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1dontcarry
apothecary



Registered: 06/14/10
Posts: 221
Loc: Somewhere, Safesville
Last seen: 1 year, 7 months
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New grow to eliminate contams
#13034271 - 08/10/10 10:23 PM (14 years, 5 months ago) |
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should i clean newly bought jars out with something before i fill them with substrate? or are they good
-------------------- Life's so short to be unhappy!
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m00nshine
ÜBER SHAMAN




Registered: 11/18/05
Posts: 9,774
Loc: BALLS DEEP
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Re: New grow to eliminate contams [Re: 1dontcarry]
#13034297 - 08/10/10 10:29 PM (14 years, 5 months ago) |
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They're good.
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: New grow to eliminate contams [Re: 1dontcarry]
#13034299 - 08/10/10 10:29 PM (14 years, 5 months ago) |
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No need. New jars are clean.
-------------------- You make it all possible. Doesn't it feel good?
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1dontcarry
apothecary



Registered: 06/14/10
Posts: 221
Loc: Somewhere, Safesville
Last seen: 1 year, 7 months
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Re: New grow to eliminate contams [Re: Doc_T]
#13034308 - 08/10/10 10:32 PM (14 years, 5 months ago) |
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Now another question:
between each jar in inoculation, can you sterilize with alcohol rather than flame to prevent cross contamination?
-------------------- Life's so short to be unhappy!
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nexus1946
Rebel



Registered: 06/25/09
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Re: New grow to eliminate contams [Re: 1dontcarry]
#13034309 - 08/10/10 10:32 PM (14 years, 5 months ago) |
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I always clean them anyway...but that's me. Better safe than sorry.
-------------------- Gypsum/Drywall Tek
The Story Of Russell The Texas (Cube) Bear

The human race's prospects of survival were considerably better when we were defenceless against tigers than they are today when we have become defenceless against ourselves.
-Arnold J. Toynbee
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m00nshine
ÜBER SHAMAN




Registered: 11/18/05
Posts: 9,774
Loc: BALLS DEEP
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Re: New grow to eliminate contams [Re: 1dontcarry]
#13034322 - 08/10/10 10:36 PM (14 years, 5 months ago) |
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Just flame sterilize before you shoot up those jars. You can use alcohol, but that only really gets the outside of the needle. I do both sometimes neurotically.
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1dontcarry
apothecary



Registered: 06/14/10
Posts: 221
Loc: Somewhere, Safesville
Last seen: 1 year, 7 months
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Re: New grow to eliminate contams [Re: 1dontcarry]
#13034346 - 08/10/10 10:41 PM (14 years, 5 months ago) |
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is flame sterilization by bic lighter alright? i want to avoid buying an alcohol lamp tho i know i will in time. but right now my choices are alcohol and bic lighter for sterilization of the needle. so what to use?
secondly, if i were to clean the jars before using them. what would be best?
-------------------- Life's so short to be unhappy!
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RogerRabbit
Bans for Pleasure


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Re: New grow to eliminate contams [Re: 1dontcarry]
#13034349 - 08/10/10 10:41 PM (14 years, 5 months ago) |
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Quote:
1dontcarry said: Now another question:
between each jar in inoculation, can you sterilize with alcohol rather than flame to prevent cross contamination?
Alcohol doesn't sterilize. Use a flame as directed. Sterile procedures weren't developed to make growing mushrooms harder. They were developed because they work. RR
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"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
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1dontcarry
apothecary



Registered: 06/14/10
Posts: 221
Loc: Somewhere, Safesville
Last seen: 1 year, 7 months
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Re: New grow to eliminate contams [Re: 1dontcarry]
#13034368 - 08/10/10 10:45 PM (14 years, 5 months ago) |
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will a bic ligher work tho? as in a disposable one?
-------------------- Life's so short to be unhappy!
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m00nshine
ÜBER SHAMAN




Registered: 11/18/05
Posts: 9,774
Loc: BALLS DEEP
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Re: New grow to eliminate contams [Re: 1dontcarry]
#13034374 - 08/10/10 10:46 PM (14 years, 5 months ago) |
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Works for me.
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uber noob
Stranger
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Re: New grow to eliminate contams [Re: RogerRabbit]
#13034391 - 08/10/10 10:50 PM (14 years, 5 months ago) |
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I'm still going through my first grow (cakes now in the FC) and I used a bic lighter for inoculation and worked out fine with 1 out of 12 jars contaminated. I used a Lysol wipe after every injection and flame sterilization to clean off the dirt/char.
I'd use an alcohol lamp if I had one readily available because everyone seems to recommend them.
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1dontcarry
apothecary



Registered: 06/14/10
Posts: 221
Loc: Somewhere, Safesville
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Re: New grow to eliminate contams [Re: m00nshine]
#13034401 - 08/10/10 10:52 PM (14 years, 5 months ago) |
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uber noob, did you use a glove box in inoculation?
-------------------- Life's so short to be unhappy!
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Pfffffff
I am sofa king we todd ed
Registered: 10/02/09
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Re: New grow to eliminate contams [Re: 1dontcarry]
#13034536 - 08/10/10 11:37 PM (14 years, 5 months ago) |
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It doesn't have to be fancy man...grab a candle...or a bic...anything with a flame is hot as hell and works just hold it there til u see it glow, then cool for a bit in an alcohol soaked paper towel or wipe. Give the alcohol a sec to evaporate off and go to town.
-------------------- ****************************************************
I am part of this community as a role playing
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****************************************************
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: New grow to eliminate contams [Re: Pfffffff]
#13035284 - 08/11/10 05:42 AM (14 years, 5 months ago) |
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DO NOT wipe your sterile needle with ANYTHING!
FFS guys, think this through- you go to get your flu shot, the nurse wipes your arm with alc, then opens a sterile needle and without touching anything performs the injection.
-------------------- You make it all possible. Doesn't it feel good?
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1dontcarry
apothecary



Registered: 06/14/10
Posts: 221
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Last seen: 1 year, 7 months
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Re: New grow to eliminate contams [Re: Doc_T]
#13035371 - 08/11/10 07:15 AM (14 years, 5 months ago) |
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Quote:
Doc_T said: DO NOT wipe your sterile needle with ANYTHING!
FFS guys, think this through- you go to get your flu shot, the nurse wipes your arm with alc, then opens a sterile needle and without touching anything performs the injection.
why do you say this? everyone else is saying other shit. i just want to know your reasoning
-------------------- Life's so short to be unhappy!
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: New grow to eliminate contams [Re: 1dontcarry]
#13035384 - 08/11/10 07:19 AM (14 years, 5 months ago) |
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Sterile is sterile. If you wipe it, it ISN'T STERILE!
No way anything is cleaner than that red-hot needle. No way.
Again, consider your experience in life. Have you ever seen a nurse or other professional touch a needle with anything? Ask next time- "aren't you going to wipe the needle?", see what (s)he says.
-------------------- You make it all possible. Doesn't it feel good?
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anonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
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Re: New grow to eliminate contams [Re: Doc_T]
#13035400 - 08/11/10 07:34 AM (14 years, 5 months ago) |
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besides what doc said, the needle cools instantly when you depress the plunger anyway.
-------------------- The above post is fictional, hypothetical, or downright nonsensical.
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: New grow to eliminate contams [Re: anonjon]
#13035405 - 08/11/10 07:37 AM (14 years, 5 months ago) |
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That too. No need to cool it, you aren't 'wasting spores'.
-------------------- You make it all possible. Doesn't it feel good?
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1dontcarry
apothecary



Registered: 06/14/10
Posts: 221
Loc: Somewhere, Safesville
Last seen: 1 year, 7 months
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Re: New grow to eliminate contams [Re: Doc_T]
#13035972 - 08/11/10 11:26 AM (14 years, 5 months ago) |
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Thanks guys, you've been more than helpful. Another question! when steam sterilizing, is a vegetable steamer like This:

okay without a rack? like can the second pot be inserted as a "rack"? Just want some opinions on it thanks
-------------------- Life's so short to be unhappy!
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2ndChancesRDivine
I slayed the Jabberwock!



Registered: 04/19/10
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Re: New grow to eliminate contams [Re: 1dontcarry]
#13036055 - 08/11/10 11:46 AM (14 years, 5 months ago) |
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Why not just put some jar lids on the bottom?
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All your RC are belong to me.
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