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OfflineKryptic
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Some advice, what would you guys do?
    #13014850 - 08/06/10 09:57 PM (14 years, 5 months ago)

I knocked up a bunch of quart jars a couple of weeks ago.  They all took off at the same time, they were all ready for their first shake within 1 day of each other then, it's like the slower ones over took the quicker ones and the ones that started colonizing the fastest are stalling.  The pic bellow shows two such jars the one that's almost fully colonized was way behind the one next to it.  Now out of 10 jars 3 or 4 are really ready to go and the others have really slowed down.

I took the fastest and did a G2G transfer with it so I don't have to worry about that one right now.  My question is what do you guys do when you have jars so staggered?  I had planned on doing just 2 monotubs, 5 jars of spawn each but I'm not sure if I can wait for the slower ones to catch up.

Also the 3 0r 4 jars that are ready are from 2 different strains so I dont want them in a tub together.  Thanks.



--------------------
   

Dunking the grains: results and discussion | Grain to grain (G2G) in a SAB | Elementary Coir tek | Frank's proper pasteurization tek | Frank's simple coir\verm prep | RR notes | How to dial in your monotubs like a boss. | Pastywhyte's Easy Agar Tek | Frank's agar media journal

"Walk tall, kick ass, learn to speak Arabic, love music and never forget you come from a long line of truth-seekers, lovers and warriors" -Hunter S. Thompson

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Re: Some advice, what would you guys do? [Re: Kryptic]
    #13014862 - 08/06/10 10:00 PM (14 years, 5 months ago)

Your picture is not clear, but the jar on the right looks
kinda funky. Does it smell alright?

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OfflineKryptic
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Re: Some advice, what would you guys do? [Re: stonesun]
    #13014886 - 08/06/10 10:08 PM (14 years, 5 months ago)

They look good.  Here's a better pic with some of the other slow pokes.



--------------------
   

Dunking the grains: results and discussion | Grain to grain (G2G) in a SAB | Elementary Coir tek | Frank's proper pasteurization tek | Frank's simple coir\verm prep | RR notes | How to dial in your monotubs like a boss. | Pastywhyte's Easy Agar Tek | Frank's agar media journal

"Walk tall, kick ass, learn to speak Arabic, love music and never forget you come from a long line of truth-seekers, lovers and warriors" -Hunter S. Thompson

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Re: Some advice, what would you guys do? [Re: Kryptic]
    #13014925 - 08/06/10 10:21 PM (14 years, 5 months ago)

How old are those jars?

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Re: Some advice, what would you guys do? [Re: Kryptic]
    #13014945 - 08/06/10 10:25 PM (14 years, 5 months ago)

You might have shaken too vigorously / early in the jars that stalled. I would wait it out. Knock up some more jars in the mean time.

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Re: Some advice, what would you guys do? [Re: stonesun]
    #13014960 - 08/06/10 10:27 PM (14 years, 5 months ago)

I just checked my log and their actually older then I thought.  They were all started on 07/15.


--------------------
   

Dunking the grains: results and discussion | Grain to grain (G2G) in a SAB | Elementary Coir tek | Frank's proper pasteurization tek | Frank's simple coir\verm prep | RR notes | How to dial in your monotubs like a boss. | Pastywhyte's Easy Agar Tek | Frank's agar media journal

"Walk tall, kick ass, learn to speak Arabic, love music and never forget you come from a long line of truth-seekers, lovers and warriors" -Hunter S. Thompson

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OfflineKryptic
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Re: Some advice, what would you guys do? [Re: LateForTheFuture]
    #13014970 - 08/06/10 10:31 PM (14 years, 5 months ago)

Quote:

LateForTheFuture said:
You might have shaken too vigorously / early in the jars that stalled. I would wait it out. Knock up some more jars in the mean time.





This sounds possible. 

I have some prints I could use to knock up a few more jars but if I use any more of these jars for transfers it will really change my original plan.


--------------------
   

Dunking the grains: results and discussion | Grain to grain (G2G) in a SAB | Elementary Coir tek | Frank's proper pasteurization tek | Frank's simple coir\verm prep | RR notes | How to dial in your monotubs like a boss. | Pastywhyte's Easy Agar Tek | Frank's agar media journal

"Walk tall, kick ass, learn to speak Arabic, love music and never forget you come from a long line of truth-seekers, lovers and warriors" -Hunter S. Thompson

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Re: Some advice, what would you guys do? [Re: Kryptic]
    #13015017 - 08/06/10 10:45 PM (14 years, 5 months ago)

The grains in those jars look slimey.
It is likely bacterial contamination.
That's why I asked if they smell.
What filter are you using?

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OfflineKryptic
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Re: Some advice, what would you guys do? [Re: stonesun]
    #13015100 - 08/06/10 11:09 PM (14 years, 5 months ago)

I can't smell anything through the filter and I don't want to open them.  The one I cracked for the transfer smelled like sweet fresh mushie goodness but it was like 90-95% colonized.  I'm using pieces of those filters you buy at the on-line myco shops, here's a pic of the lids I made. 



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Dunking the grains: results and discussion | Grain to grain (G2G) in a SAB | Elementary Coir tek | Frank's proper pasteurization tek | Frank's simple coir\verm prep | RR notes | How to dial in your monotubs like a boss. | Pastywhyte's Easy Agar Tek | Frank's agar media journal

"Walk tall, kick ass, learn to speak Arabic, love music and never forget you come from a long line of truth-seekers, lovers and warriors" -Hunter S. Thompson

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Re: Some advice, what would you guys do? [Re: Kryptic]
    #13015163 - 08/06/10 11:26 PM (14 years, 5 months ago)

Quote:

My question is what do you guys do when you have jars so staggered?  I had planned on doing just 2 monotubs, 5 jars of spawn each but I'm not sure if I can wait for the slower ones to catch up.



I Wait.  You're gonna have to wait.  Why are you not sure if you can wait?

Quote:

They look good.




The moisture content of those jars is too high.

Quote:

I just checked my log and their actually older then I thought.  They were all started on 07/15.




Those jars have been stalled for 3 weeks?  :facepalm:  Those jars are done for.

Quote:

I'm using pieces of those filters you buy at the on-line myco shops




Synthetic filter disks.  Your lids look nice.  The one thing I would question is those coffee filters over you lids cutting off GE and moisture evaporation.  Synthetic filter disks are plenty of filter to protect your spawn against contaminates.
HL


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How I Get Stuff done. - My Reference Guide. - My Grows.

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Re: Some advice, what would you guys do? [Re: hamloaf]
    #13015212 - 08/06/10 11:42 PM (14 years, 5 months ago)

Quote:

hamloaf said:
The moisture content of those jars is too high.





That's the sign of wetspot/bacteria/bacillus however you want to call it.

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OfflineKryptic
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Re: Some advice, what would you guys do? [Re: hamloaf]
    #13015241 - 08/06/10 11:51 PM (14 years, 5 months ago)

Quote:

hamloaf said:
Why are you not sure if you can wait?




I can wait but the jars that are at 100% need to go into fruiting now don't they? 

Quote:

The moisture content of those jars is too high. Those jars are done for.




Think I should get rid of them?

I'll lose the coffee filters on all the jars now.


--------------------
   

Dunking the grains: results and discussion | Grain to grain (G2G) in a SAB | Elementary Coir tek | Frank's proper pasteurization tek | Frank's simple coir\verm prep | RR notes | How to dial in your monotubs like a boss. | Pastywhyte's Easy Agar Tek | Frank's agar media journal

"Walk tall, kick ass, learn to speak Arabic, love music and never forget you come from a long line of truth-seekers, lovers and warriors" -Hunter S. Thompson

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Re: Some advice, what would you guys do? [Re: Kryptic]
    #13015276 - 08/07/10 12:00 AM (14 years, 5 months ago)

Spawn to bulk or case the jars that are fully colonized, now.  I am sorry but those other jars are over, toss them.  Next time loose the coffee filters.  Synthetic filter disks alone work great and are ideal for filtered GE.
HL


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How I Get Stuff done. - My Reference Guide. - My Grows.

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OfflineKryptic
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Re: Some advice, what would you guys do? [Re: Kryptic]
    #13015285 - 08/07/10 12:03 AM (14 years, 5 months ago)

I looked at all the jars again very close.  OK so I guess they don't all look so good.  There is definitely signs of moisture in most of the jars. 1 or 2 jars have a very very small amount of yellowish liquid in the bottom of the jar.

I have some jars that are fully colonized so I should be able to spawn those.  Not sure how my transfer will work out but I have that.

This is my 2nd grow and my 1st rye grow, I'd like to know where I went wrong.


--------------------
   

Dunking the grains: results and discussion | Grain to grain (G2G) in a SAB | Elementary Coir tek | Frank's proper pasteurization tek | Frank's simple coir\verm prep | RR notes | How to dial in your monotubs like a boss. | Pastywhyte's Easy Agar Tek | Frank's agar media journal

"Walk tall, kick ass, learn to speak Arabic, love music and never forget you come from a long line of truth-seekers, lovers and warriors" -Hunter S. Thompson

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OfflineKryptic
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Re: Some advice, what would you guys do? [Re: hamloaf]
    #13015289 - 08/07/10 12:04 AM (14 years, 5 months ago)

Quote:

hamloaf said:
Spawn to bulk or case the jars that are fully colonized, now.  I am sorry but those other jars are over, toss them.  Next time loose the coffee filters.  Synthetic filter disks alone work great and are ideal for filtered GE.
HL





Saw this after I posted above.  Ok, thanks.


--------------------
   

Dunking the grains: results and discussion | Grain to grain (G2G) in a SAB | Elementary Coir tek | Frank's proper pasteurization tek | Frank's simple coir\verm prep | RR notes | How to dial in your monotubs like a boss. | Pastywhyte's Easy Agar Tek | Frank's agar media journal

"Walk tall, kick ass, learn to speak Arabic, love music and never forget you come from a long line of truth-seekers, lovers and warriors" -Hunter S. Thompson

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Re: Some advice, what would you guys do? [Re: Kryptic]
    #13015305 - 08/07/10 12:08 AM (14 years, 5 months ago)

Quote:

I'd like to know where I went wrong.



The only other point of concern would be in your grain preparation method.  What provisions for grain preparation did you take?


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How I Get Stuff done. - My Reference Guide. - My Grows.

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Re: Some advice, what would you guys do? [Re: Kryptic]
    #13015314 - 08/07/10 12:10 AM (14 years, 5 months ago)

Another advice though, be patient and dont G2G 90-95% jars.
Wait till full colonization.
I'm not saying that it won't work, but your chances are 50/50.
Just wait out that extra day or two next time.

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Re: Some advice, what would you guys do? [Re: stonesun]
    #13015325 - 08/07/10 12:12 AM (14 years, 5 months ago)

It was either grain prep/not waiting full colonization/filtration (wet filter) issue.

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Re: Some advice, what would you guys do? [Re: hamloaf]
    #13015426 - 08/07/10 12:37 AM (14 years, 5 months ago)

Quote:

hamloaf said:
Quote:

I'd like to know where I went wrong.



The only other point of concern would be in your grain preparation method.  What provisions for grain preparation did you take?




I took 10 cups of rye, rinsed about 4 times till the water was clear, mixed in a tbspn of Gypsum, soaked for 24hrs.  Boiled for 10min, strained very well as they were steaming.  Before they went in the jars I tested them for dryness by putting a few tbspns on toilet tissue for 15 seconds I then removed the grains and the tissue was bone dry.  I watched  RR's "5 steps to perfect grain spawn" video.

Quote:

stonesun said:Another advice though, be patient and dont G2G 90-95% jars. Wait till full colonization.




Thanks for the advice.  Boy there is so much info on this site some of it conflicts and I know some of it is pretty dated so sometimes it's difficult to discern which info to follow.

I read somewhere, I thought it was RR that with grain jars you don't necessarily wait till 100% colonization but just slightly under when the myc is in it's peak growth phase.  Now I may have read all that completely wrong or just totally imagined it. lol

My filters didn't seem wet but I'm not certain.  I definitely may have shook too soon.  I tried to shake once at my best estimate of about 15% and again at what I thought was about 70%.

Interesting note, some jars did make it to full colonization.


--------------------
   

Dunking the grains: results and discussion | Grain to grain (G2G) in a SAB | Elementary Coir tek | Frank's proper pasteurization tek | Frank's simple coir\verm prep | RR notes | How to dial in your monotubs like a boss. | Pastywhyte's Easy Agar Tek | Frank's agar media journal

"Walk tall, kick ass, learn to speak Arabic, love music and never forget you come from a long line of truth-seekers, lovers and warriors" -Hunter S. Thompson

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Re: Some advice, what would you guys do? [Re: Kryptic]
    #13015487 - 08/07/10 12:50 AM (14 years, 5 months ago)

I searched it for ya'!

Quote:

RogerRabbit said:
I wouldn't and this is why:  Sterilzation only gives a 'window of opportunity' and is never complete.  If you g2g with uncolonized grains, those uncolonized kernels might sit for another two weeks in the receiving jar before the mycelium gets to them, thus they're outside their window, and more likely to contaminate. 

Let the jars fully colonize.  Being in a hurry never benefits mycology.
RR



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Re: Some advice, what would you guys do? [Re: stonesun]
    #13015572 - 08/07/10 01:17 AM (14 years, 5 months ago)

Thanks, told ya I probably just made that up and sorry to RR for attaching his name to my inability to read and follow suggestions.  :smile:  I can't believe I got that so wrong I read that doc I also now see where he recommends only 1 shake, damn, and I thought I was good at following direction, lol.  Well, I still have the colonized jars, I'll keep my fingers crossed on the G2Gs who knows, and I'll just keep trying, on the plus side I learn more from my fails then I do from my wins.  Thanks guys! :thumbup:


--------------------
   

Dunking the grains: results and discussion | Grain to grain (G2G) in a SAB | Elementary Coir tek | Frank's proper pasteurization tek | Frank's simple coir\verm prep | RR notes | How to dial in your monotubs like a boss. | Pastywhyte's Easy Agar Tek | Frank's agar media journal

"Walk tall, kick ass, learn to speak Arabic, love music and never forget you come from a long line of truth-seekers, lovers and warriors" -Hunter S. Thompson

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Re: Some advice, what would you guys do? [Re: stonesun]
    #13015750 - 08/07/10 02:49 AM (14 years, 5 months ago)

That is not true though. After sterilization, a medium is free of living organisms. That is the definition of microbiological sterilization. A properly sterilized jar will keep "forever" (in reality they tend to dry out after a few months unless closed off in some way).

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Re: Some advice, what would you guys do? [Re: Kryptic]
    #13016383 - 08/07/10 10:04 AM (14 years, 5 months ago)

So, I'm about to dump a bunch of jars, what an epic fail, days of work down the drain.  Some of these I would say were over 95% colonized.  Is there anything I can do to potentially get some shrooms from them so it's not a total wash? ie bury them in the garden and cross my fingers.


--------------------
   

Dunking the grains: results and discussion | Grain to grain (G2G) in a SAB | Elementary Coir tek | Frank's proper pasteurization tek | Frank's simple coir\verm prep | RR notes | How to dial in your monotubs like a boss. | Pastywhyte's Easy Agar Tek | Frank's agar media journal

"Walk tall, kick ass, learn to speak Arabic, love music and never forget you come from a long line of truth-seekers, lovers and warriors" -Hunter S. Thompson

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Re: Some advice, what would you guys do? [Re: Kryptic]
    #13016399 - 08/07/10 10:09 AM (14 years, 5 months ago)

Click on the link below for a solution.


Spent and Contaminated Substrates. Here's a Solution!


:robblerobble:


HL


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How I Get Stuff done. - My Reference Guide. - My Grows.

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Re: Some advice, what would you guys do? [Re: hamloaf]
    #13016894 - 08/07/10 12:07 PM (14 years, 5 months ago)

I just disposed of 7 jars in the way you described.  Some of the didn't smell too bad, all of them had a mushie smell, a couple of them had a really off sourness to them, I'm guessing some flavor of bacillus, I know it from brewing beer.  The mushie gods did not smile on me for this grow, hopefully I'll fare better next time.


--------------------
   

Dunking the grains: results and discussion | Grain to grain (G2G) in a SAB | Elementary Coir tek | Frank's proper pasteurization tek | Frank's simple coir\verm prep | RR notes | How to dial in your monotubs like a boss. | Pastywhyte's Easy Agar Tek | Frank's agar media journal

"Walk tall, kick ass, learn to speak Arabic, love music and never forget you come from a long line of truth-seekers, lovers and warriors" -Hunter S. Thompson

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Re: Some advice, what would you guys do? [Re: Kryptic]
    #13017015 - 08/07/10 12:38 PM (14 years, 5 months ago)

In your case, with the spawn, I would get a bag of cow manure (field aged manure would be best) or prepare some coir and place the manure or coir over the spawn so the spawn could colonize the manure or coir.  Place a sheet of clear plastic with slashes in it for GE over the substrate and check it every day for moisture content, watering when the substrate becomes dehydrated.  Once the substrate is fully colonized,  remove the plastic and cover with leaves or grass clippings watering in once in the morning everyday or every other day as needed.  Mother nature will take care of the rest.
HL


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

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