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OfflineGrizzdude
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Registered: 07/19/09
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How do I save a strain?
    #13038506 - 08/11/10 08:23 PM (14 years, 5 months ago)

I have this really kick ass oakridge strain that I isolated on agar a while back. It colonizes jars in 4 days and same with monotub's. I have been doing G2G with WBS jars to keep it going. I want to take a break from the growing for a bit so how do I preserve this stain? I got colonized WBS jars and an agar plate from like 4 months ago. Any tips?


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OfflineFungal-one
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Registered: 07/14/10
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Re: How do I save a strain? [Re: Grizzdude]
    #13038673 - 08/11/10 08:54 PM (14 years, 5 months ago)

You're doing all that advanced shit and you don't know how to do what you're asking?

I smell bullshit.


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Never judge a man until you walk a mile in his shoes. But, by that time you're a mile away and you got his shoes, so fuck em.

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InvisibleDoc_T
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Posts: 42,395
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Re: How do I save a strain? [Re: Fungal-one]
    #13038688 - 08/11/10 08:56 PM (14 years, 5 months ago)

agar -> fridge


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You make it all possible. Doesn't it feel good?

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OfflineFISTFUL.OF.CUBES
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Registered: 10/01/09
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Re: How do I save a strain? [Re: Fungal-one]
    #13038691 - 08/11/10 08:57 PM (14 years, 5 months ago)

Spore prints? (serious question mark from a serious noob)

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Offlinekechlesurf
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Registered: 11/30/07
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Re: How do I save a strain? [Re: Grizzdude]
    #13038693 - 08/11/10 08:58 PM (14 years, 5 months ago)

Myc can survive for a little while in the fridge, or just fruit it and print it, i agree with fungal, to be doing agar you should know what to do in your down time

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Invisibleuncle_rico
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Re: How do I save a strain? [Re: FISTFUL.OF.CUBES]
    #13038697 - 08/11/10 08:59 PM (14 years, 5 months ago)

Do a search of 'master slants'

That's what you want.


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Offlinekechlesurf
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Re: How do I save a strain? [Re: uncle_rico]
    #13038708 - 08/11/10 09:00 PM (14 years, 5 months ago)

here's a link to one, had it saved in favorites

http://www.shroomery.org/forums/showflat.php/Number/12968316

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Invisiblecreamcorn
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Registered: 03/13/06
Posts: 2,962
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Re: How do I save a strain? [Re: Grizzdude]
    #13038730 - 08/11/10 09:05 PM (14 years, 5 months ago)

i guess it depends on how long you want to keep it viable, and how much work you're willing to do.

the easiest thing would be to bag your agar dish and stick it in the fridge. it should stay good a few more months.

likewise, your grain jar itself will keep in the fridge several months (6ish)

these aren't hard limits. it may last quite a bit longer, on the order of a couple years.

a rule of thumb if you want to play it very safe, is to transfer to new agar every 6-12 months or so. each time you transfer, alternate mediums. so if you used PDA this time, revive it on some malt extract in 6 months; keep that for 6 months, revive it on some PDA again (or something different, even) and so on. the change in medium forces the mycelium to adapt its enzymes, think of it as kind of keeping it on its toes, to keep it vigorous.

many folks like to use slants for long term storage, essentially agar in test tubes, with the tubes held at an angle while the agar cools (hence, a slanted surface). not sure if there's a benefit from a time perspective, other than they take up less space and are sturdy. :smile:

it's also possible to keep mycellium in cold storage, simply suspended in nutrient-deplete distilled water, for long periods.

search the advanced forum for "storage" for some more ideas.

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Offlinekechlesurf
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Re: How do I save a strain? [Re: creamcorn]
    #13038743 - 08/11/10 09:07 PM (14 years, 5 months ago)

Have to trust the "trusted cultivators", they know what they are talking about

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OfflineDamion5050
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Re: How do I save a strain? [Re: kechlesurf]
    #13038755 - 08/11/10 09:11 PM (14 years, 5 months ago)

I do agar dishes in the fridge then every 12 months bring them out and transfer to a new dish.

I also do vacutainers of LC in the fridge for 6 months..

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InvisibleJavadog
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Registered: 05/03/10
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Re: How do I save a strain? [Re: kechlesurf]
    #13038789 - 08/11/10 09:17 PM (14 years, 5 months ago)

They do.

There is a paper on very long term storage that appears
to be the duration winner.

The idea is that you store just mycelia in distilled H2O.
Without nutrients, the fungus goes dormant.

Good Luck!

JD


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Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org

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OfflineGrizzdude
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Registered: 07/19/09
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Re: How do I save a strain? [Re: Javadog]
    #13043409 - 08/12/10 08:07 PM (14 years, 5 months ago)

Thanks for the advise guys! lots of good reply's here. For the mean time it looks like I can just keep the agar dish in the fridge for a few more months then do a transfer. I'm going to have to look into the master slants. Thanks for the replys everyone!


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OfflineSN122A
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Registered: 10/09/09
Posts: 223
Loc: Flag
Last seen: 2 years, 1 month
Re: How do I save a strain? [Re: Grizzdude]
    #13043544 - 08/12/10 08:35 PM (14 years, 5 months ago)

Yeh I do mainly petri's and slants but a few vials of LC were usable after 6 months. 

If you looking for a nice storage container I buy a nice whiskey from time to time and find I can stack the petris almost flush in the cardboard tube the bottle comes in :smile: 

Just remember I think its the 80mm dishes for a standard tube, so you need a fatter one for the 90mm dishes...

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