agar right now and i have a big question [Re: Shitsheriff]
#13328003 - 10/12/10 09:25 PM (13 years, 4 months ago) |
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No worries, thanks for your addition to my Grow supplies in Canada thread. Welcome to the Shroomery. Let me be the first to lay +5 on you. You will soon learn that there are 1 million ways to skin a cat, but some things are standard procedure. I personally would not create vacuum sealed agar unless I had a flowhood to work in front of.
Do not let this next bit scare you off, but be careful pouring agar in your glove box. Make sure your GB is big enough for the batch you are doing, take your time preparing, let your agar cool till you can handle the bottle/jar, and try to make your pours quick and clean. Never hold parts of your hand/fingers over the petri's.
I had my last batch of GB agar pours fail because of the reasons above minus placing my hand/fingers over the dish's. Losing 19 out of 20 dish's is humbling and makes you realize how careful you must be.
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Peterthinks
(Caulking) gun for hire

Registered: 11/10/04
Posts: 2,379
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Re: Making MYA agar right now and i have a big question [Re: Peterthinks]
#13328010 - 10/12/10 09:26 PM (13 years, 4 months ago) |
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When it is done just shut off the heat let it cool on the element slowly. when the safety goes down (silver knob on presto cooker) or whatever indicator says it's ok to open let it cool till you can hold it and it's time to pour.
I find a syringe is great, suck up 5 cc in a sterile syringe and put it in a dish. your dish size may be different but put in whatever it takes to make 2 millimeters of agar in it.
Repeat as needed, stack.
Let cool and use.
If you don't have Parafilm use sections of plastic wrap, cut one inch chunks off a roll and stretch it around.
 PM me if you have problems, when I sober up I'll reply....
-------------------- Give a man a fire and he will be warm for the rest of the night. Set a man on fire and he will be warm for the rest of his life. NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS? Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.
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Peterthinks
(Caulking) gun for hire

Registered: 11/10/04
Posts: 2,379
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Re: Making MYA agar right now and i have a big question [Re: Peterthinks]
#13328036 - 10/12/10 09:29 PM (13 years, 4 months ago) |
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Great pour bottles! the plastic screw top lid can be PCd, get the 250 ml one it'll fit in your PC.
-------------------- Give a man a fire and he will be warm for the rest of the night. Set a man on fire and he will be warm for the rest of his life. NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS? Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: Making MYA agar right now and i have a big question [Re: Peterthinks]
#13328069 - 10/12/10 09:34 PM (13 years, 4 months ago) |
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Peter has the right idea. I have used 250ml bottles and they work fine. Mix up 100ml of agar (4-5 dish's worth) and place it in the bottle. You should never fill an agar bottle with a filtered lid more then halfway or it will most likely boil over. When I use a 750ml bottle I can fit 500ML of agar inside without issues.
As well place a bit more water in your PC when doing agar. This will help reduce boil over as well.
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Peterthinks
(Caulking) gun for hire

Registered: 11/10/04
Posts: 2,379
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Re: Making MYA agar right now and i have a big question [Re: EvilMushroom666]
#13328117 - 10/12/10 09:39 PM (13 years, 4 months ago) |
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Not to confuse the issue but sometimes I spoon chilled agar into dishes and then PC the dishes with the tops on.
PP plastic or glass/Pyrex dishes only.
Ya heat it slow and cool it slow, all good.
I built little racks to keep them level.
Just stick to the normal way, then play around.
-------------------- Give a man a fire and he will be warm for the rest of the night. Set a man on fire and he will be warm for the rest of his life. NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS? Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.
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Peterthinks
(Caulking) gun for hire

Registered: 11/10/04
Posts: 2,379
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Re: Making MYA agar right now and i have a big question [Re: EvilMushroom666]
#13328157 - 10/12/10 09:45 PM (13 years, 4 months ago) |
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Quote:
EvilMushroom666 said: Peter has the right idea. I have used 250ml bottles and they work fine. Mix up 100ml of agar (4-5 dish's worth) and place it in the bottle. You should never fill an agar bottle with a filtered lid more then halfway or it will most likely boil over. When I use a 750ml bottle I can fit 500ML of agar inside without issues.
As well place a bit more water in your PC when doing agar. This will help reduce boil over as well.
Yes more water, 4 inches I use and let it cool on the element.
Slooooooowwwwwwwww!
Or it will boil and fuck you, not that cool "My girlfriend stuck her finger up my ass while she blew me!" More like that "I got thrown in the drunk tank with some dude named BUBBA and he fucked me!" That kind of Fucked...I really should get off line now....
7 beer post sorry, still kinda valid, I stand by it.
-------------------- Give a man a fire and he will be warm for the rest of the night. Set a man on fire and he will be warm for the rest of his life. NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS? Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: Making MYA agar right now and i have a big question [Re: Peterthinks]
#13328198 - 10/12/10 09:51 PM (13 years, 4 months ago) |
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Atta boy! lol Sound a bit tipsy there. Iv gotten fucked by agar a few times. Trying to pour slants twice iv had that ah shit moment. I then invested in proper PP cultubes and all is well.
As mentioned above also had a HUGE contam problem that ruined an entire batch. Rushing + To big of a batch in to small of a glove box = Fail
Make sure you set aside enough time to complete your task. Also make sure you will not be disturbed or be called away from your project...Im rambling, that other Canadian is drunk, and this Canadian is stoned
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Peterthinks
(Caulking) gun for hire

Registered: 11/10/04
Posts: 2,379
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Re: Making MYA agar right now and i have a big question [Re: EvilMushroom666]
#13328256 - 10/12/10 10:01 PM (13 years, 4 months ago) |
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LOL Evilmushroom666 send me a PM we can do some pouring!!!!
 You can be covered in shit, just keep your hands behind the dishes, it'll be OK!
P.S. Bring BEER!!!
-------------------- Give a man a fire and he will be warm for the rest of the night. Set a man on fire and he will be warm for the rest of his life. NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS? Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: Making MYA agar right now and i have a big question [Re: Peterthinks]
#13328267 - 10/12/10 10:02 PM (13 years, 4 months ago) |
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Oh how I wish we lived in the same province! lol
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Base Icks



Registered: 03/19/10
Posts: 6,191
Loc: Shroomshire
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Re: Making MYA agar right now and i have a big question [Re: EvilMushroom666]
#13328415 - 10/12/10 10:32 PM (13 years, 4 months ago) |
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I use 4 beer bottles in my 6L pc.

Tyvek and foil for the lid. Held on by rubber bands.
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