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Mycelio
Stranger


Registered: 06/24/08
Posts: 1,636
Loc: Berlin
Last seen: 4 months, 23 days
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leaking brasiliensis
#12833184 - 07/01/10 06:05 AM (13 years, 7 months ago) |
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Hi,
right now my first culture of Agaricus brasiliensis starts fruiting indoor. Casing and primordia formation went well. Of course most of the pins stalled, but now something weird is happening. The stems are sweating out lots of water droplets, already forming puddles on the casing layer.
What the heck does this mean?
Temperature is around 30°C today (86F) and humidity is just high enough, so my oysters won't dry out, should be between 60 and 70%RH. I guess I'll increase FAE to make it easier to evaporate more water from the surface of the primordia.
Carsten

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curenado
73rd Man



Registered: 04/01/03
Posts: 2,603
Loc: North Central Arkansas
Last seen: 9 months, 25 days
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Re: leaking brasiliensis [Re: Mycelio]
#12833226 - 07/01/10 06:27 AM (13 years, 7 months ago) |
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They look healthy? Pretty cool! I am waiting on blazei outdoors right now!
-------------------- Yours in the Natural State! "The woods are lovely, dark and deep; but I have patches to keep, and jars to sterilize before I sleep...."
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Hacendado
Here,There...and in between



Registered: 01/10/10
Posts: 1,274
Loc: Austria
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Re: leaking brasiliensis [Re: curenado]
#12833305 - 07/01/10 07:13 AM (13 years, 7 months ago) |
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I'm waiting for my spores to germinate 11 days and still nothing. I wish I have leaking problems right now
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curenado
73rd Man



Registered: 04/01/03
Posts: 2,603
Loc: North Central Arkansas
Last seen: 9 months, 25 days
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Re: leaking brasiliensis [Re: Hacendado]
#12834328 - 07/01/10 12:28 PM (13 years, 7 months ago) |
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Mine took so long thought they had failed and ended up losing half to mold but they finally did - thought it was jusr me...
-------------------- Yours in the Natural State! "The woods are lovely, dark and deep; but I have patches to keep, and jars to sterilize before I sleep...."
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Hacendado
Here,There...and in between



Registered: 01/10/10
Posts: 1,274
Loc: Austria
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Re: leaking brasiliensis [Re: curenado]
#12834519 - 07/01/10 01:12 PM (13 years, 7 months ago) |
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Youre not the only one, my friend
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Mycelio
Stranger


Registered: 06/24/08
Posts: 1,636
Loc: Berlin
Last seen: 4 months, 23 days
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Re: leaking brasiliensis [Re: Hacendado]
#12834781 - 07/01/10 02:06 PM (13 years, 7 months ago) |
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I was starting with a colonized dish, first transfer after multispore on agar. For a few weeks, growth speed was terribly slow and I also lost most of my initially inoculated jars. Some went green, the rest just stalled and died, independent of substrate or FAE. I'm still wondering about the reason.
Bubiradigljive, be patient and don't compare with Lennybernardino, he used extremely fresh spores. Usually it takes much longer. It may take another week or two until you see something.
Regarding those two mushrooms, I showed above... they are still flooding their bag. At least the liquid has no bad smell, it's like freshly made popcorn. Found a hint in GGMM, where Stamets relates brown spots and embedded water droplets in the stems to immature compost. About three quarters of my substrate were uncomposted, having a lower nitrogen content, so lack of available nutrients is a possibility, causing the mycelium to pump more liquid into the mushrooms to get enough nutes up there. Though this is just speculation, hoping there are no bacteria or maggots at work.
Carsten
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Mycelio
Stranger


Registered: 06/24/08
Posts: 1,636
Loc: Berlin
Last seen: 4 months, 23 days
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Re: leaking brasiliensis [Re: Mycelio]
#12847208 - 07/04/10 11:32 AM (13 years, 7 months ago) |
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In the end they turned brown, soft and collapsed, while the primordia in the second bag started showing the same symptoms. I think it is some kind of bacterial blotch, perhaps caused by a Pseudomonas species, due to an microbial imbalance in the casing layer, excessive moisture and lack of fresh air. Brought them outside now and will remove the casing layer, to bury the substrate blocks in untreated soil.
Carsten

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Mycelio
Stranger


Registered: 06/24/08
Posts: 1,636
Loc: Berlin
Last seen: 4 months, 23 days
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Re: leaking brasiliensis [Re: Mycelio]
#13010808 - 08/06/10 04:33 AM (13 years, 6 months ago) |
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After removing those rotten mushrooms and the casing layer I placed the substrate in a plant pot on my balcony, surrounded by sandy soil, keeping the surface moist. For weeks, nothing happened until I noticed something was pushing up the soil at one end. Four nice mushrooms were working their way up from deep below. Within three days I could harvest my first ABMs. Got 165g on ca. 1500g substrate. I guess this is OK for the first time.
The other block did nothing yet, while all the later ones overheated. Never let your bags touch each other during hot summer! At least for this strain, 35°C seems to be the upper limit.
Taste was OK, somehow nutty and a bit sweet. On the first bite I didn't notice anything special, later there was this almond flavour, though not as strong as the smell when crumbling up colonized substrate.
Carsten

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stonesun
Sclerotia Aficionado




Registered: 05/22/09
Posts: 5,444
Loc: 64º08'00"N 21º56'00"W
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Re: leaking brasiliensis [Re: Mycelio]
#13011095 - 08/06/10 07:41 AM (13 years, 6 months ago) |
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Those look beautiful!
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wisp
Registered: 04/13/08
Posts: 5,304
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Re: leaking brasiliensis [Re: stonesun]
#13011247 - 08/06/10 08:31 AM (13 years, 6 months ago) |
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Wow, very nice. They look to be of a fair size.
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