Home | Community | Message Board


FreeSpores.com
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Agar, Brown Rice Flour, Malt Extract

Jump to first unread post. Pages: 1
OfflineH2O2shrooms
Couch Potato

Registered: 12/11/02
Posts: 67
Loc: in front of the computer
Last seen: 12 years, 6 months
Infinite Syringe?
    #1280667 - 02/05/03 07:34 AM (13 years, 11 months ago)

I got some stoner logic going, and want ya'll to poke some holes in this theory for me.

Heres the theory.

Find a nice mushroom I like, and skin a portion of it with a clean scalpel. and stick a syringe that is 1/2 full of Peroxide diluted to 6ml/Liter @ 3% into the mushrrom, and suck some myc into it, this failing open the syringe and drop some in.

Right so now suck some peroxide water with sugar (what ever you really like) into the syringe and wait. in a few days you should have a nice sterile growing bit of myc in a syringe. Do the normal shake and break routine to mix the myc in the syringe.

Use all but the last 1cc of the syringe, and repeat the previuos step. This should theoretically give you a single isolate to use until the unavoidable occurence of contams happens. So flaming the needle and always using peroxided solutions might be a few steps to take.

The thought process is as follows;

Isolates, what more do I need to say?

Live Innoc is always better than spore innoc as far as speed and (if clean) contams are concerned.

Honey/Liquid "teks" are a wonderful method of quickly starting jars due the former item.

Syringes are handy :smile:

Mycelium will survive a mild H2O2 bath while most contams cannot.

Senescense is not as apperant in agar cultures. This is likely due to number of cell divisions being the limiting factor on isolates.



--------------------
Mycology is a lot like quantum mechanics in that we don?t have causal relationships like in Newtonian physics, only probabilities of various outcomes.<=== Misapropriated from Mycofile


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineRaadt
nicht

Registered: 06/07/02
Posts: 2,104
Loc: azurescending
Last seen: 9 years, 9 days
Re: Infinite Syringe? [Re: H2O2shrooms]
    #1280700 - 02/05/03 07:47 AM (13 years, 11 months ago)

this is basically just a liquid inoculation, you could even expand this further, and use an eberbach or the 9er tek. Blend up that piece of stem, and suck it up. You don't even need to use sugar - as that would only allow contams, a bit of peroxide wouldnt hurt though.


--------------------
Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineH2O2shrooms
Couch Potato

Registered: 12/11/02
Posts: 67
Loc: in front of the computer
Last seen: 12 years, 6 months
Re: Infinite Syringe? [Re: Raadt]
    #1280733 - 02/05/03 08:01 AM (13 years, 11 months ago)

The sugar is required, with out it eventually you would run out of usable myc. I.E. doing liquid tek in the syringe, rather than transfer in/out of jars.


--------------------
Mycology is a lot like quantum mechanics in that we don?t have causal relationships like in Newtonian physics, only probabilities of various outcomes.<=== Misapropriated from Mycofile


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineBigJohnson
Whoa! You guysneed to lightenup!

Registered: 02/04/03
Posts: 716
Loc: The Yard
Last seen: 13 years, 6 months
Re: Infinite Syringe? [Re: H2O2shrooms]
    #1280793 - 02/05/03 08:23 AM (13 years, 11 months ago)

I like this stoner logic. I don't see why it wouldn't work, bro. Great idea.


--------------------
Should the US relegalize drugs?

http://www.lp.org/issues/relegalize.html


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineH2O2shrooms
Couch Potato

Registered: 12/11/02
Posts: 67
Loc: in front of the computer
Last seen: 12 years, 6 months
Re: Infinite Syringe? [Re: BigJohnson]
    #1280819 - 02/05/03 08:32 AM (13 years, 11 months ago)

Wait for it....
Wait for it....

I expect a hitch will arise from someones experience, I just am not sure what its gonna be cause I think I got the bases covered. But I am still new here :smile: 


--------------------
Mycology is a lot like quantum mechanics in that we don?t have causal relationships like in Newtonian physics, only probabilities of various outcomes.<=== Misapropriated from Mycofile


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinethe spiral
Neuroscientist
Male User Gallery

Folding@home Statistics
Registered: 05/13/02
Posts: 1,769
Last seen: 1 year, 6 months
Re: Infinite Syringe? [Re: H2O2shrooms]
    #1280901 - 02/05/03 08:59 AM (13 years, 11 months ago)

try it. when you say sugar I hope you mean dextrose or malt extract. the dextrose will be inviting contams, and peroxide won't make it invulnerable. also, the peroxide and the lack of air in the syringe may slow growth down. still, you may see some success - but you're running the risk of wasted jars or bags.


--------------------


"A celibate clergy is an especially good idea, because it tends to suppress any hereditary propensity toward fanaticism." - Carl Sagan


Edited by the spiral (02/05/03 09:01 AM)


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinedebianlinux
Myconerd - DBK
Male User Gallery

Folding@home Statistics
Registered: 12/09/02
Posts: 8,272
Loc: Over There
Last seen: 1 month, 14 days
Re: Infinite Syringe? [Re: the spiral]
    #1282178 - 02/05/03 03:38 PM (13 years, 11 months ago)

I keep an infinite running of mycelium syringes by starting spores in honey water and making syringes. the last syringe in the batch is used to start a new jar of HW ad nauseum.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineH2O2shrooms
Couch Potato

Registered: 12/11/02
Posts: 67
Loc: in front of the computer
Last seen: 12 years, 6 months
Re: Infinite Syringe? [Re: debianlinux]
    #1282311 - 02/05/03 04:17 PM (13 years, 11 months ago)

I am just thoerizing the jar step can be skipped. as for o2 there is a little bit introduced by the H2O2.


--------------------
Mycology is a lot like quantum mechanics in that we don?t have causal relationships like in Newtonian physics, only probabilities of various outcomes.<=== Misapropriated from Mycofile


Post Extras: Print Post  Remind Me! Notify Moderator
InvisiblePsychoslut
The Mother Fucking Bear-o-dactyl
 User Gallery
Registered: 12/10/02
Posts: 20,807
Loc: all up in ya
Re: Infinite Syringe? [Re: H2O2shrooms]
    #1282334 - 02/05/03 04:25 PM (13 years, 11 months ago)

mycelium isnt gonna be able to seperate h2o2 to make the o2 part useable


--------------------



[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinedebianlinux
Myconerd - DBK
Male User Gallery

Folding@home Statistics
Registered: 12/09/02
Posts: 8,272
Loc: Over There
Last seen: 1 month, 14 days
Re: Infinite Syringe? [Re: Psychoslut]
    #1282419 - 02/05/03 04:44 PM (13 years, 11 months ago)

hrrm, so exactly what DOES peroxidase do then?


Post Extras: Print Post  Remind Me! Notify Moderator
Invisible@cro
new name
 User Gallery

Registered: 12/07/02
Posts: 1,224
Loc: The PNW
Re: Infinite Syringe? [Re: Psychoslut]
    #1282863 - 02/05/03 07:23 PM (13 years, 11 months ago)

He's right H2O2 breaks down on its own into O2. It breaks down in heat, light you name it, it's not that stable. That's why it's kept in a dark bottle with a label that says "keep tightly closed, at room temperature and do not shake" The only reason it wouldn't convert to O2 is that it would be in an equilibrium: to convert from a liquid to a gas would create pressure on the syringe. I doubt the syringe would budge too much so very little O2 would be created. That's my 2 cents. - Later, @cro

Edit: I was being dumb, it probably would create sufficient O2 as it would just spit water out the needle until the same pressure is built up in the needle cover.


--------------------


Edited by @cro (02/05/03 09:03 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleIDontGrow
insomniac

Registered: 09/23/02
Posts: 217
Re: Infinite Syringe? [Re: @cro]
    #1282975 - 02/05/03 08:03 PM (13 years, 11 months ago)

only problem with cloneing is the rapid degeneratin that will occure when you clone the clone the clone..exc exc exc..you are going to have to start from spores again...and with a clone the tisue must be 100% contaim free and the chances of that are slim and none...unless your in a room with positive pressure..and thats unlikely...just build your self a nice positive pressure golve box and print 3-4 caps a run..that's more than sufficent for keeping it cheap and easy...unless your trying to make some kind of new super shroom..then go for it :wink:


--------------------
work of fiction

http://www.thenook.org/


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineH2O2shrooms
Couch Potato

Registered: 12/11/02
Posts: 67
Loc: in front of the computer
Last seen: 12 years, 6 months
Re: Infinite Syringe? [Re: IDontGrow]
    #1283059 - 02/05/03 08:50 PM (13 years, 11 months ago)

ahh but there is no cloning, see the original isolate is still alive in the syringe, and gets fed regularly. The degeneration you mention I beleive is senescence I mentioned, which the low number of cell divisions that a syringe goes through, would drastically delay compared to taking a new sample at every fruiting.

And as for contams while peroxide is damn good, yes care must be taken for any long term success. And once a syringe goes sour, you can clean it and resample the isolate still growing. This being done the isolate is now 1 full generation weaker than originally but I suspect under proper conditions a person could produce 1 orginism for a year, maybe more.


--------------------
Mycology is a lot like quantum mechanics in that we don?t have causal relationships like in Newtonian physics, only probabilities of various outcomes.<=== Misapropriated from Mycofile


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinesocratesmind
old hand

Registered: 02/22/02
Posts: 1,193
Loc: in your house :)
Last seen: 10 years, 6 months
Re: Infinite Syringe? [Re: H2O2shrooms]
    #1283065 - 02/05/03 08:56 PM (13 years, 11 months ago)

i do think degeneration occurs as only a few spores have to compete for the nutrients unnlike multispore into brf or whatever medium where the fastest best spores get the food ala survival of the fittest. oh yea for future ref my karo syringes i've done have always been slower than multispore.


--------------------
Prohibition will work great injury to the cause of temperance. It is a species of intemperance within itself, for it goes beyond the bounds of reason in that it attempts to control a man's appetite by legislation, and makes a crime out of things that are not crimes. A Prohibition law strikes a blow at the very principles upon which our government was founded.
- Abraham Lincoln: Speech in the Illinois House of Representatives, Dec 18, 1840.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineBaby_Hitler
Errorist
 User Gallery

Folding@home Statistics
Registered: 03/06/02
Posts: 22,840
Loc: To the limit!
Last seen: 3 months, 17 days
Re: Infinite Syringe? [Re: H2O2shrooms]
    #1283067 - 02/05/03 08:58 PM (13 years, 11 months ago)

You could keep the same culture around for years before it started loosing vigor.

If you get a really good culture, hang on to it. It's the "secret" to some of the best grow ops.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Invisible@cro
new name
 User Gallery

Registered: 12/07/02
Posts: 1,224
Loc: The PNW
Re: Infinite Syringe? [Re: socratesmind]
    #1283069 - 02/05/03 09:00 PM (13 years, 11 months ago)

He's not talking about spores he's saying he would use a clone which would be one set of genes and these genes would not degenerate because he will always be using the same genes. These genes will never change other than random mutations which is not to likely.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinedebianlinux
Myconerd - DBK
Male User Gallery

Folding@home Statistics
Registered: 12/09/02
Posts: 8,272
Loc: Over There
Last seen: 1 month, 14 days
Re: Infinite Syringe? [Re: @cro]
    #1283454 - 02/06/03 03:12 AM (13 years, 11 months ago)

Quote:

He's right H2O2 breaks down on its own into O2.




that is true as well, but what i was saying is that mycelium produces peroxidase, a compound whose function is to force h202 to break down.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineTaliesy
Peon
Registered: 01/27/03
Posts: 12
Loc: In ages past, when spells...
Last seen: 13 years, 6 months
Re: Infinite Syringe? [Re: H2O2shrooms]
    #1283584 - 02/06/03 05:13 AM (13 years, 11 months ago)

Quote:

ahh but there is no cloning, see the original isolate is still alive in the syringe, and gets fed regularly




perhaps someone can explain why this isn't cloning or at least clearly define cloning. My understanding of the term suggests that this is cloning.
Perhaps start a slant and a Malt Extract liquid culture. When degeneration occurs go back to the slant.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineBigJohnson
Whoa! You guysneed to lightenup!

Registered: 02/04/03
Posts: 716
Loc: The Yard
Last seen: 13 years, 6 months
Re: Infinite Syringe? [Re: Taliesy]
    #1283715 - 02/06/03 06:08 AM (13 years, 11 months ago)

After a few days, it can be placed in the fridge. Oxygen and nutrients won't be such an issue with cold storage.


--------------------
Should the US relegalize drugs?

http://www.lp.org/issues/relegalize.html


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineH2O2shrooms
Couch Potato

Registered: 12/11/02
Posts: 67
Loc: in front of the computer
Last seen: 12 years, 6 months
Re: Infinite Syringe? [Re: Taliesy]
    #1285283 - 02/06/03 01:44 PM (13 years, 11 months ago)

Sorry poor choice of words on my part. When you isolate a daikaryote all of its offspring are from the same genetics so yes it is a clone. The Issue brought up about multiplication of errors, senescence is again an issue but not as much of one as inoccing a jar with the isolate, spawning to bulk, and reisolating from there.

The following thread gives a very good explanation of what I am talking about and why growth in a syringe would not cause as rapid of ageing as the usual method of isolating does.

http://www.shroomery.org/forums/showflat.php?Cat=&Board=Forum4&Number=1258046&page=2&view=collapsed&sb=5&o=&fpart=1


--------------------
Mycology is a lot like quantum mechanics in that we don?t have causal relationships like in Newtonian physics, only probabilities of various outcomes.<=== Misapropriated from Mycofile


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Agar, Brown Rice Flour, Malt Extract

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* Dr Rush Wayne's Peroxide sterilisation process .. !!! InfiniteAdam 3,297 12 11/17/03 03:03 PM
by mycofile
* peroxide help - by mycofile Raadt 1,428 2 12/13/02 08:53 AM
by Azmodeus
* What is an effective use of Hydrogen Peroxide?
( 1 2 all )
garret 11,056 38 05/26/08 04:36 AM
by Tequila
* Unsteralized Peroxide Growing puwtrip 2,831 10 05/15/05 12:47 AM
by Snarp
* honey's natural peroxide? creamcorn 1,851 6 03/27/06 06:04 PM
by ALHOFF177A17
* Innoculating MEA with syring? upupup 1,004 5 10/29/02 12:30 PM
by upupup
* recipe for peroxide?
( 1 2 all )
sleepingbigguy 2,652 22 04/19/05 11:18 PM
by Abermelin
* Peroxidated grains MickyWilliams 1,919 13 01/18/06 12:27 PM
by mycofile

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Prisoner#1, RogerRabbit, EvilMushroom666
1,146 topic views. 1 members, 1 guests and 2 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Zamnesia.com
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.049 seconds spending 0.001 seconds on 14 queries.