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InvisibleDoc_T
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Re: I want to share my Agar Technique. [Re: Doc_T]
    #12986573 - 08/01/10 06:40 PM (14 years, 5 months ago)

So the day this tread happened, I needed to make some agar plates.
And I had empty PF jars. You can see where this is going...

My opinion: not bad at all.
Consider that most newbies have everything but agar flakes.
And the jars are heavy, but that works for some people. Easier to handle.

My own personal problem: my apartment is not level.
Jars allow a deep enough agar patty without spilling.

Not ideal, for a number of reasons RR mentioned.
But to clean up a free print or for My First Clone... go for it. :thumbup:
I made 10 more today, and left 5 petri dishes empty.


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Offline2ndChancesRDivine
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Re: I want to share my Agar Technique. [Re: Doc_T]
    #12986688 - 08/01/10 06:55 PM (14 years, 5 months ago)

I don't have the cash on hand to pay for agar supplies either. Im cleaning three strains right now, 2 PE clones and EQ spores as well. Its working pretty good. I love that PDA is so easy to make. I wonder if you can sub turbinado aka sugar in the raw for dextrose.


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InvisibleDoc_T
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Re: I want to share my Agar Technique. [Re: 2ndChancesRDivine]
    #12986704 - 08/01/10 06:58 PM (14 years, 5 months ago)

Should be fine. You use potato flakes?


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Offline2ndChancesRDivine
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Re: I want to share my Agar Technique. [Re: Doc_T]
    #12986729 - 08/01/10 07:01 PM (14 years, 5 months ago)

Nah, I like to murder spuds as I have a ton of them. Its a bitch doing smaller quantities. 250ml will evaporate quickly in 10 mins if you accidentally leave the stove on high :wink:


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Re: I want to share my Agar Technique. [Re: 2ndChancesRDivine]
    #12986978 - 08/01/10 07:48 PM (14 years, 5 months ago)

SITR works ive used it in agar


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Re: I want to share my Agar Technique. [Re: truskool]
    #12988593 - 08/02/10 12:46 AM (14 years, 5 months ago)

Better or worse than dextrose?


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Re: I want to share my Agar Technique. [Re: 2ndChancesRDivine]
    #12988597 - 08/02/10 12:48 AM (14 years, 5 months ago)

Ive never used dex for my agar:shrug:


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Invisibleshymanta
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Re: I want to share my Agar Technique. [Re: truskool]
    #12990542 - 08/02/10 12:59 PM (14 years, 5 months ago)

You can get dextrose, agar agar, malt extract, and perhaps nutritional yeast as well if you like to use it.  If you buy from the right sellers, you can get all you need for making agar to pour, including shipping, for about the cost of your average "munchies trip" to the grocery store.

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InvisibleDoc_T
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Re: I want to share my Agar Technique. [Re: shymanta]
    #12990559 - 08/02/10 01:02 PM (14 years, 5 months ago)

All of those are available at local health stores, no need to even order them.
Go to health store, get agar supplies and get munchies too. :yesnod: Scope out the granola chicks.


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Invisibletruskool
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Re: I want to share my Agar Technique. [Re: Doc_T]
    #12990581 - 08/02/10 01:05 PM (14 years, 5 months ago)

Quote:

Doc_T said:
:yesnod: Scope out the granola chicks.



IDK doc granola chicks and boxy.  Im begining to wonder about your choice in girls:rofl2:


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Re: I want to share my Agar Technique. [Re: rave420]
    #12990717 - 08/02/10 01:31 PM (14 years, 5 months ago)

Quote:

FooMan said:











I really like this design!!!  Specifically, the two holes at either side of the lid!  It seems to me that gas would more efficiently circulate according to convection currents, but still maintain low CO2 levels.  Rather than one hole in the middle, which seems as though the air could stagnate.  Just enough fresh air to discourage contaminants, but not enough to retard the mycelium's vegetation.

I have used Tyvek filters with only one hole with success on grain but I think will try two holes, and perhaps success rates will rise.  I've used this type of filter on agar filled jars.  It works.  Contaminated less than jars without filters.  Although, I have moved on to a clean room and flowhood work area, and petri dishes are much easier to handle (especially disposable ones), I still prefer glass dishes.






Your success rates will rise even from something as simple as doing work under a make-shift canopy made from four cans and a cookie sheet.  Reducing the amount of spores and dust that will rain down from the air in the room.  Same principle as a glovebox only not so isolated.  Clean the area and work with slow but efficient movements to avoid stirring the adjacent air.
:wink:

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Invisibleshymanta
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Re: I want to share my Agar Technique. [Re: Doc_T]
    #12990766 - 08/02/10 01:39 PM (14 years, 5 months ago)

Quote:

Doc_T said:
All of those are available at local health stores, no need to even order them.
Go to health store, get agar supplies and get munchies too. :yesnod: Scope out the granola chicks.





I've got to drive a ways to see granola chicks.  But Amazon is a click away, there's probably one on this page.  When I'm close to a health food store I like to get it there.  What granola chicks lack in conventional beauty, they more than make up for with their own individual beauties. Not to mention a healthy body is a lot more fun to play with. :grin:

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Re: I want to share my Agar Technique. [Re: shymanta]
    #12990974 - 08/02/10 02:13 PM (14 years, 5 months ago)

great post Doc_T

i knew there was a simpler way of doing this.

Check this out: next time use Guar Gum powder or Xanthan gum powder, enough to make it thick and you mix in some sugar / malt extract. It works just as well as the other stuff, except it is a bit more "gloopy". Instead of cutting a piece of agar, you can scoop a piece of gum instead.

As long as your method of keeping contaminants in check is okay you will do fine with any culture media in any container using any technique.

if anybody wants me to i will post a TEK for making great self-healing lids using felt as the gas exchange port. It involves two holes, one has a square of felt with micropore tape silicone'd to it and the other is a self healing injection port made from silicone.  Here's a pic, i'd be happy to do a writeup on how to make them, materials needed etc.


Edited by rave420 (08/02/10 02:21 PM)

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Offlineidunno
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Re: I want to share my Agar Technique. [Re: rave420]
    #12991022 - 08/02/10 02:22 PM (14 years, 5 months ago)

Doc-t has good choice of chicks,look at the avatar, unless thats him in drag-fooled me


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Invisibletruskool
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Re: I want to share my Agar Technique. [Re: idunno]
    #12991099 - 08/02/10 02:33 PM (14 years, 5 months ago)

thats boxxy the most annoying girl ever search youtube


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Re: I want to share my Agar Technique. [Re: truskool]
    #12991167 - 08/02/10 02:43 PM (14 years, 5 months ago)



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Offlinerave420
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Re: I want to share my Agar Technique. [Re: Doc_T]
    #12991190 - 08/02/10 02:47 PM (14 years, 5 months ago)

i associate boxxy lovers with 4chan and 4chan i associate with a lovely pile of poop. However, i used to be a member of their "community" some time ago, i eventually grew out of it and turned towards bigger, more important things.

I also recognize that i can't jump to conclusions, this is the shroomery after all, and while i'd like to see bwxsy naked i think the whole cult is going a bit too far.


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Invisibletruskool
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Re: I want to share my Agar Technique. [Re: Doc_T]
    #12991202 - 08/02/10 02:49 PM (14 years, 5 months ago)

Uhm she is cute in a pedofilish sort of way.
Case in point that vid just shows how fucking annoying she is and she just goes on and on with that voice.  Like fingernails on a chalk board IMHO


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InvisibleDoc_T
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Re: I want to share my Agar Technique. [Re: truskool]
    #12991221 - 08/02/10 02:53 PM (14 years, 5 months ago)

I sort of picked her by accident. Now everybody is used to it.
I'm not a channer, really not a pedo, and seriously, I can't fap to that. :crankey:

But she makes a fine avatar.


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Invisibletruskool
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Re: I want to share my Agar Technique. [Re: Doc_T]
    #12991232 - 08/02/10 02:54 PM (14 years, 5 months ago)

Quote:

Doc_T said:
I sort of picked her by accident. Now everybody is used to it.
I'm not a channer, really not a pedo, and seriously, I can't fap to that. :crankey:

But she makes a fine avatar.




For sure.


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Ask  AMU    for    the  best    mycology    advice  out  there

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Shop: OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Substrate Bags   Original Sensible Seeds High THC Strains   Myyco.com Isolated Cubensis Liquid Culture For Sale   Bridgetown Botanicals CBD Concentrates   Kraken Kratom Kratom Capsules for Sale   Unfolding Nature Unfolding Nature: Being in the Implicate Order


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