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OfflineMaharishi_2_U
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My Agar Work
    #13209847 - 09/17/10 02:21 PM (13 years, 4 months ago)

I am trying to get a few good isolates and clones going on Agar.
I am using MEA 12g to 300ml water + 4 ml h2o2

I have two samples, the one with the number 12 on it I would like some expert advice as to which section you would xfer....
The other is my cloning attempt uno :smile:
So what do you think, using the 12 as a reference, which section should i use, imagine the sample as a clock.
:smile:
TIA


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InvisibleeLShaMukO
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Re: My Agar Work [Re: Maharishi_2_U]
    #13209970 - 09/17/10 02:52 PM (13 years, 4 months ago)

i see nice rizo at 10 and 11


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OfflineXiC_clone
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Re: My Agar Work [Re: Maharishi_2_U]
    #13210010 - 09/17/10 03:07 PM (13 years, 4 months ago)



:highfive: Looking awesome


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Invisiblehiggledy-piggledy

Registered: 08/24/10
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Re: My Agar Work [Re: eLShaMukO]
    #13210061 - 09/17/10 03:18 PM (13 years, 4 months ago)

dont know much about agar yet but im watching and learning

Quote:

eLShaMukO said:
i see nice rizo at 10 and 11



what he said

:goodluck:


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OfflineMaharishi_2_U
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Re: My Agar Work [Re: higgledy-piggledy]
    #13210396 - 09/17/10 04:22 PM (13 years, 4 months ago)

Thank you :smile:
Xfers first thing tomorrow, saturdays are myco daze round here :wink:


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Invisiblecyantific
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Re: My Agar Work [Re: Maharishi_2_U] * 1
    #13210448 - 09/17/10 04:34 PM (13 years, 4 months ago)

lmao your dishes are pretty much like mine cept i use crockery ... i thought i was the only ghetto-plate style agarist around here lol ... just make sure you get the utmost tips of those rhizos and nothing else so not to cloud the gene pool negligently ... will save you time in the end ...

ah shit man ur dishes aint ghetto thats something showing through ... thought those were designs on dinner plates lol


Edited by cyantific (09/17/10 04:38 PM)


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OfflineMaharishi_2_U
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Re: My Agar Work [Re: cyantific]
    #13210602 - 09/17/10 05:13 PM (13 years, 4 months ago)

lol no, that is my holiday table cloth lol.... i use poly petris....
i am just leanrin :smile:
thank u for the tip though mang :smile:


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Invisibletruskool
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Re: My Agar Work [Re: Maharishi_2_U]
    #13210815 - 09/17/10 06:02 PM (13 years, 4 months ago)

Id personally use the 1-3 just cause its faster growth and you still have time to sector for rhizo's, but 10-11 is nice growth


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OfflineMad_Hatter2004
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Re: My Agar Work [Re: truskool]
    #13210824 - 09/17/10 06:04 PM (13 years, 4 months ago)

I'd make some transfers from all three sectors transferring each sector to it's own respective petri dish of agar and then see what kind of growth you get on each plate and make more transfers from the best looking sectors of each plate.

Can't wait till I get into working with agar!


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OfflineeVenom
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Re: My Agar Work [Re: truskool]
    #13210923 - 09/17/10 06:26 PM (13 years, 4 months ago)

Quote:

truskool said:
Id personally use the 1-3 just cause its faster growth and you still have time to sector for rhizo's, but 10-11 is nice growth



:whathesaid:


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eVenom

"Some of us like a nice buzz, while others want to climb a screaming volcano naked to meet god."
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OfflineMaharishi_2_U
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Re: My Agar Work [Re: eVenom]
    #13211786 - 09/17/10 09:44 PM (13 years, 4 months ago)

Will listen to the experts then!!!
MH I hope you can roll along with this thread and learn by watchin a noob roll.
More info....
The peroxided agar does cause a stall out on the myc growth for sure.  I do my work in a still box, and still ( lol no pun intended ) had contams.
I did BOtH of thes transfers in very clean, yet open air......
So I figure from here on out until my FH is done it is MS -Milo - fruit - clone H2O2 agar -bulk
:smile:
very excited to finnaly be "getting" some of this!!! :smile:


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OfflineeVenom
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Re: My Agar Work [Re: Maharishi_2_U]
    #13211811 - 09/17/10 09:50 PM (13 years, 4 months ago)

what kind of contamination did you have? fungal or bacteriological?

peroxide will not solve any of your problems!


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eVenom

"Some of us like a nice buzz, while others want to climb a screaming volcano naked to meet god."
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InvisiblePeterthinks
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Re: My Agar Work [Re: eVenom]
    #13211862 - 09/17/10 09:59 PM (13 years, 4 months ago)

10 and 11, do 1:45 too, I got a feeling.

I ....my wife just bitched me out for like 20 minutes just now, I totally forgot what I was going to say.



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Edited by Peterthinks (09/17/10 10:10 PM)


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InvisibleJavadog
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Re: My Agar Work [Re: Peterthinks]
    #13211916 - 09/17/10 10:08 PM (13 years, 4 months ago)

The growth in 10-12 looks strong.

Good luck!

P.S. For anyone who read the thread, I now have recovered
all but two of my (what turned out to be) ten bacteria
infected syringes.  Each time I had to do at least two
transfers for each strain.  Now I have grain jars with
wedges and am testing a wedge-based LC this evening. 
Agar rocks!


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InvisiblePeterthinks
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Re: My Agar Work [Re: Javadog]
    #13211949 - 09/17/10 10:15 PM (13 years, 4 months ago)

Quote:

Javadog said:
The growth in 10-12 looks strong.

Good luck!

P.S. For anyone who read the thread, I now have recovered
all but two of my (what turned out to be) ten bacteria
infected syringes.  Each time I had to do at least two
transfers for each strain.  Now I have grain jars with
wedges and am testing a wedge-based LC this evening. 
Agar rocks!




You are my hero.

I have some old syringes to sort out...I see light at the end of the tunnel.
I am thinking of sucking some blank LC solution into them and making LC then going to agar to sector it.


--------------------
Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS?
Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.


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OfflineMad_Hatter2004
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Re: My Agar Work [Re: Maharishi_2_U]
    #13212146 - 09/17/10 10:48 PM (13 years, 4 months ago)

Quote:

boredcertified said:
Will listen to the experts then!!!
MH I hope you can roll along with this thread and learn by watchin a noob roll.




I've definitely done some reading on agar and strain isolation and have a pretty good idea about the whole process...but still,nothing beats hands on experience :thumbup:


Quote:

boredcertified said:
I do my work in a still box, and still ( lol no pun intended ) had contams.




That's why I am waiting until my lease is up and I get another place before I start agar work.At the moment the place I am in is too small and I have no where to place a flowhood.It's better for me to be patient and just wait for that moment in time as agar work in glove box.still air box would be very cramped,as well as g2g or a2g transfers.


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InvisibleJavadog
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Re: My Agar Work [Re: Peterthinks]
    #13212396 - 09/17/10 11:40 PM (13 years, 4 months ago)

Quote:

Peterthinks said:
Quote:

Javadog said:
The growth in 10-12 looks strong.

Good luck!

P.S. For anyone who read the thread, I now have recovered
all but two of my (what turned out to be) ten bacteria
infected syringes.  Each time I had to do at least two
transfers for each strain.  Now I have grain jars with
wedges and am testing a wedge-based LC this evening. 
Agar rocks!




You are my hero.

I have some old syringes to sort out...I see light at the end of the tunnel.
I am thinking of sucking some blank LC solution into them and making LC then going to agar to sector it.




Thanks man.

Starting from LC can work.

Here is a photo of one of my dishes:


(clearly, I need to let my agar cool more)

This is Mexicube and was one of my dirtiest looking dishes
when I started.

Only Koh Samui has yet to make anything usable to work with.

Onward and upward,

JD


--------------------
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Myco-tek.org


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OfflineDoctopus
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Re: My Agar Work [Re: Javadog]
    #13212450 - 09/18/10 12:00 AM (13 years, 4 months ago)

The mexi cube would be the dirtiest plate... Just kidding haha, but very nice agar work. Its always nice to see that beautiful rhizomorphic stuff crawling across agar.

I feel pretty pro right now, I poured 25 dishes in a glove box, stored them in the fridge for 2 weeks, and have 25 sterile dishes now. After letting em sit out at room temp for a while. Yay me...

4 section dishes are so sexy and cheap, 4.70 a sleeve at cynmar.com just incase anyone needs them.


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InvisibleJavadog
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Re: My Agar Work [Re: Doctopus]
    #13212618 - 09/18/10 01:21 AM (13 years, 4 months ago)

Yes I used Cyanmar too.  I got some one and some three
section dishes.

I also got some of the polypropylene dishes that a poster
recommended. 

I agree that agar works.  I have had dishes out of the
fridge for weeks and nothing has shown any contamination.

I may just go G2G with the grain jars that I have started
with wedges.
I have had some issues with LC...but I still love it.

JD


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

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