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OfflineHowardo
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Registered: 01/20/03
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More whining (what is causing this?)
    #1302607 - 02/12/03 02:10 PM (13 years, 9 months ago)

Ok i'm sure you heard it enough, but my jars are NOT colonizing and it's worrying me. Sorry for posting so much and i promise it's the LAST one. Now things seemed great until nothing happed. it's been a month and nothing. so i just wanted a professional (?) opinion. what is causing this? could it be contam, there's not signs of anything in them. them is at a range from 75-84 F all day.... anyone please help me out.


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Anonymous

Re: More whining (what is causing this?) [Re: Howardo]
    #1302612 - 02/12/03 02:12 PM (13 years, 9 months ago)

Where are you getting your spores?

Do you let the jars cool properly before innoculation?

Correct water content?

What do they smell like inside?


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Offlinediggitydankman
No longer aCannabisConnoisseur

Registered: 12/10/02
Posts: 479
Loc: Michgan
Last seen: 13 years, 7 months
Re: More whining (what is causing this?) [Re: Howardo]
    #1302613 - 02/12/03 02:12 PM (13 years, 9 months ago)

Sounds like the syringe was shit. There could be contam so lift off the tape and give it a whiff, if it smells bad at all they are contamed.


--------------------
"It's only wrong if you get caught.
If consequences dictate
my course of action
I should play GOD."

Maynard James Keenan, Tool


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OfflineHowardo
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Re: More whining (what is causing this?) [Re: diggitydankman]
    #1302624 - 02/12/03 02:16 PM (13 years, 9 months ago)

PF classic race from... PF. the spores seemed to be fine, little floaties and slightly viscous. but if i lift off the tape, is it possible for contams to enter therin?

EDIT: and they cooled overnight in a very cold place.. maybe too cold for innoculation. i'd say about 50-60 F...


Edited by Howardo (02/12/03 02:18 PM)


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Anonymous

Re: More whining (what is causing this?) [Re: Howardo]
    #1302629 - 02/12/03 02:18 PM (13 years, 9 months ago)

Use a rubberband and a coffee filter after you remove the tape to allow for better air exchange. In fact air exchange may be the problem.....


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Invisibledog
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Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: More whining (what is causing this?) [Re: Howardo]
    #1302636 - 02/12/03 02:20 PM (13 years, 9 months ago)

Dude, after a month your jars are fucked anyway. Open one up and take a careful sniff. Does it smell like sour vinegar?


--------------------


Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


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Offlinediggitydankman
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Re: More whining (what is causing this?) [Re: Howardo]
    #1302639 - 02/12/03 02:21 PM (13 years, 9 months ago)

If they have not grown in a month, I would say there is little to no chance that you will ever see growth from them. With that said you can check for contams by smell, if there is any smell other than normal mushroomy stench probably contams. It is possible for thermal death of spores and mycelia, but 50-60F is not going to kill them.


--------------------
"It's only wrong if you get caught.
If consequences dictate
my course of action
I should play GOD."

Maynard James Keenan, Tool


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Invisibledog
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Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: More whining (what is causing this?) [Re: ]
    #1302646 - 02/12/03 02:25 PM (13 years, 9 months ago)

Quote:

In fact air exchange may be the problem.....



Even in a sealed jar there is enough air for germination. The poster reported ZERO growth.


--------------------


Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


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OfflineHowardo
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Re: More whining (what is causing this?) [Re: dog]
    #1302672 - 02/12/03 02:33 PM (13 years, 9 months ago)

Well here's the thing one jar is like 10% colonized, if that... so i know the spores are good. i jsut can't figure out what's going on...


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Offlinediggitydankman
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Last seen: 13 years, 7 months
Re: More whining (what is causing this?) [Re: Howardo]
    #1302679 - 02/12/03 02:34 PM (13 years, 9 months ago)

Uhmmmm.... how much spore solution did you use per jar?


--------------------
"It's only wrong if you get caught.
If consequences dictate
my course of action
I should play GOD."

Maynard James Keenan, Tool


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OfflineHowardo
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Re: More whining (what is causing this?) [Re: diggitydankman]
    #1302695 - 02/12/03 02:36 PM (13 years, 9 months ago)

it was tough to evenly distribute, but i'd say 1 - 2 per jar, perhaps even as much as 2.5 cc


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OfflineAngry Mycologist
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Registered: 11/24/02
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Re: More whining (what is causing this?) [Re: Howardo]
    #1302698 - 02/12/03 02:37 PM (13 years, 9 months ago)

I agree with everyone else. Take a whiff' and tell us. That doesn't sound good at all.


--------------------
The proper penalty of ignorance, which is of course that those who don't know should learn from those who do... - Plato


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OfflineMycotopian
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Re: More whining (what is causing this?) [Re: Howardo]
    #1302719 - 02/12/03 02:41 PM (13 years, 9 months ago)

I bet it was just the syringe and you can go ahead and re-inoculate with GOOD SPORES. This has happened to me twice this month and it was just bad syringes. After a few bad ones from other places, I would now recomend TLG or Ralphsters.


--------------------
"Hockey is a sport for white men. Basketball is a sport for black men. Golf is a sport for white men dressed like black pimps."





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OfflineMycotopian
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Re: More whining (what is causing this?) [Re: dog]
    #1302730 - 02/12/03 02:43 PM (13 years, 9 months ago)

Quote:

Dude, after a month your jars are fucked anyway. Open one up and take a careful sniff. Does it smell like sour vinegar?



Why? If there is no contams, can't he just re-inject with good spores?


--------------------
"Hockey is a sport for white men. Basketball is a sport for black men. Golf is a sport for white men dressed like black pimps."





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OfflineHowardo
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Re: More whining (what is causing this?) [Re: Mycotopian]
    #1302739 - 02/12/03 02:45 PM (13 years, 9 months ago)

NO ONE SHIPS TO CALI :frown:


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Offlinediggitydankman
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Registered: 12/10/02
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Re: More whining (what is causing this?) [Re: Mycotopian]
    #1302742 - 02/12/03 02:46 PM (13 years, 9 months ago)

We are trying to determine if he has contaminated substrate by smelling for it. If he does introducing good viable spores to the substrate will likely result in more cases of little to no growth.


--------------------
"It's only wrong if you get caught.
If consequences dictate
my course of action
I should play GOD."

Maynard James Keenan, Tool


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OfflineHowardo
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Re: More whining (what is causing this?) [Re: diggitydankman]
    #1302761 - 02/12/03 02:51 PM (13 years, 9 months ago)

i'll reply tomorrow on the scent of my jars. ok? thanks for the help so far


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