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Offline1dontcarry
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New grow to eliminate contams
    #13034271 - 08/10/10 10:23 PM (14 years, 5 months ago)

should i clean newly bought jars out with something before i fill them with substrate? or are they good


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Invisiblem00nshine
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Re: New grow to eliminate contams [Re: 1dontcarry]
    #13034297 - 08/10/10 10:29 PM (14 years, 5 months ago)

They're good.


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InvisibleDoc_T
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Re: New grow to eliminate contams [Re: 1dontcarry]
    #13034299 - 08/10/10 10:29 PM (14 years, 5 months ago)

No need. New jars are clean.


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Offline1dontcarry
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Re: New grow to eliminate contams [Re: Doc_T]
    #13034308 - 08/10/10 10:32 PM (14 years, 5 months ago)

Now another question:

between each jar in inoculation, can you sterilize with alcohol rather than flame to prevent cross contamination?


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Offlinenexus1946
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Re: New grow to eliminate contams [Re: 1dontcarry]
    #13034309 - 08/10/10 10:32 PM (14 years, 5 months ago)

I always clean them anyway...but that's me. Better safe than sorry.


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Re: New grow to eliminate contams [Re: 1dontcarry]
    #13034322 - 08/10/10 10:36 PM (14 years, 5 months ago)

Just flame sterilize before you shoot up those jars. You can use alcohol, but that only really gets the outside of the needle. I do both sometimes neurotically. :shrug:


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Offline1dontcarry
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Re: New grow to eliminate contams [Re: 1dontcarry]
    #13034346 - 08/10/10 10:41 PM (14 years, 5 months ago)

is flame sterilization by bic lighter alright? i want to avoid buying an alcohol lamp tho i know i will in time. but right now my choices are alcohol and bic lighter for sterilization of the needle. so what to use?

secondly, if i were to clean the jars before using them. what would be best?


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OfflineRogerRabbitM
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Re: New grow to eliminate contams [Re: 1dontcarry]
    #13034349 - 08/10/10 10:41 PM (14 years, 5 months ago)

Quote:

1dontcarry said:
Now another question:

between each jar in inoculation, can you sterilize with alcohol rather than flame to prevent cross contamination?




Alcohol doesn't sterilize.  Use a flame as directed.  Sterile procedures weren't developed to make growing mushrooms harder.  They were developed because they work.
RR


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Offline1dontcarry
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Re: New grow to eliminate contams [Re: 1dontcarry]
    #13034368 - 08/10/10 10:45 PM (14 years, 5 months ago)

will a bic ligher work tho? as in a disposable one?


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Invisiblem00nshine
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Re: New grow to eliminate contams [Re: 1dontcarry]
    #13034374 - 08/10/10 10:46 PM (14 years, 5 months ago)

Works for me.


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Offlineuber noob
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Re: New grow to eliminate contams [Re: RogerRabbit]
    #13034391 - 08/10/10 10:50 PM (14 years, 5 months ago)

I'm still going through my first grow (cakes now in the FC) and I used a bic lighter for inoculation and worked out fine with 1 out of 12 jars contaminated. I used a Lysol wipe after every injection and flame sterilization to clean off the dirt/char.

I'd use an alcohol lamp if I had one readily available because everyone seems to recommend them.

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Offline1dontcarry
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Re: New grow to eliminate contams [Re: m00nshine]
    #13034401 - 08/10/10 10:52 PM (14 years, 5 months ago)

uber noob, did you use a glove box in inoculation?


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OfflinePfffffff
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Re: New grow to eliminate contams [Re: 1dontcarry]
    #13034536 - 08/10/10 11:37 PM (14 years, 5 months ago)

It doesn't have to be fancy man...grab a candle...or a bic...anything with a flame is hot as hell and works just hold it there til u see it glow, then cool for a bit in an alcohol soaked paper towel or wipe.  Give the alcohol a sec to evaporate off and go to town.


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InvisibleDoc_T
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Re: New grow to eliminate contams [Re: Pfffffff]
    #13035284 - 08/11/10 05:42 AM (14 years, 5 months ago)

DO NOT wipe your sterile needle with ANYTHING!

FFS guys, think this through- you go to get your flu shot, the nurse wipes your arm with alc,
then opens a sterile needle and
without touching anything
performs the injection.


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Offline1dontcarry
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Re: New grow to eliminate contams [Re: Doc_T]
    #13035371 - 08/11/10 07:15 AM (14 years, 5 months ago)

Quote:

Doc_T said:
DO NOT wipe your sterile needle with ANYTHING!

FFS guys, think this through- you go to get your flu shot, the nurse wipes your arm with alc,
then opens a sterile needle and
without touching anything
performs the injection.





why do you say this? everyone else is saying other shit. i just want to know your reasoning


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InvisibleDoc_T
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Re: New grow to eliminate contams [Re: 1dontcarry]
    #13035384 - 08/11/10 07:19 AM (14 years, 5 months ago)

Sterile is sterile. If you wipe it, it ISN'T STERILE!

No way anything is cleaner than that red-hot needle. No way.

Again, consider your experience in life. Have you ever seen a nurse or other professional touch a needle with anything?
Ask next time- "aren't you going to wipe the needle?", see what (s)he says.


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Invisibleanonjon
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Re: New grow to eliminate contams [Re: Doc_T]
    #13035400 - 08/11/10 07:34 AM (14 years, 5 months ago)

besides what doc said, the needle cools instantly when you depress the plunger anyway.


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InvisibleDoc_T
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Re: New grow to eliminate contams [Re: anonjon]
    #13035405 - 08/11/10 07:37 AM (14 years, 5 months ago)

That too. No need to cool it, you aren't 'wasting spores'.


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Offline1dontcarry
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Re: New grow to eliminate contams [Re: Doc_T]
    #13035972 - 08/11/10 11:26 AM (14 years, 5 months ago)

Thanks guys, you've been more than helpful. Another question! when steam sterilizing, is a vegetable steamer like This:



okay without a rack? like can the second pot be inserted as a "rack"?
Just want some opinions on it :laugh: thanks


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Offline2ndChancesRDivine
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Re: New grow to eliminate contams [Re: 1dontcarry]
    #13036055 - 08/11/10 11:46 AM (14 years, 5 months ago)

Why not just put some jar lids on the bottom?


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