Home | Community | Message Board


Magic-Mushrooms-Shop.com
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: Agar, Malt Extract, Petri Dish

Jump to first unread post. Pages: 1
Offlinecoming_up_roses
newbie

Registered: 04/12/02
Posts: 31
Last seen: 13 years, 11 months
need help with agar
    #1295726 - 02/10/03 08:19 AM (15 years, 2 months ago)

could someone please tell me how long it should take to see signs of mycelium growth in a petri dish with malt extract agar which was inoculated with ecuadorian spores and a spore syringe

thanks


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: need help with agar [Re: coming_up_roses]
    #1295751 - 02/10/03 08:42 AM (15 years, 2 months ago)

Did you squirt water from syringe to inoc the agar ?


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinecoming_up_roses
newbie

Registered: 04/12/02
Posts: 31
Last seen: 13 years, 11 months
Re: need help with agar [Re: tripndicular]
    #1295768 - 02/10/03 08:49 AM (15 years, 2 months ago)

i used a syringe on 2 plates and and just droped spores on 2 others there not contaminated because theres nothing growing


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: need help with agar [Re: coming_up_roses]
    #1295807 - 02/10/03 09:01 AM (15 years, 2 months ago)

Okay first off .......
Squirting spore fluid is next to worthless , you have no way of controling "how many" spores are getting on agar . Have tried this self and found out .......sucks !
If was a really dark stringe ..........give it a few more days , who knows might get lucky .
Second .......
In most cases ... if spores are nice and fresh (viabile) , and environmemtal conditions are primo (at their best 84-86*F) , agar is well mixed ... you should see signs of growth(looks kinda hazy) with in 7 days from inoc date . But can take up to 14 days . IMHO , is best to use an inoculation loop instead of that airborne drop on method . Again this is MHO .


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinecoming_up_roses
newbie

Registered: 04/12/02
Posts: 31
Last seen: 13 years, 11 months
Re: need help with agar [Re: tripndicular]
    #1295819 - 02/10/03 09:06 AM (15 years, 2 months ago)

thanks i'l leave it a bit longer


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinefugu
Pooh-Bah

Registered: 01/06/03
Posts: 2,223
Loc: istanbul
Last seen: 7 years, 9 months
Re: need help with agar [Re: coming_up_roses]
    #1295852 - 02/10/03 09:16 AM (15 years, 2 months ago)

i took signs in 3 days trip(avarage from spors).and i thought that lots of others are same.i took sign in 20 hour later from my last incubation.maybe your mixing is a little bit hard
:wink: 


--------------------
mushroom culture history making ...Mr. Allan is the best .....


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: need help with agar [Re: fugu]
    #1295895 - 02/10/03 09:35 AM (15 years, 2 months ago)

With real fresh , viable spores this is possible , have had happen once in 3 days . Many variables to factor in .......
But the average is more like 7-10 days for "positive" signs of mycelial growth .  :smirk: 


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinefugu
Pooh-Bah

Registered: 01/06/03
Posts: 2,223
Loc: istanbul
Last seen: 7 years, 9 months
Re: need help with agar [Re: tripndicular]
    #1295913 - 02/10/03 09:42 AM (15 years, 2 months ago)

>Many variables
call you tell me a little bit what are those.i did n t take the book yet.


--------------------
mushroom culture history making ...Mr. Allan is the best .....


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: Agar, Malt Extract, Petri Dish

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Benefits of Agar? celsius 2,768 7 07/18/01 11:28 AM
by Anno
* might be an advance agar question
( 1 2 all )
fugu 5,293 33 02/05/03 08:45 AM
by fugu
* best liquid/agar medium? gray1 1,036 1 05/16/01 01:01 PM
by egghead
* Using Agar medium directly from spore print?? djveiox 2,677 14 12/08/01 02:47 PM
by blahblahblah
* Anyone know how to do Agar well? malibu76 797 3 08/17/04 01:53 AM
by Anno
* Potato Dextrose Agar X Malt Extract Agar Alegalize 2,052 8 02/25/05 01:03 PM
by agar
* right malt extract? scrname 1,046 11 04/12/05 09:35 AM
by ohmatic
* cheapest glass petri dishes, agar, dextrose, etc Fluxburn 2,554 8 02/14/05 09:57 AM
by Roadkill

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
515 topic views. 17 members, 70 guests and 21 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
The HUP - Testing Kits
Please support our sponsors.

Copyright 1997-2018 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.006 seconds on 19 queries.