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Enema Bag Jones

Registered: 10/17/00
Posts: 1,091
Loc: Warm, Moist and Dark
Lack of foresight, suggestions anyone?
    #1328823 - 02/23/03 01:56 PM (13 years, 9 months ago)

This guy I know streaked for isolated colonies on an SDA plate. The resulting colonies were then transplanted into seperate plates. Now he has 5 plates of mycelium growing from 1 spore, 5 plates growing from another spore, and a plate with many different mycelia from different spores growing together (the initial plate).

The guy knows they won't fruit as a haploid organism. What the hell should he do with those 10 (not fully colonized)plates? He really does not want to waste them.


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Man thinks. God laughs. - Jewish Proverb


Edited by no-tone (02/23/03 02:00 PM)


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InvisibleG a n j a
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Re: Lack of foresight, suggestions anyone? [Re: no-tone]
    #1328840 - 02/23/03 02:04 PM (13 years, 9 months ago)

how did you manage one spore?are you sure of that?


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OfflineDEA OFFICER
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Re: Lack of foresight, suggestions anyone? [Re: no-tone]
    #1328842 - 02/23/03 02:05 PM (13 years, 9 months ago)

Spores usually clump togather so chances are those ten plates are dikaryotic.

... I think :crazy: 


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Enema Bag Jones

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Re: Lack of foresight, suggestions anyone? [Re: DEA OFFICER]
    #1328859 - 02/23/03 02:16 PM (13 years, 9 months ago)

He is positive that those colonies were each from one spore. Thats the idea of streaking for isolated colonies.

For the fungi to fruit, does the mycelium have to be +/-?
Or can it be -/- or +/+?
He was thinking of cutting half from each plate and transfering to the other.


--------------------
Man thinks. God laughs. - Jewish Proverb


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OfflineDEA OFFICER
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Re: Lack of foresight, suggestions anyone? [Re: no-tone]
    #1329436 - 02/23/03 07:20 PM (13 years, 9 months ago)

I would imagine that if getting isolated colonies from a single spore were that easy we would have plenty of hybrids around. If he is certain it is haploid then he ought to try mating it with another strain or even species.


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Offlinezeronio
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Re: Lack of foresight, suggestions anyone? [Re: no-tone]
    #1330108 - 02/24/03 01:58 AM (13 years, 9 months ago)

Quote:

He is positive that those colonies were each from one spore. Thats the idea of streaking for isolated colonies.




There is no way that he got a monokariotic mycellium if he streaked spores with inoculation loop and even if he did it somehow he cannot be positive about it. The right procedure to do that would be to use a spore dillution technique and inoculate hundreds of petris and hope to get few monokarions.


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Enema Bag Jones

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Re: Lack of foresight, suggestions anyone? [Re: zeronio]
    #1330347 - 02/24/03 04:32 AM (13 years, 9 months ago)

This guy plans on doing a massive amount of substrate. He just doesn't want to innoculate and end up with a significant amount of useless colonized substrate. Should he just proceed with the innoculation? Thanks for the help.


--------------------
Man thinks. God laughs. - Jewish Proverb


Edited by no-tone (02/24/03 04:34 AM)


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InvisibleG a n j a
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Re: Lack of foresight, suggestions anyone? [Re: no-tone]
    #1330372 - 02/24/03 04:43 AM (13 years, 9 months ago)

Yes.Or wait a bit do a test batch with cakes and choose the best culture i suppose.I would put ?10 on none of those cultures are haploid.


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OfflineRaadt
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Re: Lack of foresight, suggestions anyone? [Re: no-tone]
    #1330849 - 02/24/03 08:11 AM (13 years, 9 months ago)

There is almost no way that with streaking you got a monokaryon. Streaking allows different dikaryotic growths within the same plate, in order to allow the cultivator to select the strongest growths. I think you misunderstood, it does not allow someone to select strong monokaryon growth.


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Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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Offlinehsalf
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Re: Lack of foresight, suggestions anyone? [Re: no-tone]
    #1331923 - 02/24/03 03:33 PM (13 years, 9 months ago)

In agreement w/zeronio, ganja, & raadt.
If doing "massive" amounts of substrate you'd probablly want to run test batches. Though not because you believe you have haploid myc. In the end, the small amount of time spent will insure uniform flushes and increased yeilds.


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Offlinezeronio
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Re: Lack of foresight, suggestions anyone? [Re: no-tone]
    #1332537 - 02/24/03 07:39 PM (13 years, 9 months ago)

It's very likely that he'd get fruits anyway, but like other said it would be better to make a small casing first and clone the best looking fruit.


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Enema Bag Jones

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Re: Lack of foresight, suggestions anyone? [Re: Raadt]
    #1333830 - 02/25/03 09:27 AM (13 years, 9 months ago)

Quote:

Streaking allows different dikaryotic growths within the same plate, in order to allow the cultivator to select the strongest growths.




I'm still a little confused about this.

Where is this dikaryotic growth coming from? Should he have waited for the mycelia to fuse (producing a diploid) before taking tissue samples and transferring them to other plates?


--------------------
Man thinks. God laughs. - Jewish Proverb


Edited by no-tone (02/25/03 09:28 AM)


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Offlinezeronio
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Re: Lack of foresight, suggestions anyone? [Re: no-tone]
    #1335916 - 02/25/03 08:11 PM (13 years, 9 months ago)

The spores usually clump together and the monokariotic mycellium mates immediatly as soon as it germinates. It's very likely that the first growth he saw was already dikaryotic. He doesn't have to wait for the mycelia to fuse.
Just tell him to transfer it to grain and grow the mushrooms!  :grin: 


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Enema Bag Jones

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Re: Lack of foresight, suggestions anyone? [Re: zeronio]
    #1336405 - 02/26/03 01:56 AM (13 years, 9 months ago)

Great! Thanks everyone, I'll let him know to do a test batch soon :]


--------------------
Man thinks. God laughs. - Jewish Proverb


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Enema Bag Jones

Registered: 10/17/00
Posts: 1,091
Loc: Warm, Moist and Dark
Re: Lack of foresight, suggestions anyone? [Re: zeronio]
    #1341817 - 02/28/03 03:09 AM (13 years, 9 months ago)

So you all think that the clump of spores that germinated had the right mating types to produce fruit?


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Man thinks. God laughs. - Jewish Proverb


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InvisibleG a n j a
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Re: Lack of foresight, suggestions anyone? [Re: no-tone]
    #1341914 - 02/28/03 04:34 AM (13 years, 9 months ago)

yep


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Enema Bag Jones

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Re: Lack of foresight, suggestions anyone? [Re: G a n j a]
    #1341953 - 02/28/03 05:02 AM (13 years, 9 months ago)

Great, he thanks you all.


--------------------
Man thinks. God laughs. - Jewish Proverb


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