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lowercaseq
Stranger
Registered: 08/02/10
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Last seen: 14 years, 5 months
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something doesnt look right . anyone please please help
#13021067 - 08/08/10 10:18 AM (14 years, 5 months ago) |
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So everything went good birthing and whatnot . let them soak and then rolled them in vermiculite . then I have them in a container with perlite in the bottom . about 4 inches.. I have numerous holes in the container . that was on . wednesday . and on thursday I already had one muchroom shoot up . and its growing . its prolly about an inch now and thick . looks good . but none of the others have any signs of anything .. ? is it because they are waiting to fully colonize and I birthed them a little early perhaps?
any encouraging thoughts would be nice thanks
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mellowparty
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Registered: 05/17/09
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Re: something doesnt look right . anyone please please help [Re: lowercaseq]
#13021087 - 08/08/10 10:23 AM (14 years, 5 months ago) |
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Did you wait for the one week consolidation period?
Just give it more time man. It usually takes me about a week to see the first pins. But I never do cakes (only casings) so just give them more time and more FAE (+adequate misting).
Pics would be lovely
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blueshroomy88
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Registered: 12/15/08
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Re: something doesnt look right . anyone please please help [Re: lowercaseq]
#13021098 - 08/08/10 10:26 AM (14 years, 5 months ago) |
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It's difficult to say without pictures, but I would research pinning triggers. Even though you do have one growing, your set up may be lacking in one of the pinning trigger areas.
As I recall, Fresh Air Exchange, Lighting, Humidity, etc. are a few of the pinning triggers. These are not in order of importance. Just saying.
Or give it time, if you've got one showing, maybe a day or two from now, you'll have several showing. Just a thought.
-blue
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mellowparty
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Registered: 05/17/09
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Re: something doesnt look right . anyone please please help [Re: blueshroomy88]
#13021125 - 08/08/10 10:36 AM (14 years, 5 months ago) |
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Quote:
pinning triggers
You nailed the majority of such triggers but I just wanted to emphasize on the importance of low wavelength irradiation. UV rays are very effective for pin inducement.
If you have a UV source just use it for a couple of secs, several times a day and you'll see pins in no time
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: something doesnt look right . anyone please please help [Re: mellowparty]
#13021133 - 08/08/10 10:39 AM (14 years, 5 months ago) |
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Quote:
mellowparty said: If you have a UV source
UV light causes cataracts, mutations, cancer, blindness, death. Please don't use UV in mycology.
-------------------- You make it all possible. Doesn't it feel good?
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Base Icks



Registered: 03/19/10
Posts: 6,191
Loc: Shroomshire
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Re: something doesnt look right . anyone please please help [Re: Doc_T]
#13021139 - 08/08/10 10:40 AM (14 years, 5 months ago) |
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Patience.
If you used a spore syringe, it can take up to two weeks.
Are you misting?
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anathema
pǝsnɟuoƆ



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Re: something doesnt look right . anyone please please help [Re: blueshroomy88]
#13021176 - 08/08/10 10:50 AM (14 years, 5 months ago) |
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For pinning triggers from RR's notes:
*The major pinning triggers are in order of importance, full colonization, a decrease in CO2 levels due to increased air exchange (not gas exchange which is minimal), a steady rate of evaporation from the substrate or casing layer, and lastly, light. Hyphal knots form best in 100% humidity, but I didn't list that because it's not a pinning trigger, but rather an environmental condition that is necessary."
-------------------- --
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mellowparty
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Re: something doesnt look right . anyone please please help [Re: Doc_T]
#13021184 - 08/08/10 10:52 AM (14 years, 5 months ago) |
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Quote:
Doc_T said:
Quote:
mellowparty said: If you have a UV source
UV light causes cataracts, mutations, cancer, blindness, death. Please don't use UV in mycology.
1-2 seconds exposure of the UV onto the myc won't hurt u man.
It will stimulate the differentiation of pins but not necessarily cancer and all that shit you listed.
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mellowparty
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Re: something doesnt look right . anyone please please help [Re: mellowparty]
#13021187 - 08/08/10 10:54 AM (14 years, 5 months ago) |
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And besides a UV lamp is AWESOME for sterilisation.
I was using my weed growing lamp to sterilise petri dishes with agar and I achieved 100% efficiency 
I say use UV in mycology. It works 
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: something doesnt look right . anyone please please help [Re: mellowparty]
#13021215 - 08/08/10 10:59 AM (14 years, 5 months ago) |
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Quote:
mellowparty said: 1-2 seconds exposure of the UV onto the myc won't hurt u man.
Exposure is cumulative over your entire life. UV sterilizes by destroying living cells. It's that easy.
Do as you wish, but I choose SPF 30.
-------------------- You make it all possible. Doesn't it feel good?
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rave420
open minded




Registered: 07/20/10
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Loc: Vancouver Island
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Re: something doesnt look right . anyone please please help [Re: mellowparty]
#13021218 - 08/08/10 11:00 AM (14 years, 5 months ago) |
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Quote:
mellowparty said:
Quote:
Doc_T said:
Quote:
mellowparty said: If you have a UV source
UV light causes cataracts, mutations, cancer, blindness, death. Please don't use UV in mycology.
1-2 seconds exposure of the UV onto the myc won't hurt u man.
It will stimulate the differentiation of pins but not necessarily cancer and all that shit you listed.
you wouldn't happen to have any factual information as to why something like that might happen? Sounds interesting, but i'd like to read more before acting on anything. I have always read that UV light is a mutagen to any microorganism really.
--------------------
we all breathe the same air, drink the same water, and draw our strength from the same giant fireball.
Help me with my collection (trades only)
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mellowparty
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Re: something doesnt look right . anyone please please help [Re: Doc_T]
#13021246 - 08/08/10 11:06 AM (14 years, 5 months ago) |
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Quote:
Exposure is cumulative over your entire life.
That is true. All mutations accumulate during your life time and once a certain quota of cell cycle regulation genes are affected you get cancer. However, when you expose the cakes to UV you WILL take some precautions not to get scorched. Probably a pair of those special orange glasses would come in handy. But for pinning inducement only I guess a diffused sun light would do the trick as well.
Quote:
UV sterilizes by destroying living cells. It's that easy
It damages adjacent thymidine base pairs by forming a cyclobutyl ring between them. Also I guess it generates some vile radicals that also damage DNA. Its great for sterilisation of surfaces. Seriously its the most effective way to make 100% sterile agar dishes (at least for me).
No need to diss UV that quickly. With a bit of precaution you can gain a lot from it 
People at microbiology labs use it all the time.
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mellowparty
legitimate researcher


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Re: something doesnt look right . anyone please please help [Re: rave420]
#13021284 - 08/08/10 11:16 AM (14 years, 5 months ago) |
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Quote:
rave420 said:
Quote:
mellowparty said:
Quote:
Doc_T said:
Quote:
mellowparty said: If you have a UV source
UV light causes cataracts, mutations, cancer, blindness, death. Please don't use UV in mycology.
1-2 seconds exposure of the UV onto the myc won't hurt u man.
It will stimulate the differentiation of pins but not necessarily cancer and all that shit you listed.
you wouldn't happen to have any factual information as to why something like that might happen? Sounds interesting, but i'd like to read more before acting on anything. I have always read that UV light is a mutagen to any microorganism really.
No. I'm not aware of the molecular mechanism of how that happens.
But it seems logical that the myc would want to make a frut body once the substrate surface has been reached. What better way to decide than monitoring for light and fresh air.
That actually got my curiosity as well. I think I'll research it.
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mellowparty
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Re: something doesnt look right . anyone please please help [Re: mellowparty]
#13021417 - 08/08/10 11:55 AM (14 years, 5 months ago) |
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I didn't find much but here is something that is good to know:
Quote:
Light and wavelength for inoculating, incubating, casing, pining and fruiting:
■One of the most important factors in yield is a strong and full primordial formation period and pinhead set[1]
■Blue and UVa wavelengths offer the most abundant pinhead set with peaks at the nanometers of 370, 440 and 460[2].
■UVb (290–320 nm) light is much less powerful when the source (bulb) is greater than 12" form the destination (cropping surface). UVa (315-400 nm) light is stronger at greater distances. For a source of UVa I will use "ZooMed" "Reptisun 5.0 UVb"[3].
■The best source of light for pining and fruiting is natural wavelengths from fluorescent lights. But the photo-requirements of p.cube calls for "at least 140 microwatts per 10 nanometer per lumen of blue spectra (440-495 nm)"[4], and neither cool-white or warm-white offer that blue light[4]. Commercial light bulbs from "Duro-Test Lighting, Inc"[5] such as "Vita Lite" and "Daylight 65" offer the required blue spectra for p.cubes[4].
■Fluorescent should be kept at least 18" from the cropping surface[4]
■Red and green light with nanometer greater than 510 are ineffective (eg. invisible) to p.cube growth[2] and can be used when working with inoculation, incubation, spawn, bulk substrate, casing layers, patch casing, etc. Im pretty sure this is supposed to increase speed and aggressiveness of the inoculation, colonization of substrates, casing, etc...
From the articles I read it seems that the blue to UV-A spectrum is sensed by a special photoreceptor that contains a long unsaturated hydrocarbon chains. I suspect that this is a 7-transmembrane receptor that transmits the quantum signal to the activation of G protein (we detect light this way too). The activated G-protein in turn stimulates the activity of adenylyl cyclase which produces cyclic adenosine monophosphate (cAMP) from adenosine triphosphate (ATP). The onset of pinning comes with an increase in the levels of cellular cyclic AMP levels which are required for the formation of a knot.
Here you can see how cAMP attracts a bunch of fungal-like (but no fungal) cells.
This leads to the formation of a fruting structure in this particular organism (much like in our beloved mushrooms )

Appart from causing cellular aggregation (thats a short-term response) cAMP levels affect gene transcription rates (thats the long-term response). Such a change in the transcriptional output of the myc could lead to its proliferation and thus the formation of a pinhead and thus a young mushroom.

I hope you find this helpful and interesting
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Javadog
Continuing along



Registered: 05/03/10
Posts: 7,385
Loc: USA
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Re: something doesnt look right . anyone please please help [Re: mellowparty]
#13021847 - 08/08/10 01:58 PM (14 years, 5 months ago) |
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Whooosh! Great stuff.
Now, if I can only figure out where the HydroEncabulator goes, then I'll be all set. ;0)
I will probably be looking this post up in a few years too.
JD
-------------------- Boyd Rice told my brother that life is a corny pack of freesakes
Myco-tek.org
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