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Amazon Shop for: ½ Pint Jars, Agar, Brown Rice Flour, Calcium Carbonate, Malt Extract, Potato Dextrose, pH Test Strips

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Offlinefugu
Pooh-Bah

Registered: 01/06/03
Posts: 2,223
Loc: istanbul
Last seen: 6 years, 8 months
an idiot asking
    #1292476 - 02/09/03 06:16 AM (14 years, 1 month ago)

i will plan to use my half developed agars.i don t think a problem will occur.but i want to ask it ? sda s are very slow.


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mushroom culture history making ...Mr. Allan is the best .....


Edited by fugu (02/09/03 06:17 AM)


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OfflineCurious_George
You want abaloon?

Registered: 07/15/02
Posts: 1,065
Loc: La La Land
Last seen: 12 years, 14 days
Re: an idiot asking [Re: fugu]
    #1292486 - 02/09/03 06:19 AM (14 years, 1 month ago)

If you mean that your jars are only half done/ half white...

WAIT!!!!

Do not case them yet.. you could, but it's better to wait!!!

cg


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************************************.>>>>>
Here Johny,, have a joint.. all your friends are doing it!!


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InvisibleStainItBlue

Registered: 09/25/02
Posts: 261
Re: an idiot asking [Re: Curious_George]
    #1292493 - 02/09/03 06:22 AM (14 years, 1 month ago)

george hes talkin about agar plates...i think they may be only half covered? but im not totally sure what hes askin?


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Invisibleno-tone
Enema Bag Jones

Registered: 10/17/00
Posts: 1,091
Loc: Warm, Moist and Dark
Re: an idiot asking [Re: fugu]
    #1292497 - 02/09/03 06:24 AM (14 years, 1 month ago)

What are you planning to do with the agars? You may be able to just cut out the uncolonized portions.


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Man thinks. God laughs. - Jewish Proverb


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Offlinefugu
Pooh-Bah

Registered: 01/06/03
Posts: 2,223
Loc: istanbul
Last seen: 6 years, 8 months
Re: an idiot asking [Re: no-tone]
    #1292504 - 02/09/03 06:27 AM (14 years, 1 month ago)

incubate some grains and also why do i have cut out the un colonized parts..i put there some colonized parts and they will continue to grow


--------------------
mushroom culture history making ...Mr. Allan is the best .....


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InvisibleG a n j a
Pictish and proud
 User Gallery

Registered: 12/03/02
Posts: 7,860
Loc: Zone ate
Re: an idiot asking [Re: fugu]
    #1292507 - 02/09/03 06:29 AM (14 years, 1 month ago)

Be patient and wait :smile: they should fully colanise in a few days and then you have more mycelium to play with :smile:


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er


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OfflineCurious_George
You want abaloon?

Registered: 07/15/02
Posts: 1,065
Loc: La La Land
Last seen: 12 years, 14 days
Re: an idiot asking [Re: fugu]
    #1292513 - 02/09/03 06:30 AM (14 years, 1 month ago)

Fugu.. are you asking about agar plates , or grain jars???

cg


--------------------
************************************.>>>>>
Here Johny,, have a joint.. all your friends are doing it!!


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Invisibleno-tone
Enema Bag Jones

Registered: 10/17/00
Posts: 1,091
Loc: Warm, Moist and Dark
Re: an idiot asking [Re: fugu]
    #1292520 - 02/09/03 06:32 AM (14 years, 1 month ago)

If you are innoculating grain, you could probably just cut out some of the colonies and leave some to finish colonizing the plate.


--------------------
Man thinks. God laughs. - Jewish Proverb


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OfflineCurious_George
You want abaloon?

Registered: 07/15/02
Posts: 1,065
Loc: La La Land
Last seen: 12 years, 14 days
Re: an idiot asking [Re: no-tone]
    #1292529 - 02/09/03 06:35 AM (14 years, 1 month ago)

Oh, is that what he meant???

Yes, you can take a pice of growth from you agar and transfer it to grain!! be VERY clean tho!

cg


--------------------
************************************.>>>>>
Here Johny,, have a joint.. all your friends are doing it!!


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Offlinefugu
Pooh-Bah

Registered: 01/06/03
Posts: 2,223
Loc: istanbul
Last seen: 6 years, 8 months
Re: an idiot asking [Re: no-tone]
    #1292538 - 02/09/03 06:42 AM (14 years, 1 month ago)

thanks all. i have got some grains before. 2 days ago i soaked them and i made a plan to inoculate them from grains. but i do nt like its speed s/ grow fast and it seems maybe it will going to contaminate.i don t want risk my grains..i don t know yet but i have got some grains and just plates. they are in pc now. i will inoculate them from agars. and leave a little for some grow .i also got lots of plates.no matter.


--------------------
mushroom culture history making ...Mr. Allan is the best .....


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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: an idiot asking [Re: fugu]
    #1292554 - 02/09/03 06:49 AM (14 years, 1 month ago)

Half developed agars ??????????????????
Fugu ............. slow down a bit here .
What exactlly are you trying to ask ?
Also what is sda s ?


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


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Offlinefugu
Pooh-Bah

Registered: 01/06/03
Posts: 2,223
Loc: istanbul
Last seen: 6 years, 8 months
Re: an idiot asking [Re: tripndicular]
    #1292578 - 02/09/03 07:04 AM (14 years, 1 month ago)

trip make a search on agar types.but i like to give it directly.for these times
half developed :i transfered a part from another agar.and only half of the plate colonized yet.and as i said you before you didn y understand what you read. :wink:
here are agars sda is a type sabouraud.its ph is  a bit low .that is why pda and malt s are fast
take a look  on them
Agar Formulas & Info
Amaranth Soy Agar
20 grams amaranth flour
20 grams soy flour
9.5 grams agar
500 mL distilled water
Final pH: 6.0  Barley Malt Extract Agar
40 grams barley flour
2 grams malt extract
1 - 2 grams yeast extract (optional)
9.5 grams agar
500 mL distilled water
Final pH: 6.0  EntheoGenesis No. 442
10 grams amaranth flour
10 grams brown rice flour
10 grams potato flour
10 grams soy flour
2 grams malted barley
9.5 grams agar
500 mL distilled water 
Cornmeal Dextrose Agar
25 grams yellow cornmeal
2.5 grams dextrose
9.5 grams agar
500 mL distilled water
Final pH: 6.0  Dr. Pollock's Modified Agar
10 grams dried dog food
10 grams amaranth flour
2 grams dextrose or malt extract
9.5 grams agar
500 mL distilled water
Final pH is 6.2.  Malt Extract Agar
20 grams malt extract
100 mg potassium phosphate
    dibasic (K2HPO4)
100 mg calcium carbonate
9.5 grams agar
1 L distilled water 
Oatmeal Neopeptone Agar
40 grams oatmeal or oat flour
2 grams neopeptone (optional)
9.5 grams agar
500 mL distilled water
Final pH: 6.0  Modified Sabouraud's Medium
25 grams barley flour
5 grams dextrose
2 grams neopeptone (optional)
1 gram yeast extract
9.5 grams agar
500 mL distilled water
Final pH: 5.8  Potato Dextrose Yeast Agar
250 grams of washed, unpeeled potatoes
10 grams dextrose
1.5 grams yeast
15 grams agar
Slice potatoes 1/8 inch thick
Rinse slices clean in cool tap water
Final rinse with distilled water
Cook in distilled water until tender
Strain and set aside cooking water
Rinse potatoes with distilled water
Retain rinse water, discard potatoes
Add distilled water to make 1 litre
Bring liquid to slow boil
Add dry ingredients

Cooking & Usage Instructions 
The flask or bottle in which the medium is sterilized should never be more than 2/3 full in order to avoid boilover.
Plug the flask or bottle with non-absorbent cotton and cover with aluminum foil to help prevent entry of external contaminants.
Sterilize all formulas for 15 minutes at 15 psi.
Remove the medium as soon as the pressure reaches "0." Oversterilization or prolonged heating will change the composition of the medium. Sugars such as dextrose or malt can "caramelize," resulting in a medium that can inhibit mycelial growth and encourage sectors (mutations). Excessive heating of medium can also cause a drop in pH, resulting in a more acid medium. It is possible to destroy completely the gelling properties of agar by prolonged heating, and this destruction is hastened as the acidity increases.
For long-term health and maintenance of sacred strains, alternate any two of the above media. This will help prevent senescence, which can occur when cultures are grown on one medium only. Store strains at 35? - 40?F; transfer every six months. In this manner, sacred strains can be maintained for years.
Never compromise the integrity of your strain library. Strains showing even a hint of senescence should be removed from the collection.



 


--------------------
mushroom culture history making ...Mr. Allan is the best .....


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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: an idiot asking [Re: fugu]
    #1292675 - 02/09/03 07:51 AM (14 years, 1 month ago)

All you had to say was a "soy" , dextrose based agar fugu (sda s threw me off ) , I need no help with agar at all , was part I liked most and feel have mastered well enough for my needs , also too lazy to make own agar mixes, I buy them from scientific supply houses ! ADD water , cook up , pour , inoculate with fave spores . Lot less clean up , work , materials laying around house ............... easier to rotate type to aid in diversity .

15 min in pc huh ?
GOT LOTS OF CONTAM RATES I BET !


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


Edited by tripndicular (02/09/03 07:54 AM)


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Offlinefugu
Pooh-Bah

Registered: 01/06/03
Posts: 2,223
Loc: istanbul
Last seen: 6 years, 8 months
Re: an idiot asking [Re: tripndicular]
    #1292719 - 02/09/03 08:11 AM (14 years, 1 month ago)

thanks trip


--------------------
mushroom culture history making ...Mr. Allan is the best .....


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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: an idiot asking [Re: fugu]
    #1292761 - 02/09/03 08:24 AM (14 years, 1 month ago)

For what ?


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinefugu
Pooh-Bah

Registered: 01/06/03
Posts: 2,223
Loc: istanbul
Last seen: 6 years, 8 months
Re: an idiot asking [Re: tripndicular]
    #1292791 - 02/09/03 08:37 AM (14 years, 1 month ago)



--------------------
mushroom culture history making ...Mr. Allan is the best .....


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Amazon Shop for: ½ Pint Jars, Agar, Brown Rice Flour, Calcium Carbonate, Malt Extract, Potato Dextrose, pH Test Strips

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