|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
anonjon
Partially Right
Registered: 11/03/08
Posts: 6,322
|
shaking grain and senescence?
#13120186 - 08/29/10 05:49 PM (13 years, 7 months ago) |
|
|
Could how you shake affect senescence?
For example if you shake when it's only 10% colonized versus say 25%. There should be a difference in the total number of cell generations that occur? Think this number would be significant or no with regard to genetic degradation?
-------------------- The above post is fictional, hypothetical, or downright nonsensical.
|
seylm
still breathing
Registered: 04/22/10
Posts: 463
Loc: Canada
Last seen: 8 years, 1 month
|
Re: shaking grain and senescence? [Re: anonjon]
#13120263 - 08/29/10 06:02 PM (13 years, 7 months ago) |
|
|
Well shaking isn't destroying cells, it's just breaking them apart. So I would think that there would be no difference. I don't really know anything about senescence or cell generations though. I shake once at 30%.
-------------------- Easiest and Stealthiest Way to Grow Psilocybin "you may be wearing an Armani suit, but it's just pyjamas" - Mooji
|
anonjon
Partially Right
Registered: 11/03/08
Posts: 6,322
|
Re: shaking grain and senescence? [Re: seylm]
#13120306 - 08/29/10 06:09 PM (13 years, 7 months ago) |
|
|
Quote:
seylm said: Well shaking isn't destroying cells, it's just breaking them apart. So I would think that there would be no difference. I don't really know anything about senescence or cell generations though. I shake once at 30%.
I don't really know anything about <topic>. But hey, lets talk about it.
-------------------- The above post is fictional, hypothetical, or downright nonsensical.
|
Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
|
Re: shaking grain and senescence? [Re: anonjon]
#13120320 - 08/29/10 06:12 PM (13 years, 7 months ago) |
|
|
It's not the total number of divisions that is in question. It's the number of division in the history of each cell.
-------------------- You make it all possible. Doesn't it feel good?
|
anonjon
Partially Right
Registered: 11/03/08
Posts: 6,322
|
Re: shaking grain and senescence? [Re: Doc_T]
#13120368 - 08/29/10 06:22 PM (13 years, 7 months ago) |
|
|
right, thats what i mean by generations
there's got to be some optimal point where you get full colonization in the fewest possible generations, which is determined by when you shake it.
someone mathematically inclined could probably figure out if it could be significant in terms of rate of senescense
-------------------- The above post is fictional, hypothetical, or downright nonsensical.
|
Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
|
Re: shaking grain and senescence? [Re: anonjon]
#13120427 - 08/29/10 06:36 PM (13 years, 7 months ago) |
|
|
No, you end up with the same number of generations.
-------------------- You make it all possible. Doesn't it feel good?
|
anonjon
Partially Right
Registered: 11/03/08
Posts: 6,322
|
Re: shaking grain and senescence? [Re: Doc_T]
#13120511 - 08/29/10 06:50 PM (13 years, 7 months ago) |
|
|
lets say i just inoculated with a single cell, theoretically.
It will undergo a lot more generations than if I had inoculated with 100.
-------------------- The above post is fictional, hypothetical, or downright nonsensical.
|
seylm
still breathing
Registered: 04/22/10
Posts: 463
Loc: Canada
Last seen: 8 years, 1 month
|
Re: shaking grain and senescence? [Re: anonjon]
#13120521 - 08/29/10 06:52 PM (13 years, 7 months ago) |
|
|
That has nothing to do with shaking though.
-------------------- Easiest and Stealthiest Way to Grow Psilocybin "you may be wearing an Armani suit, but it's just pyjamas" - Mooji
|
k00laid
NEMO
Registered: 05/03/10
Posts: 19,636
Last seen: 8 months, 21 days
|
Re: shaking grain and senescence? [Re: seylm]
#13120537 - 08/29/10 06:56 PM (13 years, 7 months ago) |
|
|
its like innoculating a jar with one grain or a tablespoon of grains.
-------------------- AMU - AMU Q & A - MyVideo Teks!
|
anonjon
Partially Right
Registered: 11/03/08
Posts: 6,322
|
Re: shaking grain and senescence? [Re: seylm]
#13120545 - 08/29/10 06:58 PM (13 years, 7 months ago) |
|
|
sure it does, i'm creating new inoculation points, or rather im instantly multiplying the number of inoculation points.
lets say u did g2g2g.... u did it with jars that had been shaken, and jars that hadn't. I'm wondering if the non-shaken dna would crap out sooner.
-------------------- The above post is fictional, hypothetical, or downright nonsensical.
|
seylm
still breathing
Registered: 04/22/10
Posts: 463
Loc: Canada
Last seen: 8 years, 1 month
|
Re: shaking grain and senescence? [Re: k00laid]
#13120563 - 08/29/10 07:01 PM (13 years, 7 months ago) |
|
|
Yeah but when you shake you're not adding more cells, you're just spreading them around.
-------------------- Easiest and Stealthiest Way to Grow Psilocybin "you may be wearing an Armani suit, but it's just pyjamas" - Mooji
|
k00laid
NEMO
Registered: 05/03/10
Posts: 19,636
Last seen: 8 months, 21 days
|
Re: shaking grain and senescence? [Re: seylm]
#13120576 - 08/29/10 07:05 PM (13 years, 7 months ago) |
|
|
what if you added 1 grain or a handful of grains from one jar to another. you are adding more cells then.
-------------------- AMU - AMU Q & A - MyVideo Teks!
|
seylm
still breathing
Registered: 04/22/10
Posts: 463
Loc: Canada
Last seen: 8 years, 1 month
|
Re: shaking grain and senescence? [Re: k00laid]
#13120591 - 08/29/10 07:08 PM (13 years, 7 months ago) |
|
|
Okay, so the more grains you add, the fewer cell generations needed to colonize that new jar. Right? Forgive me if I'm
-------------------- Easiest and Stealthiest Way to Grow Psilocybin "you may be wearing an Armani suit, but it's just pyjamas" - Mooji
|
Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
|
Re: shaking grain and senescence? [Re: anonjon]
#13120594 - 08/29/10 07:09 PM (13 years, 7 months ago) |
|
|
Quote:
anonjon said: sure it does, i'm creating new inoculation points, or rather im instantly multiplying the number of inoculation points.
But they are all the same age. And their descendants are as well. As the cells continue to divide, they start with chromosomes of the same age.
What matters is the number of cell divisions in the line of heredity between your cell and the spores mating.
-------------------- You make it all possible. Doesn't it feel good?
|
k00laid
NEMO
Registered: 05/03/10
Posts: 19,636
Last seen: 8 months, 21 days
|
Re: shaking grain and senescence? [Re: seylm]
#13120607 - 08/29/10 07:11 PM (13 years, 7 months ago) |
|
|
sounds right
-------------------- AMU - AMU Q & A - MyVideo Teks!
|
RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 1 month
|
Re: shaking grain and senescence? [Re: k00laid]
#13120703 - 08/29/10 07:33 PM (13 years, 7 months ago) |
|
|
The word 'generation' is being misused here. A generation is going from spore to mycelium, to fruit, and then back to spore, which is the starting point for the next generation.
If you shake at ten percent vs thirty percent, the total number of cell divisions at full colonization remains the same. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
|
seylm
still breathing
Registered: 04/22/10
Posts: 463
Loc: Canada
Last seen: 8 years, 1 month
|
Re: shaking grain and senescence? [Re: k00laid]
#13120704 - 08/29/10 07:33 PM (13 years, 7 months ago) |
|
|
I still don't see how it has anything to do with shaking though...
-------------------- Easiest and Stealthiest Way to Grow Psilocybin "you may be wearing an Armani suit, but it's just pyjamas" - Mooji
|
anonjon
Partially Right
Registered: 11/03/08
Posts: 6,322
|
Re: shaking grain and senescence? [Re: seylm]
#13121054 - 08/29/10 08:44 PM (13 years, 7 months ago) |
|
|
i dont think im misusing generations because i'm speaking of generations of cell divisions.
yes the total number of divisions is the same, but the generations of copies that get made along the way is different.
lets say I have a copy machine. I make 10 copies of an original, i make 10 copies of each of those, and 10 copies of each of those. I now have 1111 copies that are at most 2 generations away from the original.
But if I'd made 2 copies, and then 2 of those, and then 2....until i have 1111 copies. Now some are many generations from the original and starting to get blurry.
My secretary grabs a random copy from a pile and uses it to make more copies (a g2g inoculation). She grabs one that is 8 generations from the original. It comes out looking like crap.
That was my original thinking neway. The faster the jar colonizes, the fewer actual generations of cell division occur.
-------------------- The above post is fictional, hypothetical, or downright nonsensical.
|
seylm
still breathing
Registered: 04/22/10
Posts: 463
Loc: Canada
Last seen: 8 years, 1 month
|
Re: shaking grain and senescence? [Re: anonjon]
#13121114 - 08/29/10 08:53 PM (13 years, 7 months ago) |
|
|
Speed doesn't have anything to do with it. A certain number of divisions are required to fully colonize a jar. The number required is the same whether the divisions happen in 3 days or a week.
-------------------- Easiest and Stealthiest Way to Grow Psilocybin "you may be wearing an Armani suit, but it's just pyjamas" - Mooji
Edited by seylm (08/29/10 08:54 PM)
|
k00laid
NEMO
Registered: 05/03/10
Posts: 19,636
Last seen: 8 months, 21 days
|
Re: shaking grain and senescence? [Re: seylm]
#13121147 - 08/29/10 08:58 PM (13 years, 7 months ago) |
|
|
would the number of divisions needed not decline if you had more starting points?
-------------------- AMU - AMU Q & A - MyVideo Teks!
|
|