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Offlinecelestialtripper
Test Pilot
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Registered: 08/02/02
Posts: 272
Last seen: 8 years, 6 months
colonize damn you!
    #1281924 - 02/05/03 12:34 PM (21 years, 1 month ago)

well, its been almost a month since ive started some new cakes, 5 are going pretty strong with mycelium growth (id say around 75%-80% colonized), while another 5 are showing absolutely no signs of colonization. all of the cakes were prepared at the exact same time, the exact same way. sterilized at the exact same time for the same amount of time, left to cool for the exact amount of time and then inoculated at the same time. there are absolutely no differences in them except for that 5 of the cakes just arnt colonizing, theres just no mycelium growing on them. they are all being stored in the same container, bah! i just dont understand why if they have all had the exact same thing done to them as the other 5 cakes why these other 5 wont get their asses going!! any ideas as to whats going on here?
thanks,
.cel.

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OfflineMycotopian
Mycotopian

Registered: 02/01/03
Posts: 158
Last seen: 21 years, 22 days
Re: colonize damn you! [Re: celestialtripper]
    #1281932 - 02/05/03 12:37 PM (21 years, 1 month ago)

How much spores did you put in each jar? 1cc?


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"Hockey is a sport for white men. Basketball is a sport for black men. Golf is a sport for white men dressed like black pimps."




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Offlineevilacid
PotHead

Registered: 01/26/03
Posts: 193
Loc: Amsterdam,Netherlands
Last seen: 20 years, 11 months
Re: colonize damn you! [Re: Mycotopian]
    #1281935 - 02/05/03 12:38 PM (21 years, 1 month ago)

first time growing for me been 3 days i got growth check the grow log forum there can be many things your not doing to make it grow u can try cold shocking it

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OfflinePooGrower
Mr. Hanky

Registered: 11/01/02
Posts: 542
Loc: East Side
Last seen: 18 years, 26 days
Re: colonize damn you! [Re: evilacid]
    #1281970 - 02/05/03 12:45 PM (21 years, 1 month ago)

did you use 2 different strains?


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"I WONT STOP TILL I HAVE A LIFE TIME SUPPLY" "dont be offended by anything i say, its in a joking manner"

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Offlineevilacid
PotHead

Registered: 01/26/03
Posts: 193
Loc: Amsterdam,Netherlands
Last seen: 20 years, 11 months
Re: colonize damn you! [Re: PooGrower]
    #1281973 - 02/05/03 12:47 PM (21 years, 1 month ago)

i used 1

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Offlinecelestialtripper
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Registered: 08/02/02
Posts: 272
Last seen: 8 years, 6 months
Re: colonize damn you! [Re: Mycotopian]
    #1281974 - 02/05/03 12:47 PM (21 years, 1 month ago)

1cc a jar, through 5 diff. holes in each lid, same strain.

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InvisibleSubGen1us

Registered: 11/26/02
Posts: 3,427
Re: colonize damn you! [Re: celestialtripper]
    #1281975 - 02/05/03 12:47 PM (21 years, 1 month ago)

If u mixed all the stuff up in one big bowl the flour tends to goto the bottom and make the substrates of each jar differnt when filling.

If thats not the case maybe u got some bacterial contam that stoped the myc from growing. open 1 and u know from the smell.


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OfflineAngry Mycologist
Spontaneouslycombusting

Registered: 11/24/02
Posts: 1,282
Loc: Galapagos
Last seen: 20 years, 10 months
Re: colonize damn you! [Re: celestialtripper]
    #1282060 - 02/05/03 01:12 PM (21 years, 1 month ago)

What are your incubation temps?


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The proper penalty of ignorance, which is of course that those who don't know should learn from those who do... - Plato

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OfflinePooGrower
Mr. Hanky

Registered: 11/01/02
Posts: 542
Loc: East Side
Last seen: 18 years, 26 days
Re: colonize damn you! [Re: Angry Mycologist]
    #1282118 - 02/05/03 01:28 PM (21 years, 1 month ago)

did you flame sterizlie the tip of the needle after each jar? could be a bad syringe, or unsterile syringe


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"I WONT STOP TILL I HAVE A LIFE TIME SUPPLY" "dont be offended by anything i say, its in a joking manner"

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InvisibleSixCee
keep rolling
Male

Registered: 06/12/02
Posts: 3,720
Loc: US, Chicago
Re: colonize damn you! [Re: PooGrower]
    #1282176 - 02/05/03 01:38 PM (21 years, 1 month ago)

Did you sterilzie in a pot of boiling water? Maybe the jars on the bottom dried out.


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Offlinecelestialtripper
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Registered: 08/02/02
Posts: 272
Last seen: 8 years, 6 months
Re: colonize damn you! [Re: celestialtripper]
    #1282596 - 02/05/03 03:41 PM (21 years, 1 month ago)

everything was mixed in one bowl yes, but i really, really mixed everything up, broke up all the chunks, ect.
syringe was flame sterilized and yes the jars were sterilized in a pot of poiling water not a pc.

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InvisibleSubGen1us

Registered: 11/26/02
Posts: 3,427
Re: colonize damn you! [Re: celestialtripper]
    #1282620 - 02/05/03 03:50 PM (21 years, 1 month ago)

i believe it was because it was mixed in one bowl. try doing it jar by jar and i bet u anything ur problem will be solved.


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Offlineflawed
Stranger

Registered: 10/30/02
Posts: 87
Loc: Canada
Last seen: 21 years, 15 days
Re: colonize damn you! [Re: celestialtripper]
    #1282782 - 02/05/03 04:52 PM (21 years, 1 month ago)

wow, tons of contradicting and/or useless suggestions on this one .. heh, i've mixed my brown rice + vermiculite in one big bowl many many times. i usually do a dozen jars at a time and the substrate for the whole thing gets mixed in one big bowl. never had a problem. don't listen to people telling you to mix each jar separately. do you have any idea how long that would take? a dozen jars could easily take you 2hrs+ to do that way.

anyway, here are some of my guesses as to why you're not getting optimal growth;

your substrate is too dry. did you get the water to substrate ratio right?

your jars aren't getting enough air exchange. do you have the holes taped down?

you bought shitty syringes from a shitty online vendor. i've bought some syringes before which were absolutely useless. some vendors seem to be only concerned with squeezing as many syringes out of a print as possible. anyway, i've had your problem before because of this. ie. some jars would take forever to germinate and others wouldn't germinate at all.

hope this helps.


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Anonymous

Re: colonize damn you! [Re: flawed]
    #1282802 - 02/05/03 04:59 PM (21 years, 1 month ago)

Agreed, I had no problems with four strains, but one contamed to hell, all of it....

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OfflineAngry Mycologist
Spontaneouslycombusting

Registered: 11/24/02
Posts: 1,282
Loc: Galapagos
Last seen: 20 years, 10 months
Re: colonize damn you! [Re: celestialtripper]
    #1282807 - 02/05/03 05:01 PM (21 years, 1 month ago)

You still haven't mentioned your incubation temperature. This is crucial...


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The proper penalty of ignorance, which is of course that those who don't know should learn from those who do... - Plato

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Anonymous

Re: colonize damn you! [Re: Angry Mycologist]
    #1282813 - 02/05/03 05:03 PM (21 years, 1 month ago)

the man is correct :smile: 

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InvisibleSubGen1us

Registered: 11/26/02
Posts: 3,427
Re: colonize damn you! [Re: flawed]
    #1282857 - 02/05/03 05:20 PM (21 years, 1 month ago)

i did mention bacterial contam that could have stoped the growth. why not open a jar to check?

Quote:

your substrate is too dry. did you get the water to substrate ratio right?



he mixed em in one big bowl and half are growing ok. If the content was ok in the ones that grew they should be the same in the other jars SINCE they were mixed in one big bowl.
Quote:

your jars aren't getting enough air exchange. do you have the holes taped down?




um they havent even germinated so why are u asking this???

i see some pretty useless stuff here too man.


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Offlinef8L
Triumph

Registered: 09/07/02
Posts: 397
Last seen: 11 years, 1 day
Re: colonize damn you! [Re: SubGen1us]
    #1282864 - 02/05/03 05:24 PM (21 years, 1 month ago)

Here's my bet.

You did not shake the syringe, therefore the first several jars got the majority of the spores.


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Anonymous

Re: colonize damn you! [Re: celestialtripper]
    #1282873 - 02/05/03 05:27 PM (21 years, 1 month ago)

Perhaps it's a slow strain? We don't even know what he's using yet?
Correct?

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InvisibleSubGen1us

Registered: 11/26/02
Posts: 3,427
Re: colonize damn you! [Re: ]
    #1282888 - 02/05/03 05:31 PM (21 years, 1 month ago)

Quote:

Perhaps it's a slow strain? We don't even know what he's using yet?
Correct?




if u read erlier he stated half are at 80 % any cube strain will germinate within a month so that has nuthing to do with it.


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