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Offlinehenk600
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sterilizing or pasteurize coco coir as casing?
    #13100367 - 08/25/10 01:34 PM (13 years, 7 months ago)

what would be the safest?

i searched the forum but could not find a good answer on it.
what would be the best against contaminations?

or when i use rye as spawn to coco coir as substrate,
sterilizing or pasteurize it?

greets
henk600


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Offlinek00laid
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Re: sterilizing or pasteurize coco coir as casing? [Re: henk600]
    #13100380 - 08/25/10 01:37 PM (13 years, 7 months ago)

pasteurizing will leave beneficial bacteria dead but still a viable food source for the myc.

sterilizing leaves nothing.

you will get both answers out of this thread.

i personally pasteurize.


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Offlinehenk600
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Re: sterilizing or pasteurize coco coir as casing? [Re: k00laid]
    #13100421 - 08/25/10 01:44 PM (13 years, 7 months ago)

thnx for the answer.

the reason i asked this is because my last (all)7 containers with an mix of horsepoo and coco coir mixed at 50/50 as substrate with an casing of coco were all with green mold.....
i sterrilized both.
in the begin it was ok,but after 2 weeks i had this problem.

could this 100% contamination rate because i sterilized it or could it be the horsepoo mixed in it?
should i had that 100% rate also when i pasteurized it?

i also have 1 container with rye as substrate and an casing of peat/gypsum/calcium carbonate and that one goes very well without contaminations.
it is placed in the same fruiting chamber at the same time.


i really wonder cause i dont want to make the same mistake again,
all the time waiting for only an 100% contimanation rating..


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Edited by henk600 (08/25/10 01:52 PM)

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Offlinekevincoughlin
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Re: sterilizing or pasteurize coco coir as casing? [Re: henk600]
    #13100425 - 08/25/10 01:45 PM (13 years, 7 months ago)

Theirs so many threads on this lol. From what I remember, putting the coir in a bucket with boiling water and closing the lid is the most economic, and easiest way, therefor the most popular.

Also this method was said by some as an actual sterilization after a ton of arguments. The coir is brought up the appropriate sterilization temps for over an hour.


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Their are no stupid questions, just stupid people - Mrs Garrison

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Offlinek00laid
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Re: sterilizing or pasteurize coco coir as casing? [Re: kevincoughlin]
    #13100445 - 08/25/10 01:50 PM (13 years, 7 months ago)

Quote:

henk600 said:
could this 100% contamination rate because i sterilized it or could it be the horsepoo mixed in it?





sterile horse poo will almost always contam before the myc colonizes it all. thats why we pasteurize. we don't care what is in the bulk sub. only that our myc gets to it first.

Quote:

kevincoughlin said:
Theirs so many threads on this lol. From what I remember, putting the coir in a bucket with boiling water and closing the lid is the most economic, and easiest way, therefor the most popular.

Also this method was said by some as an actual sterilization after a ton of arguments. The coir is brought up the appropriate sterilization temps for over an hour.




a bucket will not hold sterilization temperatures for an hour.

it will however hold 140-160 for about an hour. but i find this a very unreliable way to pasteurize. i bring everything to field capacity. load into jars. put them into a pot with water , off the bottom of course, jam a meat thermometer into one of the jars.
boil the water then turn it off.

the heat will stay 140-160 inside your jars for an hour.
turn the heat back on if needed.


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Offlinehenk600
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Re: sterilizing or pasteurize coco coir as casing? [Re: k00laid]
    #13100482 - 08/25/10 01:57 PM (13 years, 7 months ago)

thnx man now i know why all of my containers were contimanated.
it was the horsepoo which was sterilized......

next time i will pasteurize the coco coir!
(and leave the horse poo out of it.....)

damn i have a lot to learn,i readed so much but how could i make the folt of sterilize the coco/horse poo substrate....

thnx for the advice you really help me with this!
5 shrooms for you.


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Edited by henk600 (08/25/10 02:00 PM)

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Offlinek00laid
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Re: sterilizing or pasteurize coco coir as casing? [Re: henk600]
    #13100497 - 08/25/10 01:59 PM (13 years, 7 months ago)

Quote:

henk600 said:
damn i have a lot to learn,




me too :P
i learned most everything i know just reading threads on mush cult.

people here know what they talkin about


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OfflineThe shroomy 1
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Re: sterilizing or pasteurize coco coir as casing? [Re: henk600]
    #13100949 - 08/25/10 03:38 PM (13 years, 7 months ago)

I'm a bit confused.  Are you asking if your sterilize coir for a "Casing Layer"  or as a Substrate?  It's very important to make that distinction to get the appropriate answer.


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AMU Q&A thread.

Edited by The shroomy 1 (08/25/10 03:40 PM)

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Offlinek00laid
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Re: sterilizing or pasteurize coco coir as casing? [Re: henk600]
    #13100987 - 08/25/10 03:45 PM (13 years, 7 months ago)

Quote:

henk600 said:
i use rye as spawn to coco coir as substrate,






Quote:

henk600 said:
1 container with rye as substrate and an casing of peat/gypsum/calcium carbonate and that one goes very well without contaminations.





i think he knows the difference.


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OfflineLennybernadino
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Re: sterilizing or pasteurize coco coir as casing? [Re: k00laid]
    #13101054 - 08/25/10 03:55 PM (13 years, 7 months ago)

Roger rabbit said that Green mold will not grow on straight Coco coir. Having a bunch of green mold contaminated grin spawn I had to try it! so I picked up a bunch of conocunt husks from the markt, cut them up a bit pasteruisedthem and put the green and cubensis spawn in the coconut fibers, and it ereally worked! the mycelium colonized the conocnut fiber lreaving the green mold to eat the leftover grain spwawn, bt could not touch the coconut fiber .

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OfflineThe shroomy 1
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Re: sterilizing or pasteurize coco coir as casing? [Re: k00laid]
    #13101091 - 08/25/10 04:02 PM (13 years, 7 months ago)

Quote:

k00laid said:
Quote:

henk600 said:
i use rye as spawn to coco coir as substrate,






Quote:

henk600 said:
1 container with rye as substrate and an casing of peat/gypsum/calcium carbonate and that one goes very well without contaminations.





i think he knows the difference.





I guess I've never thought of casing straight rye.  In the first quote he refers to using coir as a substrate.

In either case, Substrate OR Casing Layer..... You pasteurize.  The only thing you sterilize is the substance you are going to inoculate and use as your spawn.  Also, you Pasteurize to destroy competitive molds while leaving the beneficial microorganisms behind.  You don't want to Destroy everything so that Mycelium "uses it for food".:rofl:


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Offlinek00laid
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Re: sterilizing or pasteurize coco coir as casing? [Re: The shroomy 1]
    #13101121 - 08/25/10 04:09 PM (13 years, 7 months ago)

the search engine provides ambiguous results as to the pasteurization or sterilization of bulk substrates. i know of a few around here to strictly sterilize their bulk substrates and get good results.

do whatever works for you, and if one isnt working try the other.


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Offlinemad capper
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Re: sterilizing or pasteurize coco coir as casing? [Re: k00laid]
    #13101162 - 08/25/10 04:18 PM (13 years, 7 months ago)

I just pastuerize my coco/verm/gyp bulk in quart jars at between 140 to 160 degrees for an hour. Ive read sterilizing brings higher temps that kill beneficial qualities of the substrate.


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