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InvisibleMrMaddHatter
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One year G2G
    #1309252 - 02/15/03 06:17 AM (14 years, 7 months ago)

AFOAF has been doing grain to grain with one cloned strain. After a year or so of cloning and cloning, it contaminates every time it is transferred to another jar. Does a clone loose its vitality after a while?
Sometimes it will get 80% colonized, then contaminate. AFOAF never had any problems before with grain.
Would starting from a fresh print/syringe (?new genetics?) solve the problem?

Probably not an advanced cultivation question, but afoaf needs advanced cultivators answers. :tongue:


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OfflineAnnoA
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Re: One year G2G [Re: MrMaddHatter]
    #1309284 - 02/15/03 06:38 AM (14 years, 7 months ago)

I guess the master jar is coontaminated itself.

It?s a good idea to start from spores from time to time, yes.


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OfflineBaby_Hitler
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Re: One year G2G [Re: MrMaddHatter]
    #1309517 - 02/15/03 09:33 AM (14 years, 7 months ago)

Reisolation by agar might could save it if it is contaminated. Does it always get the same contamination. If so, what does it look like?

How did he go about choosing this particular clone?


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Offlinecanid
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Re: One year G2G [Re: MrMaddHatter]
    #1309618 - 02/15/03 10:32 AM (14 years, 7 months ago)

isolates do tend to loose something over time, it's called senescence.


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InvisibleMrMaddHatter
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Re: One year G2G [Re: MrMaddHatter]
    #1309756 - 02/15/03 12:09 PM (14 years, 7 months ago)

The strain was started from a print scraped into a quart of grain. Once one master was made, lots more were made in an lysol sprayed area. Everything went fine using the same technique for atleast 15 generations. AFOAF kinda figured the spawn might be the problem so he soaked some in h202 for 30 min. Out of 10, 3 fully colonized. The others hit by green mold. The ones that made it grew very poorly.
Most of the green mold seems to be starting growth around the spawn kernels, so that leads AFOAF to believe its the spawn.

Thanx for the input people  :cool:


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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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