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ThreeShoes
Spiritual adventurer


Registered: 08/07/10
Posts: 96
Loc: Nibiru
Last seen: 14 years, 1 month
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I made a dumb mistake. Advice needed.
#13018556 - 08/07/10 06:54 PM (14 years, 5 months ago) |
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I recently started trying to grow my own mushrooms. Yesterday after sterilizing my jars I almost immediately started inoculating them. I then realized this was a terrible idea and I probably killed my spores due to the heat. My question to you is this, since my jars have now cooled down would it be ok to try inoculating them again if I use good sterilization techniques?
-------------------- Humans are just a complicated form of bacteria - Joe Rogan
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jokefox
Top of the chain



Registered: 12/22/09
Posts: 6,231
Loc: never where I should be
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Re: I made a dumb mistake. Advice needed. [Re: ThreeShoes]
#13018562 - 08/07/10 06:55 PM (14 years, 5 months ago) |
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it depends on how much you knocked them up if you use to much liquid then its just going to mess up the moisture ratio and make it easier for bacteria to take over
plus mycelium likes dryer substrate when its trying to colonize it
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ThreeShoes
Spiritual adventurer


Registered: 08/07/10
Posts: 96
Loc: Nibiru
Last seen: 14 years, 1 month
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Re: I made a dumb mistake. Advice needed. [Re: jokefox]
#13018594 - 08/07/10 07:02 PM (14 years, 5 months ago) |
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I probably used less than a CC. Is it worth a shot?
-------------------- Humans are just a complicated form of bacteria - Joe Rogan
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Naz
Fungal Infection



Registered: 12/14/09
Posts: 917
Last seen: 10 years, 1 month
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Re: I made a dumb mistake. Advice needed. [Re: ThreeShoes]
#13018609 - 08/07/10 07:04 PM (14 years, 5 months ago) |
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would it really be a big deal to make up new jars...it will prolly be the best choice. would suck to have contam show up 2 weeks in and have to start over then.
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ThreeShoes
Spiritual adventurer


Registered: 08/07/10
Posts: 96
Loc: Nibiru
Last seen: 14 years, 1 month
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Re: I made a dumb mistake. Advice needed. [Re: Naz]
#13018630 - 08/07/10 07:09 PM (14 years, 5 months ago) |
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Quote:
Naz said: would it really be a big deal to make up new jars...it will prolly be the best choice. would suck to have contam show up 2 weeks in and have to start over then.
I only have about 2cc's of spores left and maybe one cup of BRF, and money is kind of tight. You make a could point though. Thanks for the advice. Is there any way I can close this thread? Sorry, I'm new here.
-------------------- Humans are just a complicated form of bacteria - Joe Rogan
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irockesp
Seeker

Registered: 06/08/10
Posts: 171
Last seen: 13 years, 2 months
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Re: I made a dumb mistake. Advice needed. [Re: ThreeShoes]
#13018764 - 08/07/10 07:46 PM (14 years, 5 months ago) |
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Quote:
ThreeShoes said:
Quote:
Naz said: would it really be a big deal to make up new jars...it will prolly be the best choice. would suck to have contam show up 2 weeks in and have to start over then.
I only have about 2cc's of spores left and maybe one cup of BRF, and money is kind of tight. You make a could point though. Thanks for the advice. Is there any way I can close this thread? Sorry, I'm new here.
In this case I would do some agar work. Only takes a drop to get started
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Teezy
Nub


Registered: 07/09/10
Posts: 137
Last seen: 12 years, 8 months
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Re: I made a dumb mistake. Advice needed. [Re: irockesp]
#13018789 - 08/07/10 07:53 PM (14 years, 5 months ago) |
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The same thing happened to me a little over a month ago. I knocked my jars up probaly an hour or 2 after i pc. From what others have told me, yes, you can knock them up again if you wish. I just let mine chill for a week or 2 just to make sure. Come to find out i did indeed ruin all my jars but i also had one that grew and its in the fc as we speak. Its up to you what you wanna do, i would wait and see and maybe make a few new jars also. Or knock them up again using very little spore solution.
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Naz
Fungal Infection



Registered: 12/14/09
Posts: 917
Last seen: 10 years, 1 month
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Re: I made a dumb mistake. Advice needed. [Re: Teezy]
#13018890 - 08/07/10 08:19 PM (14 years, 5 months ago) |
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if you have a pc, get like 3 dollar wbs bag from wallie mart, a couple cc's can make a whole lotta mycelium water
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