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Doobie Scooby
Stranger

Registered: 08/09/10
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Last seen: 14 years, 4 months
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Help with sterilization (Cubensis)
#13029706 - 08/09/10 11:35 PM (14 years, 5 months ago) |
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Whats up shroomery, i recently had started my own project with some amazonian cubensis, i had pulled out my pressure cooker and set it in my kitchen to cool down, after sitting for a few hours my son ended up opening the cooker to where the lid was half off, he did not touch them he just looked inside and left the lid off for about 15 min, the inoculation holes are small and there was foil some what tight over the lids, so i went ahead and inoculated in hopes that there were no contams from it being open. its day 3 and no sign of contam or mycelieum in one jar it almost looks foggy in some spots. Do you think any of my jars will colinize. Thanks
P.S i used a vermiculite brown rice substrate
Edited by Doobie Scooby (08/10/10 12:42 AM)
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Mycjunky
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Registered: 07/25/09
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Re: Help with sterilization (Cubensis) [Re: Doobie Scooby]
#13029735 - 08/09/10 11:43 PM (14 years, 5 months ago) |
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I seriously doubt he did any harm you should be fine. Good luck and welcome to the shroomery.
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JackClements
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Registered: 09/11/09
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Last seen: 14 years, 3 months
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Re: Help with sterilization (Cubensis) [Re: Doobie Scooby]
#13029738 - 08/09/10 11:44 PM (14 years, 5 months ago) |
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yes
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jokefox
Top of the chain



Registered: 12/22/09
Posts: 6,231
Loc: never where I should be
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Re: Help with sterilization (Cubensis) [Re: JackClements]
#13029789 - 08/09/10 11:56 PM (14 years, 5 months ago) |
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look into making proper lids with filters and injection ports
but i dont see how it wouldent work
did you use a dry verm layer is the foil off now, if so did you put micro pore tape over the holes or anything
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rave420
open minded




Registered: 07/20/10
Posts: 694
Loc: Vancouver Island
Last seen: 14 years, 2 months
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Re: Help with sterilization (Cubensis) [Re: jokefox]
#13029888 - 08/10/10 12:20 AM (14 years, 5 months ago) |
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lids with filter and injection port for BRF Jars? I don't know... I prefer the vermiculite barier, they seem to colonize faster with the holes open, and i don't see the need for a filter either.
For LC and Grain jars use filtered lids with injection ports for sure. they make life a lot easier. But for standard PF Tek BRF Jars... might be overkill.
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jokefox
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Registered: 12/22/09
Posts: 6,231
Loc: never where I should be
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Re: Help with sterilization (Cubensis) [Re: rave420]
#13029903 - 08/10/10 12:24 AM (14 years, 5 months ago) |
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i do cakes for prints my jars are pre cool i need to upgrade to PP5 jars and lids with syringe filters and nice injection port trust me it sounds flashy (witch it is) but its more peace of mind that it CANT fuck up
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Doobie Scooby
Stranger

Registered: 08/09/10
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Last seen: 14 years, 4 months
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Re: Help with sterilization (Cubensis) [Re: rave420]
#13029955 - 08/10/10 12:40 AM (14 years, 5 months ago) |
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Yes i put a dry layer of verm ontop of my verm and brown rice mix, the foil is still on and they are it a very safe sterile place without micro tape also
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jokefox
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Registered: 12/22/09
Posts: 6,231
Loc: never where I should be
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Re: Help with sterilization (Cubensis) [Re: Doobie Scooby]
#13029957 - 08/10/10 12:41 AM (14 years, 5 months ago) |
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you should take off the foil
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Doobie Scooby
Stranger

Registered: 08/09/10
Posts: 19
Last seen: 14 years, 4 months
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Re: Help with sterilization (Cubensis) [Re: jokefox]
#13029963 - 08/10/10 12:43 AM (14 years, 5 months ago) |
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Will Do, may i ask why?
thanks
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jokefox
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Registered: 12/22/09
Posts: 6,231
Loc: never where I should be
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Re: Help with sterilization (Cubensis) [Re: Doobie Scooby]
#13029967 - 08/10/10 12:44 AM (14 years, 5 months ago) |
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better GE (gas exchange)
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Doobie Scooby
Stranger

Registered: 08/09/10
Posts: 19
Last seen: 14 years, 4 months
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Re: Help with sterilization (Cubensis) [Re: jokefox]
#13030001 - 08/10/10 12:53 AM (14 years, 5 months ago) |
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Ok and also, any certain temp i should keep at for mycelium colonization
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Mycjunky
Stranger


Registered: 07/25/09
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Re: Help with sterilization (Cubensis) [Re: Doobie Scooby]
#13030003 - 08/10/10 12:54 AM (14 years, 5 months ago) |
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Mid 70's is where you want to be.
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jokefox
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Registered: 12/22/09
Posts: 6,231
Loc: never where I should be
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Re: Help with sterilization (Cubensis) [Re: Mycjunky]
#13030010 - 08/10/10 12:56 AM (14 years, 5 months ago) |
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room temp
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Doobie Scooby
Stranger

Registered: 08/09/10
Posts: 19
Last seen: 14 years, 4 months
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Re: Help with sterilization (Cubensis) [Re: jokefox]
#13030030 - 08/10/10 01:01 AM (14 years, 5 months ago) |
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Thanks guys!
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