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Amazon Shop: Agar, Malt Extract, Petri Dish

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Offlinecoming_up_roses
newbie

Registered: 04/12/02
Posts: 31
Last seen: 13 years, 5 months
need help with agar
    #1295726 - 02/10/03 08:19 AM (14 years, 8 months ago)

could someone please tell me how long it should take to see signs of mycelium growth in a petri dish with malt extract agar which was inoculated with ecuadorian spores and a spore syringe

thanks


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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: need help with agar [Re: coming_up_roses]
    #1295751 - 02/10/03 08:42 AM (14 years, 8 months ago)

Did you squirt water from syringe to inoc the agar ?


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


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Offlinecoming_up_roses
newbie

Registered: 04/12/02
Posts: 31
Last seen: 13 years, 5 months
Re: need help with agar [Re: tripndicular]
    #1295768 - 02/10/03 08:49 AM (14 years, 8 months ago)

i used a syringe on 2 plates and and just droped spores on 2 others there not contaminated because theres nothing growing


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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: need help with agar [Re: coming_up_roses]
    #1295807 - 02/10/03 09:01 AM (14 years, 8 months ago)

Okay first off .......
Squirting spore fluid is next to worthless , you have no way of controling "how many" spores are getting on agar . Have tried this self and found out .......sucks !
If was a really dark stringe ..........give it a few more days , who knows might get lucky .
Second .......
In most cases ... if spores are nice and fresh (viabile) , and environmemtal conditions are primo (at their best 84-86*F) , agar is well mixed ... you should see signs of growth(looks kinda hazy) with in 7 days from inoc date . But can take up to 14 days . IMHO , is best to use an inoculation loop instead of that airborne drop on method . Again this is MHO .


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


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Offlinecoming_up_roses
newbie

Registered: 04/12/02
Posts: 31
Last seen: 13 years, 5 months
Re: need help with agar [Re: tripndicular]
    #1295819 - 02/10/03 09:06 AM (14 years, 8 months ago)

thanks i'l leave it a bit longer


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Offlinefugu
Pooh-Bah

Registered: 01/06/03
Posts: 2,223
Loc: istanbul
Last seen: 7 years, 3 months
Re: need help with agar [Re: coming_up_roses]
    #1295852 - 02/10/03 09:16 AM (14 years, 8 months ago)

i took signs in 3 days trip(avarage from spors).and i thought that lots of others are same.i took sign in 20 hour later from my last incubation.maybe your mixing is a little bit hard
:wink: 


--------------------
mushroom culture history making ...Mr. Allan is the best .....


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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: need help with agar [Re: fugu]
    #1295895 - 02/10/03 09:35 AM (14 years, 8 months ago)

With real fresh , viable spores this is possible , have had happen once in 3 days . Many variables to factor in .......
But the average is more like 7-10 days for "positive" signs of mycelial growth .  :smirk: 


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinefugu
Pooh-Bah

Registered: 01/06/03
Posts: 2,223
Loc: istanbul
Last seen: 7 years, 3 months
Re: need help with agar [Re: tripndicular]
    #1295913 - 02/10/03 09:42 AM (14 years, 8 months ago)

>Many variables
call you tell me a little bit what are those.i did n t take the book yet.


--------------------
mushroom culture history making ...Mr. Allan is the best .....


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Amazon Shop: Agar, Malt Extract, Petri Dish

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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