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Offlineinsutama
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First spore print/syringe epic fail
    #13016372 - 08/07/10 09:53 AM (14 years, 5 months ago)

Well so with this been my third grow i thought it was time to become self sufficient so i tried taking a spore print and making a syringe well let just say FAIL lol. So like i said in a previous thread my spore prints came out really faint. So in my glove box i have my syringe,spore print, shotglass, spore loop, and paper towel soaking in alcohol. First i unwrap the spore print and start trying to scrape spores into the shotglass, there was no way any spores were going into the shot glass so in the end i had to use my scalpel to scrape the spores into the glass then when i opened up the tinfoil around my spore syringe i found out i had like 2cc's of water left in it (i guess PCing the syringe took the water out of it). So heres my questions
1) How do you get the spore off the tinfoil without scraping the foil with a scalpel ?
2) How do you keep the water in the syringe when PCing ? Should i maybe just pc a empty syringe then do the boiling water method to refill the syringe ?
3) lastly do you see anything else i am doing wrong ? I may have a other shot to take a spore print with my next flush and i dont wanna mess it up

Thanks in advance for any advice and sorry about just a long post

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OfflineeVenom
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Re: First spore print/syringe epic fail [Re: insutama]
    #13016397 - 08/07/10 10:08 AM (14 years, 5 months ago)

(Warning):trippinballs:

1 you sqwirt water from the syringe to the print then rub ti adn suck it backup to the syringe

2 I alwasys use the boiling water syringe sterilization tek ( look it up to wasted to explain)
:psychsplit:


--------------------
eVenom

"Some of us like a nice buzz, while others want to climb a screaming volcano naked to meet god."
RR

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InvisibleDoc_T
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Re: First spore print/syringe epic fail [Re: insutama]
    #13016430 - 08/07/10 10:16 AM (14 years, 5 months ago)

Quote:

eVenom said:
1 you sqwirt water from the syringe to the print then rub ti adn suck it backup to the syringe




I do that inside the baggie the print comes in.


Quote:

insutama said:
2) How do you keep the water in the syringe when PCing ? Should i maybe just pc a empty syringe then do the boiling water method to refill the syringe ?




Half water, half air, needle up.
Or make a jar of sterile water and sterilize empty syringes.


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You make it all possible. Doesn't it feel good?

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OfflineMad_Hatter2004
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Re: First spore print/syringe epic fail [Re: eVenom]
    #13016439 - 08/07/10 10:18 AM (14 years, 5 months ago)

Placethe tin foil with the print on it in the bottom of a clean glass jar (1/2 pint wide mouths work great)squirt some sterile water over it from a syringe and use your inoculation loop to rub it around,suck it back into the syringe.

Use the boiling water syringe method like eVenom said.

Simply bring some water to a full rolling boil,Suck up 10cc's (or however many cc's you need) and squirt back out,then suck some back up and squirt it back out...repeat this process a few times and then finally suck the boiling water back into the syringe one last time and don't squirt it back out.


Now you have a sterile syringe with sterile water.


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InvisibleDoc_T
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Re: First spore print/syringe epic fail [Re: Mad_Hatter2004]
    #13016443 - 08/07/10 10:20 AM (14 years, 5 months ago)

Lots of people use that method. With success.
But I've never understood why anybody with a PC would choose not to use it for sterilization.


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You make it all possible. Doesn't it feel good?

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Invisiblemen
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Registered: 04/25/10
Posts: 118
Re: First spore print/syringe epic fail [Re: eVenom]
    #13016453 - 08/07/10 10:24 AM (14 years, 5 months ago)

Innoculating loop - use that. Put a little water onthe print then scrape it with the innoculating loop and dip it back into ur water receptacle. Repeat and draw the spore water from ur receptacle withyour syringe.

Don't substitute the innoculating loop with sharp objects like blades or needles as they will damage the spore

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Offlineinsutama
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Re: First spore print/syringe epic fail [Re: men]
    #13016481 - 08/07/10 10:34 AM (14 years, 5 months ago)

okay great advice thanks well i just got called to work so ill be back in a day or so to try again

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