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Invisiblestonesun
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Re: My Elementary Liquid Culture (LC) Tek [Re: Damion5050]
    #12831519 - 06/30/10 09:22 PM (13 years, 8 months ago)

Hah, that's surprising.
Eel is usually the favorite for even non-sushi eaters
because it's cooked and kinda barbecue flavored.
Oh well, to each their own.
I'm glad that you tried and liked it.

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OfflineDamion5050
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Re: My Elementary Liquid Culture (LC) Tek [Re: stonesun]
    #12831552 - 06/30/10 09:29 PM (13 years, 8 months ago)

:shrug:  I don't know lol.  I did have some swordfish a couple months back on vacation that was like sliced into a 2 inch thick steak.  Man was that shit good.  I got it down in Cape Coral Florida..  Shit was crazy good.  I been trying to get some swordfish up here in my neck of the woods and try cooking me another swordfish steak.

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InvisibleCH HELL
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Re: My Elementary Liquid Culture (LC) Tek [Re: stonesun]
    #12831751 - 06/30/10 10:02 PM (13 years, 8 months ago)

Quote:

stonesun said:
Damion and CH, may I ask why you guy are using LCs?
I know you both use agar, therefore I don't really see the point of an LC.
Is it because it's quicker than let's say agar->grain->GLC?
I don't see any other advantages compared to agar.
When I rarely make a LC (just for edibles), I inoculate that with an agar wedge too.






I do alot of agar-g2g,  but I will use to make LC's to spread cultures.  I do a little of every thing and a lot of edibles and I can make new master jars fast when I have a proven LC.
Here is a pic of a few cultures of mine that are growing.
CH

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Invisiblestonesun
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Re: My Elementary Liquid Culture (LC) Tek [Re: CH HELL]
    #12832304 - 06/30/10 11:45 PM (13 years, 8 months ago)

Yeah CH, on your part I figured it's mostly for edibles.
I trade edible cultures pretty often as well, and most people ask for LC
rather than agar wedge.

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Re: My Elementary Liquid Culture (LC) Tek [Re: stonesun]
    #12833236 - 07/01/10 06:31 AM (13 years, 8 months ago)

What about those lids? where can I get some


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OfflineMad_Hatter2004
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Re: My Elementary Liquid Culture (LC) Tek [Re: talkingwalnut]
    #12833241 - 07/01/10 06:34 AM (13 years, 8 months ago)

Quote:

talkingwalnut said:
What about those lids? where can I get some




http://themadseason.com/index.php?main_page=index&cPath=7&zenid=q5o0us857g9eqhtdj2tt4r6e00


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7 days without waves makes one weak!

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Re: My Elementary Liquid Culture (LC) Tek [Re: Mad_Hatter2004]
    #12833264 - 07/01/10 06:49 AM (13 years, 8 months ago)

thanks man


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OfflineMad_Hatter2004
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Re: My Elementary Liquid Culture (LC) Tek [Re: talkingwalnut]
    #12833272 - 07/01/10 06:53 AM (13 years, 8 months ago)

No problemo brother!


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7 days without waves makes one weak!

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OfflineDamion5050
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Re: My Elementary Liquid Culture (LC) Tek [Re: Mad_Hatter2004]
    #12834580 - 07/01/10 01:25 PM (13 years, 8 months ago)

As already posted u can buy those lids from the mad season and or make them.  Just depends on how hands on you want to be.

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Re: My Elementary Liquid Culture (LC) Tek [Re: Damion5050]
    #12834882 - 07/01/10 02:28 PM (13 years, 8 months ago)

how long can you store LC"s?


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Invisiblehamloaf
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Re: My Elementary Liquid Culture (LC) Tek [Re: talkingwalnut]
    #12835171 - 07/01/10 03:38 PM (13 years, 8 months ago)

2 months on the shelf at room temp, topps.

Meanwhile your mycelium is becoming less and less viable.
As it consumes the media and time goes on.

In the fridge.
Same story.

Only drawn out over a longer period of time.

Don't store your DEX/LME LC in the fridge.
For longer than 6 months.

It's advise-able.
That once your LC's are fully colonized.

To aspire them immediately.
Then inject into your medium of choice.

Grains, agar, more liquid media, brown rice flour cakes, or Grain LC.

This procedure/regimen of when to aspire your LC's and inoculate.
Will insure you have your liquid culture at it's most vigorous.


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

Edited by LiquidMyce (07/01/10 03:50 PM)

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OfflineeVenom
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Re: My Elementary Liquid Culture (LC) Tek [Re: hamloaf]
    #12835657 - 07/01/10 05:31 PM (13 years, 8 months ago)

I have had LC for more that 18months that are still very vigorous!

also Grain LC in a vacutainer can last for more than 10 years some people  say that will last more that 20


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eVenom

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RR

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OfflineDamion5050
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Re: My Elementary Liquid Culture (LC) Tek [Re: eVenom]
    #12835873 - 07/01/10 06:13 PM (13 years, 8 months ago)

For long term storage of a strain I take 10mL of a LC and inject it into a vacutainer and place it in the fridge.

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Invisiblehamloaf
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Re: My Elementary Liquid Culture (LC) Tek [Re: Damion5050]
    #12837424 - 07/01/10 10:55 PM (13 years, 8 months ago)

Freshly germinated Pink Oyster Liquid Culture.
Only four days after inoculation.
Using 1.5 tablespoons LME/DEX per quart or 400ml of distilled water.



Yellow Oyster liquid.
Inoculated with an agar wedge.
4 days ago.
Using 1.5 tablespoons LME/DEX per quart or 400ml of distilled water.
With 0.4th a gram nutritional yeast per quart or 400ml of distilled water.



--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

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Re: My Elementary Liquid Culture (LC) Tek [Re: hamloaf] * 1
    #12842035 - 07/02/10 09:24 PM (13 years, 8 months ago)

Looks good ham..

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Re: My Elementary Liquid Culture (LC) Tek [Re: Damion5050]
    #12842050 - 07/02/10 09:27 PM (13 years, 8 months ago)

:awesome:


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OfflineDamion5050
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Re: My Elementary Liquid Culture (LC) Tek [Re: Dragonaut]
    #12842096 - 07/02/10 09:36 PM (13 years, 8 months ago)

Here are some pictures of my 3 LC's that I used in the tek.  It is what 3 days in and got really good growth already.


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InvisibleShea25
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Re: My Elementary Liquid Culture (LC) Tek [Re: Damion5050]
    #12842116 - 07/02/10 09:39 PM (13 years, 8 months ago)

My next experiment is with Sugar in the raw, but I must say as much as karo gets bashed it has always worked well for me, with yeast




Day 5


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OfflineAlex TrebekS
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Re: My Elementary Liquid Culture (LC) Tek [Re: Damion5050]
    #12842434 - 07/02/10 10:38 PM (13 years, 8 months ago)

I pretty much follow the same tek to a t except for the fact I don't use Dextrose.  Does this make much of a difference ?
Mine seem to do excellent with out it and I prefer the light malt over honey any day.

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Re: My Elementary Liquid Culture (LC) Tek [Re: Damion5050]
    #12843698 - 07/03/10 05:22 AM (13 years, 8 months ago)

LC show and tell. :grin:




Here goes a shot of a Chilean LC.
With the heaping.
Full tablespoon of LME/DEX.
Per quart distilled of water.
At 70-80 percent colonization.



(Edit add)

Guys,


When taking a picture of an LC.
Hang a black or dark colored drop cloth.
Place your LC in front of the drop cloth.
Use a 6500K light to illuminate your picture.

The light spectrum and the dark colored backgroung.
Really stand out your LC.
When the light passes through it.
Against the dark drop cloth background.

As in the Chilean LC above.^
Chilean. LC's and a 5 Quart Grain Bag of Wild Bird Seed.

Edited by LiquidMyce (07/03/10 08:06 AM)

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