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OfflineGrizzlbutt
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Are myc metabolites anti-bacterial?
    #13087459 - 08/22/10 06:06 PM (13 years, 6 months ago)

I had a few wbs jars with what looked like a pinkish bacterial contamination in the condensation at the bottom of the jars early on in colonization (maybe 30% colonized). I tossed two of them but kept one in quarantine to study what happens. Growth was obviously stalled and there was very little "fluff" to it. I noticed an increase in what looked like, what I understand to be, metabolites in the jar (yellowish fluid) and then the pink stuff vanished. Any condensation in the jar is now clear and the mycelium is fluffing back up and spreading out again.
  Is what I saw correct or was it just a coincidence? Looked to me like the metabolites (if that's what I was actually looking at) wiped out the bacteria so the mycelium could continue growing. If that's the case then that would mean that metabolites aren't really "piss" at all, right? Wish I would have taken a picture of the metabolites but now it just looks like a normal 1/2 colonized jar.
  I read somewhere that the Tai strains are more resistant to contams (it's a Koh S. strain) but I've also heard a cube is a cube (except PE). Can mycelium go on the offensive like that? And if so, is there a was to increase metabolite secretion?


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Offlinecurenado
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Re: Are myc metabolites anti-bacterial? [Re: Grizzlbutt]
    #13087494 - 08/22/10 06:20 PM (13 years, 6 months ago)

There are a couple variables on that - one is that mushrooms change the antibiotic "coat" they are wearing more than once in their development.
2) Is that penicillin metabolites are the substance we use, so it seems logical and I guess we'll test that in the lab because

Now you got me stoked on it :lol:


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OfflineGrizzlbutt
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Re: Are myc metabolites anti-bacterial? [Re: curenado]
    #13087539 - 08/22/10 06:34 PM (13 years, 6 months ago)

I should mention that the night before I saw the pink contamination I had an incubator malfunction that caused my incubator to climb up to over 100 deg F for at least 2-4 hours. The thermometer needle was buried at 120+ deg directly under the lamp. I'm pretty sure this helped jump start the pink stuff.


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Offlinecurenado
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Re: Are myc metabolites anti-bacterial? [Re: Grizzlbutt]
    #13087581 - 08/22/10 06:43 PM (13 years, 6 months ago)

Oh yeah no doubt. The pink only comes on us after Spring when the heat rises and the jars are ten degrees hotter than they were. It also seems to be weak anyway - but I am still testing your theory in vitro. Have just the (jalisco) metabolites to do it with too.


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"The woods are lovely, dark and deep; but I have patches to keep, and jars to sterilize before I sleep...."


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OfflineRogerRabbitM
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Re: Are myc metabolites anti-bacterial? [Re: curenado]
    #13088575 - 08/22/10 10:40 PM (13 years, 6 months ago)

I've ran a lot of experiments on this.  Metabolites are not only antibiotic, but antifungal as well, when it comes to competing fungi.  Metabolites might not even be the best word for the liquids we see in our grain jars and spawn bags.  Enzymes seems like the better and more accurate term.  They also help to break down the substrate.
RR


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OfflineGrizzlbutt
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Re: Are myc metabolites anti-bacterial? [Re: RogerRabbit]
    #13088916 - 08/23/10 12:30 AM (13 years, 6 months ago)

Wow, these things just keep getting more amazing.


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Offlinecurenado
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Re: Are myc metabolites anti-bacterial? [Re: Grizzlbutt]
    #13095033 - 08/24/10 11:28 AM (13 years, 6 months ago)

Affirm RR - they are antibiotic, antifungal, antiviral

As to the pink mold - No, that was not it. The pink mold was in there. When it gets ready to bloom and sporulate it changes that color. When it has completed this it fades back to white, so while the metabolites have the properties they are not "omni-spectrum" and will affect some critters and not others.

Also - the risk of using such metabolites is that if there is anything but the mushroom in the jar (like say the white-pink-white mold) it too will be in your myco-cillin.

Pretty f*c*ing fabulous huh? This is identical to pennicillin manufacture, it is the metabolites, not the mold that are used. That is why when you brought up the point I was pretty sure you were exactly right.

Confirmed by
Dr. John Holliday, Director of Aloha Research
Stephen Peele, Curator, Florida Mycology Research Center


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Yours in the Natural State!
"The woods are lovely, dark and deep; but I have patches to keep, and jars to sterilize before I sleep...."


Edited by curenado (08/26/10 07:58 AM)


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OfflineCloneufc
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Re: Are myc metabolites anti-bacterial? [Re: curenado]
    #13136670 - 09/01/10 11:35 PM (13 years, 5 months ago)

How can you get penicillin to excrete metabolites that are safe for human consumption?  I can get mushroom mycelium to do it. All I do is raise the temperature and the mycelium will starts releasing metabolites like crazy. How do they do it with penicillin?


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InvisibleNMR.Xochipilli
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Re: Are myc metabolites anti-bacterial? [Re: Cloneufc]
    #13137231 - 09/02/10 05:21 AM (13 years, 5 months ago)



You don't have to do anything, they do it all on their own, just let the mold sit


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OfflineCloneufc
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Re: Are myc metabolites anti-bacterial? [Re: NMR.Xochipilli]
    #13137799 - 09/02/10 10:19 AM (13 years, 5 months ago)

The penicillin I have on dishes are blue. I've grown the green looking type like the one you have. My cultures are not excreting metabolites.


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Offlinecurenado
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Re: Are myc metabolites anti-bacterial? [Re: Cloneufc]
    #13138466 - 09/02/10 01:31 PM (13 years, 5 months ago)

Culture labs also have a number of media - I mean I have seen at least six different color plates for different culturing. Bet they produce more when fed specifically for it. Commercial P cultures are probably long cultured out for producers as well I would imagine.


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Offlineidunno
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Re: Are myc metabolites anti-bacterial? [Re: curenado]
    #13169570 - 09/09/10 09:40 AM (13 years, 5 months ago)

My question is- Could you stress a jar for metabolites/Enzymes collect them and use it as an antibacterial inoculation media,like a spore syringe- OR add it to an LC, to keep it clean?


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InvisibleNMR.Xochipilli
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Re: Are myc metabolites anti-bacterial? [Re: idunno]
    #13170549 - 09/09/10 01:36 PM (13 years, 5 months ago)

You could...but most people would find it unnecessary. That is how antibiotic use began but we've progressed past techniques like that. It's not hard to maintain sterile cultures if your technique is sound, and when a contamination is encountered you can use specific media constraints to keep baddies from growing(adjust pH, limit essential nutrients, ect...) or use refined antibiotics with a specific intent.


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