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OfflineThePreed
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is air exchange crucial?
    #12787983 - 06/22/10 11:09 PM (13 years, 9 months ago)

As of now I have some jars with tape on the inoc' holes and some without. all have a generous dry verm cap. will this be enough to warn off contams? and will the jars with tape even grow any myc? is there a better method to keeps contams out and keep air flowing?

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InvisibleTacoHerder
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Re: is air exchange crucial? [Re: ThePreed]
    #12787989 - 06/22/10 11:11 PM (13 years, 9 months ago)

if the tape is micropore tape then you will be fine. if your have a verm layer you dont need the tape.


--------------------
TO ALL CULTIVATORS, EVERYTHING YOU NEED TO KNOW http://www.shroomery.org/forums/showflat.php/Number/10899385
We need more member like mycochef, he is truly a kind giving person. Just ask Noobie, mycochef hooked him up big time!
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InvisibleSgt. Frost
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Re: is air exchange crucial? [Re: TacoHerder]
    #12788023 - 06/22/10 11:19 PM (13 years, 9 months ago)

You're good with only the verm layer.

I'd suggest removing the tape on the others, there's no reason to keep them.

(Also, at this stage, you're not looking for air exchange, but gas exchange. Completely different.)

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OfflineBruit
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Re: is air exchange crucial? [Re: TacoHerder]
    #12788040 - 06/22/10 11:22 PM (13 years, 9 months ago)

gas exchange is in fact crucial during colonization. Actually remove the lids entirely, the dry verm layer (provided it's 1/2 inch thick ) will keep contaminant out and allow proper gas exchange. That would be the optimum.

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InvisibleTacoHerder
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Re: is air exchange crucial? [Re: Bruit]
    #12788054 - 06/22/10 11:25 PM (13 years, 9 months ago)

Quote:

Bruit said:
gas exchange is in fact crucial during colonization. Actually remove the lids entirely, the dry verm layer (provided it's 1/2 inch thick ) will keep contaminant out and allow proper gas exchange. That would be the optimum.



please dont do this, you want GE not air exchange, this would not only dry your cake out, but it would prob still contam.


--------------------
TO ALL CULTIVATORS, EVERYTHING YOU NEED TO KNOW http://www.shroomery.org/forums/showflat.php/Number/10899385
We need more member like mycochef, he is truly a kind giving person. Just ask Noobie, mycochef hooked him up big time!
Fastest Cultivation WINNER!!!! NoOneKnowsHowToAct won it twice, must be doing something right:super:

Shoot me a pm if you would like a powerful custom laser built. :smile: 4th pic is a 1.3W 445nm laser burning thru some plastic makeup containers. Yes I was wearing proper safety goggles, otherwise I would be blind right now.

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OfflineBruit
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Re: is air exchange crucial? [Re: Bruit]
    #12788073 - 06/22/10 11:30 PM (13 years, 9 months ago)

I'm going to say ignore my advice and listen to this guy instead.

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OfflineDamion5050
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Re: is air exchange crucial? [Re: Bruit]
    #12788135 - 06/22/10 11:42 PM (13 years, 9 months ago)

I am not sure if I would completely remove the lid cause that would allow to much GE and would basically be FAE.  And you don't want that till you start fruiting.  You want the CO2 levels to build up which aids in colonization.

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OfflineThePreed
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Re: is air exchange crucial? [Re: Damion5050]
    #12788170 - 06/22/10 11:48 PM (13 years, 9 months ago)

ok, tape is off of all jars. Ill also note that the jars are in a box that was sprayed with bleach disinfectant (some sprayed on the inoc' holes, ok or no?) and that the verm layer is more fine than the verm in the substrate which I read was a good thing.

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OfflineDamion5050
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Re: is air exchange crucial? [Re: ThePreed]
    #12788177 - 06/22/10 11:50 PM (13 years, 9 months ago)

IMO never spray bleach on anything especially your inoc holes.  Mycelium is very sensitive as well as the spores and if the bleach happened to get to the spores or young mycelium it will kill it.

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InvisibleJavadog
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Re: is air exchange crucial? [Re: Damion5050]
    #12788234 - 06/23/10 12:06 AM (13 years, 9 months ago)

I suppose alcohol would be a good alternative, as it is volatile.


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Invisiblejokefox
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Re: is air exchange crucial? [Re: Javadog]
    #12788260 - 06/23/10 12:11 AM (13 years, 9 months ago)

if your clean
you dont have to spray anything
i never have
just wash everything with soap and water

why battle the air when you can battle the surface witch the particles land on
i.e. dusting

em i the only one who dusts these days lol

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Offlinescatmanrav
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Re: is air exchange crucial? [Re: jokefox]
    #12788353 - 06/23/10 12:38 AM (13 years, 9 months ago)

Mycelium can actually tolerate bleach well. Should be fine.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay

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OfflineRogerRabbitM
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Re: is air exchange crucial? [Re: scatmanrav]
    #12789158 - 06/23/10 08:21 AM (13 years, 9 months ago)

It's a good idea prior to sterilization to tape the holes on a jar with micropore breathable tape, but once you inoculate, the needle pierces the hole and there's no need to repair it.  If you chose to put on another layer of tape, be sure to use micropore tape so gasses can still exchange.

Don't ever remove the lids, even if you have a verm layer.  The increase in air exchange, as opposed to 'gas' exchange will lead to premature pinning in the jars, often before colonization due to the loss of moisture.  The mycelium senses a threat because it's losing moisture, and the natural response is to reproduce before death.
RR


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OfflineThePreed
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Re: is air exchange crucial? [Re: RogerRabbit]
    #12791222 - 06/23/10 03:23 PM (13 years, 9 months ago)

I see. what if the inoc' holes were covered with non breathable tape while being PC'd? I figured the steam would over moisturize the substrate so I made sure it was sealed :/

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Offlinek00laid
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Re: is air exchange crucial? [Re: ThePreed]
    #12791256 - 06/23/10 03:27 PM (13 years, 9 months ago)

you were correct in taping the holes prior to sterilization.

but as previously stated in this thread. If you have the dry verm layer, do not bother re-taping the whole that you just inoculated through.


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OfflineBreakfast Crew
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Re: is air exchange crucial? [Re: k00laid]
    #12791284 - 06/23/10 03:32 PM (13 years, 9 months ago)

I hate tape.

So I slide one of these in before I put the lid on.


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InvisibleSgt. Frost
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Re: is air exchange crucial? [Re: ThePreed]
    #12791306 - 06/23/10 03:35 PM (13 years, 9 months ago)

Quote:

ThePreed said:
what if the inoc' holes were covered with non breathable tape while being PC'd? I figured the steam would over moisturize the substrate so I made sure it was sealed :/




That is fine. But from what it sounds like, you don't have a tinfoil cover on them while being PC'd. Do that or water will get in and shit will go down.

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Invisiblejokefox
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Re: is air exchange crucial? [Re: Sgt. Frost]
    #12791333 - 06/23/10 03:38 PM (13 years, 9 months ago)

i like to put tin foil over the lid then screw on the ring
its tight and neat

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OfflineThePreed
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Re: is air exchange crucial? [Re: Sgt. Frost]
    #12791647 - 06/23/10 04:44 PM (13 years, 9 months ago)

Quote:

Sgt. Frost said:
That is fine. But from what it sounds like, you don't have a tinfoil cover on them while being PC'd. Do that or water will get in and shit will go down.



I do not. but would the tape on the inoc' holes not serve the same purpose? its electrical tape. Im almost positive that air, water or light doesn't penetrate it (water from the steam beaded on it)

Quote:

Breakfast Crew said:
I hate tape.
So I slide one of these in before I put the lid on.
*pic*



are these muffin cups? would coffee filters work to keep out contams?

Edited by ThePreed (06/23/10 04:47 PM)

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InvisibleTacoHerder
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Re: is air exchange crucial? [Re: ThePreed]
    #12791666 - 06/23/10 04:48 PM (13 years, 9 months ago)

electrical tape doesnt work well once it gets wet.


--------------------
TO ALL CULTIVATORS, EVERYTHING YOU NEED TO KNOW http://www.shroomery.org/forums/showflat.php/Number/10899385
We need more member like mycochef, he is truly a kind giving person. Just ask Noobie, mycochef hooked him up big time!
Fastest Cultivation WINNER!!!! NoOneKnowsHowToAct won it twice, must be doing something right:super:

Shoot me a pm if you would like a powerful custom laser built. :smile: 4th pic is a 1.3W 445nm laser burning thru some plastic makeup containers. Yes I was wearing proper safety goggles, otherwise I would be blind right now.

Extras: Filter Print Post Top
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