Home | Community | Message Board

Original Seeds Store
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Cultivation Supplies   PhytoExtractum Buy Bali Kratom Powder   Myyco.com Isolated Cubensis Liquid Culture For Sale   Bridgetown Botanicals CBD Concentrates   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: < Back | 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineDamion5050
Mush Doctor
Male User Gallery

Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation ! Flag
Last seen: 3 years, 11 months
Trusted Cultivator
Re: cloning question [Re: hamloaf]
    #13013614 - 08/06/10 05:21 PM (14 years, 5 months ago)

I mainly use LC's when doing like spawn bags or if I need to knock up a bunch of jars in a hurry..  Other wise I take my time and do agar to grain the g2g

Extras: Filter Print Post Top
OfflinehamloafM
Q-dood ®©™√
Male User Gallery

Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 day, 2 hours
Trusted Cultivator
OG Cultivator
Re: cloning question [Re: Numinosum]
    #13013632 - 08/06/10 05:25 PM (14 years, 5 months ago)

Quote:

Numinosum said:
Oh, btw HL, I just bought a 40lb of rice bran to try out. I will post the results in your thread.



:highfive:

Cool.  Do that.  You won't be disappointed that you replaced BRF for Rice Bran, I guarantee it!.  For cubes, I will never use BRF again.  How did you like that price?


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

Extras: Filter Print Post Top
InvisibleNuminosum
President of Turd Town
Male User Gallery


Registered: 05/19/09
Posts: 1,175
Re: cloning question [Re: hamloaf]
    #13013647 - 08/06/10 05:28 PM (14 years, 5 months ago)

Yeah 40lbs for $22. I don't usually use brf but after reading your thread I got motivated to go get some rice bran
and make some blocks.


--------------------
...within my memory is the knowledge of hyper-light drive ships and how to build them.



Doc_T's Efficiency Challenge

Extras: Filter Print Post Top
OfflinehamloafM
Q-dood ®©™√
Male User Gallery

Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 day, 2 hours
Trusted Cultivator
OG Cultivator
Re: cloning question [Re: Damion5050]
    #13013662 - 08/06/10 05:31 PM (14 years, 5 months ago)

Quote:

Damion5050 said:
I mainly use LC's when doing like spawn bags or if I need to knock up a bunch of jars in a hurry..  Other wise I take my time and do agar to grain the g2g



Me too, exactly except I don't get in a hurry.  LC's to spawn bags.  I knock my jars up with agar always, unless I am testing LC.  I am currently breaking that habit of LC and am going for the agar wedge to spawn bag and g2g with spawn bags in front of a flowhood.  I have had it with LC's, agar is soooo much better, cheaper, efficient, concise, and easier than LC.  Plus it makes you feel like a mad scientist.
HL


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

Extras: Filter Print Post Top
OfflineDamion5050
Mush Doctor
Male User Gallery

Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation ! Flag
Last seen: 3 years, 11 months
Trusted Cultivator
Re: cloning question [Re: hamloaf]
    #13013673 - 08/06/10 05:33 PM (14 years, 5 months ago)

LoL the only down side to agar in some cases is the amount of wedges u need to use to get a quart jar to colonize in a timely manner.

I sometimes use like a 1/2 pint jar the do grain LC with that to speed it up some.

Extras: Filter Print Post Top
InvisibleNuminosum
President of Turd Town
Male User Gallery


Registered: 05/19/09
Posts: 1,175
Re: cloning question [Re: Damion5050]
    #13013702 - 08/06/10 05:38 PM (14 years, 5 months ago)

I'll be making my first inoculations with agar tonight, I figure I'll take a tiny piece to a new
dish, then use the rest for my jars, bags. I have a different "substrain" to test on each.


--------------------
...within my memory is the knowledge of hyper-light drive ships and how to build them.



Doc_T's Efficiency Challenge

Extras: Filter Print Post Top
OfflinehamloafM
Q-dood ®©™√
Male User Gallery

Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 day, 2 hours
Trusted Cultivator
OG Cultivator
Re: cloning question [Re: Damion5050]
    #13013856 - 08/06/10 06:11 PM (14 years, 5 months ago)

Quote:

the only down side to agar in some cases is the amount of wedges u need to use to get a quart jar to colonize in a timely manner.




:what:  When using single section petri dishes or pint jars for agar to make MS wedges for fast colonization, I cut my agar into wedges like a pizza.  I get 8 wedges out of a jar or single section petri dish and never have any colonization issues as far as speed is concerned and I don't shake.  I think I can do the same with half the amount of an agar wedge too, getting 16 wedges out of a single section petri of or a jar.  It truly is amazing, how in this particular hobby, like no other, the things that work for one doesn't work for another.
HL


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

Extras: Filter Print Post Top
Invisiblewygram
Myconaut

Registered: 01/28/07
Posts: 573
Trusted Cultivator
Re: cloning question [Re: hamloaf]
    #13013890 - 08/06/10 06:16 PM (14 years, 5 months ago)

Quote:

hamloaf said:
[I get 8 wedges out of a jar or single section petri dish and never have any colonization issues as far as speed is concerned and I don't shake.



Those are some giant wedges. You only really need a tiny rice sized piece, then once the mycelium takes to the grain to about a quarter sized growth shake to redistribute and then shake once again at the normal 30%.


--------------------
Changing your mind is one of the best ways of finding out whether or not you still have one.

Extras: Filter Print Post Top
OfflinehamloafM
Q-dood ®©™√
Male User Gallery

Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 day, 2 hours
Trusted Cultivator
OG Cultivator
Re: cloning question [Re: wygram]
    #13014056 - 08/06/10 06:54 PM (14 years, 5 months ago)

Quote:

wygram said:
Those are some giant wedges. You only really need a tiny rice sized piece, then once the mycelium takes to the grain to about a quarter sized growth shake to redistribute and then shake once again at the normal 30%.




Yes wygram, they are.  I have become aware of that the agar wedges I was using were excessive, through trial and error.  I don't shake my jars at all, not once during colonization, as stated earlier, I am never in a rush (when it comes to my grows).  It barley happens but I just hate it when my jars don't recover from the shake.  It get's me fightin' mad.  I knead apart spawn bags however, at 25 percent colonization because spawn bags allow you to be more gentle with the myc come time to "shake".  I just drop a wedge onto the top of the grains and two weeks later, full colonization.  It was nice to talkin' to you, wygram. Have a good weekend and please, POAST MOAR!!  :crankey:


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery

Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 10 months
Trusted Cultivator
OG Cultivator
Re: cloning question [Re: OMackaRoni]
    #13014361 - 08/06/10 08:07 PM (14 years, 5 months ago)

Quote:

OMackaRoni said:
it's intimidating to me because of the use of agar and a petri dish. those things sound very high tek to my fairly noob mind.




Watch the agar/petri dish video, the cloning video, and the strain isolation video.  Bear in mind, these are only sample clips from the DVD, but they'll get the point across and it won't seem so hard.  Once you get those down, watch the master culture slant video to see how we store our cultures for long term use.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

Extras: Filter Print Post Top
InvisibleJavadog
Continuing along
Male User Gallery


Registered: 05/03/10
Posts: 7,385
Loc: USA Flag
Trusted Cultivator
Re: cloning question [Re: Damion5050]
    #13014462 - 08/06/10 08:31 PM (14 years, 5 months ago)

Quote:

Damion5050 said:
I think the same way I don't know why more people don't use agar...  I mean it really aint that hard.  After my second try or so with agar I had my technique down and knew what I was doing and now it is super super easy..




While I had planned to move toward agar, and have already made a batch
and poured my first dishes...I must admit that I am being forced to
advance the schedule as I am having trouble with infected LCs.

This is good in a way, as I will be working with prints,
and some I made myself, so agar was coming.


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org

Extras: Filter Print Post Top
Invisiblewygram
Myconaut

Registered: 01/28/07
Posts: 573
Trusted Cultivator
Re: cloning question [Re: Javadog]
    #13015309 - 08/07/10 12:09 AM (14 years, 5 months ago)

Quote:

Javadog said:
This is good in a way.



Yes it is. Agar is the superior spore starting method because you can ensure you have a clean culture before inoculating another medium. Since mushrooms are grown under non-sterile conditions, there is always a chance a print will contain some contaminant spores.

And it really is...
Quote:

Damion5050 said:
super super easy..





--------------------
Changing your mind is one of the best ways of finding out whether or not you still have one.

Extras: Filter Print Post Top
Offlineanathema
pǝsnɟuoƆ
Male


Registered: 03/13/05
Posts: 44
Loc: BC, Canada Flag
Last seen: 12 years, 3 months
Re: cloning question [Re: wygram]
    #13015404 - 08/07/10 12:31 AM (14 years, 5 months ago)

I know it is more of an agar thread as opposed to LC but I really am enjoying the mycellium water method.

agar (the poster) posted this tek:

http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6817701/an/0/page/0

You start with a healthy grain jar so are almost certain you have a clean starting point, since it is usually easy to tell if you have a contam'd grain jar.

I injected my grain jar with water, sucked it out into a few 20cc syringes, then later used the myc water, worked perfectly and much faster than multispore.

Being almost sure of a clean sample, plus fast colonization etc is great. No way to isolate as per agar of course, but thats life.


--------------------
--
Not all those who wander are lost.

Extras: Filter Print Post Top
InvisibleJavadog
Continuing along
Male User Gallery


Registered: 05/03/10
Posts: 7,385
Loc: USA Flag
Trusted Cultivator
Re: cloning question [Re: wygram]
    #13015439 - 08/07/10 12:39 AM (14 years, 5 months ago)

Quote:

wygram said:
Quote:

Javadog said:
This is good in a way.



Yes it is. Agar is the superior spore starting method because you can ensure you have a clean culture before inoculating another medium. Since mushrooms are grown under non-sterile conditions, there is always a chance a print will contain some contaminant spores.

And it really is...
Quote:

Damion5050 said:
super super easy..








The agar dishes did confirm that my syringes are contaminated
with bacteria.  I want to do the hot pour method rather than try
to take a clean sample.

Do you recall the temperature that this should be done at?

Actually I posted this in a separate thread, with photos,
so I will stop here.


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org

Extras: Filter Print Post Top
OfflinehamloafM
Q-dood ®©™√
Male User Gallery

Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 day, 2 hours
Trusted Cultivator
OG Cultivator
Re: cloning question [Re: Javadog]
    #13015969 - 08/07/10 06:08 AM (14 years, 5 months ago)

Quote:

The agar dishes did confirm that my syringes are contaminated
with bacteria.



What makes you so sure that the agar confirmed that your syringe(s) are contaminated?  How do you know it's not your sterile procedure?
Quote:

I want to do the hot pour method rather than try
to take a clean sample.  Do you recall the temperature that this should be done at?



Not specific temperature is applied.  Pour your agar once the container has cooled just enough to be handled with your hands but still quite hot.  Just typing this reply and thinking about pouring agar is making my hands burn.
HL


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

Extras: Filter Print Post Top
InvisibleJavadog
Continuing along
Male User Gallery


Registered: 05/03/10
Posts: 7,385
Loc: USA Flag
Trusted Cultivator
Re: cloning question [Re: hamloaf]
    #13017892 - 08/07/10 04:02 PM (14 years, 5 months ago)

Ham,

Well, what I have to go on is my limited experience.

I had been making one good LC after another, posting comments like
the common "Karo < SITR < Honey < LDM+Dex"....and then I started these
next four strains and every LC batch of the three syringes went bad.

As to technique I place the jars in front of my flow hood to cool and work
there once they are cooled.  I flame sterilized the needle before each jar.

So, while I may have to admit there are other possibilities,
when I drop some spore solution onto agar dishes and see all three of the
problem strains show bacterial contams, where the one strain that
made clean LC is not, then I am inclined to suspect the source of the
spore solution.

I will work to clean these up on agar.  I need to confirm the
temperature that the hot agar is poured over the infected plate.

I am making ten new dishes today, and will have something to transfer
to once they grow through to the surface.

I just cannot allow Cambodian, Tanseki, and Elephant Dung
slip away!!!

JD


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org

Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2  [ show all ]

Shop: North Spore Cultivation Supplies   PhytoExtractum Buy Bali Kratom Powder   Myyco.com Isolated Cubensis Liquid Culture For Sale   Bridgetown Botanicals CBD Concentrates   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* Simple Cloning Question.. LucidDayDream 1,425 3 05/17/02 10:06 AM
by mew
* clone question whitesox 960 5 02/21/03 08:27 AM
by z@z.com
* HoneyTek and Tissue Cloning questions... UnclePimpy 1,352 2 02/10/02 07:31 PM
by Zen Peddler
* Reasking a cloning question, HELP MOE !!! QuitDrinking 791 2 01/03/03 10:53 AM
by Zen Peddler
* cloning (shrooms not humans), need expert opinion. QuitDrinking 2,277 5 12/30/02 06:50 AM
by Curious_George
* Cloning question *DELETED* MeetMeInEleusis 665 1 03/22/02 01:25 PM
by puscle
* Re: non-entheogenic mushroom cloning questions Anonymous 935 2 01/20/00 06:05 PM
by Anonymous
* Re: CLONING QUESTION Anonymous 600 2 05/09/00 06:05 AM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,688 topic views. 18 members, 72 guests and 65 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.009 seconds on 13 queries.