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Offlinejba6511
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Registered: 11/04/02
Posts: 495
Last seen: 8 years, 11 months
bad syringe?
    #1288156 - 02/07/03 10:19 AM (13 years, 9 months ago)

I recently purchased 4 PES Hawaiian syringes from the little guy. I used the exact procedures used in MMGG, as I did in my first grow which was succesful. I did everything in a sterile room and did not even remove the tape from the syringe until time to innicluate. The jars were throughly cooled (overnight - about 12 hours). Also I had stored the syringes for 2 days in room tempature. It has been almost 2 weeks since Innoculation. Whats the problem? There has been no growth at all in any of my 20 jars. Also I live in Virginia if that helps.


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InvisibleRoom4Shroom
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Registered: 01/15/03
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Re: bad syringe? [Re: jba6511]
    #1288160 - 02/07/03 10:26 AM (13 years, 9 months ago)

well you didn't mention anything about temperature...do you have something keeping the jars at around 85 degrees?


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OfflineMycotopian
Mycotopian

Registered: 02/01/03
Posts: 158
Last seen: 13 years, 8 months
Re: bad syringe? [Re: Room4Shroom]
    #1288207 - 02/07/03 10:47 AM (13 years, 9 months ago)

No problem...Patients. It will come. I did my Hawiians over a week ago and still no sign of mycelium. I beleive these may not be very fast at colonizing. You don't have to have them at 85 degrees but it will help speed up the process. I did amazonian at the same time at they are about 20% colonized right now.


--------------------
"Hockey is a sport for white men. Basketball is a sport for black men. Golf is a sport for white men dressed like black pimps."





Edited by Mycotopian (02/07/03 10:48 AM)


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InvisibleRoadkill
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Registered: 12/11/01
Posts: 22,598
Loc: Snoqualmie, Wa.
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Re: bad syringe? [Re: Mycotopian]
    #1288220 - 02/07/03 10:53 AM (13 years, 9 months ago)

I started some PES Hawaiians last week and they are almost fully colonized.

Incubation temps have a large roll in speed of growth.



--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineBladeLSD
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Registered: 08/25/00
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Re: bad syringe? [Re: Room4Shroom]
    #1288243 - 02/07/03 11:03 AM (13 years, 9 months ago)

Jars dont have to be at 85 degrees. Incubating at 85 degrees is a new thing, atleast for me, I dont know if its faster(from experience), because the jars im currently incubating are growing at basically the same rate. When I started, before I knew of the shroomery I only knew of the PF site. I ordered from PF, and I followed PF to the T. The PF-tek suggest keeping the jars at 70 degrees, and that was my overall house temp, so i said fuck it and threw underneath my bed, and they colonized fine 3-4 weeks. Point being jars dont have to be at 85 degrees, so temperature is probably not his problem


--------------------
We might get glimpse's of objectivity every now, and then, but we're so inherently locked in our temporal and corporeal selves that we're irrevocably locked into subjectivity :crazy:


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Offlinevatoloco
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Registered: 01/30/02
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Post deleted by MOE THE MAD SCIENTIST [Re: BladeLSD]
    #1288314 - 02/07/03 11:30 AM (13 years, 9 months ago)



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Offlinejba6511
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Registered: 11/04/02
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Re: bad syringe? [Re: vatoloco]
    #1288509 - 02/07/03 12:32 PM (13 years, 9 months ago)

i have been keeping them in a cabinet that is small and the temp is around 74 - 78.
Damn this little hobby is teaching me some real patience. Thanks for the help


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Invisibledeanofmean
mycophagous

Registered: 12/07/02
Posts: 2,017
Loc: PNW
Re: bad syringe? [Re: jba6511]
    #1288559 - 02/07/03 12:50 PM (13 years, 9 months ago)

did you inoculate close to the side of the jar ?
i wouldn't worry yet .
patience is a virtue, and mycology is a good teacher . :smirk:


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Offlinechills420
Poo Pie Maker

Registered: 01/01/03
Posts: 354
Last seen: 13 years, 9 months
Re: bad syringe? [Re: deanofmean]
    #1288564 - 02/07/03 12:53 PM (13 years, 9 months ago)

best thing to do when you take mycology as a hobby is to get another faster passed hobby along with it.

That or make alot of rye bags 2 pass the time.
lol :grin: 


--------------------
Teach a man to make cakes he will trip for a night. Teach a man to case he will trip forever


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OfflineMycotopian
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Registered: 02/01/03
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Last seen: 13 years, 8 months
Re: bad syringe? [Re: Roadkill]
    #1288804 - 02/07/03 02:17 PM (13 years, 9 months ago)

Now you all got me worried because I inocuated Hawiians(from sporeworks) on 1/31 and I see no signs of mycelium. The jars have been kept at 83 degrees. I did the same number of jars with amazonian and they are taking off. I only used 1cc per jar, could this be why it is taking so long? On sporeworks website they have this in the description "Colonization times are fast, often consuming 1/2 pints of spawn in just under 13 days." It's been a week and I see no signs. Am I just getting impatient or do I have to be concerned?


--------------------
"Hockey is a sport for white men. Basketball is a sport for black men. Golf is a sport for white men dressed like black pimps."





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Offlinechills420
Poo Pie Maker

Registered: 01/01/03
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Re: bad syringe? [Re: Mycotopian]
    #1289495 - 02/07/03 06:54 PM (13 years, 9 months ago)

Heres a few of my newest project.

These are 5 days old at 12 pm saturday







Ya got to love rye



--------------------
Teach a man to make cakes he will trip for a night. Teach a man to case he will trip forever


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Invisibledeanofmean
mycophagous

Registered: 12/07/02
Posts: 2,017
Loc: PNW
Re: bad syringe? [Re: Mycotopian]
    #1289590 - 02/07/03 07:55 PM (13 years, 9 months ago)

Quote:

Now you all got me worried because I inocuated Hawiians(from sporeworks) on 1/31 and I see no signs of mycelium.



so, it's only been a week . take.a chill pill . :smile: sometimes it takes a little longer . 


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Invisibleralphster44
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Re: bad syringe? [Re: jba6511]
    #1290289 - 02/08/03 06:58 AM (13 years, 9 months ago)

Post deleted by administrator.


--------------------
www.RalphstersSpores.com

WE SHIP TO CANADA FROM WITHIN CANADA :smile:

For your safety and security, we have a Secure Website.
Also for your security, we will not take your credit card number.
Your security and safety is of utmost importance to us.


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OfflineMycotopian
Mycotopian

Registered: 02/01/03
Posts: 158
Last seen: 13 years, 8 months
Re: bad syringe? [Re: deanofmean]
    #1290349 - 02/08/03 07:35 AM (13 years, 9 months ago)

"so, it's only been a week . take.a chill pill . sometimes it takes a little longer."
I am ok. I am not in a rush I just wonder how come they say the mycelium can fill the jar in 13 days and it has been 8 days for me in 83-84 degrees and out of ten jars and 4 incolutaon points on each jar I see not 1 spot on any jar. That's 40 points of inoculation and not one spot. I also inoculated 2 myco bags with ecuador (5cc each bag from spoeworks) and I see nothing in those bags either. I broke one bag up and left the other bag the way I received it.
Please don't reply and tell me to be patient...I know this. I just want to know what the reason for the increase in time. (for both the myco bags and jars) Is it the spores?


--------------------
"Hockey is a sport for white men. Basketball is a sport for black men. Golf is a sport for white men dressed like black pimps."





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Invisibleralphster44
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Re: bad syringe? [Re: Mycotopian]
    #1290355 - 02/08/03 07:38 AM (13 years, 9 months ago)

Post deleted by administrator.


--------------------
www.RalphstersSpores.com

WE SHIP TO CANADA FROM WITHIN CANADA :smile:

For your safety and security, we have a Secure Website.
Also for your security, we will not take your credit card number.
Your security and safety is of utmost importance to us.


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OfflineMycotopian
Mycotopian

Registered: 02/01/03
Posts: 158
Last seen: 13 years, 8 months
Re: bad syringe? [Re: ralphster44]
    #1290393 - 02/08/03 08:01 AM (13 years, 9 months ago)

Just to give you some more info. The reason I have 2 myco bags is because the first bag I got did nothing. I wrote them for either replacement spores or bag preferably both. They sent me a new equador syringe and another myco bag. Now I have 2 myco bags with 5cc of equador in each. I inoculated these on 1/24. I will keep you posted on both (my 10 jars of hawiian done on 1/31 and my myco bags of hawiian done on 1/24).
I will tell you one thing, I am getting the rest of my spores from TLG. If these I already have inoculated don't ever show mycelium, I won't even bother for replacements. I'll just move on :grin: 


--------------------
"Hockey is a sport for white men. Basketball is a sport for black men. Golf is a sport for white men dressed like black pimps."





Edited by Mycotopian (02/08/03 08:04 AM)


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OfflineMycotopian
Mycotopian

Registered: 02/01/03
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Last seen: 13 years, 8 months
Re: bad syringe? [Re: ralphster44]
    #1290413 - 02/08/03 08:12 AM (13 years, 9 months ago)

I didn't realize I was chatting with Ralphsters spores. I just went to your site and I see you have syringes for $10.00 (4 for $30.00) and you claim to have great spores. Sounds very good to me. I am going to wait and see if any grows in these before I place an order. How long does shipping usually take? I am in florida.


--------------------
"Hockey is a sport for white men. Basketball is a sport for black men. Golf is a sport for white men dressed like black pimps."





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Invisibleralphster44
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Re: bad syringe? [Re: Mycotopian]
    #1290430 - 02/08/03 08:22 AM (13 years, 9 months ago)

Post deleted by administrator.


--------------------
www.RalphstersSpores.com

WE SHIP TO CANADA FROM WITHIN CANADA :smile:

For your safety and security, we have a Secure Website.
Also for your security, we will not take your credit card number.
Your security and safety is of utmost importance to us.


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OfflineMycotopian
Mycotopian

Registered: 02/01/03
Posts: 158
Last seen: 13 years, 8 months
Re: bad syringe? [Re: ralphster44]
    #1290445 - 02/08/03 08:29 AM (13 years, 9 months ago)

All the spores in question came from sporeworks. The syringes did look pretty clear  :frown: Thanks Ralphster. I be hooking up with you soon  :grin: 


--------------------
"Hockey is a sport for white men. Basketball is a sport for black men. Golf is a sport for white men dressed like black pimps."





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OfflineMycotopian
Mycotopian

Registered: 02/01/03
Posts: 158
Last seen: 13 years, 8 months
Re: bad syringe? [Re: Mycotopian]
    #1290528 - 02/08/03 09:00 AM (13 years, 9 months ago)

Do you think there was no spores at all or just a very little? Do you think anything is going to happen to any of those 10 jars? How common is it for someone to inoculate jars with spores from a reputable place and never have any mycelium growth? I have done many other jars (spores not from sporeworks) and never had "no mycelium growth".


--------------------
"Hockey is a sport for white men. Basketball is a sport for black men. Golf is a sport for white men dressed like black pimps."





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Amazon Shop for: ½ Pint Jars, Rye Grain

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