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Offline1dontcarry
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New grow to eliminate contams
    #13034271 - 08/10/10 10:23 PM (14 years, 5 months ago)

should i clean newly bought jars out with something before i fill them with substrate? or are they good


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Invisiblem00nshine
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Re: New grow to eliminate contams [Re: 1dontcarry]
    #13034297 - 08/10/10 10:29 PM (14 years, 5 months ago)

They're good.


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InvisibleDoc_T
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Re: New grow to eliminate contams [Re: 1dontcarry]
    #13034299 - 08/10/10 10:29 PM (14 years, 5 months ago)

No need. New jars are clean.


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Offline1dontcarry
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Re: New grow to eliminate contams [Re: Doc_T]
    #13034308 - 08/10/10 10:32 PM (14 years, 5 months ago)

Now another question:

between each jar in inoculation, can you sterilize with alcohol rather than flame to prevent cross contamination?


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Offlinenexus1946
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Re: New grow to eliminate contams [Re: 1dontcarry]
    #13034309 - 08/10/10 10:32 PM (14 years, 5 months ago)

I always clean them anyway...but that's me. Better safe than sorry.


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Re: New grow to eliminate contams [Re: 1dontcarry]
    #13034322 - 08/10/10 10:36 PM (14 years, 5 months ago)

Just flame sterilize before you shoot up those jars. You can use alcohol, but that only really gets the outside of the needle. I do both sometimes neurotically. :shrug:


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Offline1dontcarry
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Re: New grow to eliminate contams [Re: 1dontcarry]
    #13034346 - 08/10/10 10:41 PM (14 years, 5 months ago)

is flame sterilization by bic lighter alright? i want to avoid buying an alcohol lamp tho i know i will in time. but right now my choices are alcohol and bic lighter for sterilization of the needle. so what to use?

secondly, if i were to clean the jars before using them. what would be best?


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OfflineRogerRabbitM
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Re: New grow to eliminate contams [Re: 1dontcarry]
    #13034349 - 08/10/10 10:41 PM (14 years, 5 months ago)

Quote:

1dontcarry said:
Now another question:

between each jar in inoculation, can you sterilize with alcohol rather than flame to prevent cross contamination?




Alcohol doesn't sterilize.  Use a flame as directed.  Sterile procedures weren't developed to make growing mushrooms harder.  They were developed because they work.
RR


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Offline1dontcarry
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Re: New grow to eliminate contams [Re: 1dontcarry]
    #13034368 - 08/10/10 10:45 PM (14 years, 5 months ago)

will a bic ligher work tho? as in a disposable one?


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Invisiblem00nshine
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Re: New grow to eliminate contams [Re: 1dontcarry]
    #13034374 - 08/10/10 10:46 PM (14 years, 5 months ago)

Works for me.


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Offlineuber noob
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Re: New grow to eliminate contams [Re: RogerRabbit]
    #13034391 - 08/10/10 10:50 PM (14 years, 5 months ago)

I'm still going through my first grow (cakes now in the FC) and I used a bic lighter for inoculation and worked out fine with 1 out of 12 jars contaminated. I used a Lysol wipe after every injection and flame sterilization to clean off the dirt/char.

I'd use an alcohol lamp if I had one readily available because everyone seems to recommend them.

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Offline1dontcarry
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Re: New grow to eliminate contams [Re: m00nshine]
    #13034401 - 08/10/10 10:52 PM (14 years, 5 months ago)

uber noob, did you use a glove box in inoculation?


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OfflinePfffffff
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Re: New grow to eliminate contams [Re: 1dontcarry]
    #13034536 - 08/10/10 11:37 PM (14 years, 5 months ago)

It doesn't have to be fancy man...grab a candle...or a bic...anything with a flame is hot as hell and works just hold it there til u see it glow, then cool for a bit in an alcohol soaked paper towel or wipe.  Give the alcohol a sec to evaporate off and go to town.


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Re: New grow to eliminate contams [Re: Pfffffff]
    #13035284 - 08/11/10 05:42 AM (14 years, 5 months ago)

DO NOT wipe your sterile needle with ANYTHING!

FFS guys, think this through- you go to get your flu shot, the nurse wipes your arm with alc,
then opens a sterile needle and
without touching anything
performs the injection.


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Offline1dontcarry
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Re: New grow to eliminate contams [Re: Doc_T]
    #13035371 - 08/11/10 07:15 AM (14 years, 5 months ago)

Quote:

Doc_T said:
DO NOT wipe your sterile needle with ANYTHING!

FFS guys, think this through- you go to get your flu shot, the nurse wipes your arm with alc,
then opens a sterile needle and
without touching anything
performs the injection.





why do you say this? everyone else is saying other shit. i just want to know your reasoning


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InvisibleDoc_T
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Re: New grow to eliminate contams [Re: 1dontcarry]
    #13035384 - 08/11/10 07:19 AM (14 years, 5 months ago)

Sterile is sterile. If you wipe it, it ISN'T STERILE!

No way anything is cleaner than that red-hot needle. No way.

Again, consider your experience in life. Have you ever seen a nurse or other professional touch a needle with anything?
Ask next time- "aren't you going to wipe the needle?", see what (s)he says.


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Invisibleanonjon
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Re: New grow to eliminate contams [Re: Doc_T]
    #13035400 - 08/11/10 07:34 AM (14 years, 5 months ago)

besides what doc said, the needle cools instantly when you depress the plunger anyway.


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Re: New grow to eliminate contams [Re: anonjon]
    #13035405 - 08/11/10 07:37 AM (14 years, 5 months ago)

That too. No need to cool it, you aren't 'wasting spores'.


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Offline1dontcarry
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Re: New grow to eliminate contams [Re: Doc_T]
    #13035972 - 08/11/10 11:26 AM (14 years, 5 months ago)

Thanks guys, you've been more than helpful. Another question! when steam sterilizing, is a vegetable steamer like This:



okay without a rack? like can the second pot be inserted as a "rack"?
Just want some opinions on it :laugh: thanks


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Offline2ndChancesRDivine
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Re: New grow to eliminate contams [Re: 1dontcarry]
    #13036055 - 08/11/10 11:46 AM (14 years, 5 months ago)

Why not just put some jar lids on the bottom?


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Offline1dontcarry
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Re: New grow to eliminate contams [Re: 1dontcarry]
    #13036106 - 08/11/10 12:00 PM (14 years, 5 months ago)

see, the thing is, im doing the jar lids on another pot. Of course i have enough lids but why use them if you don't have to


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Invisible13shroomsM
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Re: New grow to eliminate contams [Re: Doc_T]
    #13036129 - 08/11/10 12:06 PM (14 years, 5 months ago)

Quote:

Doc_T said:
Sterile is sterile. If you wipe it, it ISN'T STERILE!

No way anything is cleaner than that red-hot needle. No way.

Again, consider your experience in life. Have you ever seen a nurse or other professional touch a needle with anything?
Ask next time- "aren't you going to wipe the needle?", see what (s)he says.





:yesnod::thumbup:


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Offline1dontcarry
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Re: New grow to eliminate contams [Re: 13shrooms]
    #13036350 - 08/11/10 12:51 PM (14 years, 5 months ago)

thanks for tips on needle sterilastion doc, and thanks for confirm shrooms.

Does anyone have an answer for my question about the pot on page one? is this okay to use without a rack?


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OfflineDrewz
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Re: New grow to eliminate contams [Re: 1dontcarry]
    #13036393 - 08/11/10 01:00 PM (14 years, 5 months ago)

Don't let them touch the sides or water and you'll be fine. But use the lids, sounds so much better to just follow the teks..

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Re: New grow to eliminate contams [Re: Drewz]
    #13036498 - 08/11/10 01:25 PM (14 years, 5 months ago)

just dont let them sit on the bottom or you will burn the contents of your jars, steam is the key so lifting them up out of the water is wise.

I use a ceramic plate on the bottom of my pot. :sun:


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