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Amazon Shop: ½ Pint Jars, pH Test Strips, Pressure Cooker, Wild Bird Seed

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Offlinesancho
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grain contams.....
    #1309154 - 02/15/03 04:41 AM (15 years, 4 months ago)

when you soak your grain in water overnight, why couldnt you make a 25% bleach, 75% water solution to let it soak in. wouldnt the bleach kill all the nasty bacterial endospores? then wash it off and stick it in a pot of boiling water for a few hours to kill the rest of the bacteria that was either in the jars of got into the grain while transplanting it into the jars......i dont have a pressure cooker and was wondering if that would work. would it? thanks


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OfflineAnnoA
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Re: grain contams..... [Re: sancho]
    #1309155 - 02/15/03 04:44 AM (15 years, 4 months ago)

Try it.
Don?t forget that by soaking the grain the soaking fluid, in your case partially bleach, enters the grain itself.


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Offlinesancho
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Re: grain contams..... [Re: Anno]
    #1309164 - 02/15/03 04:49 AM (15 years, 4 months ago)

im using a bird seed mix i picked up at truevalue hardware for about 1 dollar. its sunflower seeds with a bunch of others. will that work? also, what im planning on doing is letting it soak in the bleach solution and then letting it soak in water....ill post something if it works..........


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InvisibleMushMushi
Registered: 08/23/02
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Re: grain contams..... [Re: sancho]
    #1309166 - 02/15/03 04:50 AM (15 years, 4 months ago)

What if you use H2O2 instead?


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OfflineAnnoA
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Re: grain contams..... [Re: sancho]
    #1309176 - 02/15/03 04:59 AM (15 years, 4 months ago)

Also don?t forget that the pH of the grain will likely be very high due to the bleach.


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Invisibledeprave
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Registered: 11/12/02
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Re: grain contams..... [Re: Anno]
    #1309272 - 02/15/03 06:31 AM (15 years, 4 months ago)

sancho yer avatar cause me to smirk  :smirk: :smirk: :smirk:


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Invisibledeprave
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Re: grain contams..... [Re: deprave]
    #1309274 - 02/15/03 06:32 AM (15 years, 4 months ago)

my friend would be weary of soaking future substrate in bleach


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Offlinesancho
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Re: grain contams..... [Re: deprave]
    #1309356 - 02/15/03 07:35 AM (15 years, 4 months ago)

damn those endospores are tough buggers. after researching endospores, i discovered that the ONLY things that will kill them is either boiling them for over two hours, a pressure cooker, or boiling them for 1/2 hour for 3 days in a row. bleach or any type of dissenfectant *sp?* soap is futile. here are some pretty good sites about sterility.

http://www.bact.wisc.edu/microtextbook/ControlGrowth/sterilization.html

http://www.gpc.peachnet.edu/~cmcallis/1913/Ch7PhysicalControlofMicrobes040202revisions.htm

guess im going to just boil the jars for 3 days for 1.5 hours.....hope it will work. also, if i tried the honey tek and had mycelium growing, wouldnt it give the mycelium a better chance of survival than if i just injected the spores into the birdseed-filled jars? thanks  :grin:


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Amazon Shop: ½ Pint Jars, pH Test Strips, Pressure Cooker, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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