Home | Community | Message Board


Phytoextractum
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: ½ Pint Jars, Agar

Jump to first unread post. Pages: 1
Anonymous

h202 in honeywater
    #1297362 - 02/10/03 05:32 PM (14 years, 9 months ago)

i have some cyan mycelium on some alder, and want to make a syringe. i chose the honey tek cause i have honey, water, jars and peroxide, and no agar or money for it.

in the agar tek, you can add h2o2 before mycelium to help grow clean mycelium in the agar, but in the honeywater tek, you add the h2o2 AFTER the mycelium colonizes the jars. thus, in the honey tek, you must start off with clean mycelium. im not sure my myceliums cleanliness

is it possible to add h2o2 to honeywater before mycelium, to help with a clean colonization? (of course i will let the honeywater cool before adding anything)


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleJoshua
Holoman
Male

Registered: 10/27/98
Posts: 5,389
Loc: The Matrix
Re: h202 in honeywater [Re: Anonymous]
    #1297576 - 02/10/03 07:01 PM (14 years, 9 months ago)

If you are starting from a culture you should have no problem adding H2O2 before adding your culture to the solution.

I actually think it would be comparatively ineffective to add H2O2 after your culture has grown throughout the solution. If contams are present in the culture they are likely to survive because they produce their own peroxidases.

The only advantage I see to adding peroxide post culture is to prevent new contams from colonizing any undigested honey solution once the solution has been used to inoculate.

Joshua


--------------------
The Shroomery Bookstore

Great books for inquiring minds!

"Life After Death is Saprophytic!"


Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: h202 in honeywater [Re: Joshua]
    #1298490 - 02/11/03 04:54 AM (14 years, 9 months ago)

thanks, thats the answer i was looking for.

i didnt understand why we are to add h2o2 after cultures colonize either. it just says to do so in the honey tek


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: ½ Pint Jars, Agar

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* H. Peroxide in spore water NITRIC 2,399 16 10/08/14 03:19 PM
by weaksause
* Questions about MEA and peroxide... upupup 1,019 4 11/22/02 09:46 PM
by Joshua
* Agar Culture transfer to Liquid Culture Tek CompilerV 6,899 11 06/27/03 07:11 PM
by Compiler
* h202 discovery (NEW HIGH RES PICS UPDATED) (NEW UPDATE 17-6-08)
( 1 2 3 4 5 6 7 8 9 all )
Juke Adro
19,736 174 01/11/13 07:18 PM
by Juke Adro
* Honeywater/Birdseed petepetosa 1,039 7 06/13/01 05:40 AM
by MNmyc
* peroxide help - by mycofile Raadt 1,530 2 12/13/02 08:53 AM
by Azmodeus
* What is an effective use of Hydrogen Peroxide?
( 1 2 all )
garret 12,343 38 05/26/08 04:36 AM
by Tequila
* Re: Liquid mycelium with H202 Anonymous 1,122 6 02/11/00 08:31 AM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, bodhisatta
520 topic views. 1 members, 1 guests and 1 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Edabea
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.016 seconds spending 0.003 seconds on 16 queries.