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OfflineDjango12
Spider Pants ofLance McGance

Registered: 01/20/08
Posts: 120
Last seen: 14 years, 20 days
Piece of red RTV in LC?
    #13028443 - 08/09/10 07:13 PM (14 years, 5 months ago)

So my good friend is doing a dex/malt LC per monstermitch's LC tek, with inoculation ports and whatman filters. He's done it before and had success, but hates/and is sketched out by replacing the ports when the time comes, so he decided to put dabs of RTV on the underside of the ports for extra sealing power this time.

However, he just finished innoculating them, and in one jar he spotted a tiny piece of red RTV floating around that must have come off the bottom of the port. Will this cause contamination/hurt the culture? Thanks.

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InvisibleBase Icks
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Registered: 03/19/10
Posts: 6,191
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Re: Piece of red RTV in LC? [Re: Django12]
    #13028472 - 08/09/10 07:20 PM (14 years, 5 months ago)

Silicone is inert. Thats why they use it in fake boobs.

As long as you still have a seal, you should be fine.

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OfflineDjango12
Spider Pants ofLance McGance

Registered: 01/20/08
Posts: 120
Last seen: 14 years, 20 days
Re: Piece of red RTV in LC? [Re: Base Icks]
    #13028537 - 08/09/10 07:37 PM (14 years, 5 months ago)

Thanks!

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