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TM2443
Lizard King



Registered: 08/03/10
Posts: 394
Last seen: 14 years, 3 months
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Unlimited mycelium
#13008312 - 08/05/10 04:59 PM (14 years, 5 months ago) |
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Ok, what if I took 1 fully colonized jar of mycelium and blended it, (possibly with a little h202?) then used the slurry to inoculate 6 new jars and so on. Or skip the blender and just break off chunks of myc. and try to put them in the brf jars (in a sterile way)
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Unlimited mycelium [Re: TM2443]
#13008345 - 08/05/10 05:04 PM (14 years, 5 months ago) |
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Grain to grain... there's a video about it. 
But with pf cakes, no. That dry layer is contaminated. And how do you plan to sterilize the blender?
And please forget peroxide exists, it's useless.
-------------------- You make it all possible. Doesn't it feel good?
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Numinosum
President of Turd Town



Registered: 05/19/09
Posts: 1,175
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Re: Unlimited mycelium [Re: TM2443]
#13008356 - 08/05/10 05:06 PM (14 years, 5 months ago) |
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It would be insanely difficult to do this in a sterile manner.
But lucky for you, this is not a new hobby and the result you are looking for has been desired in the past. Enjoy!
Bonus: You can still use the donor jar!
-------------------- ...within my memory is the knowledge of hyper-light drive ships and how to build them.
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Diablojoe420
Nomad



Registered: 03/27/10
Posts: 230
Loc: South Carolina, US
Last seen: 14 years, 11 days
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Re: Unlimited mycelium [Re: TM2443]
#13008370 - 08/05/10 05:09 PM (14 years, 5 months ago) |
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I thought about doing this but you have to break through that dry vermiculite "filter" to really knock up the jar. Probably better used with WBS or Rye Seed--getting a bag of both this weekend.
No need to blend the mycelium or add anymore water because then you're throwing off the moisture content of your substrate (I think), just crumble up the cake and pour a little in each jar.
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Numinosum
President of Turd Town



Registered: 05/19/09
Posts: 1,175
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You don't NEED to have a verm filter. You could always make a filtered lid if you wanted to inoculate without a syringe, but don't want a pc.
-------------------- ...within my memory is the knowledge of hyper-light drive ships and how to build them.
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TM2443
Lizard King



Registered: 08/03/10
Posts: 394
Last seen: 14 years, 3 months
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Re: Unlimited mycelium [Re: Doc_T]
#13008402 - 08/05/10 05:17 PM (14 years, 5 months ago) |
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Quote:
Doc_T said:
But with pf cakes, no. That dry layer is contaminated.
Why does the dry verm layer matter? can't I just wipe it off like usual and wash the cake? Or even just cut that part of the cake off. And why is peroxide useless? I see a lot of people on here having success with it.
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Numinosum
President of Turd Town



Registered: 05/19/09
Posts: 1,175
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Re: Unlimited mycelium [Re: TM2443]
#13008431 - 08/05/10 05:23 PM (14 years, 5 months ago) |
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Why does the dry verm layer matter?
go into your basement, take the filter out of your furnace, give it a lick.
No fucking way right? The dry verm layer is a filter. It has filtered out all the nasties you don't want in your jar.
Can't I just wipe it off like usual and wash the cake?
Sure. If your going to fruit it. The cake is resistant to contamination now since there is no "vacancy" for new organisms to move in.
That doesn't mean they won't be landing all over it. So if you take some fully colonized sub thats covered in spores etc, and put it on in uncolonized sub, those contams will move right on in.
And why is peroxide useless? I see a lot of people on here having success with it.
Doing what? Getting rid of cobweb? Yup. Sterilization? Nope.
-------------------- ...within my memory is the knowledge of hyper-light drive ships and how to build them.
Doc_T's Efficiency Challenge
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Unlimited mycelium [Re: Numinosum]
#13008527 - 08/05/10 05:53 PM (14 years, 5 months ago) |
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Quote:
Numinosum said: You don't NEED to have a verm filter. You could always make a filtered lid if you wanted to inoculate without a syringe, but don't want a pc.
Here you go. Doing it this way, you can shake and then do cake 2 cake. Talk about explosive growth, I bet that really takes off.
Polyfil filters, cheap and easy.
-------------------- You make it all possible. Doesn't it feel good?
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TM2443
Lizard King



Registered: 08/03/10
Posts: 394
Last seen: 14 years, 3 months
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Re: Unlimited mycelium [Re: Numinosum]
#13008544 - 08/05/10 05:57 PM (14 years, 5 months ago) |
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Ok well if you can't spawn with brf cakes because they have too many contaminates due to the verm layer, how do casings work? Don't casings provide a "vacancy" for new organisms to move into?
And why would any other type of grain be better in terms of contaminating your new substrate? Is it because a diffeerent type of filter is used?
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Unlimited mycelium [Re: TM2443]
#13008595 - 08/05/10 06:07 PM (14 years, 5 months ago) |
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Kid, find a tek and follow it. Don't reinvent the wheel.
-------------------- You make it all possible. Doesn't it feel good?
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mutetongue
Learner

Registered: 04/16/10
Posts: 50
Last seen: 10 years, 9 months
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Re: Unlimited mycelium [Re: Doc_T]
#13008628 - 08/05/10 06:14 PM (14 years, 5 months ago) |
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Theres a Tek on here somewhere (wish I could remember who posted it) where they knock up a jar of WBS, then add sterilized water through an injection port, shake it up, then extract the mycelium water into an LC jar.
why dont you try that?
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Flowing
Monkey

Registered: 08/16/09
Posts: 323
Last seen: 7 years, 10 months
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-------------------- He believed that educated people
could make up their own minds. His motto, as head of one of the first and
most important review panels, was great encouragement: "We're not here
to play God."
-DMT: The Spirit Molecule
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Numinosum
President of Turd Town



Registered: 05/19/09
Posts: 1,175
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Re: Unlimited mycelium [Re: TM2443]
#13008676 - 08/05/10 06:24 PM (14 years, 5 months ago) |
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Quote:
TM2443 said: Ok well if you can't spawn with brf cakes because they have too many contaminates due to the verm layer, how do casings work? Don't casings provide a "vacancy" for new organisms to move into?
And why would any other type of grain be better in terms of contaminating your new substrate? Is it because a diffeerent type of filter is used?
If your questions are because your a stubborn dick than F-off.
If your questions are because you want to learn. So be it.
The layer has the contaminates not the cake. But when you dispose of the verm layer your shaking the nasties everywhere. Including all over the cake. Whenever you are adding something to a sterile, uncolonized substrate, you want your additive to be sterile.
As for your different types of grain question, its not the type of grain, its how its prepared. When you do a G2G transfer, you want the entire process to be sterile, so that no contamination can be introduced. Thats impossible when working with a verm layer.
As far as the casing layer goes..
I want to avoid using the term "non-nutritious". But for our purposes thats exactly what it is.
You wouldn't use manure as a casing. It would sure as shit contaminate.
When you are fruiting, spores etc are going to land all over the place. Problem (for them) is they need a place to live. If theres no real estate, the pose no issues.
Look up a term. I believe its microbiota antagonism.
-------------------- ...within my memory is the knowledge of hyper-light drive ships and how to build them.
Doc_T's Efficiency Challenge
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TM2443
Lizard King



Registered: 08/03/10
Posts: 394
Last seen: 14 years, 3 months
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Re: Unlimited mycelium [Re: Flowing]
#13008680 - 08/05/10 06:25 PM (14 years, 5 months ago) |
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Ok thanks everybody for all your help
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TM2443
Lizard King



Registered: 08/03/10
Posts: 394
Last seen: 14 years, 3 months
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Re: Unlimited mycelium [Re: Numinosum]
#13008703 - 08/05/10 06:29 PM (14 years, 5 months ago) |
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Quote:
Numinosum said:
If your questions are because your a stubborn dick than F-off.
If your questions are because you want to learn. So be it.
Yes I'm here to learn as much as possible. That's why I invest so much of my time into this hobby. And no I'm not being a dick... dick
Edited by TM2443 (08/05/10 06:31 PM)
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Numinosum
President of Turd Town



Registered: 05/19/09
Posts: 1,175
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Re: Unlimited mycelium [Re: TM2443]
#13008766 - 08/05/10 06:44 PM (14 years, 5 months ago) |
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Hey, I'm just trying to teach you what I know.
You last questions just seemed like "oh yeah? well.."
But I wasn't sure. Plus I started drinking a couple hours ago.. you know being thursday and all.
-------------------- ...within my memory is the knowledge of hyper-light drive ships and how to build them.
Doc_T's Efficiency Challenge
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shangrila
member



Registered: 02/18/04
Posts: 623
Last seen: 1 year, 11 months
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Re: Unlimited mycelium [Re: TM2443]
#13008924 - 08/05/10 07:24 PM (14 years, 5 months ago) |
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Quote:
TM2443 said: Ok well if you can't spawn with brf cakes because they have too many contaminates due to the verm layer, how do casings work? Don't casings provide a "vacancy" for new organisms to move into?
And why would any other type of grain be better in terms of contaminating your new substrate? Is it because a diffeerent type of filter is used?
just find something that works, and grow yourself some shrooms. in the meantime, crack open a beer and read up on succesfull grows and simple grain to bulk teks.
i tried different and new things for a while without being ready enough and ended losing 6-7 months of work, decided to go back to basics and follow teks to a t and i got me a garden! cheers.
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