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jba6511
addict
Registered: 11/04/02
Posts: 495
Last seen: 16 years, 3 months
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bad syringe?
#1288156 - 02/07/03 08:19 AM (21 years, 1 month ago) |
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I recently purchased 4 PES Hawaiian syringes from the little guy. I used the exact procedures used in MMGG, as I did in my first grow which was succesful. I did everything in a sterile room and did not even remove the tape from the syringe until time to innicluate. The jars were throughly cooled (overnight - about 12 hours). Also I had stored the syringes for 2 days in room tempature. It has been almost 2 weeks since Innoculation. Whats the problem? There has been no growth at all in any of my 20 jars. Also I live in Virginia if that helps.
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Room4Shroom
Registered: 01/15/03
Posts: 1,195
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Re: bad syringe? [Re: jba6511]
#1288160 - 02/07/03 08:26 AM (21 years, 1 month ago) |
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well you didn't mention anything about temperature...do you have something keeping the jars at around 85 degrees?
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Mycotopian
Mycotopian
Registered: 02/01/03
Posts: 158
Last seen: 21 years, 23 days
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No problem...Patients. It will come. I did my Hawiians over a week ago and still no sign of mycelium. I beleive these may not be very fast at colonizing. You don't have to have them at 85 degrees but it will help speed up the process. I did amazonian at the same time at they are about 20% colonized right now.
-------------------- "Hockey is a sport for white men. Basketball is a sport for black men. Golf is a sport for white men dressed like black pimps."
Edited by Mycotopian (02/07/03 08:48 AM)
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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I started some PES Hawaiians last week and they are almost fully colonized.
Incubation temps have a large roll in speed of growth.
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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BladeLSD
Squirrel Master
Registered: 08/24/00
Posts: 809
Last seen: 1 year, 4 months
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Jars dont have to be at 85 degrees. Incubating at 85 degrees is a new thing, atleast for me, I dont know if its faster(from experience), because the jars im currently incubating are growing at basically the same rate. When I started, before I knew of the shroomery I only knew of the PF site. I ordered from PF, and I followed PF to the T. The PF-tek suggest keeping the jars at 70 degrees, and that was my overall house temp, so i said fuck it and threw underneath my bed, and they colonized fine 3-4 weeks. Point being jars dont have to be at 85 degrees, so temperature is probably not his problem
-------------------- We might get glimpse's of objectivity every now, and then, but we're so inherently locked in our temporal and corporeal selves that we're irrevocably locked into subjectivity
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vatoloco
Puppet Hunter -DBK
Registered: 01/30/02
Posts: 7,653
Last seen: 19 years, 6 months
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Post deleted by MOE THE MAD SCIENTIST [Re: BladeLSD]
#1288314 - 02/07/03 09:30 AM (21 years, 1 month ago) |
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jba6511
addict
Registered: 11/04/02
Posts: 495
Last seen: 16 years, 3 months
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Re: bad syringe? [Re: vatoloco]
#1288509 - 02/07/03 10:32 AM (21 years, 1 month ago) |
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i have been keeping them in a cabinet that is small and the temp is around 74 - 78. Damn this little hobby is teaching me some real patience. Thanks for the help
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deanofmean
mycophagous
Registered: 12/06/02
Posts: 2,017
Loc: PNW
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Re: bad syringe? [Re: jba6511]
#1288559 - 02/07/03 10:50 AM (21 years, 1 month ago) |
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did you inoculate close to the side of the jar ? i wouldn't worry yet . patience is a virtue, and mycology is a good teacher .
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chills420
Poo Pie Maker
Registered: 01/01/03
Posts: 354
Last seen: 21 years, 1 month
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best thing to do when you take mycology as a hobby is to get another faster passed hobby along with it.
That or make alot of rye bags 2 pass the time. lol
-------------------- Teach a man to make cakes he will trip for a night. Teach a man to case he will trip forever
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Mycotopian
Mycotopian
Registered: 02/01/03
Posts: 158
Last seen: 21 years, 23 days
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Re: bad syringe? [Re: Roadkill]
#1288804 - 02/07/03 12:17 PM (21 years, 1 month ago) |
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Now you all got me worried because I inocuated Hawiians(from sporeworks) on 1/31 and I see no signs of mycelium. The jars have been kept at 83 degrees. I did the same number of jars with amazonian and they are taking off. I only used 1cc per jar, could this be why it is taking so long? On sporeworks website they have this in the description "Colonization times are fast, often consuming 1/2 pints of spawn in just under 13 days." It's been a week and I see no signs. Am I just getting impatient or do I have to be concerned?
-------------------- "Hockey is a sport for white men. Basketball is a sport for black men. Golf is a sport for white men dressed like black pimps."
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chills420
Poo Pie Maker
Registered: 01/01/03
Posts: 354
Last seen: 21 years, 1 month
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Heres a few of my newest project.
These are 5 days old at 12 pm saturday
Ya got to love rye
-------------------- Teach a man to make cakes he will trip for a night. Teach a man to case he will trip forever
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deanofmean
mycophagous
Registered: 12/06/02
Posts: 2,017
Loc: PNW
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Quote:
Now you all got me worried because I inocuated Hawiians(from sporeworks) on 1/31 and I see no signs of mycelium.
so, it's only been a week . take.a chill pill . sometimes it takes a little longer .
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ralphster44
collector
Registered: 01/03/01
Posts: 4,657
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Re: bad syringe? [Re: jba6511]
#1290289 - 02/08/03 04:58 AM (21 years, 1 month ago) |
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Post deleted by administrator.
-------------------- www.RalphstersSpores.com WE SHIP TO CANADA FROM WITHIN CANADA For your safety and security, we have a Secure Website. Also for your security, we will not take your credit card number. Your security and safety is of utmost importance to us.
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Mycotopian
Mycotopian
Registered: 02/01/03
Posts: 158
Last seen: 21 years, 23 days
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"so, it's only been a week . take.a chill pill . sometimes it takes a little longer." I am ok. I am not in a rush I just wonder how come they say the mycelium can fill the jar in 13 days and it has been 8 days for me in 83-84 degrees and out of ten jars and 4 incolutaon points on each jar I see not 1 spot on any jar. That's 40 points of inoculation and not one spot. I also inoculated 2 myco bags with ecuador (5cc each bag from spoeworks) and I see nothing in those bags either. I broke one bag up and left the other bag the way I received it. Please don't reply and tell me to be patient...I know this. I just want to know what the reason for the increase in time. (for both the myco bags and jars) Is it the spores?
-------------------- "Hockey is a sport for white men. Basketball is a sport for black men. Golf is a sport for white men dressed like black pimps."
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ralphster44
collector
Registered: 01/03/01
Posts: 4,657
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Post deleted by administrator.
-------------------- www.RalphstersSpores.com WE SHIP TO CANADA FROM WITHIN CANADA For your safety and security, we have a Secure Website. Also for your security, we will not take your credit card number. Your security and safety is of utmost importance to us.
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Mycotopian
Mycotopian
Registered: 02/01/03
Posts: 158
Last seen: 21 years, 23 days
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Just to give you some more info. The reason I have 2 myco bags is because the first bag I got did nothing. I wrote them for either replacement spores or bag preferably both. They sent me a new equador syringe and another myco bag. Now I have 2 myco bags with 5cc of equador in each. I inoculated these on 1/24. I will keep you posted on both (my 10 jars of hawiian done on 1/31 and my myco bags of hawiian done on 1/24). I will tell you one thing, I am getting the rest of my spores from TLG. If these I already have inoculated don't ever show mycelium, I won't even bother for replacements. I'll just move on
-------------------- "Hockey is a sport for white men. Basketball is a sport for black men. Golf is a sport for white men dressed like black pimps."
Edited by Mycotopian (02/08/03 06:04 AM)
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Mycotopian
Mycotopian
Registered: 02/01/03
Posts: 158
Last seen: 21 years, 23 days
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I didn't realize I was chatting with Ralphsters spores. I just went to your site and I see you have syringes for $10.00 (4 for $30.00) and you claim to have great spores. Sounds very good to me. I am going to wait and see if any grows in these before I place an order. How long does shipping usually take? I am in florida.
-------------------- "Hockey is a sport for white men. Basketball is a sport for black men. Golf is a sport for white men dressed like black pimps."
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ralphster44
collector
Registered: 01/03/01
Posts: 4,657
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Post deleted by administrator.
-------------------- www.RalphstersSpores.com WE SHIP TO CANADA FROM WITHIN CANADA For your safety and security, we have a Secure Website. Also for your security, we will not take your credit card number. Your security and safety is of utmost importance to us.
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Mycotopian
Mycotopian
Registered: 02/01/03
Posts: 158
Last seen: 21 years, 23 days
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All the spores in question came from sporeworks. The syringes did look pretty clear Thanks Ralphster. I be hooking up with you soon
-------------------- "Hockey is a sport for white men. Basketball is a sport for black men. Golf is a sport for white men dressed like black pimps."
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Mycotopian
Mycotopian
Registered: 02/01/03
Posts: 158
Last seen: 21 years, 23 days
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Do you think there was no spores at all or just a very little? Do you think anything is going to happen to any of those 10 jars? How common is it for someone to inoculate jars with spores from a reputable place and never have any mycelium growth? I have done many other jars (spores not from sporeworks) and never had "no mycelium growth".
-------------------- "Hockey is a sport for white men. Basketball is a sport for black men. Golf is a sport for white men dressed like black pimps."
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