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 Baby_Hitler
Errorist
Registered: 03/06/02
Posts: 27,652
Loc: To the limit!
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 Starting Shitake from syringe? 
#1321898 - 02/20/03 09:25 AM (21 years, 1 month ago) |
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I have a syringe of shitake mycelium. What medium should I use to get it started?
You think popcorn would work? Maybe start on popcorn, then spawn to sawdust, or wooden dowels?
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 G a n j a
Pictish and proud
Registered: 12/03/02
Posts: 7,860
Loc: Zone ate
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Re: Starting Shitake from syringe? [Re: Baby_Hitler]
#1322010 - 02/20/03 10:01 AM (21 years, 1 month ago) |
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Im doing some dowels at the moment have you seen my grow log?
Any tips or comments will be good.
If i had a mycelium syringe i would inoculate grain with half and dowels with the other.Then put the grain to sawdust blocks or bags and the dowels to logs if you have outdoor space.
I only started dowels  so i better get some millet going as well cos some one just dropped of more oak than i have dowels for so ill need them all.
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Baby_Hitler
Errorist
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Re: Starting Shitake from syringe? [Re: G a n j a]
#1322049 - 02/20/03 10:22 AM (21 years, 1 month ago) |
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>>>Im doing some dowels at the moment have you seen my grow log?<<<
No, where is it?
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r05c03
The Slug Scourge
Registered: 01/06/02
Posts: 383
Loc: Indiana, US
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Re: Starting Shitake from syringe? [Re: Baby_Hitler]
#1322093 - 02/20/03 10:38 AM (21 years, 1 month ago) |
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Inoculate several jarsof birseed, grass seed, or rye, or your other favorite grain spawn. if you have sterile conditions inoculate some petri plates too. Then you can use the spawm to do sawdust blocks, or dowels or whatever. You can really stretch your syringe that way.
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G a n j a
Pictish and proud
Registered: 12/03/02
Posts: 7,860
Loc: Zone ate
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Re: Starting Shitake from syringe? [Re: Baby_Hitler]
#1322130 - 02/20/03 10:58 AM (21 years, 1 month ago) |
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Baby_Hitler
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Re: Starting Shitake from syringe? [Re: r05c03]
#1322287 - 02/20/03 12:06 PM (21 years, 1 month ago) |
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I think I'll try it on popcorn first, then start some peroxidated agar plates, and sawdust/dowels with that.
I'll be using Potato (flakes) Fructose Agar.
I found this in the health food section at the local grocery store:
Frutose is a monoglyceride like dextrose, so it should work.
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MushMushi
Registered: 08/23/02
Posts: 480
Loc: Canada
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Re: Starting Shitake from syringe? [Re: Baby_Hitler]
#1322349 - 02/20/03 12:31 PM (21 years, 1 month ago) |
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Wouldn't it be easier to simply cook a few patatoes?
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Baby_Hitler
Errorist
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Re: Starting Shitake from syringe? [Re: MushMushi]
#1322407 - 02/20/03 01:00 PM (21 years, 1 month ago) |
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I don't plan on PCing the agar, so I'm using flakes since they don't seem to contain live peroxidase which would break down the peroxide.
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Hongosmeester
old sourdough
Registered: 12/12/02
Posts: 246
Loc: East Bay (EAST BAY GREASE...
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Re: Starting Shitake from syringe? [Re: r05c03]
#1322513 - 02/20/03 01:41 PM (21 years, 1 month ago) |
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Quote:
Inoculate several jars of birdseed, grass seed, or rye, or your other favorite grain spawn. if you have sterile conditions inoculate some petri plates too. Then you can use the spawm to do sawdust blocks, or dowels or whatever. You can really stretch your syringe that way.
I just got 100 dowels from Fungi Perfecti and want to know if I have to soak the logs can I innoculate bags of sawdust with some?. and for how long . How can I maximize the output - sawdust, spawn, logs etc . what can i use to seal the logs?
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r05c03
The Slug Scourge
Registered: 01/06/02
Posts: 383
Loc: Indiana, US
Last seen: 19 years, 10 days
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Re: Starting Shitake from syringe? [Re: Hongosmeester]
#1323537 - 02/20/03 10:58 PM (21 years, 1 month ago) |
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I would go ahead and sterilize at least one quart jar of grain and drop a dowel in there. It should colonize and you can use the colonized grain to inoculate more grain jars significantly stretching your dowels. As far as how many to put in a log I would put 25 to 50 dowels per 4-5 ft long log section. The log should be betweent 4-6 inches in diameter
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Raadt
nicht
Registered: 06/07/02
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Loc: azurescending
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Re: Starting Shitake from syringe? [Re: r05c03]
#1324110 - 02/21/03 06:14 AM (21 years, 1 month ago) |
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i'd use 3-4 to ensure colonization, shiitake isn't very fast.
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Raadt
-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--
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Baby_Hitler
Errorist
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Re: Starting Shitake from syringe? [Re: Raadt]
#1329012 - 02/23/03 01:38 PM (21 years, 1 month ago) |
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I put a 4-5 drops of mycelium water on some sterileized grey cardboard disks last night.
We'll see how they do.
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