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OfflineSMG513
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Contaminated prints?
    #12969997 - 07/29/10 12:19 AM (14 years, 5 months ago)

Ok... so i have had my success and many many contaminations... mostly from not having a place to do everything.

Anyway my question is about the spore prints i made from outdoor grows.
I did not take precautions to keep them as sterile as possible and was wondering if their is anyway to sterilize it for use as a syringe..?

End goal is a LC hopefully.. any help would be great! thanks!

Also i'm thinking of building a glove box for the process to help be more sterile.

Edited by SMG513 (07/29/10 12:20 AM)

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InvisibleEvilMushroom666
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Re: Contaminated prints? [Re: SMG513]
    #12970006 - 07/29/10 12:22 AM (14 years, 5 months ago)

One word. Agar.

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Offlinemushking
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Re: Contaminated prints? [Re: SMG513]
    #12970007 - 07/29/10 12:22 AM (14 years, 5 months ago)

LC is your best beat by far

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InvisibleJavadog
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Re: Contaminated prints? [Re: SMG513]
    #12970011 - 07/29/10 12:25 AM (14 years, 5 months ago)

Hello,

I am still making prints, and have not had to use one yet,
but being able to do this is one reason I am starting to use
agar, and why I built a laminar flow hood.

I think that pouring dishes would be pretty hard in a glove box.
(but possible I am sure)

I go into all of this because on a petri dish you can spot the
good growth and transfer it to a clean dish, and repeat until you
have something safe to grow with.

Other than that all I could think of would be to take all of the
cleanliness precautions and give it a shot. 

Look up sterile syringe teks in the search field above.

There are good ones.  I have managed to reuse my spore syringes
following these teks.

Good Luck!

JD


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org

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Offlinenewbie_shroomer
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Re: Contaminated prints? [Re: Javadog]
    #12970073 - 07/29/10 12:53 AM (14 years, 5 months ago)

Well Agar will show up any contams unlike LC which will look like myc growth as the mold has out sporulated yet.

So Agar is best but you will have to make sure you get everything right (sterile inc area etc..)

But a very simple way to keep your strain is grain LC! You can look for contams before making the LC and the process minimizes the possibility of introduction of contams.

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OfflinehamloafM
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Re: Contaminated prints? [Re: SMG513]
    #12970075 - 07/29/10 12:53 AM (14 years, 5 months ago)

Quote:

SMG513 said:
Ok... so i have had my success and many many contaminations... mostly from not having a place to do everything.

Anyway my question is about the spore prints i made from outdoor grows.
I did not take precautions to keep them as sterile as possible and was wondering if their is anyway to sterilize it for use as a syringe..?

End goal is a LC hopefully.. any help would be great! thanks!

Also i'm thinking of building a glove box for the process to help be more sterile.



Most effective way to clean wild prints from contaminates is to inoculate agar with your wild print's spores.  You can then transfer away clean mycelium from contaminates and inoculate more petri's until you have a clean culture.  Transfer clean mycelium away from contaminates inside a glove-box or clear plastic trash bag.  I use the trash bag because after one use you just toss it out, no cleaning.  Do not clean mycelium away from contaminates in front of a flowhood.  A flowhood will blow the contaminate mold spores through out your lab and home.  If you must do LC's, there is no better way to inoculate an LC than with a live culture agar wedge.
HL


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

Edited by LiquidMyce (07/29/10 03:53 AM)

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OfflineSMG513
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Re: Contaminated prints? [Re: hamloaf]
    #12971596 - 07/29/10 12:20 PM (14 years, 5 months ago)

k thanks for all the replies.. i will see what i can do by making an LC

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InvisibleDoc_T
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Re: Contaminated prints? [Re: SMG513]
    #12971610 - 07/29/10 12:22 PM (14 years, 5 months ago)

Quote:

SMG513 said:
k thanks for all the replies.. i will see what i can do by making an LC




No! Agar is the right tool for this task.

LC from wild print = fail. :thumbdown:


--------------------
You make it all possible. Doesn't it feel good?

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Offline2ndChancesRDivine
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Re: Contaminated prints? [Re: Doc_T]
    #12971794 - 07/29/10 12:58 PM (14 years, 5 months ago)

Only one guy on the thread said to do an LC and everyone else said agar :huxleyfacepalm:


--------------------
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All your RC are belong to me.

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OfflineSMG513
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Re: Contaminated prints? [Re: 2ndChancesRDivine]
    #13026381 - 08/09/10 12:31 PM (14 years, 5 months ago)

well i dont know where to get agar..

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InvisibleDoc_T
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Re: Contaminated prints? [Re: SMG513]
    #13026412 - 08/09/10 12:41 PM (14 years, 5 months ago)

:google:

Also, UTSE.

Also, asian markets or health food stores. :thumbup:

Also, it's pronounced like 'auger'. Ask for "auger auger".


--------------------
You make it all possible. Doesn't it feel good?

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InvisibleNuminosum
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Re: Contaminated prints? [Re: SMG513]
    #13026417 - 08/09/10 12:42 PM (14 years, 5 months ago)

Asian markets. Stores with science/lab supplies. Online.

Shit even the little shop that sells educational toys for children has it.


--------------------
...within my memory is the knowledge of hyper-light drive ships and how to build them.



Doc_T's Efficiency Challenge

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