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Invisible13shroomsM
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Registered: 01/01/09
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13(s) Steps to Easy Grow Bag Cultivation * 4
    #12903694 - 07/15/10 03:06 PM (13 years, 6 months ago)

https://www.shroomery.org/forums/showflat.php/Number/12903454

This is nothing new to shroomery but I dont see many using mycobags/growbags for easy cultivation.

My method is a combined version of several teks to make this happen.

First you need your supplies: (adjust your substrate mix ingredients according to species to be cultivated, this tek was made generally for P.Cubensis and its varieties).

LC/LI supplies:
8oz Glad plastic (PP5) mini rounds
Quart mason/ball/kerr jars w/lids
Drill & Drill Bits - 1/2" & 1/4"
Whatman Filters
Self healing injection ports
Silicone
Dextrose & Light Malt extract - LME (sugar in the raw/honey/karo can be substituted)
Water (for LCs/LIs and hydrating Coir brick)
Paper towels
Micropore tape
Pressure Cooker
Large 60cc syringe or a few 10-12cc syringes (for aspirating your myc from your LC jars)

General supplies:
Iso Alcohol
Latex/rubber gloves
StillAirbox or Flowhood (highly recommended, open air is for chumps)
Filter Patch Mycobag(s)
Clothes pins or tape or an impulse sealer (to close bags during PC & colonization)
Viable spore syringe or print (of your favorite fungi from your favorite shroomery vendor/sponsor to inoculate your agar)
Large Pot or 5gal bucket (to hydrate/mix substrate together)
Large serving spoon (optional to put substrate mix into mycobags)

Substrate Mix:
Grains of choice (wbs/rye berries/Oats/wheat)
Coir (manure can be used in place of or in addition to coir but requires proper pasteurization)
Vermiculite
Gypsum (optional)


1) Make your lids for your LC(s) and/or prep some pastey plates.





2) Make & PC your LC(s) and agar @ 15psi for 25mins (longer if using pda 30 to 45 mins).

3) Inoculate your LC(s) or LIs once cooled below 80*F (using a StillAirbox/flowhood)



While your LC is colonizing/near done (LI is already ready to use) its time to get your grains soaking and to hydrate your substrate mix.

4) Soak/prep your grains (a day b4 you make your substrate mix) the way you normally would (5-6qts of grains per block of coir used or per 8-9qts of manure/verm used).  I add gypsum to my grain soak for a lil Calcium boost. 

WBS prep
Rye Berrie prep

5) Put your coir in your pot/bucket and add 1gal of warm/hot water to it and let it swell/hydrate then add 1-2qts of verm and a cup of gypsum.  once the coir is fully hydrated and is all broke up you then add your 5-6qts of hydrated grain to the mix and combine very well  (Manure prep is a lil different, so prep manure separately to get to field capacity then add it to your grains minus the water and verm cuz its hydrated/mixed in already).

6) Get your filter patch bags and put 5-6qts of your hydrated substrate mix evenly in each bag till your mix is used up.  Wipe the inside sleeves out of any debris that might have got onto the top inside of the bags with an alcohol moist paper towel (for LC users put a bead of silicone next to the filter patch (smear it around for a moment, If you just leave the blob as is it will often dislodge very easily.  You now have a self healing injection port on the bag itself for LC inoculation later)).

7) Fold down the top of the bags till your near the filter patch and clip/pin/seal them closed.  (Dont cover/impede the filter patch in any way) PC/sterilize the bags for 4 hours @ 15psi (length of time needed to sterilize the center mass of each bag) or like RR does in these vids with woodlovers/edibles.  If you only have 1 mycobag to PC 90mins - 120mins @ 15psi is good.

8) Once your done PCing, hang or set your bags somewhere to cool/adjust to room temp (the bags will inflate as they sit so try not to touch them very much or you risk pulling in dirty air through the filter patch).

9) Now back to your LCs or LIs.  Use a new unopened or freshly sterilized syringe to extract your LC into your syringe(s) through the injection port. draw up/use 10-30cc of LC/LI for each bag to be inoculated.

10) Your sterile tek is important! You can do open air inoculation (not recommended) but a StillAirbox or flowhood is recommended for any and all inoculations.  Wipe surface of ports with iso alcohol and flame sterilize your needle every time you penetrate a port.  Inoculate each bag through the silicone port you added or through the top opening for LIs with 10-30cc then knead the bag very well to mix the LC/LI with your substrate (LI users need to seal/close the bag at this point) then put them somewhere where they will be undisturbed during colonization.

11) once fully colonized 100% you can fruit them in many ways, like remove the bag and place the colonized substrate block in a shotgun fc or in a martha/mini greenhouse right on the shelf or right in the bag itself, just roll down the sides as your mushies mature.  its all up to you on how you want to fruit them.

12) once you harvest/flush you can dunk/rehydrate the blocks for multiple flushes.

13) repeat steps 11 and 12 until the block is spent or contams are present then discard.


:aliendance:ENJOY!:aliendance:



Other links to the teks used to compile this tek;
https://www.shroomery.org/forums/showflat.php/Number/7507080
https://www.shroomery.org/forums/showflat.php/Number/11917410
https://www.shroomery.org/forums/showflat.php/Number/12826752
https://www.shroomery.org/forums/showflat.php/Number/11487687
http://www.mushroomvideos.com/Horse-Manure-Substrate
http://www.mushroomvideos.com/Grain-Preparation



:scaryshroom::thumbup:


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OfflineYaYoKush
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Re: Easy Mycobag Bulk Cultivation [Re: 13shrooms]
    #12903708 - 07/15/10 03:08 PM (13 years, 6 months ago)

Nice work


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are we wizards? lol
...we do deal in various magics


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InvisibleEvilMushroom666
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Re: Easy Mycobag Bulk Cultivation [Re: 13shrooms]
    #12903724 - 07/15/10 03:11 PM (13 years, 6 months ago)

Nice write up. +5 :mushroom2:

I can see this helping out a lot of people.


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InvisibleDragonaut


Registered: 06/24/04
Posts: 6,190
Re: Easy Mycobag Bulk Cultivation [Re: YaYoKush]
    #12903727 - 07/15/10 03:12 PM (13 years, 6 months ago)

:nothingtoadd:


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OfflineBreakfast Crew
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Re: Easy Mycobag Bulk Cultivation [Re: YaYoKush]
    #12903729 - 07/15/10 03:12 PM (13 years, 6 months ago)

You are stealing my glory :shakingfist:.


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OfflineDamion5050
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Re: Easy Mycobag Bulk Cultivation [Re: Dragonaut]
    #12903732 - 07/15/10 03:13 PM (13 years, 6 months ago)

I do gayest post you every posted hahahaha




Nice Write Up.


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Invisible13shroomsM
Lightning Shaman
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Re: Easy Mycobag Bulk Cultivation [Re: Breakfast Crew]
    #12904847 - 07/15/10 09:50 PM (13 years, 6 months ago)

Quote:

Breakfast Crew said:
You are stealing my glory :shakingfist:.




:crazybeard:

Wait, what? :stoned2:


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Invisible13shroomsM
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Re: Easy Mycobag Bulk Cultivation [Re: 13shrooms]
    #12918149 - 07/19/10 01:25 AM (13 years, 6 months ago)

:bump:


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OfflineDamion5050
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Re: Easy Mycobag Bulk Cultivation [Re: 13shrooms]
    #12919957 - 07/19/10 02:55 PM (13 years, 6 months ago)

:tardhi:


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Invisible13shroomsM
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Re: Easy Mycobag Bulk Cultivation [Re: Damion5050]
    #12932768 - 07/21/10 09:22 PM (13 years, 6 months ago)

:stoner:


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InvisibleShea25
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Re: Easy Mycobag Bulk Cultivation [Re: 13shrooms]
    #12932794 - 07/21/10 09:26 PM (13 years, 6 months ago)

:intardnet:


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Invisible13shroomsM
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Registered: 01/01/09
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Re: Easy Mycobag Bulk Cultivation [Re: Shea25]
    #12944397 - 07/24/10 06:58 AM (13 years, 6 months ago)

Hmmmm,  I posted this tek on shroomtopia and they deleted it and my acct. ? .  :shrug: 
:ohwell: their loss.


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Invisibletruskool
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Re: Easy Mycobag Bulk Cultivation [Re: Damion5050]
    #12944538 - 07/24/10 08:31 AM (13 years, 6 months ago)

Quote:

Damion5050 said:
I do gayest post you every posted hahahaha




Nice Write Up.


:lmafo:


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Offlinehamloaf
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Re: Easy Mycobag Bulk Cultivation [Re: 13shrooms]
    #12944546 - 07/24/10 08:35 AM (13 years, 6 months ago)

Quote:

13shrooms said:
Hmmmm,  I posted this tek on shroomtopia and they deleted it and my acct. ? .  :shrug: 
:ohwell: their loss.



Seriously?


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How I Get Stuff done. - My Reference Guide. - My Grows.


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Invisibletruskool
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Re: Easy Mycobag Bulk Cultivation [Re: hamloaf]
    #12944572 - 07/24/10 08:43 AM (13 years, 6 months ago)

Quote:

hamloaf said:
Quote:

13shrooms said:
Hmmmm,  I posted this tek on shroomtopia and they deleted it and my acct. ? .  :shrug: 
:ohwell: their loss.



Seriously?



Yes seriously, but there is a little more to the story.


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

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Invisible13shroomsM
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Re: Easy Mycobag Bulk Cultivation [Re: truskool]
    #12944621 - 07/24/10 09:07 AM (13 years, 6 months ago)

not on my end, I didnt get involved except for knowing you guys and coming from the shroomery. :shrug:

its OK, Im over it. :grin:


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Invisibletruskool
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Re: Easy Mycobag Bulk Cultivation [Re: 13shrooms]
    #12944637 - 07/24/10 09:14 AM (13 years, 6 months ago)

Quote:

13shrooms said:
not on my end, I didnt get involved except for knowing you guys and coming from the shroomery. :shrug:

its OK, Im over it. :grin:



Yea I meant to say that.  It is totally there loss.  They lost enough ppl that could have been great additions


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Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

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Offlinehamloaf
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Re: Easy Mycobag Bulk Cultivation [Re: truskool]
    #12944643 - 07/24/10 09:16 AM (13 years, 6 months ago)

I like the advise of the shroomery better.
HL


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Invisible13shroomsM
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Re: Easy Mycobag Bulk Cultivation [Re: hamloaf]
    #12944717 - 07/24/10 09:45 AM (13 years, 6 months ago)

Quote:

hamloaf said:
I like the advise of the shroomery better.
HL




:headbang3:
Its where I learned everything I know about mycology. :respect:


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Offlinek00laid
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Re: Easy Mycobag Bulk Cultivation [Re: 13shrooms]
    #12944774 - 07/24/10 10:08 AM (13 years, 6 months ago)

never been anywhere else :datass:


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