Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Liquid Cultures   Myyco.com Golden Teacher Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinelastlokean
Stranger
 User Gallery
Registered: 07/30/07
Posts: 106
Last seen: 11 years, 10 months
LC always fails, agar to grain works fine WTF?
    #13017272 - 08/07/10 01:51 PM (14 years, 5 months ago)

So... awhile back the green meanies hit hard, failing multiple monotubs!

Sense then the decision to return to a martha for smaller controlled cakes was made.  Sense then success has been limited, with a seemingly endless trich problem.

Currently spores -> Agar -> Agar -> Agar -> LC -> Grain -> Bulk is being utilized to get clean healthy culture. (In theory)

With about a 90% contaminate rate using good sterile procedures in a glove box.  Originally the blame was cast onto sterile process.  And now it is way overkill, and only FRESH brand new syringes jars and all equipment are used.  (Just out of extreme paranoia)

After an entire month of failures a change was made to straight agar transfers to grain.  This has found a much higher success rate close to 100%.

Whats interesting is the LC would look fine, test fine on agar and look  fine when colonized on grain about half the time and green the other half.  Any of the grain that looked fine would contam instantly when spawned to bulk.

Years prior have had no problems with LC and I can't seem to figure out whats wrong with the LC.

Using sugar based LCs at anywhere from 2-4% solution of turbinado or honey using distilled water.  They are PC'd for half an hour to 45 minutes at 15 psi.  Always wrapped in foil, sometimes removed while still warm for filter to dry fast, sometimes let cool in the PC for 24 hours.  (Not sure which is 'correct')

For a LC lid two holes are drilled. One for 2 pieces of micropore tape and one for a silicon injection port.  The holes are always sanded down  very well till smooth.


Anyone figure why the LCs would consistently FAIL?


--------------------

Recipe:
How a Lokean makes chocolates

For a fully enclosed gh with a coolmist piped in, one may find that adding one of these to the opposite end as the intake will seriously help reduce pressure buildup and strain on the poor weak coolmist motor, while also dramatically improving fae without cutting polyfill stuffed slits all over the damn thing.  (That looks suspicious :smirk:)

Extras: Filter Print Post Top
Invisiblesandman420
Saint PP
Male


Registered: 06/17/04
Posts: 5,387
Trusted Cultivator
Re: LC always fails, agar to grain works fine WTF? [Re: lastlokean]
    #13017873 - 08/07/10 03:59 PM (14 years, 5 months ago)

You could try PCing for less time. 20 minutes is fine at 15 psi. You dont ever want to exceed 15 psi even by a little with an LC.

Micropore tape is a poor choice in filter material for LCs. You would be better off with one of those rigs with the flexible tubing stuffed with polyfil in the lid and a single rtv port. This way you can pinch the tube off with a clip or something and shake the jar very well every day or two to speed up growth and get a dense culture, which you cant do with a micropore taped up hole. Plus micropore tape has got to have some huge micron spaces. I would just wildly guess by eybelalling it that it has to be in the 50-100 micron range or even higher. You can literally see gaps you know.

Are you trying Agar to LC Or spore to LC?

All in all LC is an art form, kinda like being a good chef. It takes some practice for some situations and some people to excel at it.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

Extras: Filter Print Post Top
Invisiblesandman420
Saint PP
Male


Registered: 06/17/04
Posts: 5,387
Trusted Cultivator
Re: LC always fails, agar to grain works fine WTF? [Re: sandman420]
    #13017889 - 08/07/10 04:02 PM (14 years, 5 months ago)

or you could just do what youre doing, and then use the grain to make a "Grain LC" by injecting sterile water and shaking the jar up.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

Extras: Filter Print Post Top
InvisibleMrPinkFloyd


Registered: 07/13/09
Posts: 683
Loc: United States Flag
Re: LC always fails, agar to grain works fine WTF? [Re: sandman420]
    #13018171 - 08/07/10 05:13 PM (14 years, 5 months ago)

I think it's the micropore tape smuggling in the nasties.  :ninjalike:

Extras: Filter Print Post Top
Offlinelastlokean
Stranger
 User Gallery
Registered: 07/30/07
Posts: 106
Last seen: 11 years, 10 months
Re: LC always fails, agar to grain works fine WTF? [Re: MrPinkFloyd]
    #13018269 - 08/07/10 05:40 PM (14 years, 5 months ago)

Thanks for the insight, will give it a fresh try with a more proper filter!


--------------------

Recipe:
How a Lokean makes chocolates

For a fully enclosed gh with a coolmist piped in, one may find that adding one of these to the opposite end as the intake will seriously help reduce pressure buildup and strain on the poor weak coolmist motor, while also dramatically improving fae without cutting polyfill stuffed slits all over the damn thing.  (That looks suspicious :smirk:)

Extras: Filter Print Post Top
InvisibleJavadog
Continuing along
Male User Gallery


Registered: 05/03/10
Posts: 7,385
Loc: USA Flag
Trusted Cultivator
Re: LC always fails, agar to grain works fine WTF? [Re: lastlokean]
    #13018617 - 08/07/10 07:06 PM (14 years, 5 months ago)

Quote:

lastlokean said:
So... awhile back the green meanies hit hard, failing multiple monotubs!

Sense then the decision to return to a martha for smaller controlled cakes was made.  Sense then success has been limited, with a seemingly endless trich problem.

Currently spores -> Agar -> Agar -> Agar -> LC -> Grain -> Bulk is being utilized to get clean healthy culture. (In theory)

With about a 90% contaminate rate using good sterile procedures in a glove box.  Originally the blame was cast onto sterile process.  And now it is way overkill, and only FRESH brand new syringes jars and all equipment are used.  (Just out of extreme paranoia)

After an entire month of failures a change was made to straight agar transfers to grain.  This has found a much higher success rate close to 100%.

Whats interesting is the LC would look fine, test fine on agar and look  fine when colonized on grain about half the time and green the other half.  Any of the grain that looked fine would contam instantly when spawned to bulk.

Years prior have had no problems with LC and I can't seem to figure out whats wrong with the LC.

Using sugar based LCs at anywhere from 2-4% solution of turbinado or honey using distilled water.  They are PC'd for half an hour to 45 minutes at 15 psi.  Always wrapped in foil, sometimes removed while still warm for filter to dry fast, sometimes let cool in the PC for 24 hours.  (Not sure which is 'correct')

For a LC lid two holes are drilled. One for 2 pieces of micropore tape and one for a silicon injection port.  The holes are always sanded down  very well till smooth.


Anyone figure why the LCs would consistently FAIL?




I have posted about sudden and consistent problems in making
LCs for three new stain syringes.

I think that I may have figured out why by testing on agar though,
as I am seeing a weak bacterial infection in each syringes petri dish.

Good luck!

JD


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org

Extras: Filter Print Post Top
Offlinelastlokean
Stranger
 User Gallery
Registered: 07/30/07
Posts: 106
Last seen: 11 years, 10 months
Re: LC always fails, agar to grain works fine WTF? [Re: Javadog]
    #13018714 - 08/07/10 07:34 PM (14 years, 5 months ago)

I'm getting trich. not a weak bacterial infection...


--------------------

Recipe:
How a Lokean makes chocolates

For a fully enclosed gh with a coolmist piped in, one may find that adding one of these to the opposite end as the intake will seriously help reduce pressure buildup and strain on the poor weak coolmist motor, while also dramatically improving fae without cutting polyfill stuffed slits all over the damn thing.  (That looks suspicious :smirk:)

Extras: Filter Print Post Top
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: LC always fails, agar to grain works fine WTF? [Re: lastlokean]
    #13018807 - 08/07/10 07:56 PM (14 years, 5 months ago)

LC w/ hidden trich. :shrug: Happens.
If you look carefully, there's probably a green grain in each fully white jar.


--------------------
You make it all possible. Doesn't it feel good?

Extras: Filter Print Post Top
Offlinelastlokean
Stranger
 User Gallery
Registered: 07/30/07
Posts: 106
Last seen: 11 years, 10 months
Re: LC always fails, agar to grain works fine WTF? [Re: Doc_T]
    #13019176 - 08/07/10 09:26 PM (14 years, 5 months ago)

But hidden green shouldn't happen to 30+ LC in a row made from different strains.  I've decided not to continue trusting micropore tape on the LC lids anymore...  Instead gonna use the auto tubing + poly-fill. Hopefully this fixes the problem!


--------------------

Recipe:
How a Lokean makes chocolates

For a fully enclosed gh with a coolmist piped in, one may find that adding one of these to the opposite end as the intake will seriously help reduce pressure buildup and strain on the poor weak coolmist motor, while also dramatically improving fae without cutting polyfill stuffed slits all over the damn thing.  (That looks suspicious :smirk:)

Extras: Filter Print Post Top
Invisiblesandman420
Saint PP
Male


Registered: 06/17/04
Posts: 5,387
Trusted Cultivator
Re: LC always fails, agar to grain works fine WTF? [Re: lastlokean]
    #13019184 - 08/07/10 09:28 PM (14 years, 5 months ago)

I think that will do you good my friend.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Liquid Cultures   Myyco.com Golden Teacher Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate


Similar ThreadsPosterViewsRepliesLast post
* Benefits of Agar? celsius 9,660 10 12/01/19 12:36 AM
by wildernessjunkie
* Will my agar still work? Dr_Smegma 172 4 03/07/18 03:59 AM
by Bensi
* Cranberry juice extraction from rye grain works!!! EPerson100 8,397 4 02/12/02 03:13 PM
by CuckoosNest
* PF-Tek or Rye grain DrD525 11,359 17 06/03/16 01:57 PM
by openmindedcanvas
* DAMN THIS GRAIN! andrewcubensis 1,310 6 07/08/02 06:44 AM
by whackedout
* Re: How do you prepare whole grain rye?? Lana 2,524 1 07/31/00 07:54 PM
by Anonymous
* spore life and inoculating agar JonnyOnTheSpot 1,175 1 01/31/02 05:45 PM
by djveiox
* whole grain brown rice fuzzysquirelnuts 2,379 6 08/20/01 06:13 PM
by L3D

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,162 topic views. 19 members, 98 guests and 76 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.019 seconds spending 0.004 seconds on 12 queries.