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afoaf
CEO DBK?


Registered: 11/08/02
Posts: 32,665
Loc: Ripple's Heart
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spore germination...some grains better than others?
#1323715 - 02/21/03 02:36 AM (20 years, 9 months ago) |
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afoaf of afoaf knocked up a couple different types of grains...all prepared in the same fashion and innoculated with the same set of syringes from the same print, sometimes splitting syringes across jars.
dude noticed that the millet jars began germination in 36 hours, while only one out of 8 wheat jars are even showing slight signs of germination after 4 days.
(note: all of which have been sitting in the same incubator set to the low 80's. )
do folks have any opinions or fact based observations regarding whether or not some grains foster germination better than others?
feel free to bump this back to cult if need be.
thanks in advance for your insight.
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deanofmean
mycophagous

Registered: 12/06/02
Posts: 2,017
Loc: PNW
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Re: spore germination...some grains better than others? [Re: afoaf]
#1323754 - 02/21/03 02:52 AM (20 years, 9 months ago) |
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i was told wheat is a poor substrate, because it is high in protein .
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afoaf
CEO DBK?


Registered: 11/08/02
Posts: 32,665
Loc: Ripple's Heart
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Re: spore germination...some grains better than others? [Re: deanofmean]
#1323766 - 02/21/03 02:58 AM (20 years, 9 months ago) |
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Top of page 43 in TMC:
WHEAT: Works equally well as rye for spawn making and fruitbody production.
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Peaceful_Nomad
On the Path ofthe Feather


Registered: 06/23/02
Posts: 447
Loc: Sometimes Kansas - Maybe ...
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Re: spore germination...some grains better than others? [Re: afoaf]
#1323810 - 02/21/03 03:31 AM (20 years, 9 months ago) |
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Red wheat berries work better than rye. You only have to soak them for maximum hydration and the PC'ng will make the grains absorb any residual H2O without adding additional water.
Wheat is cheaper than rye in my area.
Peace to Everyone,
Peaceful Nomad
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deanofmean
mycophagous

Registered: 12/06/02
Posts: 2,017
Loc: PNW
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Re: spore germination...some grains better than others? [Re: afoaf]
#1323811 - 02/21/03 03:31 AM (20 years, 9 months ago) |
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in the table below that he lists perennial wheat aka derum or winter wheat, which is used for animal feed and would have a protein content of 16% or less (i think). the kind of wheat that is sold in a grocery store for bread making is 26-32% .
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 1 month, 12 hours
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Re: spore germination...some grains better than others? [Re: afoaf]
#1323879 - 02/21/03 04:01 AM (20 years, 9 months ago) |
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your friend may have had a perfect water ratio in his millet but not in the wheat. if he tried again he may find the wheat is better if the ratios change.
wheat is cheaper for me too, rye isnt grown here much. pet shops have it very cheap. i had no success with it though.
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afoaf
CEO DBK?


Registered: 11/08/02
Posts: 32,665
Loc: Ripple's Heart
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Re: spore germination...some grains better than others? [Re: blackout]
#1324035 - 02/21/03 05:28 AM (20 years, 9 months ago) |
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all grains were prepared by soaking for 36-48 hours, rinsing, draining and pcing without additional water supplements.
wouldn't it be safe to assume that when prepared this way, that all the grains would achieve optimal water content?
deanofmean, thanks, I think you are correct, the soft wheat that I am using is not the same as the harder, winter wheat. but even so, would that affect initial germination of the spores?
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NeonBlack
enthusiast
Registered: 11/23/00
Posts: 331
Last seen: 14 years, 3 months
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Re: spore germination...some grains better than others? [Re: deanofmean]
#1324044 - 02/21/03 05:34 AM (20 years, 9 months ago) |
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Most health food stores around here stock hard red winter wheat berries .. You can get it bulk for pretty cheap.
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debianlinux
Myconerd - DBK



Registered: 12/09/02
Posts: 8,334
Loc: Over There
Last seen: 5 months, 8 days
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Re: spore germination...some grains better than others? [Re: afoaf]
#1324130 - 02/21/03 06:22 AM (20 years, 9 months ago) |
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Quote:
all grains were prepared by soaking for 36-48 hours, rinsing, draining and pcing without additional water supplements.
wouldn't it be safe to assume that when prepared this way, that all the grains would achieve optimal water content?
no... i met this bum who learned this the hard way when she started with grains. wheat/rye needs an optimal water content that is specific to the specific grain your using. IOW, my rye berries may very well require a different amount of water than yours. the only way to determine this is to make batches with varying water amounts and note the best results and use THAT amount of water from that point on with that source/brand of rye berries. millet tends to have the unique advantage of simply needing to be presaoked like you were asking. this is why that bum moved away from rye and used millet from that point on. (I'd like to point out that I believe the water ratio is pretty standard for rye but the different ryes have different amounts of inherent water that could only be determined by weighing the grain, baking all the water out and re-weighing to note the difference, in my opinion it's all too much of a pain in the ass when millet is so damn easy)
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afoaf
CEO DBK?


Registered: 11/08/02
Posts: 32,665
Loc: Ripple's Heart
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Re: spore germination...some grains better than others? [Re: debianlinux]
#1324398 - 02/21/03 08:34 AM (20 years, 9 months ago) |
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even when soaked for days and days, the grain still may not absorb the maximum amount of water that it could absorb...?
or is the point that maximum water capacity is not the same as optimal water content?
even if you assumed that both sets of jars had the optimal (and/or maximum) water content, would one grain be better than the other for germination?
or, in simpler terms, to genericize the topic of the thread, are some surfaces or substrates better for germination than others. and if there is such thing as a superior medium for spore germination, what about it makes it so...
is it strictly nutritional value or some other combination of traits and qualities?
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NeonBlack
enthusiast
Registered: 11/23/00
Posts: 331
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Re: spore germination...some grains better than others? [Re: afoaf]
#1324470 - 02/21/03 09:01 AM (20 years, 9 months ago) |
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I don't think that maximum water content is necessarily the same as optimal water content. It does make logical sense that some substrates would be better than others for spore germination. I have a hunch that water content has more to do with the actual initial germination of the spores. After that, it seems like a combination of nutritional content and water content would come into play. So, what I'm guessing is that a medium that is optimal for spore germination may not be as good for prolonged growth. But, then again, I might be full of shit.
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debianlinux
Myconerd - DBK



Registered: 12/09/02
Posts: 8,334
Loc: Over There
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Re: spore germination...some grains better than others? [Re: NeonBlack]
#1324551 - 02/21/03 09:32 AM (20 years, 9 months ago) |
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neonblack hit that shit right on the head. (saved me some typing) i would definitely contend that max water content does not equal optimal water content. water and a trace amount of nutrient is all that is really required for germination so the grain is realtively unimportant at that stage. water/grain ratio plays a large role in colonization speed which you want to optimize both to speed up time till harvest but more importantly to outrun competitor bacteria.
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Peace_Patrol
Rambling hippie
Registered: 04/22/02
Posts: 350
Loc: Electric Neverending
Last seen: 19 years, 3 months
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Re: spore germination...some grains better than others? [Re: afoaf]
#1325721 - 02/22/03 12:33 AM (20 years, 9 months ago) |
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I've had germination on wheat within 36 hours from spore syringe.
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