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Offlinec-ray
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Long-term Maintenance of Fungal Cultures on Perlite in Cryovials – an Alternative for Agar Slants
    #12824878 - 06/29/10 05:17 PM (13 years, 7 months ago)

full text: http://www.cssm.info/priloha/fm2008_534.pdf

Quote:

Institute of Microbiology, v.v.i., Academy of Sciences of the Czech Republic, 142 20 Prague, Czech Republic
e-mail homolka@biomed.cas.cz

Received 22 August 2008
Revised version 20 October 2008

ABSTRACT. Cultures of 33 basidiomycete strains out of 35 tested were viable with unchanged characteristics after four years of maintenance on perlite in cryovials. These cultures can be a good substitute for agar cultures in long-term maintenance of fungi. For comparison, the storage under oil was evaluated but it turned out to be unsuitable for the majority of our cultures.




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Invisiblewygram
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Re: Long-term Maintenance of Fungal Cultures on Perlite in Cryovials – an Alternative for Agar Slants [Re: c-ray]
    #12826372 - 06/29/10 10:10 PM (13 years, 7 months ago)

This is definitely interesting. :thumbup:


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Offlinesolumvita
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Re: Long-term Maintenance of Fungal Cultures on Perlite in Cryovials – an Alternative for Agar Slants [Re: wygram]
    #12827287 - 06/30/10 03:52 AM (13 years, 7 months ago)

nice find, always good to know of alternative ideas.


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Offlineukshroomer
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Re: Long-term Maintenance of Fungal Cultures on Perlite in Cryovials – an Alternative for Agar Slants [Re: wygram]
    #12827610 - 06/30/10 08:07 AM (13 years, 7 months ago)

Quote:

wygram said:
This is definitely interesting. :thumbup:



definitely . some one should try this out. i am assuming by cryovials the cultures are frozen???? or are they refrigerated?


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Offlinesolumvita
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Re: Long-term Maintenance of Fungal Cultures on Perlite in Cryovials – an Alternative for Agar Slants [Re: ukshroomer]
    #12827654 - 06/30/10 08:24 AM (13 years, 7 months ago)

stored at 4 deg C


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OfflineNizzyJones
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Re: Long-term Maintenance of Fungal Cultures on Perlite in Cryovials – an Alternative for Agar Slants [Re: ukshroomer]
    #12828736 - 06/30/10 01:17 PM (13 years, 7 months ago)

So roughly fridge temperature.

And cryotubes are just a brand of plastic threaded test tubes.


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Offlinefalcon
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Re: Long-term Maintenance of Fungal Cultures on Perlite in Cryovials – an Alternative for Agar Slants [Re: c-ray]
    #12849126 - 07/04/10 08:03 PM (13 years, 7 months ago)

Hey, thanks for posting that.


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Offlinec-ray
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Re: Long-term Maintenance of Fungal Cultures on Perlite in Cryovials – an Alternative for Agar Slants [Re: falcon]
    #12856739 - 07/06/10 02:06 PM (13 years, 7 months ago)

here's a long term storage technique using pieces of filter paper:
http://blog.mycology.cornell.edu/?p=383


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Invisiblefastfred
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Re: Long-term Maintenance of Fungal Cultures on Perlite in Cryovials – an Alternative for Agar Slants [Re: c-ray]
    #12858019 - 07/06/10 06:07 PM (13 years, 7 months ago)

That's more of a spore preservation method as anything.  I see they let it sporulate before storage, so you're not really recovering your pure culture, you get a mix of your culture, spores, and progeny.  Even though that's not a problem with fruiting mushrooms, since they won't sporulate on the paper, it isn't an advancement since people have been using paper spawn for decades.


-FF


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Offlinewbastz
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Re: Long-term Maintenance of Fungal Cultures on Perlite in Cryovials – an Alternative for Agar Slants [Re: c-ray]
    #12861346 - 07/07/10 10:47 AM (13 years, 7 months ago)

Hi C-ray,

Excuse me, but I don´t know exactly what is a wort or how to prepare it.

Is it a kind of broth used for brewing ? How to prepare a wort 4º Bailling ?

Could I use light malt extract or something like that ?

If so, how much malt extract would be added to the agar for an equivalent recipe ?

Any help will be welcome.

Cheers,

Wilson


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Offlinefalcon
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Re: Long-term Maintenance of Fungal Cultures on Perlite in Cryovials – an Alternative for Agar Slants [Re: wbastz]
    #12864333 - 07/07/10 09:34 PM (13 years, 7 months ago)

It's not Bailling it's Balling and it's a measure of specific gravity used in beer making. You could use malt extract, but I don't know how much malt extract you would use to get 4 degrees Balling.


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Offlinewbastz
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Re: Long-term Maintenance of Fungal Cultures on Perlite in Cryovials – an Alternative for Agar Slants [Re: falcon]
    #12866070 - 07/08/10 06:41 AM (13 years, 7 months ago)

Thanks Falcon,

It was just a typo. I will test two compositions: the first with 1.7% of malt extract and the second with 3.0% of malt extract and 0.3% of soy peptone and see what happens.

Cheers,

Wilson


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Offlinewbastz
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Re: Long-term Maintenance of Fungal Cultures on Perlite in Cryovials – an Alternative for Agar Slants [Re: falcon]
    #12866097 - 07/08/10 06:57 AM (13 years, 7 months ago)

Information on correlations between degrees Balling ,degrees Plato , degrees Brix and specific gravity :

http://www.brewingtechniques.com/library/backissues/issue1.3/manning.html


Cheers,

Wilson


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Invisibleteknix
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Re: Long-term Maintenance of Fungal Cultures on Perlite in Cryovials – an Alternative for Agar Slants [Re: wbastz]
    #12878882 - 07/10/10 05:30 PM (13 years, 7 months ago)



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InvisibleMonkeyKnifeFight
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Re: Long-term Maintenance of Fungal Cultures on Perlite in Cryovials – an Alternative for Agar Slants [Re: teknix]
    #12881709 - 07/11/10 09:59 AM (13 years, 7 months ago)

I never paid too much attention to specific gravity and all that but wort is pretty easy to make.  For a big batch of beer we would grind malted barley (two row) in a grain mill, heat a bunch of water to 170F, mix it with the grain and it should stabilize to ~160F.  Then just cover it and leave it alone for an hour or so and drain off the liquid and you have wort.  It'll be dark and really sweet.  We would use 20+lbs of grain for 10-12 gallons of beer.

Not clear on what you guys want to use it for but that's how you make wort from scratch.  Extract should basically get you to the same thing.


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