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OfflineTeach Me
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Registered: 08/21/10
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Attempting my first colonization of psyclobin cubensis, have many questions!
    #13082846 - 08/21/10 01:11 PM (13 years, 6 months ago)

Hello!

I would like to start out by thanking shroomey.org for being such a reliable and knowledgeable source, I don't know what I'd do if this site didn't exist.

I've performed research with Psyclobin Cubensis mushrooms twice, they were both glowing experiences and I was very very happy with the results. The last time I had experimented with the strain was December of 2009. I have been unable to procure more to continue research with them sadly.

Since this time has passed, me and a few of my fellow researchers have decided to take things into our own hands to cultivate our own psyclobin cubensis to continue our research at our own discretion anytime we choose. Therefore we ordered from sporeworks.com, a single grab bag special, also mentioning our use of shroomery.org and our membership. (Hopefully we are gifted another syringe for research!)

I am very excited to take this experiment on, I find it will be very rewarding, I have many questions, as I want to be sure everything is perfect.

I will be using this pf tek:



I have found it here a few times and hear its reliable, I just wanted to make sure and ask anyone if there are any changes or things to be done different from this video. I don't have any reason to believe so.

I also plan to use this pf tek here:

http://www.shroomery.org/forums/showflat.php/Number/11585613/fpart/1

Which I have a question about, is there really a need for the 12/12 cycle? I don't ask because I want to avoid it, I've just never heard about this when growing shrooms before, I know they need light so they know which direction to grow, but I've never heard anything about light cycles.

Secondly, how many jars can I inoculate using 1 syringe? I've heard 4, but I am not sure.

Thirdly, how many grams of wet mushrooms can I expect to yield per cake? I know every strain is different, and there is always variation, but an average would be nice, so I can know what to expect and prepare my research accordingly.

Fourthly, Would it be okay to leave the terrarium outside/outdoors? I am unsure of whether at this point contaminants is still an issue and whether there is a risk of contaminants if you leave the terrarium outside after birthing and dunking/rolling.

Finally, what is the average time between birthing and harvesting? I know there every mushroom and flush is different , I just want estimations so I can plan and adjust variables accordingly.

EDIT: Forgot one! I knew it! I have not been able to find a reliable answer about how many times a cake can be harvested and redunked-and rolled before the cake will stop producing mushrooms, I've heard twice, I've heard 3 times, I've heard 4. Does anyone what can be expected here?

I think I missed some things, but I'll repost if I do, I appreciate any help with his project! I am very excited about the outcome! Thanks again!


Edited by Teach Me (08/21/10 01:14 PM)


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InvisibleDoc_T
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Re: Attempting my first colonization of psyclobin cubensis, have many questions! [Re: Teach Me]
    #13083020 - 08/21/10 01:51 PM (13 years, 6 months ago)

When you shoot your jars, try to use as little as you can at each inoculation point, just a few drops. (Shake well first.)


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You make it all possible. Doesn't it feel good?


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OfflineTeach Me
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Re: Attempting my first colonization of psyclobin cubensis, have many questions! [Re: Doc_T]
    #13083055 - 08/21/10 02:00 PM (13 years, 6 months ago)

Well thats the thing, I want specifics/numbers/measurements that I can follow to a "T".

How many cc's are in a syringe ordered from sporeworks? How many do you inject into 1 jar? I haven't found a single post on there or found anything online that says how much should be injected into each hole in the jar or how many should 1 syringe make.


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OfflineJonnyBtreed
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Re: Attempting my first colonization of psyclobin cubensis, have many questions! [Re: Teach Me]
    #13083125 - 08/21/10 02:10 PM (13 years, 6 months ago)

Quote:

Teach Me said:
Well thats the thing, I want specifics/numbers/measurements that I can follow to a "T".






about 1 ml per jar, so one syringe will do an average of ten jars. Sporeworks along with most others send 10ml syringes unless they specify otherwise.


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Offlineandnothinghurt
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Registered: 08/20/09
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Re: Attempting my first colonization of psyclobin cubensis, have many questions! [Re: Teach Me]
    #13083132 - 08/21/10 02:12 PM (13 years, 6 months ago)

Hey, Teach Me! I'm also a newbie, but have found the search function to be pretty helpful! Whenever you think you haven't found answers on here, try different search terms. Keywords are the most helpful. ("jars per syringe" rather than "how many jars can I inoculate with one syringe")

A syringe typically contains 10cc's of spore solution. An average of 1cc per jar is the norm, with 1/4cc per hole! (4 equidistant holes on each jar lid)

My first syringe inoc'd 8 jars easily, and had I been a bit more careful I probably could've made it 10.


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Re: Attempting my first colonization of psyclobin cubensis, have many questions! [Re: Teach Me]
    #13083175 - 08/21/10 02:19 PM (13 years, 6 months ago)

Quote:

Teach Me said:
Well thats the thing, I want specifics/numbers/measurements that I can follow to a "T".




Three drops in each hole, times four holes. You'll use about 1/2 to 3/4 mL per jar, so you can easily do 12 to 15 and have a bit left over.

Less is more with spores.

But really, you're going to have to lighten up on teh demanding tone. What you want is really irrelevant to most of us.


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You make it all possible. Doesn't it feel good?


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OfflineRogerRabbitM
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Re: Attempting my first colonization of psyclobin cubensis, have many questions! [Re: Doc_T]
    #13083239 - 08/21/10 02:34 PM (13 years, 6 months ago)

Quote:

Which I have a question about, is there really a need for the 12/12 cycle? I don't ask because I want to avoid it, I've just never heard about this when growing shrooms before, I know they need light so they know which direction to grow, but I've never heard anything about light cycles.




Light isn't to tell them which way to grow.  Light is a pinning trigger and mycelium uses light for energy.  A 12/12 cycle is best. Use lights that are labeled 6500 Kelvin.  This is the color spectrum that gives best performance.

Quote:

psyclobin cubensis




The term you're looking for is Psilocybe cubensis.  Psilocybe is a four syllable word, pronounced  sill as in window sill, ahh as in stick out your tongue and say ahh for the doctor, ci as in cigarette, bee as in honey bee.  Accent is on the second syllable.  Sill-ahh'-ci-bee.
RR


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OfflineTeach Me
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Re: Attempting my first colonization of psyclobin cubensis, have many questions! [Re: Teach Me]
    #13083312 - 08/21/10 02:56 PM (13 years, 6 months ago)

Many thanks for all the replies so far!

Thank you for clearing up the syringe/injection question, I thought I would only get about 4 jars per syringe, I'm glad to hear its in the 10-15 range.

Also I did not mean to come off demanding in the least, I just wanted to enhance the fact that I'm the kind of of person who likes exacts when following instructions especially in say recipes or in this case growing. I just want to make sure that if I mess up on something, I can know what I can pinpoint wrong, so I can say to myself "Nope, I injected the right amount into each jar, couldn't be that". I just want to follow instructions really well, that's all. :laugh:

And yes, I made an error when typing out the name, I knew that, but it spelled checked it that way and I hurridly continued my post. Thanks for the clarification though.

This leaves me with the two questions I really have left to ask:

The safety/possibility of leaving the shotgun terrarium outside? (I live in the South, where the temperatures are pretty warm (especially in the summer) and humidity can be high, but also dry sometimes. I don't know if its already assumed that they should be indoors at all times, but I wanted to ask anyway. I believe I saw a thread there a few months back lurking about a guy who was growing them in a tub outside (not cakes though), but I can't find it, and haven't seen any other examples on here since.

The amount of times a single cake can produce flushes before stopping completely?

Also: I wanna do my HW on terminology: FAE means (Free Air Exchange?), RH means (Room Humidity?), GE is (Gas Exchange)? Is that right?

Thank you for all your help so far, sorry if I come off...negatively or pushy? I really ask with all respect and positivity! :smile:


Edited by Teach Me (08/21/10 02:57 PM)


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OfflinePandor
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Re: Attempting my first colonization of psyclobin cubensis, have many questions! [Re: Teach Me]
    #13083426 - 08/21/10 03:43 PM (13 years, 6 months ago)

Grow them inside.

Use the glossary of abbreviations.

I know it is easier to ask and I do that a lot as well. But you must learn the basics yourself and show your willing to put effort in.


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Re: Attempting my first colonization of psyclobin cubensis, have many questions! [Re: Pandor]
    #13083544 - 08/21/10 04:16 PM (13 years, 6 months ago)

Quote:

I just wanted to enhance the fact that I'm the kind of of person who likes exacts when following instructions especially in say recipes or in this case growing




It's less like baking and more like making soup. It won't always be exact.
And your idea of exact won't be the same as somebody else's- wait until you get to spawn ratios.

And I'd like to enhance emphasize the fact that you get what you get if you ask a question here.
Feel free to use the search engine as an alternative.


--------------------
You make it all possible. Doesn't it feel good?


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Offlinecptinsan0
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Re: Attempting my first colonization of psyclobin cubensis, have many questions! [Re: Doc_T]
    #13083581 - 08/21/10 04:27 PM (13 years, 6 months ago)

sillohsyebin.... :smile:


--------------------
Twenty years from now you will be more disappointed by the things that you didn't do than by the ones you did do. So throw off the bowlines. Sail away from the safe harbor. Catch the trade winds in your sails. Explore. Dream. Discover.- Mark Twain


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OfflineTeach Me
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Re: Attempting my first colonization of psyclobin cubensis, have many questions! [Re: cptinsan0]
    #13083606 - 08/21/10 04:34 PM (13 years, 6 months ago)

Emphasize, thats the word I was looking for! :P

Thanks again for everything, I think I have it all down pat, and will be using search to look up info before posting.

Yes I know I'm not entitled to any help, I just ask, and hope!


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